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The map label for this gene is dsaVM [H]

Identifier: 126698504

GI number: 126698504

Start: 1102804

End: 1103877

Strand: Direct

Name: dsaVM [H]

Synonym: CD0927

Alternate gene names: 126698504

Gene position: 1102804-1103877 (Clockwise)

Preceding gene: 126698503

Following gene: 126698505

Centisome position: 25.7

GC content: 34.45

Gene sequence:

>1074_bases
TTGCTTACATTTTTAGATTTATTCGCAGGGATAGGTGGCTTTAGGCTAGGGATGGAAAAAGCAGGACATAAATGTTTGGG
ACATTGCGAATATGATAAATTCGCAAATTTAAGTTATAATGCCATGCACAAACCGAAGGAGGATGAATGGTTTGAAAGAG
ATATTAGAGAAATTAGAACAGAAAATATCCCAAGAGCAGATGTCTGGTGTTTTGGATTCCCATGTCAAGACATTTCTGTT
GCAGGGAAACAATTTGGATTCAGAGGAGAACGTTCAAGTTTATTTTTTACAGTTACAAAACTTATTAGAGAACTCAAAGA
AGAAGATAGACCCAAGTATTTACTTATTGAAAACGTTAAAAATCTACTTAGTGTTAATGGAGGATTTGATTTCCTCAAAG
TTCTCGTTGAACTGGATGAAATCGGCTATGATGCAGAGTGGCAAGTTCTTAATTCTAAAAACTTCGGAGTACCCCAAAAT
AGAGAACGAATATTCATTGTTGGACATTTTAGAGGACGAAGTACACGAAAAGTATTTCCTATCGAAAGAAAAAGTAGAAA
AAATCTTGAGCAACTAAATAATCCAACTCATAGTACAAATAGAATTTATGATGCAGTTGGAATTGCTAGATGTATTAGAA
GTCAGGCAGGAGGTGGAGGTGCTAAAACAGGTCTATACTTTATAGACTTAAATAAAAACTCTAAAGTAACAATAAATGCT
AGATGCCTTAAAGCAAAATATAATGCAGGTGTGACAAATAGAAATTGTGATAATAGTGGAGTTTTAGTTAATGCAGTTTT
AACGCCCGATAGGGTAAATAAAAGACAAAATGGTCGTAGAATTAAAGAAAGCGGAGAAACAATGTTCACATTGACAGCTC
AAGATAAACATGGAATTTTGAAAAATGGAGATATAAGAAGGTTAACACCAAAGGAATGCTTTAGGTTGCAAGGATTTCCG
GATAAATATTACGAAAGAGCAGCAAGTGTATGCTCAGATAGTCAACTGTACAAGCAAGCAGGAAATGCTGTTACTGCAAA
TGTTGTATATGAAATAGCAAAAAGAATGGGCTAA

Upstream 100 bases:

>100_bases
TGTAATTAAAACAGTTTAGAGAGTTGCAAAATATCTTTTGGTATAAATTATTGTTGAAGTGTTTTGTGACTCTCAAAAAT
GAAAATAAGGAGGCGTTGTA

Downstream 100 bases:

>100_bases
AAGTTGCAAAATGTCTTTTAGTATGAATATTTTTGAAGTGTTTTGTAACTCTCAAAAATGAAAATAAGGGGTGGGATAAA
TGTATGAATATATATTAAGA

Product: DNA methylase

Products: NA

Alternate protein names: M.DsaV; Cytosine-specific methyltransferase DsaV [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MLTFLDLFAGIGGFRLGMEKAGHKCLGHCEYDKFANLSYNAMHKPKEDEWFERDIREIRTENIPRADVWCFGFPCQDISV
AGKQFGFRGERSSLFFTVTKLIRELKEEDRPKYLLIENVKNLLSVNGGFDFLKVLVELDEIGYDAEWQVLNSKNFGVPQN
RERIFIVGHFRGRSTRKVFPIERKSRKNLEQLNNPTHSTNRIYDAVGIARCIRSQAGGGGAKTGLYFIDLNKNSKVTINA
RCLKAKYNAGVTNRNCDNSGVLVNAVLTPDRVNKRQNGRRIKESGETMFTLTAQDKHGILKNGDIRRLTPKECFRLQGFP
DKYYERAASVCSDSQLYKQAGNAVTANVVYEIAKRMG

Sequences:

>Translated_357_residues
MLTFLDLFAGIGGFRLGMEKAGHKCLGHCEYDKFANLSYNAMHKPKEDEWFERDIREIRTENIPRADVWCFGFPCQDISV
AGKQFGFRGERSSLFFTVTKLIRELKEEDRPKYLLIENVKNLLSVNGGFDFLKVLVELDEIGYDAEWQVLNSKNFGVPQN
RERIFIVGHFRGRSTRKVFPIERKSRKNLEQLNNPTHSTNRIYDAVGIARCIRSQAGGGGAKTGLYFIDLNKNSKVTINA
RCLKAKYNAGVTNRNCDNSGVLVNAVLTPDRVNKRQNGRRIKESGETMFTLTAQDKHGILKNGDIRRLTPKECFRLQGFP
DKYYERAASVCSDSQLYKQAGNAVTANVVYEIAKRMG
>Mature_357_residues
MLTFLDLFAGIGGFRLGMEKAGHKCLGHCEYDKFANLSYNAMHKPKEDEWFERDIREIRTENIPRADVWCFGFPCQDISV
AGKQFGFRGERSSLFFTVTKLIRELKEEDRPKYLLIENVKNLLSVNGGFDFLKVLVELDEIGYDAEWQVLNSKNFGVPQN
RERIFIVGHFRGRSTRKVFPIERKSRKNLEQLNNPTHSTNRIYDAVGIARCIRSQAGGGGAKTGLYFIDLNKNSKVTINA
RCLKAKYNAGVTNRNCDNSGVLVNAVLTPDRVNKRQNGRRIKESGETMFTLTAQDKHGILKNGDIRRLTPKECFRLQGFP
DKYYERAASVCSDSQLYKQAGNAVTANVVYEIAKRMG

Specific function: This methylase recognizes the double-stranded sequence CCNGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the DsaV endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=389, Percent_Identity=30.3341902313625, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 40602; Mature: 40602

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTFLDLFAGIGGFRLGMEKAGHKCLGHCEYDKFANLSYNAMHKPKEDEWFERDIREIRT
CCHHHHHHHCCCHHHHCHHHHCCHHCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
ENIPRADVWCFGFPCQDISVAGKQFGFRGERSSLFFTVTKLIRELKEEDRPKYLLIENVK
CCCCCCCEEEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHH
NLLSVNGGFDFLKVLVELDEIGYDAEWQVLNSKNFGVPQNRERIFIVGHFRGRSTRKVFP
HHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCCCEECC
IERKSRKNLEQLNNPTHSTNRIYDAVGIARCIRSQAGGGGAKTGLYFIDLNKNSKVTINA
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCEEEEEE
RCLKAKYNAGVTNRNCDNSGVLVNAVLTPDRVNKRQNGRRIKESGETMFTLTAQDKHGIL
EEEEEECCCCCCCCCCCCCCEEEEEEECCHHHCHHHCCCHHHHCCCEEEEEEECCCCCCC
KNGDIRRLTPKECFRLQGFPDKYYERAASVCSDSQLYKQAGNAVTANVVYEIAKRMG
CCCCCCCCCHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHCCCEEEHHHHHHHHHHCC
>Mature Secondary Structure
MLTFLDLFAGIGGFRLGMEKAGHKCLGHCEYDKFANLSYNAMHKPKEDEWFERDIREIRT
CCHHHHHHHCCCHHHHCHHHHCCHHCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
ENIPRADVWCFGFPCQDISVAGKQFGFRGERSSLFFTVTKLIRELKEEDRPKYLLIENVK
CCCCCCCEEEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHH
NLLSVNGGFDFLKVLVELDEIGYDAEWQVLNSKNFGVPQNRERIFIVGHFRGRSTRKVFP
HHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCCCEECC
IERKSRKNLEQLNNPTHSTNRIYDAVGIARCIRSQAGGGGAKTGLYFIDLNKNSKVTINA
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCEEEEEE
RCLKAKYNAGVTNRNCDNSGVLVNAVLTPDRVNKRQNGRRIKESGETMFTLTAQDKHGIL
EEEEEECCCCCCCCCCCCCCEEEEEEECCHHHCHHHCCCHHHHCCCEEEEEEECCCCCCC
KNGDIRRLTPKECFRLQGFPDKYYERAASVCSDSQLYKQAGNAVTANVVYEIAKRMG
CCCCCCCCCHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHCCCEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7971279 [H]