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The map label for this gene is pfkA

Identifier: 13357743

GI number: 13357743

Start: 223081

End: 224046

Strand: Direct

Name: pfkA

Synonym: UU185

Alternate gene names: 13357743

Gene position: 223081-224046 (Clockwise)

Preceding gene: 13357742

Following gene: 13357744

Centisome position: 29.68

GC content: 24.43

Gene sequence:

>966_bases
ATGAACCAAGTAAATTTTTTAAATTTAGATAAAAATATTTTAATTATTACTTCAGGTGGTGATGCACCGGGTATGAATGC
TTCATTAATTTCATTAATCCATAGATTAATGAATAATAATTTTAATGTTTTTATAGGAATTGAAGGATTATTAGGACTGT
ACAATAATTTAATTGAACCAATTAAAGATAAACGTGTTTTTGATGTTTATTTTAACGAGCAAGGAACAGTCATTAAGACA
AGTAGATTTATTAAATTAGATATTAATGATAAAAAAACACAAATTATTAAAGATAATTTATTAAGTCATAATATTCAAAA
AATTATTATCTTAGGCGGCCAAGGTAGTATGCAAGCTGGTTTAGTGTTAACAAAAATGGGCTTTGAAGTCTTTGGAATAT
TACACACTATTGATAATGATTTTAGTGAAACACAAATGTGTATAGGGGCCTTAAGTGCTGCTTCTTTTAATCAAAAATTA
TTAAAGTGCCTAAATTACACAGCAAAAGCTCATTGTGCTTTTAATTTAGTTGAGCTTATGGGTCGTGAATGTTCATGATT
AGTCAATAATAGTGTTGGTAAATTAAAACCTATTTTAATGTTAACTAATCAAGATAATAAATATACTGTTGATGAAGTAA
TAGATTTAATTAAAGATAAAATAAACTCAATAAAAGAATACGATCCTTTAATCATTGTTCAAGAATTAATCTATGATCAA
AAATGATACGAATTATTAGTAAAAACTTTTGAGCAAAAATTACATAAAAGTTTACGAATTACAATTTTAAATTATTTACA
ACGCGGAGCTCCAGTAAGTGATTTTGATTTACAATTAGCAAAAGATAGTGCGAATGTATTGGTTGATTTTATTGTTAATC
AAAATGATATAAAAAACACAAATAATATGTATGTAGTTATTAATAAGTTTGATAATAAGTTAGAAGTTATTAAATTCAAT
AAATAG

Upstream 100 bases:

>100_bases
GCTGGGCAAATTAAAACAGGTTCATTATCACGTTCTGAACGGATAGCGAAGTATAATCGTATTTTAGAAATCGAACAAGA
ATTAAAGGATAAATTAATTT

Downstream 100 bases:

>100_bases
AGGGAAAATTAATGTTTTCAAAAACACGCATTATTGCTACAGTAGGTCCAGCAACAACAAAATTGTTTTCTAATTGGTTA
GAATTTAACGATTTAAATAA

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MNQVNFLNLDKNILIITSGGDAPGMNASLISLIHRLMNNNFNVFIGIEGLLGLYNNLIEPIKDKRVFDVYFNEQGTVIKT
SRFIKLDINDKKTQIIKDNLLSHNIQKIIILGGQGSMQAGLVLTKMGFEVFGILHTIDNDFSETQMCIGALSAASFNQKL
LKCLNYTAKAHCAFNLVELMGRECSWLVNNSVGKLKPILMLTNQDNKYTVDEVIDLIKDKINSIKEYDPLIIVQELIYDQ
KWYELLVKTFEQKLHKSLRITILNYLQRGAPVSDFDLQLAKDSANVLVDFIVNQNDIKNTNNMYVVINKFDNKLEVIKFN
K

Sequences:

>Translated_321_residues
MNQVNFLNLDKNILIITSGGDAPGMNASLISLIHRLMNNNFNVFIGIEGLLGLYNNLIEPIKDKRVFDVYFNEQGTVIKT
SRFIKLDINDKKTQIIKDNLLSHNIQKIIILGGQGSMQAGLVLTKMGFEVFGILHTIDNDFSETQMCIGALSAASFNQKL
LKCLNYTAKAHCAFNLVELMGRECS*LVNNSVGKLKPILMLTNQDNKYTVDEVIDLIKDKINSIKEYDPLIIVQELIYDQ
K*YELLVKTFEQKLHKSLRITILNYLQRGAPVSDFDLQLAKDSANVLVDFIVNQNDIKNTNNMYVVINKFDNKLEVIKFN
K
>Mature_321_residues
MNQVNFLNLDKNILIITSGGDAPGMNASLISLIHRLMNNNFNVFIGIEGLLGLYNNLIEPIKDKRVFDVYFNEQGTVIKT
SRFIKLDINDKKTQIIKDNLLSHNIQKIIILGGQGSMQAGLVLTKMGFEVFGILHTIDNDFSETQMCIGALSAASFNQKL
LKCLNYTAKAHCAFNLVELMGRECS*LVNNSVGKLKPILMLTNQDNKYTVDEVIDLIKDKINSIKEYDPLIIVQELIYDQ
K*YELLVKTFEQKLHKSLRITILNYLQRGAPVSDFDLQLAKDSANVLVDFIVNQNDIKNTNNMYVVINKFDNKLEVIKFN
K

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI48762920, Length=303, Percent_Identity=25.0825082508251, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI1790350, Length=281, Percent_Identity=27.7580071174377, Blast_Score=118, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17558788, Length=307, Percent_Identity=26.0586319218241, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321679, Length=325, Percent_Identity=26.7692307692308, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323861, Length=301, Percent_Identity=25.2491694352159, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28573326, Length=308, Percent_Identity=25.6493506493506, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR022953
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 36134; Mature: 36134

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQVNFLNLDKNILIITSGGDAPGMNASLISLIHRLMNNNFNVFIGIEGLLGLYNNLIEP
CCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHH
IKDKRVFDVYFNEQGTVIKTSRFIKLDINDKKTQIIKDNLLSHNIQKIIILGGQGSMQAG
HCCCEEEEEEECCCCCEEEECEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCC
LVLTKMGFEVFGILHTIDNDFSETQMCIGALSAASFNQKLLKCLNYTAKAHCAFNLVELM
EEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHC
GRECSLVNNSVGKLKPILMLTNQDNKYTVDEVIDLIKDKINSIKEYDPLIIVQELIYDQK
CCCHHHHCCCCCCEEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCH
YELLVKTFEQKLHKSLRITILNYLQRGAPVSDFDLQLAKDSANVLVDFIVNQNDIKNTNN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCC
MYVVINKFDNKLEVIKFNK
EEEEEEECCCEEEEEEECC
>Mature Secondary Structure
MNQVNFLNLDKNILIITSGGDAPGMNASLISLIHRLMNNNFNVFIGIEGLLGLYNNLIEP
CCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHH
IKDKRVFDVYFNEQGTVIKTSRFIKLDINDKKTQIIKDNLLSHNIQKIIILGGQGSMQAG
HCCCEEEEEEECCCCCEEEECEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCC
LVLTKMGFEVFGILHTIDNDFSETQMCIGALSAASFNQKLLKCLNYTAKAHCAFNLVELM
EEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHC
GRECSLVNNSVGKLKPILMLTNQDNKYTVDEVIDLIKDKINSIKEYDPLIIVQELIYDQK
CCCHHHHCCCCCCEEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCH
YELLVKTFEQKLHKSLRITILNYLQRGAPVSDFDLQLAKDSANVLVDFIVNQNDIKNTNN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCC
MYVVINKFDNKLEVIKFNK
EEEEEEECCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11048724 [H]