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The map label for this gene is pgaC [H]

Identifier: 134299450

GI number: 134299450

Start: 1733275

End: 1734552

Strand: Reverse

Name: pgaC [H]

Synonym: Dred_1593

Alternate gene names: 134299450

Gene position: 1734552-1733275 (Counterclockwise)

Preceding gene: 134299451

Following gene: 134299442

Centisome position: 48.07

GC content: 45.77

Gene sequence:

>1278_bases
ATGGTTAACACCATAACAGCAGATACACTTTTACACGGGATTGGTTATTTTACTTTTCTTTACCCTGTGACCATGAGTAT
CATTTGGGTCATTGGCGGTTTATATTTTTGGTGGTACCGAGAGAGAAAGGCGCGAACCAACAACTGGCCCGAGGTCTGGC
CCCCCGTAACCATTTTGATTCCCTGCCATAACGAGGAAATCAGCATTGCAACTACTTGCCATGCTTTAAGCAAAGTTAAT
TACCCAGACCTGAGAGTGGTCTTTATTGATGATGCTTCCACCGACAACACGGCACAGATCATCCGGGAATGGCTTAGGCA
AGAAGTTCCTTCTTTTCACCTATTACGGTTGACGACTAATCAGGGTAAGGCAAAGGCTTTAAACTGCGGCCTGCAAGTGG
CCGTACATACACCCATAACGGTTGTTATAGACGCTGATACTTTGATTACCCCCGATACCTTAAAATGGTTGATAGCCCCT
TTTATCCGCCAACCTCGTCTGGGTGCTGTCTCCGGAAATCCCCTGGTGGGAAATCGAGAAAATCTGCTAGAAAATTTACA
AACCGCGGAATTTGCTTCAATTCTAGGATTAATTAAGCGTTCTCAACGATCTCTGGGGCGGATGCTTACGGTATCTGGCT
GTATTACTGCCTTTTGTACCGATACCCTGAGGCAATTAGGTGGTTTCTCCTCCCGATCTGCCACAGAGGACATCGACATT
ACCTGGGCTATTCAGCGAAACTTTTATGAGGTATGGTTTGAACCAAGAGCAATAGCATATATTCAGGTACCCAAGACCAT
AAAGGAATTTTGGCACCAGAGGTGCCGTTGGGCCTTAGGAGGCTGGCACCTATTACGGAGCCATTGGGACATCTTTACCC
ACTGGCGGTGGCGCAGACTTTGGCCTGTTTATTTAGATTTTGTCATTAGTTATCTTTGGTCCTTTTGCTTGGTGATAGGA
ACTCTCCTATGGTTAGTTACCTATTTGATTCCTAGCAAACCTGCCATTGGCTTAACACCCATCCCAGCATGGTACGGTTC
TGTAATTAGTTTTGTTTGCCTGGTCCAATTCGGAGCAGCCCTATTAGCTAATCATCGCCATGATCACAAAATGTATCAAA
GTTTTTTCTGGATTCCCTGGTATCCTATCTTTTTCTTTTGCATAGGGGCTCTAACAGTAGTATGGACAAGCTGTCGGGGC
CTTTTCGGTGATCTGGTTACCGTTGGCAAATGGAAGAGTCCGGCCAGAAGTTCTATGCAGGGTTCGTTGGACGAGTAA

Upstream 100 bases:

>100_bases
AGAGGATCAAGTACCTCCTCTGCATTGGAACGAGGCAACCTTTGATCCCATGGATTTAAAATTTGAAATTAAGACGTAGC
CAAGAAAAGATGGTGAGGTA

Downstream 100 bases:

>100_bases
TATCCTATCGAATTGTTAGATACGGAAAACCCTTAGCCCGCCTCTTCGGTGGCTTGTACTTGAACCCTCCGCAATTCGTG
AGGGTTTCCCTTTCACGACA

Product: glycosyl transferase family protein

Products: NA

Alternate protein names: PGA synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm PGA synthesis protein PgaC; N-acetylglucosaminyltransferase PgaC [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MVNTITADTLLHGIGYFTFLYPVTMSIIWVIGGLYFWWYRERKARTNNWPEVWPPVTILIPCHNEEISIATTCHALSKVN
YPDLRVVFIDDASTDNTAQIIREWLRQEVPSFHLLRLTTNQGKAKALNCGLQVAVHTPITVVIDADTLITPDTLKWLIAP
FIRQPRLGAVSGNPLVGNRENLLENLQTAEFASILGLIKRSQRSLGRMLTVSGCITAFCTDTLRQLGGFSSRSATEDIDI
TWAIQRNFYEVWFEPRAIAYIQVPKTIKEFWHQRCRWALGGWHLLRSHWDIFTHWRWRRLWPVYLDFVISYLWSFCLVIG
TLLWLVTYLIPSKPAIGLTPIPAWYGSVISFVCLVQFGAALLANHRHDHKMYQSFFWIPWYPIFFFCIGALTVVWTSCRG
LFGDLVTVGKWKSPARSSMQGSLDE

Sequences:

>Translated_425_residues
MVNTITADTLLHGIGYFTFLYPVTMSIIWVIGGLYFWWYRERKARTNNWPEVWPPVTILIPCHNEEISIATTCHALSKVN
YPDLRVVFIDDASTDNTAQIIREWLRQEVPSFHLLRLTTNQGKAKALNCGLQVAVHTPITVVIDADTLITPDTLKWLIAP
FIRQPRLGAVSGNPLVGNRENLLENLQTAEFASILGLIKRSQRSLGRMLTVSGCITAFCTDTLRQLGGFSSRSATEDIDI
TWAIQRNFYEVWFEPRAIAYIQVPKTIKEFWHQRCRWALGGWHLLRSHWDIFTHWRWRRLWPVYLDFVISYLWSFCLVIG
TLLWLVTYLIPSKPAIGLTPIPAWYGSVISFVCLVQFGAALLANHRHDHKMYQSFFWIPWYPIFFFCIGALTVVWTSCRG
LFGDLVTVGKWKSPARSSMQGSLDE
>Mature_425_residues
MVNTITADTLLHGIGYFTFLYPVTMSIIWVIGGLYFWWYRERKARTNNWPEVWPPVTILIPCHNEEISIATTCHALSKVN
YPDLRVVFIDDASTDNTAQIIREWLRQEVPSFHLLRLTTNQGKAKALNCGLQVAVHTPITVVIDADTLITPDTLKWLIAP
FIRQPRLGAVSGNPLVGNRENLLENLQTAEFASILGLIKRSQRSLGRMLTVSGCITAFCTDTLRQLGGFSSRSATEDIDI
TWAIQRNFYEVWFEPRAIAYIQVPKTIKEFWHQRCRWALGGWHLLRSHWDIFTHWRWRRLWPVYLDFVISYLWSFCLVIG
TLLWLVTYLIPSKPAIGLTPIPAWYGSVISFVCLVQFGAALLANHRHDHKMYQSFFWIPWYPIFFFCIGALTVVWTSCRG
LFGDLVTVGKWKSPARSSMQGSLDE

Specific function: Probable N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N- acetylglucosamine to produce the linear homopolymer poly-beta-1,6- N-acetyl-D-glucosamine (PGA), a biofilm adhesin polysaccharide [H]

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787259, Length=414, Percent_Identity=35.7487922705314, Blast_Score=277, Evalue=1e-75,
Organism=Escherichia coli, GI1786560, Length=211, Percent_Identity=30.3317535545024, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 48929; Mature: 48929

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNTITADTLLHGIGYFTFLYPVTMSIIWVIGGLYFWWYRERKARTNNWPEVWPPVTILI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
PCHNEEISIATTCHALSKVNYPDLRVVFIDDASTDNTAQIIREWLRQEVPSFHLLRLTTN
ECCCCCEEHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEECC
QGKAKALNCGLQVAVHTPITVVIDADTLITPDTLKWLIAPFIRQPRLGAVSGNPLVGNRE
CCCCCEECCCEEEEEECCEEEEEECCCEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
NLLENLQTAEFASILGLIKRSQRSLGRMLTVSGCITAFCTDTLRQLGGFSSRSATEDIDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
TWAIQRNFYEVWFEPRAIAYIQVPKTIKEFWHQRCRWALGGWHLLRSHWDIFTHWRWRRL
EEEEECCCEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WPVYLDFVISYLWSFCLVIGTLLWLVTYLIPSKPAIGLTPIPAWYGSVISFVCLVQFGAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LLANHRHDHKMYQSFFWIPWYPIFFFCIGALTVVWTSCRGLFGDLVTVGKWKSPARSSMQ
HHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCC
GSLDE
CCCCC
>Mature Secondary Structure
MVNTITADTLLHGIGYFTFLYPVTMSIIWVIGGLYFWWYRERKARTNNWPEVWPPVTILI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
PCHNEEISIATTCHALSKVNYPDLRVVFIDDASTDNTAQIIREWLRQEVPSFHLLRLTTN
ECCCCCEEHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEECC
QGKAKALNCGLQVAVHTPITVVIDADTLITPDTLKWLIAPFIRQPRLGAVSGNPLVGNRE
CCCCCEECCCEEEEEECCEEEEEECCCEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
NLLENLQTAEFASILGLIKRSQRSLGRMLTVSGCITAFCTDTLRQLGGFSSRSATEDIDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
TWAIQRNFYEVWFEPRAIAYIQVPKTIKEFWHQRCRWALGGWHLLRSHWDIFTHWRWRRL
EEEEECCCEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WPVYLDFVISYLWSFCLVIGTLLWLVTYLIPSKPAIGLTPIPAWYGSVISFVCLVQFGAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LLANHRHDHKMYQSFFWIPWYPIFFFCIGALTVVWTSCRGLFGDLVTVGKWKSPARSSMQ
HHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCC
GSLDE
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]