Click here to switch to the map view.

The map label for this gene is manZ [H]

Identifier: 126698665

GI number: 126698665

Start: 1270360

End: 1271202

Strand: Direct

Name: manZ [H]

Synonym: CD1078

Alternate gene names: 126698665

Gene position: 1270360-1271202 (Clockwise)

Preceding gene: 126698664

Following gene: 126698667

Centisome position: 29.61

GC content: 32.27

Gene sequence:

>843_bases
ATGACAACGAGTTCTAAGAAATTAGAGACAATTTCACCAGATAGTAAAATAACGAGGAAAGACTTTTGGAAATGTTTTAG
AAGGTCATTGACATTAGATTCATCTTGGAACTACGAACGTATGCAAAATATAGCATATGCATACATGATGGCACCAATAA
TCCGTAGATTATATAAAGATGATAAAGAAAAGAAATCTAAGGCTTTAAAAAGACATTTAGAATTTATGTCAGTTACTCCT
CATATAAGTACTCTTTTGGTAGGTATATCTGGAGCTATGGAAGAAGAAAATGCAAAGAATAAAGAATTTGATGCTAATAG
TATAAATGCTGTTAAATCAAGTTTAATGGGTCCAGTTTCTGGGATAGGAGATTCATTTTTCTGGGGAACATTAAAGCTTA
TAGCAGCTGGGGTTGGTATAGCTTTAGCTTCTCAGGGTAACATAATGGGACCGATTTTATTCCTTTTAATAATAAATGTT
CCTCATTTTATTATACGCTATATATGTCTAGATAAAGGATTTAAGTATGGAACTCAATTCTTTAAAGATGTGAGTGGTTC
AAGTATAGTTTCAAAAGTTATGGAAGCAGCTTCAATGTTAGGGCTTATGGTAATTGGAGGTATGACTGCATCTAATGTAA
TGTTAAAACTTAGTGTTAATGTAGGTAGTGGAGAATGGGCAGAGCCTATTCAAACTTACTTAGACCAAATAATGCCATGT
ATGCTTCCAGCTATGATATTCGGTATTATGTATTGGTTATTAGGAAAAAAGGTAAAGACAACTACGATATTAATTTCAGT
TATGATTATTTGTATAGTATTGGCTGCTATTGGAGTAGTATAA

Upstream 100 bases:

>100_bases
GTTGCTTACCTAAACATAACAGTAACAGGAGTTGCATTGTTTGGATTAGCAATAGCTTTAATATATGTTAACTTTGTAGG
AGAGAAGGAGGTAATAGTAG

Downstream 100 bases:

>100_bases
ATAAAAAAATCCCAAGTTCATTTGAACGAGGGATTTTTTGACTAAATTAAATAAAAAAGAAAGATGGTTCATAATAATTT
ATAAGCATTTATGTGATAGT

Product: PTS system transporter subunit IID

Products: protein histidine; sugar phosphate; D-glucosamine-6-phosphate [Cytoplasm]; pyruvate; glucose-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EII-M-Man; EIID-Man; PTS system mannose-specific EIID component [H]

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNIAYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTP
HISTLLVGISGAMEEENAKNKEFDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLSVNVGSGEWAEPIQTYLDQIMPC
MLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAAIGVV

Sequences:

>Translated_280_residues
MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNIAYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTP
HISTLLVGISGAMEEENAKNKEFDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLSVNVGSGEWAEPIQTYLDQIMPC
MLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAAIGVV
>Mature_279_residues
TTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNIAYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPH
ISTLLVGISGAMEEENAKNKEFDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINVP
HFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLSVNVGSGEWAEPIQTYLDQIMPCM
LPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAAIGVV

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3716

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IID

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIID domain [H]

Homologues:

Organism=Escherichia coli, GI1788122, Length=275, Percent_Identity=35.6363636363636, Blast_Score=197, Evalue=5e-52,
Organism=Escherichia coli, GI1789529, Length=269, Percent_Identity=34.9442379182156, Blast_Score=153, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004704
- InterPro:   IPR018405 [H]

Pfam domain/function: PF03613 EIID-AGA [H]

EC number: NA

Molecular weight: Translated: 30980; Mature: 30849

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: PS51108 PTS_EIID

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
6.4 %Met     (Translated Protein)
7.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
6.1 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNIAYAYMMAPIIRRLYKD
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
DKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKEFDANSINAVKSSLMGPVS
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHH
GIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINVPHFIIRYICLDKGFKYGTQF
HCCCHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
FKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLSVNVGSGEWAEPIQTYLDQIMPC
HHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHH
MLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAAIGVV
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNIAYAYMMAPIIRRLYKD
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
DKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKEFDANSINAVKSSLMGPVS
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHH
GIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINVPHFIIRYICLDKGFKYGTQF
HCCCHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
FKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLSVNVGSGEWAEPIQTYLDQIMPC
HHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHH
MLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAAIGVV
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N p -phosphohistidine; sugar; phosphoenolpyruvate; glucosamine [Periplasm]; phosphoenolpyruvate; beta-D-glucose [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; fructose [Periplasm] [C]

Specific reaction: protein N p -phosphohistidine + sugar = protein histidine + sugar phosphate phosphoenolpyruvate + glucosamine [Periplasm] = D-glucosamine-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm]

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]