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The map label for this gene is yyaE [H]

Identifier: 134299208

GI number: 134299208

Start: 1438358

End: 1440358

Strand: Reverse

Name: yyaE [H]

Synonym: Dred_1345

Alternate gene names: 134299208

Gene position: 1440358-1438358 (Counterclockwise)

Preceding gene: 134299209

Following gene: 134299201

Centisome position: 39.92

GC content: 44.38

Gene sequence:

>2001_bases
ATGCAGATAAAAACCAATTGCCCTTTAGATTGTTTTGCCCATTGTGGCCTAATCGCCCGTTTAGATAACGGGAAAGTAGT
TAGCATTTCCGGGGATGAAAGTCACCCGGTTAGTAAAGGTGTTATCTGTAGTAAAGGAAGAAATAAACACCTGCAGCGAC
TATATAGTCCTGAACGTATCACCACCCCCTTACGAAAAACAGCTGCTGGCTGGCAACCAATCACCTGGGACCAAGCCTAT
GAAACCATTGCTTTAGAATTATCCAAAATTATAAGTATTTATGGACCTTTAGCCATATTGCACCATGATAACGATGGTTC
CGAGGGGATTCTGAAAACACTTGCCGCAAGATTTTTTAATGCCCTCGGTGGCTGTACCCGTCCATCCGGTAGTATTTGCT
GGGGAAGCGGTTACCAAGCCCAGCAGTATGATTTTGGCCGGTTGCAATTACACAGTTGGGAAGATATGGTTAACTCCCGC
CTGATTGTTCTCTGGGGCAGAGATCCTGCCAGTACTAACATTCAAATGATACCACTTTTAAAACAGGCTAAAAAAAAGGG
CTGTCGAATTATCGTAATAAACCCTGTAAAGGTTGCTAGCTGTACTTTGGCTGATATTCATATAGCACCCCGACCGGGCA
GTGATGGTGCACTGGCCCTTGGTGTGGCACAGGAAATCATAAAGAACAACTGGACCGACCAAAACTACATTGAAAAACAC
GTATATGGTTATTCTCAATATGTTGAACTGGTGCAACGTTACACACCCGAAGTAGTCTCTGATATCACTGGTGTTTCCAT
CGACCAAATATGTTATCTGGCCCAGGCATATGCTAATGCCAAACCAGCCAGTATCTTTTTGGGCTACGGTTTACAACGTT
ATACCAACGGAGGGCAAACCATACGGGCCATTGATGCTCTGGCAGCCATTACCGGAAACATCGGTGTAGCAGGGAGCGGT
GTCAACTACGCCGGTGGTCATTGGAAAGGTTGCATTGCTTCTATTAAAGGTGATGAACTACCACAAAAAAATCGTACTCT
ACCCTGGCCTATTTTGGGAAAGGCTCTACAAAAAGCCGATAATCCACCTATAAAAAGCATCTTTGTAACTCGCTCTAACC
CTGTAACACAGTTGCCTGATACAACAGAAATTCGCAAAGCTTTTTCCCAATCGCAATTCACCGTTGTAGTTGATATGTTT
CTCAATGACACTGCCCAGTATGCTAATTTGTTTTTACCCTGTACAACTTTTCTTGAAGAAGAAGATCTGGTTTTTTCATC
TTGGAATCATTTTATGGCCTATTCACCACAGGTTGTGGAACCCTTAGGTCAGTGCAGATCAGATCACCATATCTTCTCAG
GGTTGGCCCAGCATATGGGTTTAACAGGTTTTCCCAACCTTACTGCCGAACAGTGGATAAAAAAAGCTATAAATCCATTG
AAACCTTTTAATATCACTCTGGAAAAACTTAAGAGCGGCCCTGTCAGACATCCCTTTGCCCCAGAAGTACCCTGGCAAGA
CGGTATCTTTGCTACTCCCAGTGGGAAATATGAGCTTTTTTCACAGTTGGCAGAGGCTGATGGCATTAATCCTTTGCCAG
AATATGTTGAGCCAATGGAAAGTGCCCTCAGGGATCCTATTTTAGCGAAGGAGTTCCCGCTTCATCTGATAACAGCACAC
CACCCGGAACACCTGCACTCACAATTCTGGAATCTTTCAGAAAAGGGACTGTCTATTCAACCGGTCTATATTCATCCAGA
AACGGCAGCTGTTGCTGGAATTATGGAAAACCATAAAGTTGTGGTGGCTACTCAAAGGGGTCAAGCTATTTTCACTGCTG
TCCTAAGTAAAAAACTTCGACGGGACACCATCCTGATTCACCAGGGTTCTTGGGCTATCCATGGCAACGGGGTAAATGTA
CTTACCCCCCAATACATGCCGGATATGGGACAAGGTACTCCCTACTATGATTGTCTGTGCAGAATAGAACCCCATCAATA
G

Upstream 100 bases:

>100_bases
CCAAGTTATTCTGCTAATCCCCAAAAACTGATCTGATGTAATTTAATAGCCGAAGTTTCGGCCATATTTTTATGCTACAA
TAATTTGGGAGGGACATTCC

Downstream 100 bases:

>100_bases
ATCCCTTTACCCCTCCACACCGAATCAGGTTCCCAGTAACTGGAACATACAGGACTACCCTGTTACCTATAAAAAACAAA
AACAAATTTAAGAGCAGTAA

Product: molybdopterin oxidoreductase

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: NA

Number of amino acids: Translated: 666; Mature: 666

Protein sequence:

>666_residues
MQIKTNCPLDCFAHCGLIARLDNGKVVSISGDESHPVSKGVICSKGRNKHLQRLYSPERITTPLRKTAAGWQPITWDQAY
ETIALELSKIISIYGPLAILHHDNDGSEGILKTLAARFFNALGGCTRPSGSICWGSGYQAQQYDFGRLQLHSWEDMVNSR
LIVLWGRDPASTNIQMIPLLKQAKKKGCRIIVINPVKVASCTLADIHIAPRPGSDGALALGVAQEIIKNNWTDQNYIEKH
VYGYSQYVELVQRYTPEVVSDITGVSIDQICYLAQAYANAKPASIFLGYGLQRYTNGGQTIRAIDALAAITGNIGVAGSG
VNYAGGHWKGCIASIKGDELPQKNRTLPWPILGKALQKADNPPIKSIFVTRSNPVTQLPDTTEIRKAFSQSQFTVVVDMF
LNDTAQYANLFLPCTTFLEEEDLVFSSWNHFMAYSPQVVEPLGQCRSDHHIFSGLAQHMGLTGFPNLTAEQWIKKAINPL
KPFNITLEKLKSGPVRHPFAPEVPWQDGIFATPSGKYELFSQLAEADGINPLPEYVEPMESALRDPILAKEFPLHLITAH
HPEHLHSQFWNLSEKGLSIQPVYIHPETAAVAGIMENHKVVVATQRGQAIFTAVLSKKLRRDTILIHQGSWAIHGNGVNV
LTPQYMPDMGQGTPYYDCLCRIEPHQ

Sequences:

>Translated_666_residues
MQIKTNCPLDCFAHCGLIARLDNGKVVSISGDESHPVSKGVICSKGRNKHLQRLYSPERITTPLRKTAAGWQPITWDQAY
ETIALELSKIISIYGPLAILHHDNDGSEGILKTLAARFFNALGGCTRPSGSICWGSGYQAQQYDFGRLQLHSWEDMVNSR
LIVLWGRDPASTNIQMIPLLKQAKKKGCRIIVINPVKVASCTLADIHIAPRPGSDGALALGVAQEIIKNNWTDQNYIEKH
VYGYSQYVELVQRYTPEVVSDITGVSIDQICYLAQAYANAKPASIFLGYGLQRYTNGGQTIRAIDALAAITGNIGVAGSG
VNYAGGHWKGCIASIKGDELPQKNRTLPWPILGKALQKADNPPIKSIFVTRSNPVTQLPDTTEIRKAFSQSQFTVVVDMF
LNDTAQYANLFLPCTTFLEEEDLVFSSWNHFMAYSPQVVEPLGQCRSDHHIFSGLAQHMGLTGFPNLTAEQWIKKAINPL
KPFNITLEKLKSGPVRHPFAPEVPWQDGIFATPSGKYELFSQLAEADGINPLPEYVEPMESALRDPILAKEFPLHLITAH
HPEHLHSQFWNLSEKGLSIQPVYIHPETAAVAGIMENHKVVVATQRGQAIFTAVLSKKLRRDTILIHQGSWAIHGNGVNV
LTPQYMPDMGQGTPYYDCLCRIEPHQ
>Mature_666_residues
MQIKTNCPLDCFAHCGLIARLDNGKVVSISGDESHPVSKGVICSKGRNKHLQRLYSPERITTPLRKTAAGWQPITWDQAY
ETIALELSKIISIYGPLAILHHDNDGSEGILKTLAARFFNALGGCTRPSGSICWGSGYQAQQYDFGRLQLHSWEDMVNSR
LIVLWGRDPASTNIQMIPLLKQAKKKGCRIIVINPVKVASCTLADIHIAPRPGSDGALALGVAQEIIKNNWTDQNYIEKH
VYGYSQYVELVQRYTPEVVSDITGVSIDQICYLAQAYANAKPASIFLGYGLQRYTNGGQTIRAIDALAAITGNIGVAGSG
VNYAGGHWKGCIASIKGDELPQKNRTLPWPILGKALQKADNPPIKSIFVTRSNPVTQLPDTTEIRKAFSQSQFTVVVDMF
LNDTAQYANLFLPCTTFLEEEDLVFSSWNHFMAYSPQVVEPLGQCRSDHHIFSGLAQHMGLTGFPNLTAEQWIKKAINPL
KPFNITLEKLKSGPVRHPFAPEVPWQDGIFATPSGKYELFSQLAEADGINPLPEYVEPMESALRDPILAKEFPLHLITAH
HPEHLHSQFWNLSEKGLSIQPVYIHPETAAVAGIMENHKVVVATQRGQAIFTAVLSKKLRRDTILIHQGSWAIHGNGVNV
LTPQYMPDMGQGTPYYDCLCRIEPHQ

Specific function: Terminal Reductase During Anaerobic Growth On Various Sulfoxide And N-Oxide Compounds. Allows E.Coli To Grow Anaerobically On Me(2)So As Respiratory Oxidant. [C]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=758, Percent_Identity=27.4406332453826, Blast_Score=227, Evalue=2e-60,
Organism=Escherichia coli, GI171474008, Length=759, Percent_Identity=26.3504611330698, Blast_Score=212, Evalue=7e-56,
Organism=Escherichia coli, GI1787870, Length=755, Percent_Identity=26.3576158940397, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI3868721, Length=711, Percent_Identity=25.7383966244726, Blast_Score=204, Evalue=2e-53,
Organism=Escherichia coli, GI87081994, Length=666, Percent_Identity=28.2282282282282, Blast_Score=182, Evalue=8e-47,
Organism=Escherichia coli, GI145693196, Length=661, Percent_Identity=26.1724659606657, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI1787231, Length=686, Percent_Identity=23.9067055393586, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788534, Length=518, Percent_Identity=23.9382239382239, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI3868719, Length=410, Percent_Identity=24.1463414634146, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787778, Length=319, Percent_Identity=23.8244514106583, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 73700; Mature: 73700

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIKTNCPLDCFAHCGLIARLDNGKVVSISGDESHPVSKGVICSKGRNKHLQRLYSPERI
CCCCCCCCHHHHHHCCEEEEECCCEEEEECCCCCCCCCCCCEECCCCCHHHHHHCCCHHH
TTPLRKTAAGWQPITWDQAYETIALELSKIISIYGPLAILHHDNDGSEGILKTLAARFFN
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHH
ALGGCTRPSGSICWGSGYQAQQYDFGRLQLHSWEDMVNSRLIVLWGRDPASTNIQMIPLL
HHCCCCCCCCCEEECCCCCCCCCCCCCEEEECHHHHHCCCEEEEECCCCCCCCEEEEHHH
KQAKKKGCRIIVINPVKVASCTLADIHIAPRPGSDGALALGVAQEIIKNNWTDQNYIEKH
HHHHHCCCEEEEECCCEEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHHHH
VYGYSQYVELVQRYTPEVVSDITGVSIDQICYLAQAYANAKPASIFLGYGLQRYTNGGQT
HCCHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEECCHHHHCCCCCC
IRAIDALAAITGNIGVAGSGVNYAGGHWKGCIASIKGDELPQKNRTLPWPILGKALQKAD
HHHHHHHHHHHCCCCEECCCCCCCCCCCHHHEEECCCCCCCCCCCCCCCHHHHHHHHHCC
NPPIKSIFVTRSNPVTQLPDTTEIRKAFSQSQFTVVVDMFLNDTAQYANLFLPCTTFLEE
CCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCEEEEEEEHHCCHHHHHHHHHHHHHHHCC
EDLVFSSWNHFMAYSPQVVEPLGQCRSDHHIFSGLAQHMGLTGFPNLTAEQWIKKAINPL
CCCEECCCCCHHCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCC
KPFNITLEKLKSGPVRHPFAPEVPWQDGIFATPSGKYELFSQLAEADGINPLPEYVEPME
CCCCHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHH
SALRDPILAKEFPLHLITAHHPEHLHSQFWNLSEKGLSIQPVYIHPETAAVAGIMENHKV
HHHHCCCCHHHCCEEEEECCCHHHHHHHHHCCHHCCCEEEEEEECCCHHHHHHHHCCCEE
VVATQRGQAIFTAVLSKKLRRDTILIHQGSWAIHGNGVNVLTPQYMPDMGQGTPYYDCLC
EEEECCCCHHHHHHHHHHHCCCEEEEECCCEEEECCCCEEECCCCCCCCCCCCCHHHEEE
RIEPHQ
EECCCC
>Mature Secondary Structure
MQIKTNCPLDCFAHCGLIARLDNGKVVSISGDESHPVSKGVICSKGRNKHLQRLYSPERI
CCCCCCCCHHHHHHCCEEEEECCCEEEEECCCCCCCCCCCCEECCCCCHHHHHHCCCHHH
TTPLRKTAAGWQPITWDQAYETIALELSKIISIYGPLAILHHDNDGSEGILKTLAARFFN
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHH
ALGGCTRPSGSICWGSGYQAQQYDFGRLQLHSWEDMVNSRLIVLWGRDPASTNIQMIPLL
HHCCCCCCCCCEEECCCCCCCCCCCCCEEEECHHHHHCCCEEEEECCCCCCCCEEEEHHH
KQAKKKGCRIIVINPVKVASCTLADIHIAPRPGSDGALALGVAQEIIKNNWTDQNYIEKH
HHHHHCCCEEEEECCCEEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHHHH
VYGYSQYVELVQRYTPEVVSDITGVSIDQICYLAQAYANAKPASIFLGYGLQRYTNGGQT
HCCHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEECCHHHHCCCCCC
IRAIDALAAITGNIGVAGSGVNYAGGHWKGCIASIKGDELPQKNRTLPWPILGKALQKAD
HHHHHHHHHHHCCCCEECCCCCCCCCCCHHHEEECCCCCCCCCCCCCCCHHHHHHHHHCC
NPPIKSIFVTRSNPVTQLPDTTEIRKAFSQSQFTVVVDMFLNDTAQYANLFLPCTTFLEE
CCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCEEEEEEEHHCCHHHHHHHHHHHHHHHCC
EDLVFSSWNHFMAYSPQVVEPLGQCRSDHHIFSGLAQHMGLTGFPNLTAEQWIKKAINPL
CCCEECCCCCHHCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCC
KPFNITLEKLKSGPVRHPFAPEVPWQDGIFATPSGKYELFSQLAEADGINPLPEYVEPME
CCCCHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHH
SALRDPILAKEFPLHLITAHHPEHLHSQFWNLSEKGLSIQPVYIHPETAAVAGIMENHKV
HHHHCCCCHHHCCEEEEECCCHHHHHHHHHCCHHCCCEEEEEEECCCHHHHHHHHCCCEE
VVATQRGQAIFTAVLSKKLRRDTILIHQGSWAIHGNGVNVLTPQYMPDMGQGTPYYDCLC
EEEECCCCHHHHHHHHHHHCCCEEEEECCCEEEECCCCEEECCCCCCCCCCCCCHHHEEE
RIEPHQ
EECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 1552862 [H]