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The map label for this gene is ubiE [H]

Identifier: 134300042

GI number: 134300042

Start: 2403746

End: 2404462

Strand: Reverse

Name: ubiE [H]

Synonym: Dred_2198

Alternate gene names: 134300042

Gene position: 2404462-2403746 (Counterclockwise)

Preceding gene: 134300043

Following gene: 134300041

Centisome position: 66.64

GC content: 41.84

Gene sequence:

>717_bases
GTGCAACAGTTTAATGGAAAAAATAAAGAAGAATTTGTGCATGGGGTATTTTCTACCATTGCCCACCGCTATGATCTAAT
GAATACTACCCTTAGCTTTAATAGAGATAAATACTGGAGAAGATATGCGGTCAAAGAAACGAACCTGAGACCCGGGGGTA
TGGCTCTGGATGTAGCCTGTGGCACAGGTATGTTATCCATTGAGTTAGCTAAGTTGGCAGGAACTTCCGGGAGGGTTGTG
GGGTTAGATTTTTGTGAAAACATGTTAGCCCATGCTGAAAGGAATATAGAAAAAACCCCCTATAAAAACAACATACAGTT
GATGCAGGGAAATGCCATGGAGTTACCCTTTGAAGACAACTTCTTTGACTGTGCTACCATTGGCTTTGCTCTAAGGAATG
TACCTAATATAGAAAAATGCATTGACGAAATGAGGCGGGTGGTGAAACCCGGGGGCAGAGTTATATCCCTTGAACTGGCT
AAGCCCAGTGCCCCGCTATTTAAGCAACTTTATTATCTGTATTTTGATCGTCTAGTCCCTTTATTAGGTAAACTTGGAGT
GGGCAAGGACGGTCCCTATCAGTGGCTTCCCAATTCTTTAAAATTATTTCCCCATCAATCCGTGATTCGAGATATATTTA
CCAAAGTGGGACTGAAGGATGCTATTTATCATGAACTAACCGGAGGTATCGTTGCTGTCCATGTAGGTATAAAGTAA

Upstream 100 bases:

>100_bases
CCATCTTGAAAAAACCATGTTGGAACAACTCCTTCATAGTTTATACTTGAACCAAGACATCATCCGTAAGGTTGTCTAAA
AAGAAGGGAGAACTACCGCA

Downstream 100 bases:

>100_bases
GTGCAAAAAAATTGTTTAGCAGAAATAATCCGAAGCATTAATAATTTAAATCTAATTAGGGATGGTGTGTTGTAAGTGGC
ATATCAAGATCTAAGGGATT

Product: ubiquinone/menaquinone biosynthesis methyltransferase

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MQQFNGKNKEEFVHGVFSTIAHRYDLMNTTLSFNRDKYWRRYAVKETNLRPGGMALDVACGTGMLSIELAKLAGTSGRVV
GLDFCENMLAHAERNIEKTPYKNNIQLMQGNAMELPFEDNFFDCATIGFALRNVPNIEKCIDEMRRVVKPGGRVISLELA
KPSAPLFKQLYYLYFDRLVPLLGKLGVGKDGPYQWLPNSLKLFPHQSVIRDIFTKVGLKDAIYHELTGGIVAVHVGIK

Sequences:

>Translated_238_residues
MQQFNGKNKEEFVHGVFSTIAHRYDLMNTTLSFNRDKYWRRYAVKETNLRPGGMALDVACGTGMLSIELAKLAGTSGRVV
GLDFCENMLAHAERNIEKTPYKNNIQLMQGNAMELPFEDNFFDCATIGFALRNVPNIEKCIDEMRRVVKPGGRVISLELA
KPSAPLFKQLYYLYFDRLVPLLGKLGVGKDGPYQWLPNSLKLFPHQSVIRDIFTKVGLKDAIYHELTGGIVAVHVGIK
>Mature_238_residues
MQQFNGKNKEEFVHGVFSTIAHRYDLMNTTLSFNRDKYWRRYAVKETNLRPGGMALDVACGTGMLSIELAKLAGTSGRVV
GLDFCENMLAHAERNIEKTPYKNNIQLMQGNAMELPFEDNFFDCATIGFALRNVPNIEKCIDEMRRVVKPGGRVISLELA
KPSAPLFKQLYYLYFDRLVPLLGKLGVGKDGPYQWLPNSLKLFPHQSVIRDIFTKVGLKDAIYHELTGGIVAVHVGIK

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) [H]

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Homo sapiens, GI116063536, Length=261, Percent_Identity=32.9501915708812, Blast_Score=146, Evalue=2e-35,
Organism=Escherichia coli, GI48994983, Length=237, Percent_Identity=35.4430379746835, Blast_Score=172, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17557039, Length=236, Percent_Identity=32.2033898305085, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6323526, Length=246, Percent_Identity=30.4878048780488, Blast_Score=115, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24641702, Length=250, Percent_Identity=28.4, Blast_Score=119, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26845; Mature: 26845

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: PS01183 UBIE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQFNGKNKEEFVHGVFSTIAHRYDLMNTTLSFNRDKYWRRYAVKETNLRPGGMALDVAC
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEEEC
GTGMLSIELAKLAGTSGRVVGLDFCENMLAHAERNIEKTPYKNNIQLMQGNAMELPFEDN
CCCCCEEEHHHHCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCEEEEECCEEECCCCCC
FFDCATIGFALRNVPNIEKCIDEMRRVVKPGGRVISLELAKPSAPLFKQLYYLYFDRLVP
CHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHH
LLGKLGVGKDGPYQWLPNSLKLFPHQSVIRDIFTKVGLKDAIYHELTGGIVAVHVGIK
HHHHCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHCHHHHHHHHHCCCEEEEEECCC
>Mature Secondary Structure
MQQFNGKNKEEFVHGVFSTIAHRYDLMNTTLSFNRDKYWRRYAVKETNLRPGGMALDVAC
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEEEC
GTGMLSIELAKLAGTSGRVVGLDFCENMLAHAERNIEKTPYKNNIQLMQGNAMELPFEDN
CCCCCEEEHHHHCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCEEEEECCEEECCCCCC
FFDCATIGFALRNVPNIEKCIDEMRRVVKPGGRVISLELAKPSAPLFKQLYYLYFDRLVP
CHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHH
LLGKLGVGKDGPYQWLPNSLKLFPHQSVIRDIFTKVGLKDAIYHELTGGIVAVHVGIK
HHHHCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHCHHHHHHHHHCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA