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The map label for this gene is xasA

Identifier: 13357782

GI number: 13357782

Start: 268049

End: 270328

Strand: Direct

Name: xasA

Synonym: UU223

Alternate gene names: 13357782

Gene position: 268049-270328 (Clockwise)

Preceding gene: 13357781

Following gene: 13357783

Centisome position: 35.66

GC content: 31.62

Gene sequence:

>2280_bases
ATGGGTTTTGTTTTACTAACAGCTTCAGCAATTATTGCTTTTTATACATTCCCATCACTTTCAACAGCTGGTTGAATTGC
AATTATCTTTGCTTTTATTACAGCTATGTGTTGATTCATTCCAATTGGGATTGCAGCCGCTGAAATGGCAACAATTAAAG
GTTGGGCCGAGGGGGGAATATTTACCTGGGTACGAAATTTATTAGGTCCACGAAATGGGTTTATTGTAACCTGATTACAA
TTCCAAGTCACATTTGGATTTGTGGCTATGATCTTATTCGTCCTTTCATCCTTTTCATTTGCTTTCGCAGGTGCTGATGG
ATATAATTATTTTAATACACTTAAGTTGGGAACAACTTTAAATCAAGCTACATCAGTCAACTTTTCAAAAAACTTTAGCA
ATGGGGCATTATATGGAATTGGTATTGTTGTTGTCGCAGGTCTAATCTTTATGAGTTTATTAGGCCAAAAACGAACTCAC
CAATTTGGCCAAGTTGCTTTAATCATTGGTATTTTACTACCATTCTTTATTATTTTAGGATTCCAAATTTATACAGTGGC
AACAATGCCAGATCCATTATACTTTATTGGAAAACACTTCCAAAAAGAACCACATTTAGACCCAAGTGTAACTGGTACTT
CTTATGAATTAAATAAAACTTTCATGTCATCAGTTGTTATGGCATCTTTCATGGCATTTAGTTTTAGCTTACATGGTGTA
GAAGTTTCAGCTATTGCTGCTAATCGAATGGATAATCCTTCTAAGAAATATCCACAAGCCATGATGATTGTTGTAATTTC
TGCTTTAGTTTGCATTATTATTGGATCAATTATGATTTCAATGACTGTACCAACTTCAACATTATCATTCAATGGTGGAT
TAGTACAGACAATTATGTTCAACTTATCTGTTGGTGGAATTGATGTTAAACTACCAGATGGCACAGTTGCTCATTATAAA
ACACTAGCACATGCATACTCAGTAATTGAAACTCAATATGGCACAGCTGCCGCTAACCAAGTTATGTATCAATTTGTGTA
TGGTCATCCAATTAATACAGGTAGTGACTTAGCACGTTCTGATTTATTAACACTAACATCAGAAGTTAACGATCCTTTAA
AAAACCCATCTTTATTTATTTCTGTTATGAATATAGAAACAAATAAAATGGCTATGCAAGGATTACAAGCTATTACTTTC
TTCATTGGAATAGGTGTATTTGTTGAAGTTTCTGTTTGAATGTCAAACCTTTCAACTGGATTAAATTATGCAATGCGTAA
AGCCCACTTCCCAGCATGGGTTACATATCGTTTAAAGAATGGGTCAGCGTTAACAGTAGCAATTTTATGAATTGTATTAC
TAATGTGCATTTTTGCAATTTACATGTTTGGTTATAATTCATTAATGCAATACAAAGGTCAAACAATTAGTGATATTTTA
GCTAATGAAATTCGTATTAACCAAGATCAAAAAGAACCAATGGCTTTATTGTCTAATCAAAATTTAGATGAAATTAAACT
AATGGCTCGTTCAATTTACGAACATAGTCATGCTCCTGGAGCACAAGCTAATGTTTTACATTTTGATTTTAATAAACATG
CTAACAGTGAAGTAAAAGCAATTTATGATAAAATCAAAGATTATATTGGCATTAATGCCGTAGCATCGGGAACACCAGCA
TCAATTACTAATATTTCATTCATTTCAAACGTTGTTTCACAAATCTCAATGTATTATATTGGATATAACATTTTCTTAAT
TGGATATCTAAGATATGTCTTTAAAGCTAATTGATTACGTCGTGAATTTAGAGTTAAATGATCATGATTACAAATTAGTT
TAGCTTTATTAGCAATGGGCGTTAATACATTTGCAGTTGTTGCAACTTACTTACCAGCTGCTCCAGAACTATATCCTGGA
CAAGAAGCTTATTATGTATTTATTTCAATTGCATTACCAATGTTTATATGCACATTATTACTTGGATTATTTATTTATCT
AATTAATTATGCTTGAAATAAAAAACGAGGTATTAATTTATCAGAACATTCAAACTTAACAAACGTTGATCAAAATGTTT
TAGATGTAATTAATAATTGACATTTATACAAAGATCAAAAAGAATTTGCTAAATTTGAACGTCATGTGCAATTATTACAA
CAAAAACAAGTAATTGTAAGAACAACAAAAGATAAGGCACAAAGAGAATTAGTTGCGAATGAAATGCAATATTTAGCAGC
TGAAATCGCAACAAGATTTGCACCAGAAGAAGAACATTAA

Upstream 100 bases:

>100_bases
AAATATTTTTAGAATAAGAGGTGATAAAAAATTGATAAATAATAAAATAAAAGAAGCAAAAATCAAAAAGAAACTAGAAA
AAAACAAAAAAGTTTCTTTA

Downstream 100 bases:

>100_bases
AAAACAAAAATGAGTCTTAATACTTTTTTAAAAAGTGTTTTGACTTATTTTTTTATTTTACACTTGACATATTATATATA
TATATATAATCTAAATGTAT

Product: amino acid antiporter

Products: L-glutamate [Cytoplasm]; 4-aminobutyrate [Periplasm] [C]

Alternate protein names: Extreme acid sensitivity protein [H]

Number of amino acids: Translated: 759; Mature: 758

Protein sequence:

>759_residues
MGFVLLTASAIIAFYTFPSLSTAGWIAIIFAFITAMCWFIPIGIAAAEMATIKGWAEGGIFTWVRNLLGPRNGFIVTWLQ
FQVTFGFVAMILFVLSSFSFAFAGADGYNYFNTLKLGTTLNQATSVNFSKNFSNGALYGIGIVVVAGLIFMSLLGQKRTH
QFGQVALIIGILLPFFIILGFQIYTVATMPDPLYFIGKHFQKEPHLDPSVTGTSYELNKTFMSSVVMASFMAFSFSLHGV
EVSAIAANRMDNPSKKYPQAMMIVVISALVCIIIGSIMISMTVPTSTLSFNGGLVQTIMFNLSVGGIDVKLPDGTVAHYK
TLAHAYSVIETQYGTAAANQVMYQFVYGHPINTGSDLARSDLLTLTSEVNDPLKNPSLFISVMNIETNKMAMQGLQAITF
FIGIGVFVEVSVWMSNLSTGLNYAMRKAHFPAWVTYRLKNGSALTVAILWIVLLMCIFAIYMFGYNSLMQYKGQTISDIL
ANEIRINQDQKEPMALLSNQNLDEIKLMARSIYEHSHAPGAQANVLHFDFNKHANSEVKAIYDKIKDYIGINAVASGTPA
SITNISFISNVVSQISMYYIGYNIFLIGYLRYVFKANWLRREFRVKWSWLQISLALLAMGVNTFAVVATYLPAAPELYPG
QEAYYVFISIALPMFICTLLLGLFIYLINYAWNKKRGINLSEHSNLTNVDQNVLDVINNWHLYKDQKEFAKFERHVQLLQ
QKQVIVRTTKDKAQRELVANEMQYLAAEIATRFAPEEEH

Sequences:

>Translated_759_residues
MGFVLLTASAIIAFYTFPSLSTAG*IAIIFAFITAMC*FIPIGIAAAEMATIKGWAEGGIFTWVRNLLGPRNGFIVT*LQ
FQVTFGFVAMILFVLSSFSFAFAGADGYNYFNTLKLGTTLNQATSVNFSKNFSNGALYGIGIVVVAGLIFMSLLGQKRTH
QFGQVALIIGILLPFFIILGFQIYTVATMPDPLYFIGKHFQKEPHLDPSVTGTSYELNKTFMSSVVMASFMAFSFSLHGV
EVSAIAANRMDNPSKKYPQAMMIVVISALVCIIIGSIMISMTVPTSTLSFNGGLVQTIMFNLSVGGIDVKLPDGTVAHYK
TLAHAYSVIETQYGTAAANQVMYQFVYGHPINTGSDLARSDLLTLTSEVNDPLKNPSLFISVMNIETNKMAMQGLQAITF
FIGIGVFVEVSV*MSNLSTGLNYAMRKAHFPAWVTYRLKNGSALTVAIL*IVLLMCIFAIYMFGYNSLMQYKGQTISDIL
ANEIRINQDQKEPMALLSNQNLDEIKLMARSIYEHSHAPGAQANVLHFDFNKHANSEVKAIYDKIKDYIGINAVASGTPA
SITNISFISNVVSQISMYYIGYNIFLIGYLRYVFKAN*LRREFRVK*S*LQISLALLAMGVNTFAVVATYLPAAPELYPG
QEAYYVFISIALPMFICTLLLGLFIYLINYA*NKKRGINLSEHSNLTNVDQNVLDVINN*HLYKDQKEFAKFERHVQLLQ
QKQVIVRTTKDKAQRELVANEMQYLAAEIATRFAPEEEH
>Mature_758_residues
GFVLLTASAIIAFYTFPSLSTAG*IAIIFAFITAMC*FIPIGIAAAEMATIKGWAEGGIFTWVRNLLGPRNGFIVT*LQF
QVTFGFVAMILFVLSSFSFAFAGADGYNYFNTLKLGTTLNQATSVNFSKNFSNGALYGIGIVVVAGLIFMSLLGQKRTHQ
FGQVALIIGILLPFFIILGFQIYTVATMPDPLYFIGKHFQKEPHLDPSVTGTSYELNKTFMSSVVMASFMAFSFSLHGVE
VSAIAANRMDNPSKKYPQAMMIVVISALVCIIIGSIMISMTVPTSTLSFNGGLVQTIMFNLSVGGIDVKLPDGTVAHYKT
LAHAYSVIETQYGTAAANQVMYQFVYGHPINTGSDLARSDLLTLTSEVNDPLKNPSLFISVMNIETNKMAMQGLQAITFF
IGIGVFVEVSV*MSNLSTGLNYAMRKAHFPAWVTYRLKNGSALTVAIL*IVLLMCIFAIYMFGYNSLMQYKGQTISDILA
NEIRINQDQKEPMALLSNQNLDEIKLMARSIYEHSHAPGAQANVLHFDFNKHANSEVKAIYDKIKDYIGINAVASGTPAS
ITNISFISNVVSQISMYYIGYNIFLIGYLRYVFKAN*LRREFRVK*S*LQISLALLAMGVNTFAVVATYLPAAPELYPGQ
EAYYVFISIALPMFICTLLLGLFIYLINYA*NKKRGINLSEHSNLTNVDQNVLDVINN*HLYKDQKEFAKFERHVQLLQQ
KQVIVRTTKDKAQRELVANEMQYLAAEIATRFAPEEEH

Specific function: Involved in glutamate-dependent acid resistance. Imports glutamate inside the cell while simultaneously exporting to the periplasm the GABA produced by gadA and gadB. The gad system helps to maintain a near-neutral intracellular pH when cells are exposed

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Glutamate:GABA antiporter (GGA) (TC 2.A.3.7) family [H]

Homologues:

Organism=Escherichia coli, GI1787768, Length=300, Percent_Identity=32, Blast_Score=140, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004759 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 82673; Mature: 82542

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFVLLTASAIIAFYTFPSLSTAGIAIIFAFITAMCFIPIGIAAAEMATIKGWAEGGIFT
CCEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
WVRNLLGPRNGFIVTLQFQVTFGFVAMILFVLSSFSFAFAGADGYNYFNTLKLGTTLNQA
HHHHHCCCCCCEEEEEEEEHHHHHHHHHHHHHHCCCCEEECCCCCCHHHEEEECCCCCHH
TSVNFSKNFSNGALYGIGIVVVAGLIFMSLLGQKRTHQFGQVALIIGILLPFFIILGFQI
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YTVATMPDPLYFIGKHFQKEPHLDPSVTGTSYELNKTFMSSVVMASFMAFSFSLHGVEVS
EEEEECCCCHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHEECCEEEE
AIAANRMDNPSKKYPQAMMIVVISALVCIIIGSIMISMTVPTSTLSFNGGLVQTIMFNLS
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEECCCCHHHHHHHHEE
VGGIDVKLPDGTVAHYKTLAHAYSVIETQYGTAAANQVMYQFVYGHPINTGSDLARSDLL
ECCEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
TLTSEVNDPLKNPSLFISVMNIETNKMAMQGLQAITFFIGIGVFVEVSVMSNLSTGLNYA
HHHHHHCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MRKAHFPAWVTYRLKNGSALTVAILIVLLMCIFAIYMFGYNSLMQYKGQTISDILANEIR
HHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
INQDQKEPMALLSNQNLDEIKLMARSIYEHSHAPGAQANVLHFDFNKHANSEVKAIYDKI
CCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHH
KDYIGINAVASGTPASITNISFISNVVSQISMYYIGYNIFLIGYLRYVFKANLRREFRVK
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLQISLALLAMGVNTFAVVATYLPAAPELYPGQEAYYVFISIALPMFICTLLLGLFIYLI
HHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NYANKKRGINLSEHSNLTNVDQNVLDVINNHLYKDQKEFAKFERHVQLLQQKQVIVRTTK
HHCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEECH
DKAQRELVANEMQYLAAEIATRFAPEEEH
HHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GFVLLTASAIIAFYTFPSLSTAGIAIIFAFITAMCFIPIGIAAAEMATIKGWAEGGIFT
CEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
WVRNLLGPRNGFIVTLQFQVTFGFVAMILFVLSSFSFAFAGADGYNYFNTLKLGTTLNQA
HHHHHCCCCCCEEEEEEEEHHHHHHHHHHHHHHCCCCEEECCCCCCHHHEEEECCCCCHH
TSVNFSKNFSNGALYGIGIVVVAGLIFMSLLGQKRTHQFGQVALIIGILLPFFIILGFQI
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YTVATMPDPLYFIGKHFQKEPHLDPSVTGTSYELNKTFMSSVVMASFMAFSFSLHGVEVS
EEEEECCCCHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHEECCEEEE
AIAANRMDNPSKKYPQAMMIVVISALVCIIIGSIMISMTVPTSTLSFNGGLVQTIMFNLS
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEECCCCHHHHHHHHEE
VGGIDVKLPDGTVAHYKTLAHAYSVIETQYGTAAANQVMYQFVYGHPINTGSDLARSDLL
ECCEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
TLTSEVNDPLKNPSLFISVMNIETNKMAMQGLQAITFFIGIGVFVEVSVMSNLSTGLNYA
HHHHHHCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MRKAHFPAWVTYRLKNGSALTVAILIVLLMCIFAIYMFGYNSLMQYKGQTISDILANEIR
HHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
INQDQKEPMALLSNQNLDEIKLMARSIYEHSHAPGAQANVLHFDFNKHANSEVKAIYDKI
CCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHH
KDYIGINAVASGTPASITNISFISNVVSQISMYYIGYNIFLIGYLRYVFKANLRREFRVK
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLQISLALLAMGVNTFAVVATYLPAAPELYPGQEAYYVFISIALPMFICTLLLGLFIYLI
HHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NYANKKRGINLSEHSNLTNVDQNVLDVINNHLYKDQKEFAKFERHVQLLQQKQVIVRTTK
HHCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEECH
DKAQRELVANEMQYLAAEIATRFAPEEEH
HHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-aminobutyrate [Cytoplasm]; L-glutamate [Periplasm] [C]

Specific reaction: 4-aminobutyrate [Cytoplasm] + L-glutamate [Periplasm] = L-glutamate [Cytoplasm] + 4-aminobutyrate [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]