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Identifier: 13357633

GI number: 13357633

Start: 93842

End: 94843

Strand: Reverse

Name: Not Available

Synonym: UU077

Alternate gene names: 13357633

Gene position: 94843-93842 (Counterclockwise)

Preceding gene: 13357634

Following gene: 13357632

Centisome position: 12.62

GC content: 24.25

Gene sequence:

>1002_bases
GTGAAGATTTTGATAAAGTTATGAACAAATTTAATTAATAGAATTAGTAATAAAAAATACTTAGCTGCTGTTTCTGGTGG
TCCAGATTCTATGGCAATGTTAAATATGTACAAAAGAAATATTCGTGTTGTTTGTCATGTGAATTATCATAAACGCGAAT
CATCCGATCGTGATCAGCAAATAGTTGTCGATTTTTGTAGAAAAAATAATTTAATAATTGAAATTTTAGATGTTGATGAA
AAAGTTTATGAACGTTATGGTCATATTGATAATTTTCAAACAAAAGCACGTTTAATTCGTTATGATTTTTTCAAAGAAAT
TGGTAAAAAATATAATATTGAAAATTTGTATATAGCGCATAATTTTGATGATTTTTTAGAAACGGCATATATGCAACGAG
CTCGCCAATCAAAAGCCTTATTTTATGGCATTAAGGAAAGTAATGTTATTAATGGCATAATTGTTAAACGTCCGGTTTTA
TTTGTGCGTAAACAAACATTACAACGCTATTGTGATGAAAATAAAATTAAGTATGGTATTGACGAAACAAATGAATTAGA
TATATACGAACGCAATCGTGTTCGTAAAATAATTAGTTCTTGAAGTTTAAATGAGGTTTATGATTTTAAAAAATTCGTTA
TTAAATATAATAAAGAACATAGTTCTTTTGCTAATTTTATAGATTTATCATATATTGAATTTAAAAAAAATAAATACAAA
TATGACTACTTTATTCGTCAAGACGATATGGTTCAATATTATTTAATTTACTACTTTTTAATTGATCATAAAATTATTAA
TCCAAGTGAAAATAAAATCATTTCATTAATCAAATTCTTTGGTAAACAAATTAATAAAGAAAAAGCTTATCGTGTTCAAG
AAAATATTTATATGCATGTTAATGATGATGAATTAATTAGTTTAATATCATACAATAAAAGCGATAATATTAGCAACTCT
AATGTTGATATTATCGATGATCATAATGTTATTGAGAAATAG

Upstream 100 bases:

>100_bases
AAATTAGATCAATATGTCTTATCGAATTTTAAGATTGACGAATTAGTTATAATTAAACCAGCTATTATAAAGGGCGCTTT
AGCTACATTAGCAGCGATTG

Downstream 100 bases:

>100_bases
GTAGATAATAATTAATGGAAATTCGTGGTAAATTATTTGTTAGTCAAGTTGTTAGAAAGTTTAATTTAAACGTTGTTGCA
AATTCTGACTATATTGATCG

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MKILIKLWTNLINRISNKKYLAAVSGGPDSMAMLNMYKRNIRVVCHVNYHKRESSDRDQQIVVDFCRKNNLIIEILDVDE
KVYERYGHIDNFQTKARLIRYDFFKEIGKKYNIENLYIAHNFDDFLETAYMQRARQSKALFYGIKESNVINGIIVKRPVL
FVRKQTLQRYCDENKIKYGIDETNELDIYERNRVRKIISSWSLNEVYDFKKFVIKYNKEHSSFANFIDLSYIEFKKNKYK
YDYFIRQDDMVQYYLIYYFLIDHKIINPSENKIISLIKFFGKQINKEKAYRVQENIYMHVNDDELISLISYNKSDNISNS
NVDIIDDHNVIEK

Sequences:

>Translated_333_residues
MKILIKL*TNLINRISNKKYLAAVSGGPDSMAMLNMYKRNIRVVCHVNYHKRESSDRDQQIVVDFCRKNNLIIEILDVDE
KVYERYGHIDNFQTKARLIRYDFFKEIGKKYNIENLYIAHNFDDFLETAYMQRARQSKALFYGIKESNVINGIIVKRPVL
FVRKQTLQRYCDENKIKYGIDETNELDIYERNRVRKIISS*SLNEVYDFKKFVIKYNKEHSSFANFIDLSYIEFKKNKYK
YDYFIRQDDMVQYYLIYYFLIDHKIINPSENKIISLIKFFGKQINKEKAYRVQENIYMHVNDDELISLISYNKSDNISNS
NVDIIDDHNVIEK
>Mature_333_residues
MKILIKL*TNLINRISNKKYLAAVSGGPDSMAMLNMYKRNIRVVCHVNYHKRESSDRDQQIVVDFCRKNNLIIEILDVDE
KVYERYGHIDNFQTKARLIRYDFFKEIGKKYNIENLYIAHNFDDFLETAYMQRARQSKALFYGIKESNVINGIIVKRPVL
FVRKQTLQRYCDENKIKYGIDETNELDIYERNRVRKIISS*SLNEVYDFKKFVIKYNKEHSSFANFIDLSYIEFKKNKYK
YDYFIRQDDMVQYYLIYYFLIDHKIINPSENKIISLIKFFGKQINKEKAYRVQENIYMHVNDDELISLISYNKSDNISNS
NVDIIDDHNVIEK

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 39729; Mature: 39729

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILIKLTNLINRISNKKYLAAVSGGPDSMAMLNMYKRNIRVVCHVNYHKRESSDRDQQI
CEEEEEHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCEEEEEEEECCCCCCCCCHHHH
VVDFCRKNNLIIEILDVDEKVYERYGHIDNFQTKARLIRYDFFKEIGKKYNIENLYIAHN
HHHHHCCCCEEEEEECCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
FDDFLETAYMQRARQSKALFYGIKESNVINGIIVKRPVLFVRKQTLQRYCDENKIKYGID
HHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCEEECCC
ETNELDIYERNRVRKIISSSLNEVYDFKKFVIKYNKEHSSFANFIDLSYIEFKKNKYKYD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEECCCEEEE
YFIRQDDMVQYYLIYYFLIDHKIINPSENKIISLIKFFGKQINKEKAYRVQENIYMHVND
EEECCCCHHHHHHHHHHHHHCEEECCCHHHHHHHHHHHHHHCCHHHHHEEECCEEEEECH
DELISLISYNKSDNISNSNVDIIDDHNVIEK
HHHHHHHHCCCCCCCCCCCCEEEECCCCCCC
>Mature Secondary Structure
MKILIKLTNLINRISNKKYLAAVSGGPDSMAMLNMYKRNIRVVCHVNYHKRESSDRDQQI
CEEEEEHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCEEEEEEEECCCCCCCCCHHHH
VVDFCRKNNLIIEILDVDEKVYERYGHIDNFQTKARLIRYDFFKEIGKKYNIENLYIAHN
HHHHHCCCCEEEEEECCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
FDDFLETAYMQRARQSKALFYGIKESNVINGIIVKRPVLFVRKQTLQRYCDENKIKYGID
HHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCEEECCC
ETNELDIYERNRVRKIISSSLNEVYDFKKFVIKYNKEHSSFANFIDLSYIEFKKNKYKYD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEECCCEEEE
YFIRQDDMVQYYLIYYFLIDHKIINPSENKIISLIKFFGKQINKEKAYRVQENIYMHVND
EEECCCCHHHHHHHHHHHHHCEEECCCHHHHHHHHHHHHHHCCHHHHHEEECCEEEEECH
DELISLISYNKSDNISNSNVDIIDDHNVIEK
HHHHHHHHCCCCCCCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11048724 [H]