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The map label for this gene is yeiE [H]

Identifier: 134299302

GI number: 134299302

Start: 1564994

End: 1565905

Strand: Reverse

Name: yeiE [H]

Synonym: Dred_1443

Alternate gene names: 134299302

Gene position: 1565905-1564994 (Counterclockwise)

Preceding gene: 134299306

Following gene: 134299300

Centisome position: 43.4

GC content: 36.95

Gene sequence:

>912_bases
ATGAACCTTTCTCATTTCAAGGTTTTTAAAATCCTTGCGCAAACTGAGAACATTACTAAGGCTAGCAAATTACTAGAATT
GTCTCAACCAACCATTAACCATAATATTTATTTACTCGAGCAACATTATGGTGCTAAACTGTTTGATCGATCCAATAAAA
AACTTAAACTAACTCGTTTTGGTGAAGTATTGGTGAAGTATGTTAATCAAATACTGAACCTCGTTGAGCAATCCGAGCAA
CACATTCATGAATTGGTGGGAGAACTTCGTGGAAGCCTGAATTTGGGGGCCAGTCATACCATTGCAGAAAATGTTTTGCC
AAAAATTATGGGTATGTTTAATCATGATTATCCAGAGGTTACCATTCATCTTGAAGTAACCAATACCCGTCACATTGTTG
ACCACATTTTAGATAATAAGTTGGATTTAGGACTTATTGAAGGACCTGTGACCCATGATAATATCATTTCAAAGCCCTTT
ATGGAGGATGAACTTCTCGTTGTTTTACCCTATAACCACCCCTTAGCAATTAAAAAGAATCTTACCATTAAAGAAGTTGC
CAACCTTCCCTTTGTTTTGAGAGAAGAAGGTTCCGGTACCCGAGTTGTTATGGAATCTACTCTAAAAGGTGCAGGCTTGG
ACCCTTCTGATCTAAATACTGTTATGGAGTTGGGTAATACACAGGCAGTTATCGGCGCCGTAGAAGCTGGTCTAGGAGCT
ACGATCTTATCGGGATTAGCAGTCAGTAAAGAAATACAGTTAAAAACCGTAAAGACCTGCCGAATTTCCAATGTAAATAT
CGTTCGCAACTTTTCAATTATTATCAACAAGGAAAAGCCATCTTCTTCGATTATTCAAACCTTTCTAGGCTTTATTAATT
CGGAAGAAATAAAAAAGATCTTTAAGGTATAA

Upstream 100 bases:

>100_bases
ATAAACTTTTTTCTTTTGTCAGCAATTTGGCAGACAAAAGTTGTTTTAGAGTTTAAAATAAGGAATATTAATCGTAATAG
GCAGAATAGTAGGTGATACC

Downstream 100 bases:

>100_bases
TTATTGTTTTATCAAAGGGCGTACCATTTAGGGTATGCCCTTTGAGTGTTAACTAAGTTGCAAAGGGATTTCCTTATCTC
TAAGGCGTAAAGATAAGGAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MNLSHFKVFKILAQTENITKASKLLELSQPTINHNIYLLEQHYGAKLFDRSNKKLKLTRFGEVLVKYVNQILNLVEQSEQ
HIHELVGELRGSLNLGASHTIAENVLPKIMGMFNHDYPEVTIHLEVTNTRHIVDHILDNKLDLGLIEGPVTHDNIISKPF
MEDELLVVLPYNHPLAIKKNLTIKEVANLPFVLREEGSGTRVVMESTLKGAGLDPSDLNTVMELGNTQAVIGAVEAGLGA
TILSGLAVSKEIQLKTVKTCRISNVNIVRNFSIIINKEKPSSSIIQTFLGFINSEEIKKIFKV

Sequences:

>Translated_303_residues
MNLSHFKVFKILAQTENITKASKLLELSQPTINHNIYLLEQHYGAKLFDRSNKKLKLTRFGEVLVKYVNQILNLVEQSEQ
HIHELVGELRGSLNLGASHTIAENVLPKIMGMFNHDYPEVTIHLEVTNTRHIVDHILDNKLDLGLIEGPVTHDNIISKPF
MEDELLVVLPYNHPLAIKKNLTIKEVANLPFVLREEGSGTRVVMESTLKGAGLDPSDLNTVMELGNTQAVIGAVEAGLGA
TILSGLAVSKEIQLKTVKTCRISNVNIVRNFSIIINKEKPSSSIIQTFLGFINSEEIKKIFKV
>Mature_303_residues
MNLSHFKVFKILAQTENITKASKLLELSQPTINHNIYLLEQHYGAKLFDRSNKKLKLTRFGEVLVKYVNQILNLVEQSEQ
HIHELVGELRGSLNLGASHTIAENVLPKIMGMFNHDYPEVTIHLEVTNTRHIVDHILDNKLDLGLIEGPVTHDNIISKPF
MEDELLVVLPYNHPLAIKKNLTIKEVANLPFVLREEGSGTRVVMESTLKGAGLDPSDLNTVMELGNTQAVIGAVEAGLGA
TILSGLAVSKEIQLKTVKTCRISNVNIVRNFSIIINKEKPSSSIIQTFLGFINSEEIKKIFKV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=299, Percent_Identity=32.1070234113712, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI1787879, Length=294, Percent_Identity=27.5510204081633, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI145693105, Length=246, Percent_Identity=28.0487804878049, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI157672245, Length=253, Percent_Identity=26.8774703557312, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1788748, Length=252, Percent_Identity=25.3968253968254, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1788887, Length=248, Percent_Identity=30.6451612903226, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1787530, Length=234, Percent_Identity=26.9230769230769, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787806, Length=244, Percent_Identity=25.4098360655738, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI87081904, Length=206, Percent_Identity=28.1553398058252, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1790399, Length=251, Percent_Identity=23.9043824701195, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787601, Length=171, Percent_Identity=25.1461988304094, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1789204, Length=294, Percent_Identity=22.7891156462585, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788296, Length=242, Percent_Identity=23.9669421487603, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790208, Length=245, Percent_Identity=24.0816326530612, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI87082132, Length=258, Percent_Identity=23.2558139534884, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790262, Length=249, Percent_Identity=24.4979919678715, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1788297, Length=254, Percent_Identity=22.4409448818898, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1786401, Length=157, Percent_Identity=23.5668789808917, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1790011, Length=289, Percent_Identity=24.9134948096886, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33797; Mature: 33797

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLSHFKVFKILAQTENITKASKLLELSQPTINHNIYLLEQHYGAKLFDRSNKKLKLTRF
CCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHCCCCCEEEEHHH
GEVLVKYVNQILNLVEQSEQHIHELVGELRGSLNLGASHTIAENVLPKIMGMFNHDYPEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCE
TIHLEVTNTRHIVDHILDNKLDLGLIEGPVTHDNIISKPFMEDELLVVLPYNHPLAIKKN
EEEEEECCHHHHHHHHHCCCCCCEEECCCCCCCHHHCCCCCCCCEEEEEECCCCEEEECC
LTIKEVANLPFVLREEGSGTRVVMESTLKGAGLDPSDLNTVMELGNTQAVIGAVEAGLGA
CCHHHHHCCCEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCHHH
TILSGLAVSKEIQLKTVKTCRISNVNIVRNFSIIINKEKPSSSIIQTFLGFINSEEIKKI
HHHHHHHHCCEEEEEEEEEEEECCCEEEEEEEEEEECCCCCHHHHHHHHHHCCHHHHHHH
FKV
HCC
>Mature Secondary Structure
MNLSHFKVFKILAQTENITKASKLLELSQPTINHNIYLLEQHYGAKLFDRSNKKLKLTRF
CCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHCCCCCEEEEHHH
GEVLVKYVNQILNLVEQSEQHIHELVGELRGSLNLGASHTIAENVLPKIMGMFNHDYPEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCE
TIHLEVTNTRHIVDHILDNKLDLGLIEGPVTHDNIISKPFMEDELLVVLPYNHPLAIKKN
EEEEEECCHHHHHHHHHCCCCCCEEECCCCCCCHHHCCCCCCCCEEEEEECCCCEEEECC
LTIKEVANLPFVLREEGSGTRVVMESTLKGAGLDPSDLNTVMELGNTQAVIGAVEAGLGA
CCHHHHHCCCEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCHHH
TILSGLAVSKEIQLKTVKTCRISNVNIVRNFSIIINKEKPSSSIIQTFLGFINSEEIKKI
HHHHHHHHCCEEEEEEEEEEEECCCEEEEEEEEEEECCCCCHHHHHHHHHHCCHHHHHHH
FKV
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]