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The map label for this gene is ctfB [H]

Identifier: 126700292

GI number: 126700292

Start: 3093907

End: 3094572

Strand: Direct

Name: ctfB [H]

Synonym: CD2678

Alternate gene names: 126700292

Gene position: 3093907-3094572 (Clockwise)

Preceding gene: 126700291

Following gene: 126700293

Centisome position: 72.11

GC content: 33.63

Gene sequence:

>666_bases
ATGGATAAATTAGAAATGCAAGAATATATTGCAAATAGAGTATCTAAAGAATTAAAAGACGGAGCCGTTGTAAACCTTGG
AATAGGTCTACCAACCAAAGTAGCAAATTATATTCCGGATAATGTAAACGTTATCTTACAATCTGAAAATGGCTTTTTAG
GTTTAGGTTCAGCCGAAGATGGAAATTCAAGTGATGAAACTATTGTAAATGCAGGTGGTCAACCAGTTACTATACTTCCT
GGAGGATGTTTCTTTGATAGTGCTACTTCTTTTGGAATAATTAGAGGAGGTCATGTTGATATAACTGTTCTTGGTGCACT
TCAGGTTGACAAATATGGCAATATAGCAAACTATATGATTCCAGGTAAGATGGTTCCAGGTATGGGTGGAGCTATGGACC
TAGTAACTGGAGCTAAAAAAGTTATTGTAGCAATGGAACATACTTCAAAAGGTTCTGCAAAAATATTAAATAATTGTACT
CTTCCACTTACTGCAACAAATGCTGTAGATTTGATAGTTACTGAAATGGGAGTTATGGAAGTAACATCAGATGGAATTTT
ACTTAAAGAAATAAATCCAGCTTTCACATTAGATGATGTTATTTCTGCTACTGAAGCTCCTTTAATATTGTCAGATTCAT
TAAATGGAAAAATATCTGTTGTTTAA

Upstream 100 bases:

>100_bases
TTGAATCAGATGAAATCGTTGAAATTGGTGATATTGACCCAAATGAAGTTAATACACCAGGTATTTTTGTGAATTACATC
GTTGAAGGAGGTAATTTATA

Downstream 100 bases:

>100_bases
AATAATTAAAACTAGAAAATAAAAATCTCTTAGACGTATTACATATAAATTTGTATTGCTTAAAATATTAATATAAAAAT
AAAATAAAATTGGAGGAAAT

Product: butyrate--acetoacetate CoA-transferase subunit B

Products: NA

Alternate protein names: Coat B; Acetoacetyl-CoA:acetate/butyrate:CoA transferase subunit B [H]

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNVILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILP
GGCFFDSATSFGIIRGGHVDITVLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKISVV

Sequences:

>Translated_221_residues
MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNVILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILP
GGCFFDSATSFGIIRGGHVDITVLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKISVV
>Mature_221_residues
MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNVILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILP
GGCFFDSATSFGIIRGGHVDITVLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKISVV

Specific function: Involved in solventogenic switch which allows C.acetobutylicum to uptake acid and produce solvents. Acts mainly to detoxify the medium by removing the acetate and butyrate excreted earlier in the fermentation [H]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI4557817, Length=213, Percent_Identity=51.6431924882629, Blast_Score=212, Evalue=3e-55,
Organism=Homo sapiens, GI11545841, Length=214, Percent_Identity=46.7289719626168, Blast_Score=196, Evalue=1e-50,
Organism=Escherichia coli, GI1788552, Length=213, Percent_Identity=55.3990610328638, Blast_Score=241, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI17531517, Length=212, Percent_Identity=47.6415094339623, Blast_Score=193, Evalue=5e-50,
Organism=Drosophila melanogaster, GI21358325, Length=220, Percent_Identity=49.0909090909091, Blast_Score=206, Evalue=8e-54,
Organism=Drosophila melanogaster, GI24655794, Length=220, Percent_Identity=49.0909090909091, Blast_Score=206, Evalue=9e-54,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.9 [H]

Molecular weight: Translated: 23025; Mature: 23025

Theoretical pI: Translated: 4.25; Mature: 4.25

Prosite motif: PS01274 COA_TRANSF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNVILQSENGFLGLGSAED
CCCHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCCCEEEEEECCCCEEEECCCCC
GNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDITVLGALQVDKYGNIANYMI
CCCCCCEEEECCCCEEEEECCCCEECCCCCCEEEECCEEEEEEEEEEEECCCCCEEEEEE
PGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCTLPLTATNAVDLIVTEMGVME
CCCCCCCCCCHHHHHCCCEEEEEEEECCCCCCEEECCCCCCEEEECCCEEEEEECCCEEE
VTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKISVV
ECCCCEEEEECCCCEEHHHHHHCCCCCEEEECCCCCEEEEC
>Mature Secondary Structure
MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNVILQSENGFLGLGSAED
CCCHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCCCEEEEEECCCCEEEECCCCC
GNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDITVLGALQVDKYGNIANYMI
CCCCCCEEEECCCCEEEEECCCCEECCCCCCEEEECCEEEEEEEEEEEECCCCCEEEEEE
PGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCTLPLTATNAVDLIVTEMGVME
CCCCCCCCCCHHHHHCCCEEEEEEEECCCCCCEEECCCCCCEEEECCCEEEEEECCCEEE
VTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKISVV
ECCCCEEEEECCCCEEHHHHHHCCCCCEEEECCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2254264; 8226639; 8423010; 11466286; 2383002 [H]