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The map label for this gene is trmD

Identifier: 134299897

GI number: 134299897

Start: 2248767

End: 2249531

Strand: Reverse

Name: trmD

Synonym: Dred_2051

Alternate gene names: 134299897

Gene position: 2249531-2248767 (Counterclockwise)

Preceding gene: 134299898

Following gene: 134299896

Centisome position: 62.35

GC content: 44.31

Gene sequence:

>765_bases
ATGAGAATAGATATTCTGACACTTTTTCCTGAGATGTTTCAAGGACCGTTCAACCACAGTATTCTCAAGAGGGCCCAGGA
AAACAATCTTCTACAAATCGACACTATCAATATACGGGATTTTTCCCAAAACAAACACCATACAGTGGATGACACTCCCT
ATGGCGGTGGTGCAGGCATGGTGATGGGTCCGGAGCCTTTGTTTGAATGCTTTGATCACCTAAAAGCAAAGAACGCTGGT
CAGGTTGGCCGGGTAATCATGATGTGTCCCCAGGGGGAGCCCTTTACCCAAGAATATGCTAAGGAATTGGCCAGAGAAGA
AAATTTGGTTATTGTCTGTGGCCATTATGAGGGAATTGATGAAAGAGTTCGAGAAGTATTGGTAACGGATGAAATATCCA
TTGGTGACTATGTTCTCACCGGTGGTGAACTGCCTGCAATGGTGGTTGTTGATGCTGTGGCCAGGATGATTCCTGGCGTA
TTGGGTGAGACTGCCAGTGCTGAAGAAGACTCCTTTTATAACGGTCTGTTGGAGCATCCCCATTTTACTAAGCCCAGGGA
GTATAGAGGTTACGAAGTGCCTGAAATTCTACTGTCCGGTCACCATGGGAATATACGAAAGTGGCGCAGACGGCAGTCCC
TTCTAAGAACCCTAGAACGTCGACCAGAGTTACTAAAAGATGTAGAATTATCAAAGGAAGATAAGAAAGTTTTACTGGAA
CTGCAAAATCTTCTGTTATCTTTAAACCTAAAGCAGATGAAATAG

Upstream 100 bases:

>100_bases
TTAATTCCCGCCATTAAATCCGTTGTTAAGGAAATTAATATTACCGAAGGAAAAATGAAAGTCGAACTACTGGAAGGATT
AATATAAATAGGGTGAAAAC

Downstream 100 bases:

>100_bases
AAATTCTTGCCCAACAGGGCAGAATATGATATAATCTCAAATGTTGGTTAATTACGGACGGTCCGCTGCCTCTGGGAAAG
GTAAGAACGTCTAGGCGAGG

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MRIDILTLFPEMFQGPFNHSILKRAQENNLLQIDTINIRDFSQNKHHTVDDTPYGGGAGMVMGPEPLFECFDHLKAKNAG
QVGRVIMMCPQGEPFTQEYAKELAREENLVIVCGHYEGIDERVREVLVTDEISIGDYVLTGGELPAMVVVDAVARMIPGV
LGETASAEEDSFYNGLLEHPHFTKPREYRGYEVPEILLSGHHGNIRKWRRRQSLLRTLERRPELLKDVELSKEDKKVLLE
LQNLLLSLNLKQMK

Sequences:

>Translated_254_residues
MRIDILTLFPEMFQGPFNHSILKRAQENNLLQIDTINIRDFSQNKHHTVDDTPYGGGAGMVMGPEPLFECFDHLKAKNAG
QVGRVIMMCPQGEPFTQEYAKELAREENLVIVCGHYEGIDERVREVLVTDEISIGDYVLTGGELPAMVVVDAVARMIPGV
LGETASAEEDSFYNGLLEHPHFTKPREYRGYEVPEILLSGHHGNIRKWRRRQSLLRTLERRPELLKDVELSKEDKKVLLE
LQNLLLSLNLKQMK
>Mature_254_residues
MRIDILTLFPEMFQGPFNHSILKRAQENNLLQIDTINIRDFSQNKHHTVDDTPYGGGAGMVMGPEPLFECFDHLKAKNAG
QVGRVIMMCPQGEPFTQEYAKELAREENLVIVCGHYEGIDERVREVLVTDEISIGDYVLTGGELPAMVVVDAVARMIPGV
LGETASAEEDSFYNGLLEHPHFTKPREYRGYEVPEILLSGHHGNIRKWRRRQSLLRTLERRPELLKDVELSKEDKKVLLE
LQNLLLSLNLKQMK

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=243, Percent_Identity=50.2057613168724, Blast_Score=241, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_DESRM (A4J665)

Other databases:

- EMBL:   CP000612
- RefSeq:   YP_001113393.1
- ProteinModelPortal:   A4J665
- SMR:   A4J665
- STRING:   A4J665
- GeneID:   4957897
- GenomeReviews:   CP000612_GR
- KEGG:   drm:Dred_2051
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- PhylomeDB:   A4J665
- ProtClustDB:   PRK00026
- BioCyc:   DRED349161:DRED_2051-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 28964; Mature: 28964

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: BINDING 114-114

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIDILTLFPEMFQGPFNHSILKRAQENNLLQIDTINIRDFSQNKHHTVDDTPYGGGAGM
CEEEHEEHHHHHHCCCCCHHHHHHHHCCCEEEEEEEECEECCCCCCCCCCCCCCCCCCCC
VMGPEPLFECFDHLKAKNAGQVGRVIMMCPQGEPFTQEYAKELAREENLVIVCGHYEGID
CCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCHH
ERVREVLVTDEISIGDYVLTGGELPAMVVVDAVARMIPGVLGETASAEEDSFYNGLLEHP
HHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCC
HFTKPREYRGYEVPEILLSGHHGNIRKWRRRQSLLRTLERRPELLKDVELSKEDKKVLLE
CCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHH
LQNLLLSLNLKQMK
HHHHHHHCCHHHCC
>Mature Secondary Structure
MRIDILTLFPEMFQGPFNHSILKRAQENNLLQIDTINIRDFSQNKHHTVDDTPYGGGAGM
CEEEHEEHHHHHHCCCCCHHHHHHHHCCCEEEEEEEECEECCCCCCCCCCCCCCCCCCCC
VMGPEPLFECFDHLKAKNAGQVGRVIMMCPQGEPFTQEYAKELAREENLVIVCGHYEGID
CCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCHH
ERVREVLVTDEISIGDYVLTGGELPAMVVVDAVARMIPGVLGETASAEEDSFYNGLLEHP
HHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCC
HFTKPREYRGYEVPEILLSGHHGNIRKWRRRQSLLRTLERRPELLKDVELSKEDKKVLLE
CCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHH
LQNLLLSLNLKQMK
HHHHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA