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The map label for this gene is dnaH

Identifier: 13357575

GI number: 13357575

Start: 19673

End: 20467

Strand: Reverse

Name: dnaH

Synonym: UU019

Alternate gene names: 13357575

Gene position: 20467-19673 (Counterclockwise)

Preceding gene: 13357576

Following gene: 13357574

Centisome position: 2.72

GC content: 20.0

Gene sequence:

>795_bases
ATGAAATACAGTTTTGCTAATTTATTAATTCAATCACCTAAAACCTCACTAGAATCAGGTATTGAAGAAATTATGTTAGC
TTTTATTTATGAGAAAAATCATAAAGAACAATCATTTTATGTTACTAAAGTTAAAAATAAACAATACTTTGATTTAAAAA
TTTATGATGGACTATCAATGAAAAAAAGCGATGTAATTGATTTACAAAACACTTTTTTATGTGATGGAGTAGAAGATATT
AATTTAAAATTTTATCTTATTAAAAATATTGACTTAGCATCAAAATATGTTTTAAATGCATTATTAAAATTTATTGAAGA
ACCGCCAAAAAATACAATTGCAATTTTTAGCACTAAAAATTTAAATCAAGTTTTAAAAACTATTAAAAGTCGTTGTCAGC
TTTTTTATTTACCTACTAATCATGATTTATATAAAAAATTAATTAACCAAATTAATCAACCAATTGATCTCGTGGAATGC
GACTTAATGTTTGATGATTTGGATGAGCTAAAAATTTTATTAGAAAACAAGGAAATCAATGATGTTTTAACTTATTATGG
AAAATTAAGTGATTTAAGAAGTTTTGAAATATTAAATGATTTAAAAGAAATATTTAAAAATTTATCTACCTTACAAATTC
ATTATCTATTAAAATTAATCTTCATTAGAATTAATAATGTAAATAGCAAAGAAATTATTTTGGATTTAATGCGTGCGAAT
TTAAAAATTAATATTAATAAAAATAATTTGTTTACTATTATTTATACAATTATTAATAAAAATAAAGGTGATTAA

Upstream 100 bases:

>100_bases
AAAAATTATTTTTATTGATGCCAATAAAAATGAAAATGAAGTATTATGTGAGGTTAAAGAACAATTATTAAAAATTTTTA
AAGAACATAAATATATATAA

Downstream 100 bases:

>100_bases
CAATGTCAACAATTGTTGCTTTAGCAACCGCGCCTATGAATTGTGCCATTCACATTATTCGGGTTAGTGGTCCAAAAGCT
TTTGAAATAATTAATAAAAT

Product: DNA polymerase III delta subunit

Products: diphosphate; DNAn+1

Alternate protein names: DNA Polymerase III Subunit Delta; DNA Polymerase III Delta Prime Subunit

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKYSFANLLIQSPKTSLESGIEEIMLAFIYEKNHKEQSFYVTKVKNKQYFDLKIYDGLSMKKSDVIDLQNTFLCDGVEDI
NLKFYLIKNIDLASKYVLNALLKFIEEPPKNTIAIFSTKNLNQVLKTIKSRCQLFYLPTNHDLYKKLINQINQPIDLVEC
DLMFDDLDELKILLENKEINDVLTYYGKLSDLRSFEILNDLKEIFKNLSTLQIHYLLKLIFIRINNVNSKEIILDLMRAN
LKININKNNLFTIIYTIINKNKGD

Sequences:

>Translated_264_residues
MKYSFANLLIQSPKTSLESGIEEIMLAFIYEKNHKEQSFYVTKVKNKQYFDLKIYDGLSMKKSDVIDLQNTFLCDGVEDI
NLKFYLIKNIDLASKYVLNALLKFIEEPPKNTIAIFSTKNLNQVLKTIKSRCQLFYLPTNHDLYKKLINQINQPIDLVEC
DLMFDDLDELKILLENKEINDVLTYYGKLSDLRSFEILNDLKEIFKNLSTLQIHYLLKLIFIRINNVNSKEIILDLMRAN
LKININKNNLFTIIYTIINKNKGD
>Mature_264_residues
MKYSFANLLIQSPKTSLESGIEEIMLAFIYEKNHKEQSFYVTKVKNKQYFDLKIYDGLSMKKSDVIDLQNTFLCDGVEDI
NLKFYLIKNIDLASKYVLNALLKFIEEPPKNTIAIFSTKNLNQVLKTIKSRCQLFYLPTNHDLYKKLINQINQPIDLVEC
DLMFDDLDELKILLENKEINDVLTYYGKLSDLRSFEILNDLKEIFKNLSTLQIHYLLKLIFIRINNVNSKEIILDLMRAN
LKININKNNLFTIIYTIINKNKGD

Specific function: DNA Polymerase III Is A Complex, Multichain Enzyme Responsible For Most Of The Replicative Synthesis In Bacteria. This DNA Polymerase Also Exhibits 3' To 5' Exonuclease Activity. The Tau Chain Serves As A Scaffold To Help In The Dimerizaton Of The Core Co

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.7.7

Molecular weight: Translated: 30986; Mature: 30986

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYSFANLLIQSPKTSLESGIEEIMLAFIYEKNHKEQSFYVTKVKNKQYFDLKIYDGLSM
CCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEEEEECCCCC
KKSDVIDLQNTFLCDGVEDINLKFYLIKNIDLASKYVLNALLKFIEEPPKNTIAIFSTKN
CCCCCCCCCCCEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEECCC
LNQVLKTIKSRCQLFYLPTNHDLYKKLINQINQPIDLVECDLMFDDLDELKILLENKEIN
HHHHHHHHHHCCEEEEECCCCHHHHHHHHHCCCCCCEEEHHHHHCCHHHHHHHHCCCCHH
DVLTYYGKLSDLRSFEILNDLKEIFKNLSTLQIHYLLKLIFIRINNVNSKEIILDLMRAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
LKININKNNLFTIIYTIINKNKGD
EEEEECCCCEEEEEEEEECCCCCC
>Mature Secondary Structure
MKYSFANLLIQSPKTSLESGIEEIMLAFIYEKNHKEQSFYVTKVKNKQYFDLKIYDGLSM
CCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEEEEECCCCC
KKSDVIDLQNTFLCDGVEDINLKFYLIKNIDLASKYVLNALLKFIEEPPKNTIAIFSTKN
CCCCCCCCCCCEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEECCC
LNQVLKTIKSRCQLFYLPTNHDLYKKLINQINQPIDLVECDLMFDDLDELKILLENKEIN
HHHHHHHHHHCCEEEEECCCCHHHHHHHHHCCCCCCEEEHHHHHCCHHHHHHHHCCCCHH
DVLTYYGKLSDLRSFEILNDLKEIFKNLSTLQIHYLLKLIFIRINNVNSKEIILDLMRAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
LKININKNNLFTIIYTIINKNKGD
EEEEECCCCEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: deoxynucleoside triphosphate; DNAn

Specific reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA