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The map label for this gene is dacF [H]

Identifier: 126700114

GI number: 126700114

Start: 2882690

End: 2883979

Strand: Direct

Name: dacF [H]

Synonym: CD2498

Alternate gene names: 126700114

Gene position: 2882690-2883979 (Clockwise)

Preceding gene: 126700112

Following gene: 126700118

Centisome position: 67.19

GC content: 28.29

Gene sequence:

>1290_bases
ATGAAAAGAAATTTATCACTTTTATTAATCTGTTTATTGATATTTACTTCATTTTTAGGAAGCTCTAATATCTCTTTTGC
AGATAATGAACCAGCTATAGTTGCTAAACATGCAGTTTTAATGGACTATGAGTCTGGCAAAATACTATATAATAAGGATG
GAAATTCTAAGCTTTATCCAGCTTCAACAACTAAAGTATGGACAGCTTGTTTAGTTCTAAAGGAGGTTAAAGATTTAAAT
CAAGTAATAGAAATTAAAGATTTACCTCAAATAGATGGCTCTAGTATGTATCTAAAAAATGGGGAATCTTTTACTGTAAA
GCAGTTACTAGATGCTCTTTTGGTTCATTCTGCTAATGATGCTGCTTTTGTATTAGCTAGATATGTTGGTGGTGGCAATG
TACAAAAATTTATTGACCTAATGAACTCTGAGGCAAAAAAAATTGGAGCTACTAATACTCATTTTAATAATCCTCATGGA
TTACCAGACCCAAATCACTACACAACAGCTCATGATATGGCTCTCATAGCTAGAGAAGCTATGAATAATGATACTTTTAG
ACAAATTGTAAAAACTAAGTCACTTAAATTTGAAGCTACAAAAGCATATCCTTATGAGAGATACTTTGTAAACACTAATA
AGTTTTTGACATCACATGATAAGATTACATACAAAGGTCAACCTATAAATATAAAATATGATATAGTTGATGGTATAAAA
ACTGGATATACTGATGCTGCTGGTAAATGTCTTTTATCGAGTGCTGTAAAAGATGGCAGAAGAGTTATCGTTGCTGTATT
TAATTCAACTAATGCTGATTTATATTTAGACTCACGTATTCTGATTGATTATGGATTTGATAACTTTAAGTGTGCTACTA
TTGTTGATAAAGAAAAATACACAGATACTAAAAAAGTATTGTTTACAAAACAACATGAATTGATTTATGAACCAAAAAAT
AGTTATAGGATATTCTTAGAAAAAGATGAATCTCAAGGCAATTATGATACCAAAACAGAGTTAAATAAAATTGATTTGCC
TATAAAAAAAGGAGCTAAGGTTGGTACTTTAAATGTATATAATAATGGTAAACTAGAAAACAGTATAGACTTAATTGCAA
AAAATAATCTTGATAGCAGTCTTCCATTTTTGACTGAAAATAATGTTTTAATGACTTTTGTTAAGATTATTGCAGGTATT
CTAGTGCTTTTAGTTTTATTTATAATAGCTTCAAATATAAAGAAGAAAAAAAGAAAAAAAGCTAGAAGTAAAAAAAATAT
GAAAAAGTAA

Upstream 100 bases:

>100_bases
AAATTTTACCTTTTTTTTCATATATTAATCACTCCTTTCTTTTAATTATTGACAAATATATGCTATACTAAAAGATGAAT
TTAAAATTTGGAGGCTCACA

Downstream 100 bases:

>100_bases
CAAGTAGTTAGAAATTATAATAAAAAATTTATAATATACTTATCCTTATAAATCTAAAAGTACTGAGATAATAATTATAA
CTATTATCTCAGTACTTTTA

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYESGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLN
QVIEIKDLPQIDGSSMYLKNGESFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTSHDKITYKGQPINIKYDIVDGIK
TGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYLDSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKN
SYRIFLEKDESQGNYDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFLTENNVLMTFVKIIAGI
LVLLVLFIIASNIKKKKRKKARSKKNMKK

Sequences:

>Translated_429_residues
MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYESGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLN
QVIEIKDLPQIDGSSMYLKNGESFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTSHDKITYKGQPINIKYDIVDGIK
TGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYLDSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKN
SYRIFLEKDESQGNYDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFLTENNVLMTFVKIIAGI
LVLLVLFIIASNIKKKKRKKARSKKNMKK
>Mature_429_residues
MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYESGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLN
QVIEIKDLPQIDGSSMYLKNGESFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTSHDKITYKGQPINIKYDIVDGIK
TGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYLDSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKN
SYRIFLEKDESQGNYDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFLTENNVLMTFVKIIAGI
LVLLVLFIIASNIKKKKRKKARSKKNMKK

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=381, Percent_Identity=30.1837270341207, Blast_Score=148, Evalue=6e-37,
Organism=Escherichia coli, GI1787062, Length=394, Percent_Identity=31.4720812182741, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1786851, Length=352, Percent_Identity=30.1136363636364, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI87082054, Length=244, Percent_Identity=28.2786885245902, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 48261; Mature: 48261

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYESGKILYNKDGNSKLYP
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEECCCEEEEECCCCCEECC
ASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGESFTVKQLLDALLVHSAND
CCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEEEECCCCEEHHHHHHHHHHCCCCC
AAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHGLPDPNHYTTAHDMALIAREA
HHEEEEHHHCCCCHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
MNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTSHDKITYKGQPINIKYDIVDGIK
HCCHHHHHHHHHHCCEEEEEECCCCEEEEEECCCEEECCCEEEECCCEEEEEEEEECCCC
TGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYLDSRILIDYGFDNFKCATIVDKEKY
CCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCEEEECCEEEEEECCCCEEEEEEECCHHC
TDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGNYDTKTELNKIDLPIKKGAKVGTLNVY
CCHHHEEEEECCCEEECCCCCEEEEEEECCCCCCCCCCCCCCEECCCHHCCCEEEEEEEE
NNGKLENSIDLIAKNNLDSSLPFLTENNVLMTFVKIIAGILVLLVLFIIASNIKKKKRKK
CCCCCCCCEEEEEECCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARSKKNMKK
HHHHCCCCC
>Mature Secondary Structure
MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYESGKILYNKDGNSKLYP
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEECCCEEEEECCCCCEECC
ASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGESFTVKQLLDALLVHSAND
CCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEEEECCCCEEHHHHHHHHHHCCCCC
AAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHGLPDPNHYTTAHDMALIAREA
HHEEEEHHHCCCCHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
MNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTSHDKITYKGQPINIKYDIVDGIK
HCCHHHHHHHHHHCCEEEEEECCCCEEEEEECCCEEECCCEEEECCCEEEEEEEEECCCC
TGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYLDSRILIDYGFDNFKCATIVDKEKY
CCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCEEEECCEEEEEECCCCEEEEEEECCHHC
TDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGNYDTKTELNKIDLPIKKGAKVGTLNVY
CCHHHEEEEECCCEEECCCCCEEEEEEECCCCCCCCCCCCCCEECCCHHCCCEEEEEEEE
NNGKLENSIDLIAKNNLDSSLPFLTENNVLMTFVKIIAGILVLLVLFIIASNIKKKKRKK
CCCCCCCCEEEEEECCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARSKKNMKK
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]