Click here to switch to the map view.

The map label for this gene is rpoA

Identifier: 13357817

GI number: 13357817

Start: 289719

End: 290702

Strand: Direct

Name: rpoA

Synonym: UU257

Alternate gene names: 13357817

Gene position: 289719-290702 (Clockwise)

Preceding gene: 13357816

Following gene: 13357818

Centisome position: 38.54

GC content: 30.28

Gene sequence:

>984_bases
ATGCGTAAATTTTTAAAATACCAACTTGATGTGCCATCAATTAATAGCGAAGACAAAAATCGTACAGTTGTTAAAATTGC
CCCACTTGAAATTGGTTTTGGTGACACATTAGGAAATGCCTTACGTCGAATTTGTTTATCATCAATTCCTGGTGCCTCAA
TGTTCGCTGTTAAATTTGGGGGATATTCGCATGAATTTCAACCATACGAAGGTGTTAAAGAAGACATTACTCACATCATT
TTAAACTTAAAAAATTTAGCAATTAAAATTGATGAATTAATTTATAGTGAAGACTATTTCAACAATTTATTAATTGATAA
ATGACCAAAAATGAAAATTAATTTCAAAGGACCTGGTGTGATTACAGCTAAAGATATTGTATGTCCAGTAGGTTTTGAAA
TTGTTAACCAAGACTTATATATTGCAGAAGTAACAAAACCAATTGATGTAGAAATTGAAATTTTTGCAAAAACAGGACGT
GGTCGTGTCGATTTTAATACAAATAAGGATTTTGTTTCAACACTACATATTATCGCAACCGATTCAAATTATTCACCAGT
TCTACATTACGCTTATAATGTAGAAATGATTAAAGATTCAAAATCATCAATGTCTGAGATTTTAACAATTGACATTGCAA
CAAATGGTACGATATCAGGAAGCGAAGCAATTGCGATTGCTGCTAAGATTATGCAAGCGCATTTAGAACCAATCGTGAAC
ATTGATAAAACAATTAATGAAATGATTATTATGCGTGAGCGTGAGGAAGAAGAAAAACGTCAAAACGCTTCAATTTCAAT
TGATGATTTAGATTTAACGGTGCGTGCATATAATGCTTTAAAACAATCAGGTATTAATACCACTGCTGAATTAATTGAAT
TAACAAAATCACAACTTGAAAAAATTAAGAATTTAGGACGTAAATCAGTTACTGAAATTATTCAAAAATTAACTGAACGT
TCACTTGAGTTAAAAAAAGATTAA

Upstream 100 bases:

>100_bases
AAAAATTAAAGATGTTACTCCAATTCCACATAATGGGTGTCGTCCACCTAAAAAACCAAGATAGTAATTTGGTTTTGATT
ATTGCAAAAGGAGAAATAAC

Downstream 100 bases:

>100_bases
AACTTTAAAAGAAAGGAATAATTTGATATGAGTTACATTAACAAACCAGGTAAAACACGCGCATGAAGAAAAATGGTGAG
TCGTCAACAAGTATCTGATG

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MRKFLKYQLDVPSINSEDKNRTVVKIAPLEIGFGDTLGNALRRICLSSIPGASMFAVKFGGYSHEFQPYEGVKEDITHII
LNLKNLAIKIDELIYSEDYFNNLLIDKWPKMKINFKGPGVITAKDIVCPVGFEIVNQDLYIAEVTKPIDVEIEIFAKTGR
GRVDFNTNKDFVSTLHIIATDSNYSPVLHYAYNVEMIKDSKSSMSEILTIDIATNGTISGSEAIAIAAKIMQAHLEPIVN
IDKTINEMIIMREREEEEKRQNASISIDDLDLTVRAYNALKQSGINTTAELIELTKSQLEKIKNLGRKSVTEIIQKLTER
SLELKKD

Sequences:

>Translated_327_residues
MRKFLKYQLDVPSINSEDKNRTVVKIAPLEIGFGDTLGNALRRICLSSIPGASMFAVKFGGYSHEFQPYEGVKEDITHII
LNLKNLAIKIDELIYSEDYFNNLLIDK*PKMKINFKGPGVITAKDIVCPVGFEIVNQDLYIAEVTKPIDVEIEIFAKTGR
GRVDFNTNKDFVSTLHIIATDSNYSPVLHYAYNVEMIKDSKSSMSEILTIDIATNGTISGSEAIAIAAKIMQAHLEPIVN
IDKTINEMIIMREREEEEKRQNASISIDDLDLTVRAYNALKQSGINTTAELIELTKSQLEKIKNLGRKSVTEIIQKLTER
SLELKKD
>Mature_327_residues
MRKFLKYQLDVPSINSEDKNRTVVKIAPLEIGFGDTLGNALRRICLSSIPGASMFAVKFGGYSHEFQPYEGVKEDITHII
LNLKNLAIKIDELIYSEDYFNNLLIDK*PKMKINFKGPGVITAKDIVCPVGFEIVNQDLYIAEVTKPIDVEIEIFAKTGR
GRVDFNTNKDFVSTLHIIATDSNYSPVLHYAYNVEMIKDSKSSMSEILTIDIATNGTISGSEAIAIAAKIMQAHLEPIVN
IDKTINEMIIMREREEEEKRQNASISIDDLDLTVRAYNALKQSGINTTAELIELTKSQLEKIKNLGRKSVTEIIQKLTER
SLELKKD

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family [H]

Homologues:

Organism=Escherichia coli, GI1789690, Length=333, Percent_Identity=37.2372372372372, Blast_Score=167, Evalue=8e-43,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- ProDom:   PD001179 [H]

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 36614; Mature: 36614

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKFLKYQLDVPSINSEDKNRTVVKIAPLEIGFGDTLGNALRRICLSSIPGASMFAVKFG
CCCCEEEEECCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEEEC
GYSHEFQPYEGVKEDITHIILNLKNLAIKIDELIYSEDYFNNLLIDKPKMKINFKGPGVI
CCCCCCCCCCCHHHHHHHHHHHHHHHEEEHHHHHHHHHHHCCEEEECCCEEEEECCCCEE
TAKDIVCPVGFEIVNQDLYIAEVTKPIDVEIEIFAKTGRGRVDFNTNKDFVSTLHIIATD
EHHCEECCCCHHHHCCCEEEEECCCCCCEEEEEEEECCCCEEECCCCHHHEEEEEEEEEC
SNYSPVLHYAYNVEMIKDSKSSMSEILTIDIATNGTISGSEAIAIAAKIMQAHLEPIVNI
CCCCCEEEEEEEEEEEECCHHHHHEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
DKTINEMIIMREREEEEKRQNASISIDDLDLTVRAYNALKQSGINTTAELIELTKSQLEK
HHHHHHHHHHHHHHHHHHHCCCCEEEECCEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
IKNLGRKSVTEIIQKLTERSLELKKD
HHHCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRKFLKYQLDVPSINSEDKNRTVVKIAPLEIGFGDTLGNALRRICLSSIPGASMFAVKFG
CCCCEEEEECCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEEEC
GYSHEFQPYEGVKEDITHIILNLKNLAIKIDELIYSEDYFNNLLIDKPKMKINFKGPGVI
CCCCCCCCCCCHHHHHHHHHHHHHHHEEEHHHHHHHHHHHCCEEEECCCEEEEECCCCEE
TAKDIVCPVGFEIVNQDLYIAEVTKPIDVEIEIFAKTGRGRVDFNTNKDFVSTLHIIATD
EHHCEECCCCHHHHCCCEEEEECCCCCCEEEEEEEECCCCEEECCCCHHHEEEEEEEEEC
SNYSPVLHYAYNVEMIKDSKSSMSEILTIDIATNGTISGSEAIAIAAKIMQAHLEPIVNI
CCCCCEEEEEEEEEEEECCHHHHHEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
DKTINEMIIMREREEEEKRQNASISIDDLDLTVRAYNALKQSGINTTAELIELTKSQLEK
HHHHHHHHHHHHHHHHHHHCCCCEEEECCEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
IKNLGRKSVTEIIQKLTERSLELKKD
HHHCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11048724 [H]