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The map label for this gene is thiC

Identifier: 126699308

GI number: 126699308

Start: 1976366

End: 1977667

Strand: Direct

Name: thiC

Synonym: CD1702

Alternate gene names: 126699308

Gene position: 1976366-1977667 (Clockwise)

Preceding gene: 126699307

Following gene: 126699309

Centisome position: 46.07

GC content: 31.95

Gene sequence:

>1302_bases
ATGAACTATACAACACAAATGGATGCTGCTAGAAAAGGCATAATAACTAAAGAAATGGAAATCGTTTCTCAAAAAGAACA
GGTAGATGTAAATGAATTAAGAGAATTAATAGCAAATGGACAAGTTGTTATACCAGCTAATAAAAATCATAAATCTTTAA
GTGCAGAAGGTGTAGGTAAAAATTTAAGAACAAAAATAAATGTAAATCTAGGAATTTCAAGAGATTGTAAGGATATAGAA
AAAGAACTTGAAAAAGTTAGAGTAGCAATAGACATGAAAGCTGAAGCTATAATGGATTTAAGTAATTATGGCAAAACTAG
AGAATTTAGAGAAAAGGTTGTTGAAATGTCACCTGCAATGATAGGTTCAGTACCTATGTATGATGCAGTAGGATATTTGG
AAAAAGAATTAAAAGATATAACAGAAGAAGAGTTTTTAAATGTAATAAGACAACATGCTATTGATGGAGTAGACTTTATT
ACTATACATGCAGGTCTTACAAGAAGTGTTTGCCAAAAAATAAAAAATCATGAGAGATTGACTCATATAGTATCTCGTGG
AGGTTCTTTATTGTTTGCATGGATGGAATTAAATAATAAGGAAAATCCTATTTATACTAATTTTGATAAAATATTGGATA
TATGTGAAGAATATGATGTTACATTGAGTTTAGGAGATGCTTGTAGACCAGGTTGTATAAAAGATTCTACTGATGGTGTT
CAAATTCAAGAACTTGTAGTATTAGGTGAACTTACTAAAAGAGCATGGGAAAGAAATGTACAAGTAATGATAGAAGGTCC
TGGACACATGGCTATTGATGAAATAGAAGCAAATGTAGTATTAGAAAAGAGATTATGCCATGGAGCACCTTTTTATGTTC
TTGGACCATTGGTTACAGATATAGCACCAGGTTATGACCATATAACAAGTGCAATAGGAGGAGCTTTAGCATGTGCCAAA
GGAGTAGATTTTTTATGTTATGTAACTCCAGCAGAACATTTAAGATTGCCTAATTTAGATGATATGAAAGAGGGTATAAT
AGCTGCAAAGATAGCTGCTCATGCAGGAGATATAGCTAAAAATGTTAAAGGTGCTCGTGAATGGGATAATAAGATGAGTA
AAGCTAGAGCTGATTTAGACTGGTGTGAGATGTTTAGACTTGCAATAGACCCTGAAAAAGCTAAGAGATACAGAGATGAA
TCAACTCCTACTCATGAGGATAGTTGTACTATGTGTGGAAAAATGTGCTCAATGAGAACAGTTAAGAAAATATTAAACAA
TGAGGAACTTAATCTAATATAA

Upstream 100 bases:

>100_bases
TTGTCTAAGTGTATATACGATTATAAGAGTGCTTTCCAAACGAGGAAAAGCACTTTTTTTATTTTCAAAAACTATAACTT
TAATCTTGGAGGATTGTAAT

Downstream 100 bases:

>100_bases
TTTTTGGCTAGTATGACTGGAATAAAAATAAGCTGAAAAGAGGAGGTGATTTAGTGAAGGTTAATGGGAAGGAAATTGAA
TTTGAAAAAGATTTGACTGT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVIPANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIE
KELEKVRVAIDMKAEAIMDLSNYGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEYDVTLSLGDACRPGCIKDSTDGV
QIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIEANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAK
GVDFLCYVTPAEHLRLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPEKAKRYRDE
STPTHEDSCTMCGKMCSMRTVKKILNNEELNLI

Sequences:

>Translated_433_residues
MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVIPANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIE
KELEKVRVAIDMKAEAIMDLSNYGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEYDVTLSLGDACRPGCIKDSTDGV
QIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIEANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAK
GVDFLCYVTPAEHLRLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPEKAKRYRDE
STPTHEDSCTMCGKMCSMRTVKKILNNEELNLI
>Mature_433_residues
MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVIPANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIE
KELEKVRVAIDMKAEAIMDLSNYGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEYDVTLSLGDACRPGCIKDSTDGV
QIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIEANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAK
GVDFLCYVTPAEHLRLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPEKAKRYRDE
STPTHEDSCTMCGKMCSMRTVKKILNNEELNLI

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=453, Percent_Identity=44.3708609271523, Blast_Score=370, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_CLOD6 (Q186R0)

Other databases:

- EMBL:   AM180355
- RefSeq:   YP_001088205.1
- STRING:   Q186R0
- GeneID:   4916741
- GenomeReviews:   AM180355_GR
- KEGG:   cdf:CD1702
- NMPDR:   fig|1496.1.peg.1670
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   VDFMTIH
- ProtClustDB:   PRK13352
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 48451; Mature: 48451

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: BINDING 69-69 BINDING 98-98 BINDING 127-127 BINDING 163-163 BINDING 265-265 BINDING 292-292

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVIPANKNHKSLSAEGVGK
CCCCCCHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCCC
NLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSNYGKTREFREKVVEMSPAM
CCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHCHHH
IGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFITIHAGLTRSVCQKIKNHERL
HCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHH
THIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEYDVTLSLGDACRPGCIKDSTDGV
HHHHHCCCCEEEEEEECCCCCCCCEECHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCC
QIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIEANVVLEKRLCHGAPFYVLGPLVTD
CHHHHHHHHHHHHHHHCCCCEEEEECCCCEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
IAPGYDHITSAIGGALACAKGVDFLCYVTPAEHLRLPNLDDMKEGIIAAKIAAHAGDIAK
CCCCHHHHHHHHHHHHHHHCCCCEEEEECCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
NVKGAREWDNKMSKARADLDWCEMFRLAIDPEKAKRYRDESTPTHEDSCTMCGKMCSMRT
HCCCHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
VKKILNNEELNLI
HHHHHCCCCCCCC
>Mature Secondary Structure
MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVIPANKNHKSLSAEGVGK
CCCCCCHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCCC
NLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSNYGKTREFREKVVEMSPAM
CCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHCHHH
IGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFITIHAGLTRSVCQKIKNHERL
HCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHH
THIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEYDVTLSLGDACRPGCIKDSTDGV
HHHHHCCCCEEEEEEECCCCCCCCEECHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCC
QIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIEANVVLEKRLCHGAPFYVLGPLVTD
CHHHHHHHHHHHHHHHCCCCEEEEECCCCEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
IAPGYDHITSAIGGALACAKGVDFLCYVTPAEHLRLPNLDDMKEGIIAAKIAAHAGDIAK
CCCCHHHHHHHHHHHHHHHCCCCEEEEECCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
NVKGAREWDNKMSKARADLDWCEMFRLAIDPEKAKRYRDESTPTHEDSCTMCGKMCSMRT
HCCCHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
VKKILNNEELNLI
HHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA