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The map label for this gene is yqhM [H]

Identifier: 126698313

GI number: 126698313

Start: 896589

End: 897404

Strand: Direct

Name: yqhM [H]

Synonym: CD0733

Alternate gene names: 126698313

Gene position: 896589-897404 (Clockwise)

Preceding gene: 126698312

Following gene: 126698314

Centisome position: 20.9

GC content: 29.78

Gene sequence:

>816_bases
ATGAATCAGTGGAGAGTTATACATAATAAATCATATGAAGGAGCAATGAATATGGCAATTGACGAAGCTATATTTACTGC
ATATAAGAAGGGACATAATAAGCCAACTCTTAGATTTTATACATGGGAACCAGCTTGTTTGAGTATAGGATATTTTCAGA
AATTGGAAGATGAGATAGATTTAGATAAGTGTAGATGTATGAATATAGACTATACTAGAAGAATTACAGGTGGAAGAGCT
GTATTACATGATAATGAGCTAACCTATAGTATAATTATAGGAGAAGATAATCCTTTAATCGATAAGAGCATCAACCTATC
TTATAGATATATAAGTGAAGGTCTAGTTAAAGGATTGAATCTAAGTGGTATAGAAACAGATAATTTAAATAGAGGCGAGA
GAATAAGTAGGGAAAATTTATCAGCTGCATGTTTTAATGCACATGCATCATATGAAGTTACTATTAATAATAAGAAGGTA
ATTGGAAGTGCTCAAAGCAGAAAAGATGGTGTTTTATTACAACATGGTTCAATAATACTGGATTTTGATGTAGAAAAATT
GTTTAAATTAATAAAAACTAAGACACCAGAATTGAAAGAAAGAGCTATGAAATTTACAGCTAAAAAAGCAAGTGGAATAG
AAAATGAAATTGGCAGAAAAATAGACATAGATATTCTACAGAAAAACATAGTAAAGGGATTAGCTGAACAATTTAATGTT
GAGTTTGTGGAGGGCGATTTGACTGATTATGAGAAGCAATTAGTTAAAGAACTATATGAAAAATACAAGAATGAAGAGTA
TAATAAAAAGCGTTAA

Upstream 100 bases:

>100_bases
AGACCTTTAAATCAAAGTGAGATAGATTTAGCAATAAGAGAAATAAATCTATAGAGGATTTCTAGGATATATTTATACTA
AAAATGGGGGCGTAATAATC

Downstream 100 bases:

>100_bases
CTTTAATTGACTTTACATAAATTAAAGTGATATACTTAAGGTATAATAATATACAAAAACTGAAATCATGAAGGTTTCTT
TGGTGTATTGAAATACCTAA

Product: biotin/lipoate-protein ligase

Products: lipoyl-AMP; pyrophosphate; N6-lipoyl-lysine [C]

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEPACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRA
VLHDNELTYSIIIGEDNPLIDKSINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIGRKIDIDILQKNIVKGLAEQFNV
EFVEGDLTDYEKQLVKELYEKYKNEEYNKKR

Sequences:

>Translated_271_residues
MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEPACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRA
VLHDNELTYSIIIGEDNPLIDKSINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIGRKIDIDILQKNIVKGLAEQFNV
EFVEGDLTDYEKQLVKELYEKYKNEEYNKKR
>Mature_271_residues
MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEPACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRA
VLHDNELTYSIIIGEDNPLIDKSINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIGRKIDIDILQKNIVKGLAEQFNV
EFVEGDLTDYEKQLVKELYEKYKNEEYNKKR

Specific function: Catalyzes Both The ATP-Dependent Activation Of Exogenously Supplied Lipoate To Lipoyl-Amp And The Transfer Of The Activated Lipoyl On The Lipoate-Dependent Enzymes. Creates An Amide Linkage That Joins The Free Carboxyl Group Of Lipoic Acid To The Epsilon-

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 31220; Mature: 31220

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEPACLSIGYFQKLEDEID
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHCC
LDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDKSINLSYRYISEGLVKGLN
CCHHEECCCCHHEEECCCEEEEECCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCC
LSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKVIGSAQSRKDGVLLQHGSIIL
CCCCCCCCCCCCCHHCHHCCCEEEEECCCEEEEEECCCEEECCCCCCCCCEEEECCCEEE
DFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIGRKIDIDILQKNIVKGLAEQFNV
EECHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHCCEEEHHHHHHHHHHHHHHHCCE
EFVEGDLTDYEKQLVKELYEKYKNEEYNKKR
EEEECCCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEPACLSIGYFQKLEDEID
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHCC
LDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDKSINLSYRYISEGLVKGLN
CCHHEECCCCHHEEECCCEEEEECCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCC
LSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKVIGSAQSRKDGVLLQHGSIIL
CCCCCCCCCCCCCHHCHHCCCEEEEECCCEEEEEECCCEEECCCCCCCCCEEEECCCEEE
DFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIGRKIDIDILQKNIVKGLAEQFNV
EECHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHCCEEEHHHHHHHHHHHHHHHCCE
EFVEGDLTDYEKQLVKELYEKYKNEEYNKKR
EEEECCCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lipoic acid; ATP [C]

Specific reaction: Catalyzes first the reaction of lipoic acid and ATP to form lipoyl-AMP and pyrophosphate, then the formation N6-lipoyl-lysine from a specific lysine residue in lipoate-dependent enzymes [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Other Carbon-Nitrogen Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]