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The map label for this gene is cysD [H]

Identifier: 126699433

GI number: 126699433

Start: 2110904

End: 2112169

Strand: Direct

Name: cysD [H]

Synonym: CD1825

Alternate gene names: 126699433

Gene position: 2110904-2112169 (Clockwise)

Preceding gene: 126699432

Following gene: 126699434

Centisome position: 49.2

GC content: 31.44

Gene sequence:

>1266_bases
ATGTATAATAAAGAAACAATATGTGTGCAAGGAAATTATAAACCAGGTAATGGAGAACCAAGAGTACTACCTTTATATCA
AAGTACAACTTTTAAATATAGCAGTATAGACCAACTTGCTGAATTATTTGATTTAAAAGTTGATGGACATATATATTCAA
GAATAAGCAATCCTACTATTCAAGCTTTTGAAGAAAAAATAAGTTTACTAGAGGGTGGAGTATCTTCTGTAGCTGTATCA
TCAGGGCAATCTGCAAATATGTTGGCAGTTTTAAATATATGTAAATCAGGAGATAGTATACTTTGTTCTTCAAAAGTATA
TGGAGGAACATTCAATTTACTAGGACCTAGTCTTAAAAAATTTGGTATAGATTTAATATCGTTTGACTTAGATTCAAGTG
AAGATGAGATAGTAGAACTTGCAAAGGAAAATACTAAGGTTGTGTTTGCAGAAACACTTGCAAATCCAACTCTTGAAGTC
ATAGATTTTGAAAAAATAGCAAATGTAGCTAAGAGAATTAATGTTCCATTTATTGTTGATAATTCATTAGCATCTCCAGT
GCTTTGTAACCCTTTAAAGTATGGAGCAAATATAGTTACTCATTCTACCACAAAATATTTAGATGGGCATGCTTCAAGTG
TTGGAGGAATTATAGTGGATGGTGGAAACTTTAACTGGGATAATGGAAAATTTCCAGAATTAGTTGAGCCAGACCCAACA
TATCATGGTATAAGCTATACTCAAAAATTTGGAAATGCCGCATATGCAACTAAAGCAAGAGTTCAGTTGCTTAGAGACTA
TGGAAATTGTTTAAGCCCATTCAATGCGTATCTTACTAATTTAAATGTTGAAACACTACATCTTAGAATGGAGAGACATA
GTGAAAATGCACTTAAAATAGCTAGATTTTTAGAAAAACATGAAAATGTAGATTGGATTAATTACCCAGGACTTGAAGAT
AACAAGTATTATGAGAATGCCAAAAAGTATTTATCAAGAGGATGTAGTGGTGTTTTATCATTTGGAGTAAGAGGTGGGTT
AGAAAATGCCAAAAAATTTGTGGAAAAATTACAGATAGCATCTTTGGTTACACATGTTTCAGATGTAAGAACTTGTGTTA
TACATCCAGCTTCAACTACTCATAGACAATTAACAGAAGAACAATTAATTGCATCTGGAGTATTGCCTTCACTAATAAGA
TTATCTGTTGGAATAGAAAATGTAGAGGATTTAATAGCTGATTTAAATCAAGCTTTAAATTTCTAA

Upstream 100 bases:

>100_bases
TTATACAGAGCCTGAGTTATATACTCAGGCTTTATTTATTTTAACTCTCATCTGGTAAATAGTTTAATTTTTTTTACTAA
AATCTAAGGGGGATACAAGA

Downstream 100 bases:

>100_bases
TATTCTAAGAGAAAGAGAAGTGAAAAGATATGGCTTTAATACTACCAAAGGGATTACCTGTTATTAATAAATTATTAGAT
GAAGGAATAGATGTAATTTA

Product: O-acetylhomoserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFDLKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVS
SGQSANMLAVLNICKSGDSILCSSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGIIVDGGNFNWDNGKFPELVEPDPT
YHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNAYLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLED
NKYYENAKKYLSRGCSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIASGVLPSLIR
LSVGIENVEDLIADLNQALNF

Sequences:

>Translated_421_residues
MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFDLKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVS
SGQSANMLAVLNICKSGDSILCSSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGIIVDGGNFNWDNGKFPELVEPDPT
YHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNAYLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLED
NKYYENAKKYLSRGCSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIASGVLPSLIR
LSVGIENVEDLIADLNQALNF
>Mature_421_residues
MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFDLKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVS
SGQSANMLAVLNICKSGDSILCSSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGIIVDGGNFNWDNGKFPELVEPDPT
YHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNAYLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLED
NKYYENAKKYLSRGCSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIASGVLPSLIR
LSVGIENVEDLIADLNQALNF

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=402, Percent_Identity=32.8358208955224, Blast_Score=182, Evalue=7e-46,
Organism=Homo sapiens, GI299473759, Length=402, Percent_Identity=29.6019900497512, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI25453487, Length=400, Percent_Identity=29, Blast_Score=135, Evalue=1e-31,
Organism=Escherichia coli, GI1790375, Length=394, Percent_Identity=29.9492385786802, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI1789383, Length=375, Percent_Identity=26.9333333333333, Blast_Score=117, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71980708, Length=414, Percent_Identity=32.8502415458937, Blast_Score=223, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI17560056, Length=403, Percent_Identity=32.7543424317618, Blast_Score=188, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI17538055, Length=376, Percent_Identity=32.7127659574468, Blast_Score=187, Evalue=6e-48,
Organism=Saccharomyces cerevisiae, GI6323333, Length=425, Percent_Identity=44.9411764705882, Blast_Score=337, Evalue=2e-93,
Organism=Saccharomyces cerevisiae, GI6319307, Length=403, Percent_Identity=34.9875930521092, Blast_Score=201, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6321170, Length=346, Percent_Identity=27.4566473988439, Blast_Score=116, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6321254, Length=440, Percent_Identity=27.7272727272727, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6321904, Length=429, Percent_Identity=24.7086247086247, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19922554, Length=399, Percent_Identity=32.0802005012531, Blast_Score=174, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46355; Mature: 46355

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFDLKVDGHIYSRISNPTI
CCCCCEEEEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCHH
QAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILCSSKVYGGTFNLLGPSLKK
HHHHHHHHHHHCCHHHEEECCCCCCCHHHHHHHHCCCCCEEEECCCCCCCHHHCCCHHHH
FGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEVIDFEKIANVAKRINVPFIVD
HCCEEEEECCCCCHHHHHHHHHCCCEEEEEHHHCCCCEEEECHHHHHHHHHHCCCCEEEC
NSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGIIVDGGNFNWDNGKFPELVEPDPT
CCCCCCHHCCCHHHCCEEEEECCHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC
YHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNAYLTNLNVETLHLRMERHSENALKI
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEHHCCCHHHHH
ARFLEKHENVDWINYPGLEDNKYYENAKKYLSRGCSGVLSFGVRGGLENAKKFVEKLQIA
HHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHH
SLVTHVSDVRTCVIHPASTTHRQLTEEQLIASGVLPSLIRLSVGIENVEDLIADLNQALN
HHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHC
F
C
>Mature Secondary Structure
MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFDLKVDGHIYSRISNPTI
CCCCCEEEEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCHH
QAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILCSSKVYGGTFNLLGPSLKK
HHHHHHHHHHHCCHHHEEECCCCCCCHHHHHHHHCCCCCEEEECCCCCCCHHHCCCHHHH
FGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEVIDFEKIANVAKRINVPFIVD
HCCEEEEECCCCCHHHHHHHHHCCCEEEEEHHHCCCCEEEECHHHHHHHHHHCCCCEEEC
NSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGIIVDGGNFNWDNGKFPELVEPDPT
CCCCCCHHCCCHHHCCEEEEECCHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC
YHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNAYLTNLNVETLHLRMERHSENALKI
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEHHCCCHHHHH
ARFLEKHENVDWINYPGLEDNKYYENAKKYLSRGCSGVLSFGVRGGLENAKKFVEKLQIA
HHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHH
SLVTHVSDVRTCVIHPASTTHRQLTEEQLIASGVLPSLIRLSVGIENVEDLIADLNQALN
HHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHC
F
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]