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The map label for this gene is ltaA [H]

Identifier: 134297984

GI number: 134297984

Start: 110962

End: 111996

Strand: Reverse

Name: ltaA [H]

Synonym: Dred_0105

Alternate gene names: 134297984

Gene position: 111996-110962 (Counterclockwise)

Preceding gene: 134297986

Following gene: 134297972

Centisome position: 3.1

GC content: 47.25

Gene sequence:

>1035_bases
ATGATTACCATTCGAGTGGTAGATTTACGCAGTGACACCATCACTTTGCCCACTGACGAAATGAGAAAGGCCATGGCTAC
CGCGGTGGTTGGTGACGATGTATACAAAGAAGACCCTACGGTAAACCAGTTGGAAGAAACTGCCGCTTCTTTGCTTGGAA
AAGAAGCAGCTCTTTTTGTTCCTTCTGGAACCATGGGTAACCAAATAGCAGTTTTAACCCATACTGCCCGGGGTGAAGAG
GTCCTTCTGGACAGCGAAGCCCATATTTATTACTACGAAGTGGGCGGTCCTGCCATGCTGGCCGGGGTGCAATTAAGACC
GGTCAGTGATTTATTAACAGACCATGGCCCGGAAATTTTAAAGGGTTCCTTGCGGGGAGAAGATCTCCATTTTCCTCATA
CGTCATTAATTTGTTTAGAAAATACCTTTAATCGTGGTGGCGGCACCATCCTCTCTCCACACACCATGGGGAGGATTTAC
TCAATTGCCCAGGAACATAACCTAAAGGTCCACGTGGACGGTGCCCGTATTTTTAATGCAGCCGTATCCCTCGGGGTAGA
TGTTCGGACTTTTAGTCAGCATTGTGATTCCCTCATGTTCTGTCTTTCAAAGGGCTTGGCTGCACCAGTGGGTTCTTTGC
TGGTTGGTACACGGCAGTTTATCGAACGAGCAAGAAAATATCGCAAGGCTCTGGGGGGCGGCATGCGTCAAGCCGGTATT
CTGGCTGCCGCTGGTTTAGTTGCTTTGCAGAATGTGGACAGGCTTGCAGAAGATCATGCCAACGCCCGGCGGTTGGCCGA
AGGTCTGGCTAAATTACCGGGACTAGGTGTTGATTTGGCTAAAGTACAAACCAATATCATCGTTGTAAATGTAAATCAGC
CGCGCTCTGCAGCAGATTTAACCATGGCACTAAATAATAAAGGGATTAAATGTTCCACCTTTGGTCCCTCCACCATCCGC
ATGGTTACTCATATAAATGTTTCTAAAGAGGATATAGACTATGCCTTAGGCATTGCTAAATATCTCTTGCAATAA

Upstream 100 bases:

>100_bases
TTAGATATGGAAAGTTACTAAAAGATCCCTCCCTGGCAGAAAATAATTCAATCTATAGTATAATAAGTTTAAGAACTATT
TTTATATACTGCCTAAGGAG

Downstream 100 bases:

>100_bases
TAAAAAGGGCCGGTTTCCTTCCCCGGGAAAACCTGCCCTTTTTATTATCTTTTTAAAACTGGTCCATCATCTGGGTCACT
GCTTGGCCGTGGTGGCCTTC

Product: threonine aldolase

Products: glycine; acetaldehyde

Alternate protein names: L-allo-TA; L-allo-threonine acetaldehyde-lyase [H]

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MITIRVVDLRSDTITLPTDEMRKAMATAVVGDDVYKEDPTVNQLEETAASLLGKEAALFVPSGTMGNQIAVLTHTARGEE
VLLDSEAHIYYYEVGGPAMLAGVQLRPVSDLLTDHGPEILKGSLRGEDLHFPHTSLICLENTFNRGGGTILSPHTMGRIY
SIAQEHNLKVHVDGARIFNAAVSLGVDVRTFSQHCDSLMFCLSKGLAAPVGSLLVGTRQFIERARKYRKALGGGMRQAGI
LAAAGLVALQNVDRLAEDHANARRLAEGLAKLPGLGVDLAKVQTNIIVVNVNQPRSAADLTMALNNKGIKCSTFGPSTIR
MVTHINVSKEDIDYALGIAKYLLQ

Sequences:

>Translated_344_residues
MITIRVVDLRSDTITLPTDEMRKAMATAVVGDDVYKEDPTVNQLEETAASLLGKEAALFVPSGTMGNQIAVLTHTARGEE
VLLDSEAHIYYYEVGGPAMLAGVQLRPVSDLLTDHGPEILKGSLRGEDLHFPHTSLICLENTFNRGGGTILSPHTMGRIY
SIAQEHNLKVHVDGARIFNAAVSLGVDVRTFSQHCDSLMFCLSKGLAAPVGSLLVGTRQFIERARKYRKALGGGMRQAGI
LAAAGLVALQNVDRLAEDHANARRLAEGLAKLPGLGVDLAKVQTNIIVVNVNQPRSAADLTMALNNKGIKCSTFGPSTIR
MVTHINVSKEDIDYALGIAKYLLQ
>Mature_344_residues
MITIRVVDLRSDTITLPTDEMRKAMATAVVGDDVYKEDPTVNQLEETAASLLGKEAALFVPSGTMGNQIAVLTHTARGEE
VLLDSEAHIYYYEVGGPAMLAGVQLRPVSDLLTDHGPEILKGSLRGEDLHFPHTSLICLENTFNRGGGTILSPHTMGRIY
SIAQEHNLKVHVDGARIFNAAVSLGVDVRTFSQHCDSLMFCLSKGLAAPVGSLLVGTRQFIERARKYRKALGGGMRQAGI
LAAAGLVALQNVDRLAEDHANARRLAEGLAKLPGLGVDLAKVQTNIIVVNVNQPRSAADLTMALNNKGIKCSTFGPSTIR
MVTHINVSKEDIDYALGIAKYLLQ

Specific function: Stereospecifically catalyzes the interconversion of L- allo-threonine and glycine [H]

COG id: COG2008

COG function: function code E; Threonine aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1787095, Length=330, Percent_Identity=46.0606060606061, Blast_Score=282, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI193206520, Length=354, Percent_Identity=42.3728813559322, Blast_Score=253, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI193206522, Length=354, Percent_Identity=42.3728813559322, Blast_Score=253, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI193206524, Length=354, Percent_Identity=42.3728813559322, Blast_Score=253, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6320789, Length=312, Percent_Identity=39.4230769230769, Blast_Score=191, Evalue=2e-49,
Organism=Drosophila melanogaster, GI21355439, Length=373, Percent_Identity=43.6997319034853, Blast_Score=276, Evalue=2e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: 4.1.2.5

Molecular weight: Translated: 37004; Mature: 37004

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITIRVVDLRSDTITLPTDEMRKAMATAVVGDDVYKEDPTVNQLEETAASLLGKEAALFV
CEEEEEEEECCCEEECCHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHCCCCEEEE
PSGTMGNQIAVLTHTARGEEVLLDSEAHIYYYEVGGPAMLAGVQLRPVSDLLTDHGPEIL
CCCCCCCEEEEEEECCCCCEEEEECCCEEEEEECCCCEEEECEEECCHHHHHHCCCHHHH
KGSLRGEDLHFPHTSLICLENTFNRGGGTILSPHTMGRIYSIAQEHNLKVHVDGARIFNA
CCCCCCCCCCCCCCEEEEEECCCCCCCCEEECCHHHHHHHHHHHHCCCEEEECHHHHHHH
AVSLGVDVRTFSQHCDSLMFCLSKGLAAPVGSLLVGTRQFIERARKYRKALGGGMRQAGI
HHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LAAAGLVALQNVDRLAEDHANARRLAEGLAKLPGLGVDLAKVQTNIIVVNVNQPRSAADL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCHHE
TMALNNKGIKCSTFGPSTIRMVTHINVSKEDIDYALGIAKYLLQ
EEEECCCCEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MITIRVVDLRSDTITLPTDEMRKAMATAVVGDDVYKEDPTVNQLEETAASLLGKEAALFV
CEEEEEEEECCCEEECCHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHCCCCEEEE
PSGTMGNQIAVLTHTARGEEVLLDSEAHIYYYEVGGPAMLAGVQLRPVSDLLTDHGPEIL
CCCCCCCEEEEEEECCCCCEEEEECCCEEEEEECCCCEEEECEEECCHHHHHHCCCHHHH
KGSLRGEDLHFPHTSLICLENTFNRGGGTILSPHTMGRIYSIAQEHNLKVHVDGARIFNA
CCCCCCCCCCCCCCEEEEEECCCCCCCCEEECCHHHHHHHHHHHHCCCEEEECHHHHHHH
AVSLGVDVRTFSQHCDSLMFCLSKGLAAPVGSLLVGTRQFIERARKYRKALGGGMRQAGI
HHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LAAAGLVALQNVDRLAEDHANARRLAEGLAKLPGLGVDLAKVQTNIIVVNVNQPRSAADL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCHHE
TMALNNKGIKCSTFGPSTIRMVTHINVSKEDIDYALGIAKYLLQ
EEEECCCCEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 1.7

Km value (mM): 2.85 {L-Thr}} 0.24 {DL-erythro-phenylserine}} 0.22 {L-allo-Thr}} 0.12 {DL-threo-phenylserine}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = glycine + acetaldehyde

General reaction: Elimination of an aldehyde, C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9171400; 9228760 [H]