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The map label for this gene is fumB [H]

Identifier: 134299724

GI number: 134299724

Start: 2057293

End: 2057847

Strand: Reverse

Name: fumB [H]

Synonym: Dred_1871

Alternate gene names: 134299724

Gene position: 2057847-2057293 (Counterclockwise)

Preceding gene: 134299725

Following gene: 134299723

Centisome position: 57.03

GC content: 45.05

Gene sequence:

>555_bases
ATGACAAAAGTTCATATATCAACACCCCTTGTGGAGGATGTTGTGAAAAGCCTTTGTATCGGTCAACAAGTCTTAATCAG
TGGGGTGATTTACACAGGGCGGGATGCAGCTCATAAAAGGCTAGTGCAACTGATGGATGAAGGGAAAGAACTACCCTTTG
ATTTGAAGGGACAGATTATTTATTATGTGGGTCCCTCACCGGCGCCTCCGGGTAGAGTAATTGGTTCTGCTGGGCCTACC
ACCGCAGGGCGAATGGATGCCTATGCACCCCGCTTGATAGAGCGGGGACTTAAGGGAATGATTGGTAAAGGTGCCAGGAC
AGAAAATGTAACCAATGCCATGAAGAAACATGGAGCTGTATACTTTGCTGCCATTGGTGGAGCGGCTGCCCTTATTTCAA
GAAGTGTGCAAGAGGCAGAAATTGTTGCTTATTCAGACTTAGGTCCAGAGGCTATCTATCGGTTAGTGGTAAAAGACTTT
CCGGCTATTGTTGTTAATGATGCCTTTGGTGGAGATTTATATCAAGAAGGTAGAAAAATATATGCGATAGAGTAA

Upstream 100 bases:

>100_bases
TATTGAAATATTTGGTTCTCACATTGCTAGTTTACCTGTAGCAGTTAACCTTAATTGCCATGCTGCAAGACATAAATCTG
TTGTTATTTAGGAGGATATG

Downstream 100 bases:

>100_bases
ACAAAGGTTTACTCTATCGCATTCCTTTCCCAATGTAAAAACACGAAACAAATCCTATTGTTGGCCGAAGCGAAAATAGT
AAACCCTGATAGTTATTTTA

Product: tartrate/fumarate subfamily Fe-S type hydro-lyase beta subunit

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 184; Mature: 183

Protein sequence:

>184_residues
MTKVHISTPLVEDVVKSLCIGQQVLISGVIYTGRDAAHKRLVQLMDEGKELPFDLKGQIIYYVGPSPAPPGRVIGSAGPT
TAGRMDAYAPRLIERGLKGMIGKGARTENVTNAMKKHGAVYFAAIGGAAALISRSVQEAEIVAYSDLGPEAIYRLVVKDF
PAIVVNDAFGGDLYQEGRKIYAIE

Sequences:

>Translated_184_residues
MTKVHISTPLVEDVVKSLCIGQQVLISGVIYTGRDAAHKRLVQLMDEGKELPFDLKGQIIYYVGPSPAPPGRVIGSAGPT
TAGRMDAYAPRLIERGLKGMIGKGARTENVTNAMKKHGAVYFAAIGGAAALISRSVQEAEIVAYSDLGPEAIYRLVVKDF
PAIVVNDAFGGDLYQEGRKIYAIE
>Mature_183_residues
TKVHISTPLVEDVVKSLCIGQQVLISGVIYTGRDAAHKRLVQLMDEGKELPFDLKGQIIYYVGPSPAPPGRVIGSAGPTT
AGRMDAYAPRLIERGLKGMIGKGARTENVTNAMKKHGAVYFAAIGGAAALISRSVQEAEIVAYSDLGPEAIYRLVVKDFP
AIVVNDAFGGDLYQEGRKIYAIE

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1838

COG function: function code C; Tartrate dehydratase beta subunit/Fumarate hydratase class I, C-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=178, Percent_Identity=43.2584269662921, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1787897, Length=178, Percent_Identity=41.0112359550562, Blast_Score=138, Evalue=2e-34,
Organism=Escherichia coli, GI1789443, Length=187, Percent_Identity=32.0855614973262, Blast_Score=99, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 19689; Mature: 19558

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKVHISTPLVEDVVKSLCIGQQVLISGVIYTGRDAAHKRLVQLMDEGKELPFDLKGQII
CCEEEECCHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCEE
YYVGPSPAPPGRVIGSAGPTTAGRMDAYAPRLIERGLKGMIGKGARTENVTNAMKKHGAV
EEECCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCE
YFAAIGGAAALISRSVQEAEIVAYSDLGPEAIYRLVVKDFPAIVVNDAFGGDLYQEGRKI
EEEEHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHCCEEEEECCCCCHHHHCCCEE
YAIE
EEEC
>Mature Secondary Structure 
TKVHISTPLVEDVVKSLCIGQQVLISGVIYTGRDAAHKRLVQLMDEGKELPFDLKGQII
CEEEECCHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCEE
YYVGPSPAPPGRVIGSAGPTTAGRMDAYAPRLIERGLKGMIGKGARTENVTNAMKKHGAV
EEECCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCE
YFAAIGGAAALISRSVQEAEIVAYSDLGPEAIYRLVVKDFPAIVVNDAFGGDLYQEGRKI
EEEEHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHCCEEEEECCCCCHHHHCCCEE
YAIE
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]