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The map label for this gene is prpE [H]

Identifier: 134300025

GI number: 134300025

Start: 2383320

End: 2384096

Strand: Reverse

Name: prpE [H]

Synonym: Dred_2179

Alternate gene names: 134300025

Gene position: 2384096-2383320 (Counterclockwise)

Preceding gene: 134300026

Following gene: 134300023

Centisome position: 66.08

GC content: 41.83

Gene sequence:

>777_bases
TTGAAAATCAACAGAAAAAATGAAAGGGGCCCCTTTGATATCATAGGCGATGTCCACGGTTGCCTAGAGGAATTAAGAGA
GCTTTTATATATCCTTGGTTACCGCGAGGAAAAGAAAAGCTATATACATCCGACGGGACGAAAGGCTATTTTTTTAGGTG
ATCTAGGTGATAGAGGGCCATACTGCGTGGAAAGTATTCGTTTGGTTATGGGGATGATTCATGGGGGCTACGGTCTGTAT
GTTCCTGGAAACCATTGTAATAAGCTATACAGATATCTTCAAGGCAGCAATGTACAGGTCAAGTATGGAATGGAAAAGAC
AGTGGCAGAATTGAAATCCCTAAATCCTATGGAAAGAGATATATTCTCCAGGGAGTTCATCTCCTTTTATGAAGCATCCC
CACTATACCTCATCTTGGACGCAGGAAATCTGGTGGCAACCCATGCTGGCATAAAGGAAGAAATGATTGGGCAGGTAAAC
AAGCGTATTAAAAGCTTCTGTCTTTATGGAGATACCACCGGAGATGTTACTCCAGAAGGTCTGCCGGTTCGTAGGGATTG
GGCCAAACATTATCGGGGTAAAGCGTTAATTGTTTATGGACATACCCCCGTTGAAGAGCCCGTTTTTAAAAATAATACTA
TTGATATCGATACTGCTTGTGCAATGGGAGGCAAGTTAACCGCATTAAGATATCCCGAGAGAAAAATTGTACAGGTACCA
GCTAGAACTGTTTACTACATAAGAGACGGTACAAAAATAGAAGTATCGATTGGCTAA

Upstream 100 bases:

>100_bases
GGACGACGATGATGATTTCTTTTAAAGAAACGTCCGGTTTGCCGGACGTTTTTCTAAGAAAATCTATCTTATGACTGCAA
TATAATAAGGAGGGGCTGTC

Downstream 100 bases:

>100_bases
ATTAGTTTATTTGAAAGGATAGATCTGCATCACAGAGAGGGCAAAATTTTAAGATTCCAGTGGCGGCAAATTCTTGGTTA
CATCGTTTACAGCAATAAAA

Product: metallophosphoesterase

Products: NA

Alternate protein names: Ap4A hydrolase; Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase; Diadenosine tetraphosphatase [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MKINRKNERGPFDIIGDVHGCLEELRELLYILGYREEKKSYIHPTGRKAIFLGDLGDRGPYCVESIRLVMGMIHGGYGLY
VPGNHCNKLYRYLQGSNVQVKYGMEKTVAELKSLNPMERDIFSREFISFYEASPLYLILDAGNLVATHAGIKEEMIGQVN
KRIKSFCLYGDTTGDVTPEGLPVRRDWAKHYRGKALIVYGHTPVEEPVFKNNTIDIDTACAMGGKLTALRYPERKIVQVP
ARTVYYIRDGTKIEVSIG

Sequences:

>Translated_258_residues
MKINRKNERGPFDIIGDVHGCLEELRELLYILGYREEKKSYIHPTGRKAIFLGDLGDRGPYCVESIRLVMGMIHGGYGLY
VPGNHCNKLYRYLQGSNVQVKYGMEKTVAELKSLNPMERDIFSREFISFYEASPLYLILDAGNLVATHAGIKEEMIGQVN
KRIKSFCLYGDTTGDVTPEGLPVRRDWAKHYRGKALIVYGHTPVEEPVFKNNTIDIDTACAMGGKLTALRYPERKIVQVP
ARTVYYIRDGTKIEVSIG
>Mature_258_residues
MKINRKNERGPFDIIGDVHGCLEELRELLYILGYREEKKSYIHPTGRKAIFLGDLGDRGPYCVESIRLVMGMIHGGYGLY
VPGNHCNKLYRYLQGSNVQVKYGMEKTVAELKSLNPMERDIFSREFISFYEASPLYLILDAGNLVATHAGIKEEMIGQVN
KRIKSFCLYGDTTGDVTPEGLPVRRDWAKHYRGKALIVYGHTPVEEPVFKNNTIDIDTACAMGGKLTALRYPERKIVQVP
ARTVYYIRDGTKIEVSIG

Specific function: Asymmetrically hydrolyzes Ap4p to yield AMP and ATP [H]

COG id: COG0639

COG function: function code T; Diadenosine tetraphosphatase and related serine/threonine protein phosphatases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR006186 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: =3.6.1.17 [H]

Molecular weight: Translated: 29215; Mature: 29215

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKINRKNERGPFDIIGDVHGCLEELRELLYILGYREEKKSYIHPTGRKAIFLGDLGDRGP
CCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCEEEEECCCCCCC
YCVESIRLVMGMIHGGYGLYVPGNHCNKLYRYLQGSNVQVKYGMEKTVAELKSLNPMERD
HHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCCEEEEECHHHHHHHHHHCCHHHHH
IFSREFISFYEASPLYLILDAGNLVATHAGIKEEMIGQVNKRIKSFCLYGDTTGDVTPEG
HHHHHHHHHHCCCCEEEEEECCCEEEECCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCC
LPVRRDWAKHYRGKALIVYGHTPVEEPVFKNNTIDIDTACAMGGKLTALRYPERKIVQVP
CCCHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEEHHHHHCCCEEEEEECCCCCEEECC
ARTVYYIRDGTKIEVSIG
CEEEEEEECCCEEEEEEC
>Mature Secondary Structure
MKINRKNERGPFDIIGDVHGCLEELRELLYILGYREEKKSYIHPTGRKAIFLGDLGDRGP
CCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCEEEEECCCCCCC
YCVESIRLVMGMIHGGYGLYVPGNHCNKLYRYLQGSNVQVKYGMEKTVAELKSLNPMERD
HHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCCEEEEECHHHHHHHHHHCCHHHHH
IFSREFISFYEASPLYLILDAGNLVATHAGIKEEMIGQVNKRIKSFCLYGDTTGDVTPEG
HHHHHHHHHHCCCCEEEEEECCCEEEECCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCC
LPVRRDWAKHYRGKALIVYGHTPVEEPVFKNNTIDIDTACAMGGKLTALRYPERKIVQVP
CCCHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEEHHHHHCCCEEEEEECCCCCEEECC
ARTVYYIRDGTKIEVSIG
CEEEEEEECCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA