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The map label for this gene is pnp

Identifier: 134299798

GI number: 134299798

Start: 2140299

End: 2142521

Strand: Reverse

Name: pnp

Synonym: Dred_1950

Alternate gene names: 134299798

Gene position: 2142521-2140299 (Counterclockwise)

Preceding gene: 134299799

Following gene: 134299797

Centisome position: 59.38

GC content: 43.59

Gene sequence:

>2223_bases
GTGTCTGAAAATCAAGTATTAATCAGGGAAATATCCTTGGGGGGAAGAACCCTCACTCTGGAAACAGGTCGAATGGCCAA
GCAGGCCAGTGGGGCTGTGCTGGTAACTTATGGAGAAACTGTTGTTCTGGTAACGGCTACAGTGGCTAAAAACACCCGGG
ATATTGATTTCTTCCCGCTAACAGTGGATTATGAAGAGAGACTCTATGCTGTTGGCAAAATCCCCGGAGGATTTATTAAA
AGGGAAGGTCGTCCTAGCGAAAAAGCAATCTTATCTGGTCGCTTAATTGACCGCCCAATTCGTCCACTTTTTCCTAAGCA
CATGAGAAACGAAGTGCAGGTAGTGGCCACAGTAATGTCAGTGGATCAGGATAATGCACCTGAAATAGCTGCTATGATTG
GGGCATCAGCCGCCTTACATATTTCCAAAATCCCCTTGAAAAAGCCCATTGGTGGGGTCATTGTTGGAAGAGTGGATGGA
CAGTTTGTCATTAATCCAATGGTTCGCCAAGCAGAAAACAGTGATATGCACCTTGTTGTGGCAGGCACAGATGATGCTGT
AATGATGGTTGAGGCAGGTGCCAAAGAAGTACCCGAGAGCGAAATGCTTGAAGGCATTATGTATGGACACGAAAAAGTAA
AAGAAATTGTTAAATTTATTGAAGACTTTAGAACAGAAGCTTTGGGCATGGGATTAGCCTTTGAGAAAATGGAGATTCCT
GAACCACAATTTGACTCCAATATGTCAGAAGCTATTTTAACAATTGCTGAAGAAGCAATTCGGGAAGCCGTTCTGCATTG
TTCTAGGGAGAAGCTAACCAAAAAGCAACGAGAAGTCTATATGGATGAGGTTATGTCAGGACTGCAAGAAAAATTCTTAG
AACAATTTCCTGAGAACCCTAAAGAAGTTACTATGTTGATTGAAAAGGCCGAAAAGAAAGTTGTCCGTCGGATAATTACC
CATGATAAACTTCGCATTGATGGTAGGGCAATCGATGAAATCCGCCCTATCTCAGTAGAAGTTGGGGTACTGCCACGTAC
CCATGGAACTGGATTGTTTACCAGAGGACAAACACAAATTCTGTCTGTTGCTACCCTAGGGTCCATTAGTGAAGAGCAGA
TATTAGATGGCCTTGGTGTTGAAGAAAGTAAGCGTTATATGCATCATTATAATTTCCCACCCTTTAGCACCGGTGAAACA
AAGCCCATGAGATCTCCTGGCCGTCGTGAAATTGGTCATGGTGCCTTGGCAGAAAGGGCACTGGAACCAATGATACCGCC
TGAAGAAGTATTCCCATATACCATTCGGGTGGTATCTGAAGCCATTGAATCAAATGGGTCTACATCAATGGGAAGTGTAT
GTGGCAGTACCCTTTCCTTAATGGATGCTGGTGTTCCATTAAAAGCACCTGTTGCTGGTGTAGCCATGGGGTTAATCATG
GAGGAAGATCAGTTTACTGTCCTCACTGATATTCAGGGCTTAGAAGACCACTTAGGGGATATGGACTTTAAAGTTGCTGG
ATCCGCCAATGGTGTTACGGCTCTCCAGATGGATATTAAAATCCCTGGTATCACCAGAGAAGTCTTTGAACAAGCTCTGG
AACAAGCCCATCGTGGCCGGATGTTTATTTTAGGAAAAATGTTGGAGGTTCTGTCTACTCCTAGAGCTGAAATCTCTGTG
CATGCTCCGGCCATTATCCGTACAAGTATCCATCCCGATAAAATCAGGGATATCATTGGACCTGGTGGCAAAATTATTAA
GAAATTGGTCGAAGAAACTGGTGCAGATATTGATATCGAGGATGATGGAAGAGTATTTATTGCGGCTGTTGACCGAGAGA
AGGGTAAACGGGCGTTGGAAATTATTCAGTCTATAACAGCCGAAGTTCAAGTTGGCAAGCTATACAATGGTAAAGTAACT
AGAGTAACCGATTTTGGTTGCTTTGTAGAAGTAATTCCCGGTGTTATGGGGCTACCAGGCAAAGAGGGACTGGTCCATAT
TTCACAGTTGGCTTTCCAAAGGGTTGAGAAAACCGAAGATATTGTTAAAGAAGGGGAAGTAATAGCCGTTAAAGCCATTG
GTTATGATCAACAGGGCAGGTTGAAACTTTCTAAAAAAGAAGCCATGAGGGATATGGGACTGGCACCAGCCGAAAGCACT
TCAGAACAACCAGAAAAAAGAGAACGAAGACCTTTTAGTCGTCCAAAAGCTACCAAGGAATAA

Upstream 100 bases:

>100_bases
AAATAAAGAACTTGTATTTTTCTCATATTAAGAAGGAAATACTATTAAAAATGTCGAATTGCTAAGATTTGTCAAGTACA
GAACTTTGGAGGTAAGAAGA

Downstream 100 bases:

>100_bases
AATCACATTAGCCGGTAAAATGGAGTTTTCTTTTGATTAAACTTCCGTTTGCCGGCTATATGTTTTTCGTGAGGGCTTAT
TATAAACAATATGGGCTAAA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 740; Mature: 739

Protein sequence:

>740_residues
MSENQVLIREISLGGRTLTLETGRMAKQASGAVLVTYGETVVLVTATVAKNTRDIDFFPLTVDYEERLYAVGKIPGGFIK
REGRPSEKAILSGRLIDRPIRPLFPKHMRNEVQVVATVMSVDQDNAPEIAAMIGASAALHISKIPLKKPIGGVIVGRVDG
QFVINPMVRQAENSDMHLVVAGTDDAVMMVEAGAKEVPESEMLEGIMYGHEKVKEIVKFIEDFRTEALGMGLAFEKMEIP
EPQFDSNMSEAILTIAEEAIREAVLHCSREKLTKKQREVYMDEVMSGLQEKFLEQFPENPKEVTMLIEKAEKKVVRRIIT
HDKLRIDGRAIDEIRPISVEVGVLPRTHGTGLFTRGQTQILSVATLGSISEEQILDGLGVEESKRYMHHYNFPPFSTGET
KPMRSPGRREIGHGALAERALEPMIPPEEVFPYTIRVVSEAIESNGSTSMGSVCGSTLSLMDAGVPLKAPVAGVAMGLIM
EEDQFTVLTDIQGLEDHLGDMDFKVAGSANGVTALQMDIKIPGITREVFEQALEQAHRGRMFILGKMLEVLSTPRAEISV
HAPAIIRTSIHPDKIRDIIGPGGKIIKKLVEETGADIDIEDDGRVFIAAVDREKGKRALEIIQSITAEVQVGKLYNGKVT
RVTDFGCFVEVIPGVMGLPGKEGLVHISQLAFQRVEKTEDIVKEGEVIAVKAIGYDQQGRLKLSKKEAMRDMGLAPAEST
SEQPEKRERRPFSRPKATKE

Sequences:

>Translated_740_residues
MSENQVLIREISLGGRTLTLETGRMAKQASGAVLVTYGETVVLVTATVAKNTRDIDFFPLTVDYEERLYAVGKIPGGFIK
REGRPSEKAILSGRLIDRPIRPLFPKHMRNEVQVVATVMSVDQDNAPEIAAMIGASAALHISKIPLKKPIGGVIVGRVDG
QFVINPMVRQAENSDMHLVVAGTDDAVMMVEAGAKEVPESEMLEGIMYGHEKVKEIVKFIEDFRTEALGMGLAFEKMEIP
EPQFDSNMSEAILTIAEEAIREAVLHCSREKLTKKQREVYMDEVMSGLQEKFLEQFPENPKEVTMLIEKAEKKVVRRIIT
HDKLRIDGRAIDEIRPISVEVGVLPRTHGTGLFTRGQTQILSVATLGSISEEQILDGLGVEESKRYMHHYNFPPFSTGET
KPMRSPGRREIGHGALAERALEPMIPPEEVFPYTIRVVSEAIESNGSTSMGSVCGSTLSLMDAGVPLKAPVAGVAMGLIM
EEDQFTVLTDIQGLEDHLGDMDFKVAGSANGVTALQMDIKIPGITREVFEQALEQAHRGRMFILGKMLEVLSTPRAEISV
HAPAIIRTSIHPDKIRDIIGPGGKIIKKLVEETGADIDIEDDGRVFIAAVDREKGKRALEIIQSITAEVQVGKLYNGKVT
RVTDFGCFVEVIPGVMGLPGKEGLVHISQLAFQRVEKTEDIVKEGEVIAVKAIGYDQQGRLKLSKKEAMRDMGLAPAEST
SEQPEKRERRPFSRPKATKE
>Mature_739_residues
SENQVLIREISLGGRTLTLETGRMAKQASGAVLVTYGETVVLVTATVAKNTRDIDFFPLTVDYEERLYAVGKIPGGFIKR
EGRPSEKAILSGRLIDRPIRPLFPKHMRNEVQVVATVMSVDQDNAPEIAAMIGASAALHISKIPLKKPIGGVIVGRVDGQ
FVINPMVRQAENSDMHLVVAGTDDAVMMVEAGAKEVPESEMLEGIMYGHEKVKEIVKFIEDFRTEALGMGLAFEKMEIPE
PQFDSNMSEAILTIAEEAIREAVLHCSREKLTKKQREVYMDEVMSGLQEKFLEQFPENPKEVTMLIEKAEKKVVRRIITH
DKLRIDGRAIDEIRPISVEVGVLPRTHGTGLFTRGQTQILSVATLGSISEEQILDGLGVEESKRYMHHYNFPPFSTGETK
PMRSPGRREIGHGALAERALEPMIPPEEVFPYTIRVVSEAIESNGSTSMGSVCGSTLSLMDAGVPLKAPVAGVAMGLIME
EDQFTVLTDIQGLEDHLGDMDFKVAGSANGVTALQMDIKIPGITREVFEQALEQAHRGRMFILGKMLEVLSTPRAEISVH
APAIIRTSIHPDKIRDIIGPGGKIIKKLVEETGADIDIEDDGRVFIAAVDREKGKRALEIIQSITAEVQVGKLYNGKVTR
VTDFGCFVEVIPGVMGLPGKEGLVHISQLAFQRVEKTEDIVKEGEVIAVKAIGYDQQGRLKLSKKEAMRDMGLAPAESTS
EQPEKRERRPFSRPKATKE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=716, Percent_Identity=35.4748603351955, Blast_Score=429, Evalue=1e-120,
Organism=Escherichia coli, GI145693187, Length=706, Percent_Identity=50.8498583569405, Blast_Score=657, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=716, Percent_Identity=31.145251396648, Blast_Score=332, Evalue=6e-91,
Organism=Drosophila melanogaster, GI281362905, Length=717, Percent_Identity=36.9595536959554, Blast_Score=434, Evalue=1e-121,
Organism=Drosophila melanogaster, GI24651641, Length=717, Percent_Identity=36.9595536959554, Blast_Score=434, Evalue=1e-121,
Organism=Drosophila melanogaster, GI24651643, Length=717, Percent_Identity=36.9595536959554, Blast_Score=434, Evalue=1e-121,
Organism=Drosophila melanogaster, GI161079377, Length=660, Percent_Identity=36.3636363636364, Blast_Score=398, Evalue=1e-111,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_DESRM (A4J5W6)

Other databases:

- EMBL:   CP000612
- RefSeq:   YP_001113294.1
- ProteinModelPortal:   A4J5W6
- SMR:   A4J5W6
- STRING:   A4J5W6
- GeneID:   4955875
- GenomeReviews:   CP000612_GR
- KEGG:   drm:Dred_1950
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- PhylomeDB:   A4J5W6
- ProtClustDB:   PRK11824
- BioCyc:   DRED349161:DRED_1950-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 81426; Mature: 81295

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENQVLIREISLGGRTLTLETGRMAKQASGAVLVTYGETVVLVTATVAKNTRDIDFFPL
CCCCCEEEEEECCCCEEEEEECCCHHHHCCCCEEEEECCEEEEEEEECCCCCCCCEEEEE
TVDYEERLYAVGKIPGGFIKREGRPSEKAILSGRLIDRPIRPLFPKHMRNEVQVVATVMS
EECHHHHEEEEECCCCHHHCCCCCCCHHHHHCCHHHCCCCCCCCCHHHHHHHHHHHHHHH
VDQDNAPEIAAMIGASAALHISKIPLKKPIGGVIVGRVDGQFVINPMVRQAENSDMHLVV
CCCCCCCHHHHHHCCHHHEEEECCCCCCCCCCEEEEECCCCEEECHHHHCCCCCCEEEEE
AGTDDAVMMVEAGAKEVPESEMLEGIMYGHEKVKEIVKFIEDFRTEALGMGLAFEKMEIP
ECCCCEEEEEECCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCC
EPQFDSNMSEAILTIAEEAIREAVLHCSREKLTKKQREVYMDEVMSGLQEKFLEQFPENP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
KEVTMLIEKAEKKVVRRIITHDKLRIDGRAIDEIRPISVEVGVLPRTHGTGLFTRGQTQI
HHHHHHHHHHHHHHHHHHHHCCCEEECCEECCCCCCEEEEEEEEECCCCCCEEECCCHHE
LSVATLGSISEEQILDGLGVEESKRYMHHYNFPPFSTGETKPMRSPGRREIGHGALAERA
EEEHHHCCCCHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHH
LEPMIPPEEVFPYTIRVVSEAIESNGSTSMGSVCGSTLSLMDAGVPLKAPVAGVAMGLIM
HCCCCCHHHHCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHEE
EEDQFTVLTDIQGLEDHLGDMDFKVAGSANGVTALQMDIKIPGITREVFEQALEQAHRGR
ECCCEEEEECCHHHHHHHCCCCEEEECCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCC
MFILGKMLEVLSTPRAEISVHAPAIIRTSIHPDKIRDIIGPGGKIIKKLVEETGADIDIE
EEHHHHHHHHHCCCCCEEEEECCEEEEECCCHHHHHHHHCCCHHHHHHHHHHCCCCEEEC
DDGRVFIAAVDREKGKRALEIIQSITAEVQVGKLYNGKVTRVTDFGCFVEVIPGVMGLPG
CCCCEEEEECCCCCCHHHHHHHHHHHHHEEECEEECCEEEEEECHHHHHHHHHHHHCCCC
KEGLVHISQLAFQRVEKTEDIVKEGEVIAVKAIGYDQQGRLKLSKKEAMRDMGLAPAEST
CCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEHHHHHHHHCCCCCCCCC
SEQPEKRERRPFSRPKATKE
CCCCHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SENQVLIREISLGGRTLTLETGRMAKQASGAVLVTYGETVVLVTATVAKNTRDIDFFPL
CCCCEEEEEECCCCEEEEEECCCHHHHCCCCEEEEECCEEEEEEEECCCCCCCCEEEEE
TVDYEERLYAVGKIPGGFIKREGRPSEKAILSGRLIDRPIRPLFPKHMRNEVQVVATVMS
EECHHHHEEEEECCCCHHHCCCCCCCHHHHHCCHHHCCCCCCCCCHHHHHHHHHHHHHHH
VDQDNAPEIAAMIGASAALHISKIPLKKPIGGVIVGRVDGQFVINPMVRQAENSDMHLVV
CCCCCCCHHHHHHCCHHHEEEECCCCCCCCCCEEEEECCCCEEECHHHHCCCCCCEEEEE
AGTDDAVMMVEAGAKEVPESEMLEGIMYGHEKVKEIVKFIEDFRTEALGMGLAFEKMEIP
ECCCCEEEEEECCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCC
EPQFDSNMSEAILTIAEEAIREAVLHCSREKLTKKQREVYMDEVMSGLQEKFLEQFPENP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
KEVTMLIEKAEKKVVRRIITHDKLRIDGRAIDEIRPISVEVGVLPRTHGTGLFTRGQTQI
HHHHHHHHHHHHHHHHHHHHCCCEEECCEECCCCCCEEEEEEEEECCCCCCEEECCCHHE
LSVATLGSISEEQILDGLGVEESKRYMHHYNFPPFSTGETKPMRSPGRREIGHGALAERA
EEEHHHCCCCHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHH
LEPMIPPEEVFPYTIRVVSEAIESNGSTSMGSVCGSTLSLMDAGVPLKAPVAGVAMGLIM
HCCCCCHHHHCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHEE
EEDQFTVLTDIQGLEDHLGDMDFKVAGSANGVTALQMDIKIPGITREVFEQALEQAHRGR
ECCCEEEEECCHHHHHHHCCCCEEEECCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCC
MFILGKMLEVLSTPRAEISVHAPAIIRTSIHPDKIRDIIGPGGKIIKKLVEETGADIDIE
EEHHHHHHHHHCCCCCEEEEECCEEEEECCCHHHHHHHHCCCHHHHHHHHHHCCCCEEEC
DDGRVFIAAVDREKGKRALEIIQSITAEVQVGKLYNGKVTRVTDFGCFVEVIPGVMGLPG
CCCCEEEEECCCCCCHHHHHHHHHHHHHEEECEEECCEEEEEECHHHHHHHHHHHHCCCC
KEGLVHISQLAFQRVEKTEDIVKEGEVIAVKAIGYDQQGRLKLSKKEAMRDMGLAPAEST
CCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEHHHHHHHHCCCCCCCCC
SEQPEKRERRPFSRPKATKE
CCCCHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA