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The map label for this gene is ilvK [H]

Identifier: 134299990

GI number: 134299990

Start: 2343826

End: 2344902

Strand: Reverse

Name: ilvK [H]

Synonym: Dred_2144

Alternate gene names: 134299990

Gene position: 2344902-2343826 (Counterclockwise)

Preceding gene: 134299991

Following gene: 134299989

Centisome position: 64.99

GC content: 40.48

Gene sequence:

>1077_bases
ATGAAAATTACAGTAGAACAGTTAGAAGGAAGCCAATTAAAACCCCTGTATCAAGATGATTCCCAGCTTGGTTTTGGTCG
CATATTTACCGATCGGATGTTTAGTATGCGGTTCGTCAATAATGCATGGAATGAGGCGCGTATTGAAAAATATAGATCCT
TTACTCTTGATCCATCTTCTTGCGTGTTTCACTATTCCCAGGAAATTTTTGAGGGATTGAAAGCCTATGCAGCACCGGAT
GGTAGAATTCTTCTTTTTAGACCTGATCAAAATGCACGAAGGATGAATCGGTCTGCAGAGCGCTTGGTCATGCCTACTAT
ACCAGAGGAAGATATCATACAGGCCATTGAAGAATTGGTGTTGGCAGAAAAAAGATGGCTACCCAAGCAACCGGGGACTT
CTATTTATATTAGACCCACTATGATTGCAACAGAAGCATTTTTGGGGGTTCATCCGTCTGCAGAGTATATTTTTTACGTT
ATTTTAAGTCCTGTGGGAGCTTATTATAAGGGTGGTTTTAAACCCATCAGTCTTTATGTTGAGGATACCTATGTAAGAGC
AAATGTGGGCGGTGTCGGAGATATTAAAACCGGCGGAAATTATGCGGCCAGTTTAATGGCAGGATACCGAGCCCAACAGA
AAGGTTTTTCTCAAGTTCTGTGGTTGGATGGCCGTGAGAGGAAATATATAGAAGAAGTTGGCGCTATGAATATGTTCTTT
GTTTATGGTAAAAAATTGGTAACTCCTAAACTCACAGGTTCGATCTTACCAGGTATTACCCGGGCGTCTGTATTAGAACT
TGCTCGATACTTAGGATTAGAGACCGAAGAAAGAATAATAAGTATTGACGATGTTATAGATGGAATTAGTTCGGGAGAAG
TTACCGAAGCCTTTGGTTCTGGGACGGCCGCTGTAATTTCTCCTGTGGGCTCTCTCTATTATAAAGAAAAGGATTTTGTT
ATTAACCATAACGAAGTTGGTTCCGTTACACAAACACTTTATGATAAATTGGTTAATATTCAATACGGCAGAGAGGAAGA
TCCCTTCGGTTGGGTAAGGGAAATTGGTAGATATTAA

Upstream 100 bases:

>100_bases
AAATATATTTTAGTCCATGCTAAAAGGATAATTAAACATGCAGGGAGAATTCTTAATGACAATTAACTCGGCTATGGTAT
AAAACGGGAGTGTGAACAAA

Downstream 100 bases:

>100_bases
AAATGAGTTATTATTTAAGTAGTGGTGCCATCTTCAGCTTTCCAAGGGGGCTTTAGCCTCCTTGGAAAAAATTAATGATT
AGCAGTGGCAAGAATTATAA

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT 2; Vegetative protein 85; VEG85 [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MKITVEQLEGSQLKPLYQDDSQLGFGRIFTDRMFSMRFVNNAWNEARIEKYRSFTLDPSSCVFHYSQEIFEGLKAYAAPD
GRILLFRPDQNARRMNRSAERLVMPTIPEEDIIQAIEELVLAEKRWLPKQPGTSIYIRPTMIATEAFLGVHPSAEYIFYV
ILSPVGAYYKGGFKPISLYVEDTYVRANVGGVGDIKTGGNYAASLMAGYRAQQKGFSQVLWLDGRERKYIEEVGAMNMFF
VYGKKLVTPKLTGSILPGITRASVLELARYLGLETEERIISIDDVIDGISSGEVTEAFGSGTAAVISPVGSLYYKEKDFV
INHNEVGSVTQTLYDKLVNIQYGREEDPFGWVREIGRY

Sequences:

>Translated_358_residues
MKITVEQLEGSQLKPLYQDDSQLGFGRIFTDRMFSMRFVNNAWNEARIEKYRSFTLDPSSCVFHYSQEIFEGLKAYAAPD
GRILLFRPDQNARRMNRSAERLVMPTIPEEDIIQAIEELVLAEKRWLPKQPGTSIYIRPTMIATEAFLGVHPSAEYIFYV
ILSPVGAYYKGGFKPISLYVEDTYVRANVGGVGDIKTGGNYAASLMAGYRAQQKGFSQVLWLDGRERKYIEEVGAMNMFF
VYGKKLVTPKLTGSILPGITRASVLELARYLGLETEERIISIDDVIDGISSGEVTEAFGSGTAAVISPVGSLYYKEKDFV
INHNEVGSVTQTLYDKLVNIQYGREEDPFGWVREIGRY
>Mature_358_residues
MKITVEQLEGSQLKPLYQDDSQLGFGRIFTDRMFSMRFVNNAWNEARIEKYRSFTLDPSSCVFHYSQEIFEGLKAYAAPD
GRILLFRPDQNARRMNRSAERLVMPTIPEEDIIQAIEELVLAEKRWLPKQPGTSIYIRPTMIATEAFLGVHPSAEYIFYV
ILSPVGAYYKGGFKPISLYVEDTYVRANVGGVGDIKTGGNYAASLMAGYRAQQKGFSQVLWLDGRERKYIEEVGAMNMFF
VYGKKLVTPKLTGSILPGITRASVLELARYLGLETEERIISIDDVIDGISSGEVTEAFGSGTAAVISPVGSLYYKEKDFV
INHNEVGSVTQTLYDKLVNIQYGREEDPFGWVREIGRY

Specific function: Transaminates branched-chain amino acids and ketoglutarate [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI296010904, Length=337, Percent_Identity=41.5430267062315, Blast_Score=265, Evalue=3e-71,
Organism=Homo sapiens, GI38176287, Length=337, Percent_Identity=41.5430267062315, Blast_Score=265, Evalue=4e-71,
Organism=Homo sapiens, GI296010906, Length=337, Percent_Identity=41.5430267062315, Blast_Score=265, Evalue=4e-71,
Organism=Homo sapiens, GI50658084, Length=329, Percent_Identity=39.8176291793313, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI296010902, Length=337, Percent_Identity=36.2017804154303, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI296010900, Length=337, Percent_Identity=36.2017804154303, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI258614015, Length=284, Percent_Identity=40.1408450704225, Blast_Score=212, Evalue=4e-55,
Organism=Escherichia coli, GI48994963, Length=321, Percent_Identity=29.9065420560748, Blast_Score=130, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17568601, Length=366, Percent_Identity=39.0710382513661, Blast_Score=249, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI17565728, Length=359, Percent_Identity=36.2116991643454, Blast_Score=191, Evalue=6e-49,
Organism=Saccharomyces cerevisiae, GI6322002, Length=339, Percent_Identity=44.5427728613569, Blast_Score=273, Evalue=2e-74,
Organism=Saccharomyces cerevisiae, GI6322608, Length=360, Percent_Identity=41.6666666666667, Blast_Score=260, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24641779, Length=361, Percent_Identity=39.8891966759003, Blast_Score=259, Evalue=2e-69,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 40387; Mature: 40387

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITVEQLEGSQLKPLYQDDSQLGFGRIFTDRMFSMRFVNNAWNEARIEKYRSFTLDPSS
CEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHH
CVFHYSQEIFEGLKAYAAPDGRILLFRPDQNARRMNRSAERLVMPTIPEEDIIQAIEELV
HHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHCCCHHHEECCCCCHHHHHHHHHHHH
LAEKRWLPKQPGTSIYIRPTMIATEAFLGVHPSAEYIFYVILSPVGAYYKGGFKPISLYV
HHHHHCCCCCCCCEEEEECHHHHHHHHHCCCCCHHEEEEEEEHHHHHHHCCCCEEEEEEE
EDTYVRANVGGVGDIKTGGNYAASLMAGYRAQQKGFSQVLWLDGRERKYIEEVGAMNMFF
EEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCEEE
VYGKKLVTPKLTGSILPGITRASVLELARYLGLETEERIISIDDVIDGISSGEVTEAFGS
ECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHEEEHHHHHCCCCCCCHHHHHCC
GTAAVISPVGSLYYKEKDFVINHNEVGSVTQTLYDKLVNIQYGREEDPFGWVREIGRY
CCHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCC
>Mature Secondary Structure
MKITVEQLEGSQLKPLYQDDSQLGFGRIFTDRMFSMRFVNNAWNEARIEKYRSFTLDPSS
CEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHH
CVFHYSQEIFEGLKAYAAPDGRILLFRPDQNARRMNRSAERLVMPTIPEEDIIQAIEELV
HHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHCCCHHHEECCCCCHHHHHHHHHHHH
LAEKRWLPKQPGTSIYIRPTMIATEAFLGVHPSAEYIFYVILSPVGAYYKGGFKPISLYV
HHHHHCCCCCCCCEEEEECHHHHHHHHHCCCCCHHEEEEEEEHHHHHHHCCCCEEEEEEE
EDTYVRANVGGVGDIKTGGNYAASLMAGYRAQQKGFSQVLWLDGRERKYIEEVGAMNMFF
EEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCEEE
VYGKKLVTPKLTGSILPGITRASVLELARYLGLETEERIISIDDVIDGISSGEVTEAFGS
ECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHEEEHHHHHCCCCCCCHHHHHCC
GTAAVISPVGSLYYKEKDFVINHNEVGSVTQTLYDKLVNIQYGREEDPFGWVREIGRY
CCHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 7934828; 9298659 [H]