Click here to switch to the map view.

The map label for this gene is pduQ [H]

Identifier: 126699517

GI number: 126699517

Start: 2208482

End: 2209612

Strand: Direct

Name: pduQ [H]

Synonym: CD1907

Alternate gene names: 126699517

Gene position: 2208482-2209612 (Clockwise)

Preceding gene: 126699516

Following gene: 126699518

Centisome position: 51.48

GC content: 29.27

Gene sequence:

>1131_bases
TTGAATAACTTTGAGATTAAAACTAGAATAAAATTTGGAGAAGGCTCACTAAAAGCTTTGCAAGAATTAAAAAACAAAAA
TGTACTAATAATAACAGACCCTTTTATGGTGAAATCAAAAACAATAGATAAAATAACACCTAATTTAATAAACTCAACAT
ATCAAGTTTTTGAAGAAATAGTACCAGACCCACCAATAGAAGTAGTAGTAGCAGGAATAGAAGCATTTAAATCTATAAAT
CCAGACGTAATAATAGCATTAGGTGGTGGTTCTGCAATAGATGCAGCAAAATCTATAATGGACTTTTCTAAAAAAATATT
AAAAATTAACGAAGTAGAGTTTATAGCAATACCAACAACAAGTGGAACAGGTTCAGAAGTAACATCATTTGCTGTAATAA
CAGATAGTCAAAAAAAATGTAAGTATCCATTAGTGAGTGATGATTTACTTCCAGATATGGCAATACTAGACCCAGAGCTT
GTAAAAACTGTACCAAATTTTATAACAGCAGATACAGGGATGGATGTATTGACACATGCTATAGAAGCTTACGTATCTAC
AAATTCTAATGACTTTTCAGATGCACTAGCTGAAAAAGCTATAAAGTTAGTATTTAAATACTTAATAAAAGCATATAAAG
ATGGAAATGACATAGAAGCTAGAGAAAAAATGCACAATGCATCTTGTTTAGCAGGTATGGCATTTAACCAAGCATCACTT
GGCATAAATCATTCTATTGCACATGTATTAGGTGGAAAATTCCATATACCACATGGAAGAACAAACTCTATATTACTTCC
TCATGTAATAGAATATAATTCATCAATAGTAGGATATACTAACACAACTTACTCTGATACAGCTAAAAAATATGCACAAA
TAGCAGAATTAGTAGGACTTGGGAGTGCTAATATAAGAATATCTGTTAGAAATTTAGTAAATGAAATAAAGAAACTACAA
AAAGAAATGAATATGCCAACTAAATTGACACAATGCAAATTAAGTTTAGAAGATATAACTAATGAAGAAAGAGAAATAGC
AAAACTTGCTATAAAAGATACTTGTACATTAACTAATCCTAAGGTACCAACTGAAGATGATATAATAAACATATTAAAGA
ACATTAAATAA

Upstream 100 bases:

>100_bases
TTTTTAAATATAGCACAATGAAGTGTTAAAAGAGCAATGGAGCTCCATAGATAAAGTCTATGGAGCTTTTTTGCGTATTT
AACTTAAGGAGGATAGGACT

Downstream 100 bases:

>100_bases
CTACATTTTATTACATGTAAATATAATATTATACAAATAGGGAAAATGTTTTTAAAAATATAAATTATATATTGACAAAT
ATAAAACATAAAACTATAAT

Product: ethanolamine/propanediol utilization propanol dehydrogenase

Products: Acetyl-CoA; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: BDH [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDKITPNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSIN
PDVIIALGGGSAIDAAKSIMDFSKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKAYKDGNDIEAREKMHNASCLAGMAFNQASL
GINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSIVGYTNTTYSDTAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQ
KEMNMPTKLTQCKLSLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILKNIK

Sequences:

>Translated_376_residues
MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDKITPNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSIN
PDVIIALGGGSAIDAAKSIMDFSKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKAYKDGNDIEAREKMHNASCLAGMAFNQASL
GINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSIVGYTNTTYSDTAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQ
KEMNMPTKLTQCKLSLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILKNIK
>Mature_376_residues
MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDKITPNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSIN
PDVIIALGGGSAIDAAKSIMDFSKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKAYKDGNDIEAREKMHNASCLAGMAFNQASL
GINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSIVGYTNTTYSDTAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQ
KEMNMPTKLTQCKLSLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILKNIK

Specific function: This enzyme has activity using butanol and ethanol as substrates [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=402, Percent_Identity=26.6169154228856, Blast_Score=102, Evalue=9e-22,
Organism=Escherichia coli, GI1787493, Length=399, Percent_Identity=38.3458646616541, Blast_Score=248, Evalue=4e-67,
Organism=Escherichia coli, GI87082107, Length=388, Percent_Identity=32.2164948453608, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI48994951, Length=360, Percent_Identity=35.8333333333333, Blast_Score=201, Evalue=8e-53,
Organism=Escherichia coli, GI1789163, Length=381, Percent_Identity=32.8083989501312, Blast_Score=195, Evalue=3e-51,
Organism=Escherichia coli, GI1789386, Length=361, Percent_Identity=24.9307479224377, Blast_Score=76, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17537053, Length=389, Percent_Identity=25.706940874036, Blast_Score=115, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6321181, Length=379, Percent_Identity=36.6754617414248, Blast_Score=223, Evalue=6e-59,
Organism=Drosophila melanogaster, GI24657991, Length=436, Percent_Identity=25.4587155963303, Blast_Score=99, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.1; 1.2.1.10 [C]

Molecular weight: Translated: 41301; Mature: 41301

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDKITPNLINSTYQVFEEI
CCCCEEEEEEEECCCHHHHHHHHCCCCEEEEECCCEECCCCHHHCCHHHHHHHHHHHHHH
VPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDFSKKILKINEVEFIAIPTT
CCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHEECCEEEEEEECC
SGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPELVKTVPNFITADTGMDVLTHA
CCCCCCCEEEEEEECCHHHCCCCCCCCCCCCCHHHCCHHHHHHHHHHHCCCCCHHHHHHH
IEAYVSTNSNDFSDALAEKAIKLVFKYLIKAYKDGNDIEAREKMHNASCLAGMAFNQASL
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHCCCHHC
GINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSIVGYTNTTYSDTAKKYAQIAELVGL
CCCHHHHHHHCCEEECCCCCCCCEECCHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHCC
GSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKLSLEDITNEEREIAKLAIKDTCTLTNP
CCCCEEEEHHHHHHHHHHHHHHCCCCCHHHHHEEEHHHHCCHHHHHHHHHHHCCEECCCC
KVPTEDDIINILKNIK
CCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDKITPNLINSTYQVFEEI
CCCCEEEEEEEECCCHHHHHHHHCCCCEEEEECCCEECCCCHHHCCHHHHHHHHHHHHHH
VPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDFSKKILKINEVEFIAIPTT
CCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHEECCEEEEEEECC
SGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPELVKTVPNFITADTGMDVLTHA
CCCCCCCEEEEEEECCHHHCCCCCCCCCCCCCHHHCCHHHHHHHHHHHCCCCCHHHHHHH
IEAYVSTNSNDFSDALAEKAIKLVFKYLIKAYKDGNDIEAREKMHNASCLAGMAFNQASL
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHCCCHHC
GINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSIVGYTNTTYSDTAKKYAQIAELVGL
CCCHHHHHHHCCEEECCCCCCCCEECCHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHCC
GSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKLSLEDITNEEREIAKLAIKDTCTLTNP
CCCCEEEEHHHHHHHHHHHHHHCCCCCHHHHHEEEHHHHCCHHHHHHHHHHHCCEECCCC
KVPTEDDIINILKNIK
CCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 1000 [C]

Specific activity: NA

Km value (mM): 0.15 {ethanol}} 7.05 {acetaldehyde}} 0.1 {acetaldehyde}} [C]

Substrates: Acetaldehyde; Coenzyme A; Nicotinamide adenine dinucleotide [C]

Specific reaction: Acetaldehyde + Coenzyme A + Nicotinamide adenine dinucleotide --> Acetyl-CoA + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: 8-Hydroxy quinoline-5-sulfonic acid; Dipicolinic acid; EDTA; Ethanol; GSH; Heptafluorbutanol; Imidazole; Iodoacetamide; Iodoacetate; Isobutyramide EDTA; NAD+; NADH; NEM; o-Phenanthroline of Zn2+; PCMB; Pyrazole; Substituted pyrazoles 4-iodo-; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2673928 [H]