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The map label for this gene is arsB [C]

Identifier: 134299330

GI number: 134299330

Start: 1596556

End: 1597830

Strand: Reverse

Name: arsB [C]

Synonym: Dred_1471

Alternate gene names: 134299330

Gene position: 1597830-1596556 (Counterclockwise)

Preceding gene: 134299332

Following gene: 134299327

Centisome position: 44.28

GC content: 43.14

Gene sequence:

>1275_bases
ATGAGTCAATTTACTTTAGCTTTAGTTATTTTTCTAATCACCTATGCATTCATCGTTTCAGAAAAGCTCCCTCGTACCGT
GGTCGCTCTGGCGGGGGCAGTGGTTGTACTCTTTAGTGGCATCGTTACCCAGGAAAAGGCCATTCATTACATTGATTGGA
ACACCATTGGTCTTTTAGTAGGAATGATGATTATTGTTTCTATTACCAGAAGAACCGGTGTTTTTGAATATTTAGCAGTT
AAATCCGCTGTAGCGGCAAAGGGTGACCCGCTGAAATTGTTGGTTCTACTGGCAGTCATTACGGCTGCTGCCTCTGCTCT
GCTGGATAATGTTACCACTGTATTGCTAATCGTTCCGGTAACCTTCTCCATTTGCCGTCAGTTGCAGGTAAATGTTGTTC
CCTTTTTGATTACTGAAATTATGGCCAGTAACATCGGTGGTACCTCAACCTTAATTGGTGACCCACCAAACATTATGATC
AGTGGTCCTGCTGGGTTGTCCTTTATGGATTTTGTTTATAATTTGGCTCCCATAGCAATTGTTATTTTCATTGTAACCAT
TACGATTCTACGGTTTATTTATCGTAAAGAACTAAAGGCAGATCCTGCTTTAATGGAAGAAATTACAAAGCTCAATCCCA
ATGACCAAATTAAAGATGCACCCCTGTTAAAGAAATCCCTCATTGCTTTATCCTTAACCATTGCTGGCTTTGCCCTGCAT
AGTGTTATTCATATGCCCACTGCTACCATTGCCATTGCCGGCGCAGTGTTATTACTATTAATGACCCGCGAAGAACCCGA
ACATGTTTTAGAAACAGTTGAATGGCCCACCATTTTCTTCTTCATTGGTTTGTTCATCGTAGTTGGTGGTTTGGAAGAGT
CTGGAGTTATTCACTGGATTGCTGAATCTGCTCTGCACTTTACCGGTGGGGAAGTATTTTCAACCGGTCTGTTGATACTC
TGGCTTTCTGCCATTGCATCAGCCTTTGTAGACAACATACCATTCGTAGCAACCATGATTCCTTTGTTACAACAAATGGG
TCAAATGGGTGGCATTACAAACCTTGATCCCCTGTGGTGGTCCCTGGCCCTGGGTGCTTGCCTGGGTGGTAACGGTACAC
TGGTGGGTGCCGCGGCGAACGTAATTGTTGCCGGTATGGCTGAAAAAAGAGGAACTCCCCTTAGTTTCCTTGGATTTATG
AAAATTGCTTTCCCCTTAATGATTCTGTCCATCGCGATTAGCACCATTTACCTTGTACTGTTCTTCCTCAGATAA

Upstream 100 bases:

>100_bases
TCTTTTTGTTTTTCCTAAATTTGCAAGGGGGTAGTCCGAGTCCAAAGGTTTTATTTTTATTGGGATTAACAAAGAATATC
TATAAGGAGGAATCAATTTA

Downstream 100 bases:

>100_bases
ACGGATAACTATACAGAGAATAACCGGGTTTGGCCCGGTTATTCTCTGTTAGAGTATATTCTTTAGATTAACCGCTTCAT
TCTCAGGAAGATGTAAAATC

Product: citrate transporter

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MSQFTLALVIFLITYAFIVSEKLPRTVVALAGAVVVLFSGIVTQEKAIHYIDWNTIGLLVGMMIIVSITRRTGVFEYLAV
KSAVAAKGDPLKLLVLLAVITAAASALLDNVTTVLLIVPVTFSICRQLQVNVVPFLITEIMASNIGGTSTLIGDPPNIMI
SGPAGLSFMDFVYNLAPIAIVIFIVTITILRFIYRKELKADPALMEEITKLNPNDQIKDAPLLKKSLIALSLTIAGFALH
SVIHMPTATIAIAGAVLLLLMTREEPEHVLETVEWPTIFFFIGLFIVVGGLEESGVIHWIAESALHFTGGEVFSTGLLIL
WLSAIASAFVDNIPFVATMIPLLQQMGQMGGITNLDPLWWSLALGACLGGNGTLVGAAANVIVAGMAEKRGTPLSFLGFM
KIAFPLMILSIAISTIYLVLFFLR

Sequences:

>Translated_424_residues
MSQFTLALVIFLITYAFIVSEKLPRTVVALAGAVVVLFSGIVTQEKAIHYIDWNTIGLLVGMMIIVSITRRTGVFEYLAV
KSAVAAKGDPLKLLVLLAVITAAASALLDNVTTVLLIVPVTFSICRQLQVNVVPFLITEIMASNIGGTSTLIGDPPNIMI
SGPAGLSFMDFVYNLAPIAIVIFIVTITILRFIYRKELKADPALMEEITKLNPNDQIKDAPLLKKSLIALSLTIAGFALH
SVIHMPTATIAIAGAVLLLLMTREEPEHVLETVEWPTIFFFIGLFIVVGGLEESGVIHWIAESALHFTGGEVFSTGLLIL
WLSAIASAFVDNIPFVATMIPLLQQMGQMGGITNLDPLWWSLALGACLGGNGTLVGAAANVIVAGMAEKRGTPLSFLGFM
KIAFPLMILSIAISTIYLVLFFLR
>Mature_423_residues
SQFTLALVIFLITYAFIVSEKLPRTVVALAGAVVVLFSGIVTQEKAIHYIDWNTIGLLVGMMIIVSITRRTGVFEYLAVK
SAVAAKGDPLKLLVLLAVITAAASALLDNVTTVLLIVPVTFSICRQLQVNVVPFLITEIMASNIGGTSTLIGDPPNIMIS
GPAGLSFMDFVYNLAPIAIVIFIVTITILRFIYRKELKADPALMEEITKLNPNDQIKDAPLLKKSLIALSLTIAGFALHS
VIHMPTATIAIAGAVLLLLMTREEPEHVLETVEWPTIFFFIGLFIVVGGLEESGVIHWIAESALHFTGGEVFSTGLLILW
LSAIASAFVDNIPFVATMIPLLQQMGQMGGITNLDPLWWSLALGACLGGNGTLVGAAANVIVAGMAEKRGTPLSFLGFMK
IAFPLMILSIAISTIYLVLFFLR

Specific function: Involved In Arsenical Resistance. Thought To Form The Channel Of An Arsenite Pump (By Similarity). [C]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CitM (TC 2.A.11) transporter family [H]

Homologues:

Organism=Homo sapiens, GI157266326, Length=502, Percent_Identity=33.4661354581673, Blast_Score=230, Evalue=2e-60,
Organism=Escherichia coli, GI87082277, Length=316, Percent_Identity=24.0506329113924, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787039, Length=341, Percent_Identity=26.099706744868, Blast_Score=73, Evalue=3e-14,
Organism=Drosophila melanogaster, GI28574683, Length=450, Percent_Identity=36.8888888888889, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI28574681, Length=450, Percent_Identity=36.8888888888889, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI28574685, Length=450, Percent_Identity=36.8888888888889, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI28574678, Length=450, Percent_Identity=36.8888888888889, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI19920704, Length=450, Percent_Identity=36.8888888888889, Blast_Score=246, Evalue=3e-65,
Organism=Drosophila melanogaster, GI24581759, Length=450, Percent_Identity=36.8888888888889, Blast_Score=246, Evalue=3e-65,
Organism=Drosophila melanogaster, GI221472711, Length=494, Percent_Identity=31.7813765182186, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI45550608, Length=469, Percent_Identity=33.6886993603412, Blast_Score=195, Evalue=5e-50,
Organism=Drosophila melanogaster, GI24581764, Length=494, Percent_Identity=31.7813765182186, Blast_Score=194, Evalue=1e-49,
Organism=Drosophila melanogaster, GI85724998, Length=470, Percent_Identity=25.9574468085106, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI62484377, Length=468, Percent_Identity=25.2136752136752, Blast_Score=152, Evalue=6e-37,
Organism=Drosophila melanogaster, GI24656008, Length=467, Percent_Identity=28.2655246252677, Blast_Score=136, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802
- InterPro:   IPR004680 [H]

Pfam domain/function: PF03600 CitMHS [H]

EC number: NA

Molecular weight: Translated: 45581; Mature: 45450

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQFTLALVIFLITYAFIVSEKLPRTVVALAGAVVVLFSGIVTQEKAIHYIDWNTIGLLV
CCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHH
GMMIIVSITRRTGVFEYLAVKSAVAAKGDPLKLLVLLAVITAAASALLDNVTTVLLIVPV
HHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFSICRQLQVNVVPFLITEIMASNIGGTSTLIGDPPNIMISGPAGLSFMDFVYNLAPIAI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHH
VIFIVTITILRFIYRKELKADPALMEEITKLNPNDQIKDAPLLKKSLIALSLTIAGFALH
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
SVIHMPTATIAIAGAVLLLLMTREEPEHVLETVEWPTIFFFIGLFIVVGGLEESGVIHWI
HHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCHHHHH
AESALHFTGGEVFSTGLLILWLSAIASAFVDNIPFVATMIPLLQQMGQMGGITNLDPLWW
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHH
SLALGACLGGNGTLVGAAANVIVAGMAEKRGTPLSFLGFMKIAFPLMILSIAISTIYLVL
HHHHHHHCCCCCCEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFLR
HHHC
>Mature Secondary Structure 
SQFTLALVIFLITYAFIVSEKLPRTVVALAGAVVVLFSGIVTQEKAIHYIDWNTIGLLV
CHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHH
GMMIIVSITRRTGVFEYLAVKSAVAAKGDPLKLLVLLAVITAAASALLDNVTTVLLIVPV
HHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFSICRQLQVNVVPFLITEIMASNIGGTSTLIGDPPNIMISGPAGLSFMDFVYNLAPIAI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHH
VIFIVTITILRFIYRKELKADPALMEEITKLNPNDQIKDAPLLKKSLIALSLTIAGFALH
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
SVIHMPTATIAIAGAVLLLLMTREEPEHVLETVEWPTIFFFIGLFIVVGGLEESGVIHWI
HHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCHHHHH
AESALHFTGGEVFSTGLLILWLSAIASAFVDNIPFVATMIPLLQQMGQMGGITNLDPLWW
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHH
SLALGACLGGNGTLVGAAANVIVAGMAEKRGTPLSFLGFMKIAFPLMILSIAISTIYLVL
HHHHHHHCCCCCCEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFLR
HHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]