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The map label for this gene is potB

Identifier: 13357665

GI number: 13357665

Start: 138334

End: 139341

Strand: Direct

Name: potB

Synonym: UU108

Alternate gene names: 13357665

Gene position: 138334-139341 (Clockwise)

Preceding gene: 13357664

Following gene: 13357666

Centisome position: 18.4

GC content: 25.69

Gene sequence:

>1008_bases
ATGAACAAGCAATTTAATTTCAATTTTGCACCATCTAAATTATCAGATATTAACAAAAAAAACGAAACAGTATTTTTTTT
AAAAGAAGTTAAAGTAATTAAAAAAAACAATCGTAAGCATTATAAACAAACTCTTTTTGATAAATTAAAAATGAATAAGG
CACTAATATTAACAATTCCTTATTTTTTAGTAACTATTTTTTTATTGATAATACCATTGGTTAGTATTATTGTTAAAACT
TTTATTAACAATACTGATTCAACTGGTGTAACAACAAACATCATTGATAATTGGTCAGTAATTACGCCAACTATTGGAAT
GAAAATTTTTAATTCGTTATGAATCGCTGCAGTTGCTACAATTTTTTGTTTAATTATTGGTTATCCATTTGCGTATTTTT
TAAGTATTGGTAAATCAAAATTTTTAAAAGCAATTGCAATTGCACTAATTACTTCACCAATTTGGATGAGTATGTTAGTA
AAACTAATTGGTATTAAAACCTTTTTTGATGTAGTAAATGGAGAAATTAATAGTACACATGGGCACATTTTTACTATTTT
AGGTTTAGTTTATATTAATTTGCCATTAATGATTTTGCCTATTTATACATCTTTGTCAACAATGCCTAAAAACCTAGTTA
ATGCTAGTTATGATTTAGGAAGAGGTTTTTTTTACACCTTCTTTCATATTGTAATTCCTTACACTAAAAATGCTTTAATT
TCAGGAGTTTGGATTGTTTATTTAGCAGCATTTACATCAGTTGTGGTTTCAGGTTTTTTAAATAATGATAACGCTGGAGG
ACTAATTGGTGGAGAAATCTTTGCTCAAGGACAAGACGGTATTTCAAGTGGAGTTCAACTATCACGGTCATCAACAATTA
CTTTAGTAATTAGTTTAATTTTATTAGGGATTTATCTTATTATTGTTATTATTCCAAAAATAGTCCAATTAATTTATTAC
AAACGAAAAAATCTAAAAAAAGGAGTAAAGAATAATGAAGCAGATTAA

Upstream 100 bases:

>100_bases
ATTAGTGTTGAAGGTGTTAATAAAGTTAATGAAGGTGATGAAATTGGTTTAGATTGAGATGACATTGATGTGCATGTAAT
TAAAAAGGATTATTTAAATA

Downstream 100 bases:

>100_bases
TTGATTGTCTTGATTGCGTAATTTTTATATTTGAATTATTTTAGCAATCATTTATATTCCTTTAATTATCATTGTTTTAT
TATCATTTACCACACCTTCA

Product: spermidine/putrescine transport system permease

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MNKQFNFNFAPSKLSDINKKNETVFFLKEVKVIKKNNRKHYKQTLFDKLKMNKALILTIPYFLVTIFLLIIPLVSIIVKT
FINNTDSTGVTTNIIDNWSVITPTIGMKIFNSLWIAAVATIFCLIIGYPFAYFLSIGKSKFLKAIAIALITSPIWMSMLV
KLIGIKTFFDVVNGEINSTHGHIFTILGLVYINLPLMILPIYTSLSTMPKNLVNASYDLGRGFFYTFFHIVIPYTKNALI
SGVWIVYLAAFTSVVVSGFLNNDNAGGLIGGEIFAQGQDGISSGVQLSRSSTITLVISLILLGIYLIIVIIPKIVQLIYY
KRKNLKKGVKNNEAD

Sequences:

>Translated_335_residues
MNKQFNFNFAPSKLSDINKKNETVFFLKEVKVIKKNNRKHYKQTLFDKLKMNKALILTIPYFLVTIFLLIIPLVSIIVKT
FINNTDSTGVTTNIIDNWSVITPTIGMKIFNSL*IAAVATIFCLIIGYPFAYFLSIGKSKFLKAIAIALITSPIWMSMLV
KLIGIKTFFDVVNGEINSTHGHIFTILGLVYINLPLMILPIYTSLSTMPKNLVNASYDLGRGFFYTFFHIVIPYTKNALI
SGVWIVYLAAFTSVVVSGFLNNDNAGGLIGGEIFAQGQDGISSGVQLSRSSTITLVISLILLGIYLIIVIIPKIVQLIYY
KRKNLKKGVKNNEAD
>Mature_335_residues
MNKQFNFNFAPSKLSDINKKNETVFFLKEVKVIKKNNRKHYKQTLFDKLKMNKALILTIPYFLVTIFLLIIPLVSIIVKT
FINNTDSTGVTTNIIDNWSVITPTIGMKIFNSL*IAAVATIFCLIIGYPFAYFLSIGKSKFLKAIAIALITSPIWMSMLV
KLIGIKTFFDVVNGEINSTHGHIFTILGLVYINLPLMILPIYTSLSTMPKNLVNASYDLGRGFFYTFFHIVIPYTKNALI
SGVWIVYLAAFTSVVVSGFLNNDNAGGLIGGEIFAQGQDGISSGVQLSRSSTITLVISLILLGIYLIIVIIPKIVQLIYY
KRKNLKKGVKNNEAD

Specific function: Required for the activity of the bacterial transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=251, Percent_Identity=32.6693227091633, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1787080, Length=187, Percent_Identity=29.4117647058824, Blast_Score=84, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 37241; Mature: 37241

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKQFNFNFAPSKLSDINKKNETVFFLKEVKVIKKNNRKHYKQTLFDKLKMNKALILTIP
CCCCCCCCCCCHHHHHCCCCCCEEEHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEHHH
YFLVTIFLLIIPLVSIIVKTFINNTDSTGVTTNIIDNWSVITPTIGMKIFNSLIAAVATI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEECHHHHHHHHHHHHHHHHHH
FCLIIGYPFAYFLSIGKSKFLKAIAIALITSPIWMSMLVKLIGIKTFFDVVNGEINSTHG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
HIFTILGLVYINLPLMILPIYTSLSTMPKNLVNASYDLGRGFFYTFFHIVIPYTKNALIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHH
GVWIVYLAAFTSVVVSGFLNNDNAGGLIGGEIFAQGQDGISSGVQLSRSSTITLVISLIL
HHHHHHHHHHHHHHHHHHCCCCCCCCEECCHHHCCCCCCHHHCCEECCCHHHHHHHHHHH
LGIYLIIVIIPKIVQLIYYKRKNLKKGVKNNEAD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNKQFNFNFAPSKLSDINKKNETVFFLKEVKVIKKNNRKHYKQTLFDKLKMNKALILTIP
CCCCCCCCCCCHHHHHCCCCCCEEEHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEHHH
YFLVTIFLLIIPLVSIIVKTFINNTDSTGVTTNIIDNWSVITPTIGMKIFNSLIAAVATI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEECHHHHHHHHHHHHHHHHHH
FCLIIGYPFAYFLSIGKSKFLKAIAIALITSPIWMSMLVKLIGIKTFFDVVNGEINSTHG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
HIFTILGLVYINLPLMILPIYTSLSTMPKNLVNASYDLGRGFFYTFFHIVIPYTKNALIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHH
GVWIVYLAAFTSVVVSGFLNNDNAGGLIGGEIFAQGQDGISSGVQLSRSSTITLVISLIL
HHHHHHHHHHHHHHHHHHCCCCCCCCEECCHHHCCCCCCHHHCCEECCCHHHHHHHHHHH
LGIYLIIVIIPKIVQLIYYKRKNLKKGVKNNEAD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]