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The map label for this gene is dhaB1 [H]

Identifier: 126698712

GI number: 126698712

Start: 1316081

End: 1318453

Strand: Direct

Name: dhaB1 [H]

Synonym: CD1120

Alternate gene names: 126698712

Gene position: 1316081-1318453 (Clockwise)

Preceding gene: 126698711

Following gene: 126698713

Centisome position: 30.68

GC content: 31.61

Gene sequence:

>2373_bases
ATGAGTCAAACTAATAGAATAGAAGCATTTAGACAAGAATATATTAATTCTAAACCTATGATATGTTGTGAAAGGGCTAG
AATTTTTACAGAATCACATAAAAAAACTGAGGGAGAAGCTATCTGTATAAGAAGAGCTAAAGCTTTTTTGGAGACATGTA
AAGAACTTCCTATAAAAATCTTTGAAAATGAATTAATAGTAGGTACAGCAGGAAAATTTAGAAGAACAGGGATATTGACA
CCAGAGTTTTCTTGGCAATGGGTAGACAAAGAAATGGATACTTTTGATAAAAGAACTCAAGACCCATATGTAATATCAAA
AGAACAAATAGAATTTATAAGAAAAGAAATATTTCCATATTGGAAGGGAAAATCTTTGGAAGAAGTTTTCTTAGCAAGAA
TCCCAGAAGATACAGCTAAGATATTAGTAGATACAGGAATAATAGATAATGATTCTAAGTGGAGACAGGCAGTTGGTGAA
GTAACACCAGATTATCAAGATATATTGTTTGTAAAAGGATATAAAGGGATTAAGGAAGATGCAGATAAAAAAATTAAAGA
ATTAGATATATCAGTTTCAGAAAATATTGAAAAGATAGATTTTTACAAATCTGTTTCTATAGTTGCACAGGGTATTATGA
CATTGGCACAAAGATATTCAAATCTTGCAAAAGAGATGAGTAAGCAAGAAACAGATGAAAAGAGAAAGTTAGAACTTATA
AAAATTTCAGAGATATGTATGAATGTACCAGCAAACCCTCCAACTAATTTTTATGAAGCAATACAATTTGTATGGTTTGT
TCAATTAGGTGGTATTCTATCAGAAAATCCATTGGCATTAAATTTAGGTAGATTTGATCAATATATGTATCCATATTATG
AAAATGATGCTAGAGAAGGTAAAATAACTGAATCAGAAGCTCAAGAATTGATAGAAGCACTTTGGATTAAGTTATCAGAA
TGGGTATGGACTATATCAGCAAATACAGCAAATTACTTTGCAGGATATAATCAATTCCAAAATCTTACTGTTGGTGGTAA
AAAGAGAAATGGTACTGATGGAACGAATGATATCTCATATATGTGTTTAAAGGCTACAGAAAGTGTAAAAACTCACCAAC
CAGGATTAAGTGTTAGAGTGAGTCAAGGAGCACCAGACAATTTCGTAATGGCAGTTGCTAAATTGGTAAAACAAGGAACT
GGTTTCCCTGCTATACACAGCGATAGTGCAGGGGCACAAATGTTATTACAAGATGGATATGATGCAGAAGATGCTAGAGA
TTGGAGTAACTGTGGTTGTGTAGTTCCTCATTTTAGAAAAACAGGACAGTGGACTTCAGCCGTAAATATCAATTTTGCAG
CAGCTTTAGAATATGCTATGAATGAAGGTAAGAGTAGATTAACTGGAGAAAAAATGGGGTTAGATACAAAAAATATCACA
GAATTTACTTCTTTTGAAGAACTTAAGGATGAGTTTTTAAAGCAATTAGCATATCTTGTAAAAAGTTCTGTTATAGGAAC
TACTGTTGCTCAACAAATTCATAAAGAAATGGTACCAAGACCATTTTTATCTACTTGTGTAGATGGATGTTTGGATAAGG
GAGTTGATTTAAGTAAAGGAGGAGCAAAATATAATATAGGTCCTGTATTAACTGGTATAGGGTTAGGTGTAGTTTCAAAT
TCATTGGCAGCTATAAAAAAATTAGTATTTGAGGATAAGGTGACTACGTTAGAAGAATTAACAAAAGCACTAAATAATGA
TTGGGAAGGTTATGAAGAATTAAGAAAACTTGCGTTGGATGTTCCTAAGTATGGAAATGATAATGATTATGTAGATTCAT
TAGCAATTGAAGTTTCTGATTTTTATTATACTGAAACTAGAAAATACAAGGATATTTTTGGCTCTAAATTTAATAGTGCA
TTTATGGGTATATCAAACTATGTACCAACTGGAAAAATAGTAGGTGCAACTCCTTGTGGAAGAAAAGCGACAAAACCTTT
GACAGAGGGAGTTTCTCCATTTGTTGGTACTGATACAACAAGTCCACTAGCAGCAATGAAATCTGCATCAAAAATAAATC
ATGATGTTCATACTGGTGGAACACTTCTAAATTTAAGACTGAATCAAGATTTGGTGGAAACTGAAAGAGGACTTAGAAAT
CTAACATCTATGATTAAATCATATTTTGCTTTAGGAGGATTCCATGTACAATTTAATACGATATCAAATGATACTCTATT
AAAAGCTCAAGAAAATCCAGAAGAATATAAAGATTTATTAGTGAGGGTTGCTGGATATAGTACTCAATTTGTTAATTTAT
CAAGAGAAATGCAAGATGCTATAATAGCTAGAAATTCACATAGCAATTTTTAA

Upstream 100 bases:

>100_bases
TTATTTTGCTTCCATTTTATGCTAAAGGACAGTATAATATAATTAAAGTATGTTATAACATACAAAAATGTAGTATATAT
TATCCAAAGGGGGATTTTAA

Downstream 100 bases:

>100_bases
TTATGAGGTAGATTATGAGTAAAAAAGGTAGAGTTGTAAAAGTACAACATTTTTCAGTAAATGATGGAGATGGAATAAGG
ACTACTATATTTTTAGAAGG

Product: glycerol dehydratase

Products: NA

Alternate protein names: Pyruvate formate-lyase 2 [H]

Number of amino acids: Translated: 790; Mature: 789

Protein sequence:

>790_residues
MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIRRAKAFLETCKELPIKIFENELIVGTAGKFRRTGILT
PEFSWQWVDKEMDTFDKRTQDPYVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQRYSNLAKEMSKQETDEKRKLELI
KISEICMNVPANPPTNFYEAIQFVWFVQLGGILSENPLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSE
WVWTISANTANYFAGYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAVAKLVKQGT
GFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNINFAAALEYAMNEGKSRLTGEKMGLDTKNIT
EFTSFEELKDEFLKQLAYLVKSSVIGTTVAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSN
SLAAIKKLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYTETRKYKDIFGSKFNSA
FMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSPLAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRN
LTSMIKSYFALGGFHVQFNTISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF

Sequences:

>Translated_790_residues
MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIRRAKAFLETCKELPIKIFENELIVGTAGKFRRTGILT
PEFSWQWVDKEMDTFDKRTQDPYVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQRYSNLAKEMSKQETDEKRKLELI
KISEICMNVPANPPTNFYEAIQFVWFVQLGGILSENPLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSE
WVWTISANTANYFAGYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAVAKLVKQGT
GFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNINFAAALEYAMNEGKSRLTGEKMGLDTKNIT
EFTSFEELKDEFLKQLAYLVKSSVIGTTVAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSN
SLAAIKKLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYTETRKYKDIFGSKFNSA
FMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSPLAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRN
LTSMIKSYFALGGFHVQFNTISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF
>Mature_789_residues
SQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIRRAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTP
EFSWQWVDKEMDTFDKRTQDPYVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGEV
TPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQRYSNLAKEMSKQETDEKRKLELIK
ISEICMNVPANPPTNFYEAIQFVWFVQLGGILSENPLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEW
VWTISANTANYFAGYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAVAKLVKQGTG
FPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNINFAAALEYAMNEGKSRLTGEKMGLDTKNITE
FTSFEELKDEFLKQLAYLVKSSVIGTTVAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNS
LAAIKKLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYTETRKYKDIFGSKFNSAF
MGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSPLAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNL
TSMIKSYFALGGFHVQFNTISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1790388, Length=793, Percent_Identity=34.4262295081967, Blast_Score=449, Evalue=1e-127,
Organism=Escherichia coli, GI1787044, Length=805, Percent_Identity=31.9254658385093, Blast_Score=426, Evalue=1e-120,
Organism=Escherichia coli, GI1787131, Length=547, Percent_Identity=24.6800731261426, Blast_Score=156, Evalue=6e-39,
Organism=Escherichia coli, GI48994926, Length=573, Percent_Identity=23.5602094240838, Blast_Score=138, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184
- InterPro:   IPR010098 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 88806; Mature: 88675

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIRRAKAFLETCKELPIKI
CCCCHHHHHHHHHHCCCCCCEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEE
FENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDPYVISKEQIEFIRKEIFPY
ECCCEEEECCCCCHHCCCCCCCCCCEEHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCC
WKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGEVTPDYQDILFVKGYKGIKED
CCCCCHHHHHHHHCCCHHHHEEEECCCCCCCHHHHHHHHCCCCCHHHEEEECCCCCCHHH
ADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQRYSNLAKEMSKQETDEKRKLELI
HHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
KISEICMNVPANPPTNFYEAIQFVWFVQLGGILSENPLALNLGRFDQYMYPYYENDAREG
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHCCCCCCCCCCCC
KITESEAQELIEALWIKLSEWVWTISANTANYFAGYNQFQNLTVGGKKRNGTDGTNDISY
CCCHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHE
MCLKATESVKTHQPGLSVRVSQGAPDNFVMAVAKLVKQGTGFPAIHSDSAGAQMLLQDGY
EEEHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCC
DAEDARDWSNCGCVVPHFRKTGQWTSAVNINFAAALEYAMNEGKSRLTGEKMGLDTKNIT
CCCCCCCHHCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHCCCHHCCCHHHCCCCCCHH
EFTSFEELKDEFLKQLAYLVKSSVIGTTVAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCC
GAKYNIGPVLTGIGLGVVSNSLAAIKKLVFEDKVTTLEELTKALNNDWEGYEELRKLALD
CCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
VPKYGNDNDYVDSLAIEVSDFYYTETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCG
CCCCCCCCCHHHHHHEEHHHHEEHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEECCCCC
RKATKPLTEGVSPFVGTDTTSPLAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRN
CCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHH
LTSMIKSYFALGGFHVQFNTISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDA
HHHHHHHHHHHCCEEEEEEECCCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
IIARNSHSNF
HHHCCCCCCC
>Mature Secondary Structure 
SQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIRRAKAFLETCKELPIKI
CCCHHHHHHHHHHCCCCCCEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEE
FENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDPYVISKEQIEFIRKEIFPY
ECCCEEEECCCCCHHCCCCCCCCCCEEHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCC
WKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGEVTPDYQDILFVKGYKGIKED
CCCCCHHHHHHHHCCCHHHHEEEECCCCCCCHHHHHHHHCCCCCHHHEEEECCCCCCHHH
ADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQRYSNLAKEMSKQETDEKRKLELI
HHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
KISEICMNVPANPPTNFYEAIQFVWFVQLGGILSENPLALNLGRFDQYMYPYYENDAREG
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHCCCCCCCCCCCC
KITESEAQELIEALWIKLSEWVWTISANTANYFAGYNQFQNLTVGGKKRNGTDGTNDISY
CCCHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHE
MCLKATESVKTHQPGLSVRVSQGAPDNFVMAVAKLVKQGTGFPAIHSDSAGAQMLLQDGY
EEEHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCC
DAEDARDWSNCGCVVPHFRKTGQWTSAVNINFAAALEYAMNEGKSRLTGEKMGLDTKNIT
CCCCCCCHHCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHCCCHHCCCHHHCCCCCCHH
EFTSFEELKDEFLKQLAYLVKSSVIGTTVAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCC
GAKYNIGPVLTGIGLGVVSNSLAAIKKLVFEDKVTTLEELTKALNNDWEGYEELRKLALD
CCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
VPKYGNDNDYVDSLAIEVSDFYYTETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCG
CCCCCCCCCHHHHHHEEHHHHEEHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEECCCCC
RKATKPLTEGVSPFVGTDTTSPLAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRN
CCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHH
LTSMIKSYFALGGFHVQFNTISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDA
HHHHHHHHHHHCCEEEEEEECCCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
IIARNSHSNF
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]