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The map label for this gene is truB

Identifier: 126698912

GI number: 126698912

Start: 1523747

End: 1524652

Strand: Direct

Name: truB

Synonym: CD1314

Alternate gene names: 126698912

Gene position: 1523747-1524652 (Clockwise)

Preceding gene: 126698911

Following gene: 126698913

Centisome position: 35.52

GC content: 27.15

Gene sequence:

>906_bases
ATGAATAAGATTATAAGTATATTGAAACCCACAGGTATGACATCTCATGATGTTGTAAGTAGAGTGAGAAAGATTTTAAA
TATAAAAAAAGTTGGTCATACAGGTACTCTTGACCCAGATGCTTCAGGTGTTTTACCAATCTGTATAGGGAAAGCTACAA
AAGTATGTGAAGTTATTTTAAACAAAGATAAATCGTATATATGTGAGCTTACTTTAGGTATAAGTACAGATACTTATGAT
GCTTCAGGTGAAATTTTAAAAAAAGTTGATGATTTTAAATTTAGTAATGAAGACATAGAAAGAGCTTTTGACACACAAAG
AGGAGAGATAAATCAACTTCCTCCAATTTATTCAGCTTTAAAGGTAAATGGAAAGAGAATGTGTGATTTAGTAAGAAGTG
GTAGACAATCTGAAATTACTTTAAAAACAAGAAGAGTAAATATAAAAGACATAAAAATACTAAGTATAAAAGGTAATAAA
GTAATGTTTTATGTTGAGTGTTCTAAAGGGACTTATGTGAGAAGCATTTGCCATGATATAGGAGAATATCTAGGTTGTGG
AGCTCATATGTCGTTTTTGAATAGAACATCTTCTGGTAAATTTGACTTAGATAATTCAATAACATTAGAAGAATTAGAAT
TATTTTATGAAAATAAAACTTTAGACAAATACTTATATGATATAGATTATGTCCTTGATTCGTTTAATTATGTTGTATTA
AACCCTAATGCTATAAAATATTATAGTAATGGTGGCTCAATAGATGATAAAAGGTTTTTAAAAAATAATTTCGATAAAGA
TGATGAATTTGTAAGAGTATACAGTACAGATAATTTCTTAGGATTAGGAAAATTATCAAAACACAATAATACAATAAGTG
TTAAAAGTGATAAGATGTTCATATAA

Upstream 100 bases:

>100_bases
TCATGTAAGGGCTGCTGGATGTACTATTAGAGATAGTATTGATAATGCAAAGAAAATGGTACTAGAGGCAGTGTTAAAAT
CAATTTAAGGTGACAGTAAA

Downstream 100 bases:

>100_bases
CCAATGTATTTAAAAAAACAACAAAAGAGATAGTACTATAATAACTTCTATAGTCTATCTCTTTTACTTATGCTATAATA
GTTTGTGAATAAGTTAAGAG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGVLPICIGKATKVCEVILNKDKSYICELTLGISTDTYD
ASGEILKKVDDFKFSNEDIERAFDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYENKTLDKYLYDIDYVLDSFNYVVL
NPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYSTDNFLGLGKLSKHNNTISVKSDKMFI

Sequences:

>Translated_301_residues
MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGVLPICIGKATKVCEVILNKDKSYICELTLGISTDTYD
ASGEILKKVDDFKFSNEDIERAFDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYENKTLDKYLYDIDYVLDSFNYVVL
NPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYSTDNFLGLGKLSKHNNTISVKSDKMFI
>Mature_301_residues
MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGVLPICIGKATKVCEVILNKDKSYICELTLGISTDTYD
ASGEILKKVDDFKFSNEDIERAFDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYENKTLDKYLYDIDYVLDSFNYVVL
NPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYSTDNFLGLGKLSKHNNTISVKSDKMFI

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=224, Percent_Identity=34.8214285714286, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI4503337, Length=260, Percent_Identity=28.0769230769231, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI215599015, Length=260, Percent_Identity=28.0769230769231, Blast_Score=92, Evalue=4e-19,
Organism=Escherichia coli, GI2367200, Length=296, Percent_Identity=35.472972972973, Blast_Score=193, Evalue=9e-51,
Organism=Caenorhabditis elegans, GI17553978, Length=205, Percent_Identity=31.7073170731707, Blast_Score=94, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6323204, Length=236, Percent_Identity=30.0847457627119, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6324037, Length=136, Percent_Identity=37.5, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI281364189, Length=279, Percent_Identity=26.1648745519713, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI281364187, Length=279, Percent_Identity=26.1648745519713, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI281364185, Length=279, Percent_Identity=26.1648745519713, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI281364183, Length=279, Percent_Identity=26.1648745519713, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI62471759, Length=279, Percent_Identity=26.1648745519713, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI17975520, Length=279, Percent_Identity=26.1648745519713, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_CLOD6 (Q18BH7)

Other databases:

- EMBL:   AM180355
- RefSeq:   YP_001087809.1
- ProteinModelPortal:   Q18BH7
- SMR:   Q18BH7
- STRING:   Q18BH7
- GeneID:   4914473
- GenomeReviews:   AM180355_GR
- KEGG:   cdf:CD1314
- NMPDR:   fig|1496.1.peg.3418
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   YPAHMSK
- ProtClustDB:   CLSK2534806
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 34092; Mature: 34092

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: NA

Important sites: ACT_SITE 38-38

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGVLPICIGKATKVCEVIL
CCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHHHHHHH
NKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERAFDTQRGEINQLPPIYSAL
CCCCCEEEEEEEECCCCCCCCCHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCHHHHHH
KVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNKVMFYVECSKGTYVRSICHDI
HCCHHHHHHHHHCCCCCEEEEEEEEECEEEEEEEEEECCEEEEEEECCCCHHHHHHHHHH
GEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYENKTLDKYLYDIDYVLDSFNYVVL
HHHHCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEE
NPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYSTDNFLGLGKLSKHNNTISVKSDKMF
CCCEEEEECCCCCCCHHHHHHCCCCCCCCEEEEEECCCEEECCCCCCCCCEEEEECCCCC
I
C
>Mature Secondary Structure
MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGVLPICIGKATKVCEVIL
CCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHHHHHHH
NKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERAFDTQRGEINQLPPIYSAL
CCCCCEEEEEEEECCCCCCCCCHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCHHHHHH
KVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNKVMFYVECSKGTYVRSICHDI
HCCHHHHHHHHHCCCCCEEEEEEEEECEEEEEEEEEECCEEEEEEECCCCHHHHHHHHHH
GEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYENKTLDKYLYDIDYVLDSFNYVVL
HHHHCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEE
NPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYSTDNFLGLGKLSKHNNTISVKSDKMF
CCCEEEEECCCCCCCHHHHHHCCCCCCCCEEEEEECCCEEECCCCCCCCCEEEEECCCCC
I
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA