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The map label for this gene is exoA [H]

Identifier: 126698940

GI number: 126698940

Start: 1557253

End: 1558005

Strand: Direct

Name: exoA [H]

Synonym: CD1341

Alternate gene names: 126698940

Gene position: 1557253-1558005 (Clockwise)

Preceding gene: 126698939

Following gene: 126698942

Centisome position: 36.3

GC content: 29.22

Gene sequence:

>753_bases
ATGAAATTTATATCATGGAATGTTAATGGAATAAGAGCTTGTGTTGGGAAAGGATTTTTAGACTTTTTCAAAGAAGTAGA
TGCAGATATATTTTGTTTACAAGAAACTAAATTGCAAGAAGGGCAAATAGAGTTAGATTTACCAGGATATTTTCAATATT
GGAATTATGCTGAGAGAAAGGGTTATTCAGGTACTGCTATATTTACAAAAAAAGAGCCTTTAAAAGTAATGTATGGAATA
AATATAGAAGAACATGACAAAGAGGGTAGAGTGATAACTCTAGAGTTTGAAGATTTTTACTTTGTTACAGTATATACGCC
AAATTCTCAATCTGAATTAAAGAGACTAGAATATAGAACTAGATGGGAAGACGACTTTATAGATTATTTAACCAAACTAG
ATAATCATAAGCCTGTAATTGTGTGTGGAGATATGAACGTTGCACATAAAGAAATAGATTTAAAAAATCCTAAAAATAAT
ATGAAAAATGCAGGTTTTACTAAAGAAGAAAGAGAGAAGTTTTCTAAATTATTAGACAGTGGGTTTATAGACACATATAG
ATATTTTAATCCTGATAAAGAAGGTGTTTATAGTTGGTGGTCATATAGATTTAATGCAAGAAAAAATAATGCAGGGTGGA
GAATAGATTATTTTTGTGCATCCAAAAAATTAGAAGATAGATTAGTAAGTGCGGATATACACACTGAAATATTAGGTTCA
GACCATTGTCCAGTTGAATTAGAGATAAAATAA

Upstream 100 bases:

>100_bases
TGTTAAAATAAAAGTGAATTAAGAATTTACAATAAAATTGTTGAAAAGCCCCATCTTTAGCTATGGGGAGTGTGTCAATG
TTTAGATGAGGAGTATAAGT

Downstream 100 bases:

>100_bases
AAATTAATTATGATAAAAATTGGGCTGTAGATATAAATTTAAAGTTAACTAGTTTTTTAATCTACATGCCTATTTTTTAT
CTAAATAAACTCTATTTGTT

Product: exodeoxyribonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIELDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGI
NIEEHDKEGRVITLEFEDFYFVTVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYFCASKKLEDRLVSADIHTEILGS
DHCPVELEIK

Sequences:

>Translated_250_residues
MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIELDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGI
NIEEHDKEGRVITLEFEDFYFVTVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYFCASKKLEDRLVSADIHTEILGS
DHCPVELEIK
>Mature_250_residues
MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIELDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGI
NIEEHDKEGRVITLEFEDFYFVTVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYFCASKKLEDRLVSADIHTEILGS
DHCPVELEIK

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=253, Percent_Identity=48.6166007905138, Blast_Score=257, Evalue=8e-69,
Organism=Homo sapiens, GI18375503, Length=253, Percent_Identity=48.6166007905138, Blast_Score=257, Evalue=8e-69,
Organism=Homo sapiens, GI18375501, Length=253, Percent_Identity=48.6166007905138, Blast_Score=257, Evalue=8e-69,
Organism=Homo sapiens, GI18375507, Length=310, Percent_Identity=27.741935483871, Blast_Score=106, Evalue=2e-23,
Organism=Escherichia coli, GI1788046, Length=263, Percent_Identity=31.1787072243346, Blast_Score=117, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI71989536, Length=256, Percent_Identity=44.140625, Blast_Score=209, Evalue=8e-55,
Organism=Caenorhabditis elegans, GI71989539, Length=146, Percent_Identity=40.4109589041096, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI221330655, Length=250, Percent_Identity=50, Blast_Score=263, Evalue=1e-70,
Organism=Drosophila melanogaster, GI17136678, Length=250, Percent_Identity=50, Blast_Score=263, Evalue=1e-70,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29560; Mature: 29560

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIELDLPGYFQYWNYAERK
CEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEECCCHHHHCCHHHHC
GYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFVTVYTPNSQSELKRLEYRT
CCCCEEEEECCCCEEEEEECCCHHCCCCCCEEEEEECCEEEEEEECCCCHHHHHHHHHHC
RWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNNMKNAGFTKEEREKFSKLLDS
CCCHHHHHHHHHHCCCCCEEEECCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHC
GFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYFCASKKLEDRLVSADIHTEILGS
CCCCHHHHCCCCCCHHEEEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHCCHHHHHCCC
DHCPVELEIK
CCCCEEEEEC
>Mature Secondary Structure
MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIELDLPGYFQYWNYAERK
CEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEECCCHHHHCCHHHHC
GYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFVTVYTPNSQSELKRLEYRT
CCCCEEEEECCCCEEEEEECCCHHCCCCCCEEEEEECCEEEEEEECCCCHHHHHHHHHHC
RWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNNMKNAGFTKEEREKFSKLLDS
CCCHHHHHHHHHHCCCCCEEEECCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHC
GFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYFCASKKLEDRLVSADIHTEILGS
CCCCHHHHCCCCCCHHEEEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHCCHHHHHCCC
DHCPVELEIK
CCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]