Definition Clostridium difficile 630 chromosome, complete genome.
Accession NC_009089
Length 4,290,252

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The map label for this gene is argS

Identifier: 126698290

GI number: 126698290

Start: 868299

End: 869999

Strand: Direct

Name: argS

Synonym: CD0711

Alternate gene names: 126698290

Gene position: 868299-869999 (Clockwise)

Preceding gene: 126698289

Following gene: 126698291

Centisome position: 20.24

GC content: 31.57

Gene sequence:

>1701_bases
ATGCAAGATTTTAAGGTGGCAATATCAAATTGCCTTAAAGAAAAAATAGAAGATTTATCGAAAGAAGAGATAGAGGCACT
TATAGAAGTTCCACCAAATAAGGATATGGGGGACTATGCTTTTCCATGCTTTAAATTAGCCAAAGTGTTTAGAAAAGCTC
CAAACATGATAGCAAGTGAATTGGCGGAAAGTATAGAACCAAGTGGAGAGATAACAAAAGTAATACAATTAGGCGGATAT
GTAAATTTCTTTGTAAATAAATCTCAATTAGCAGAAACTGTAATTAAAAAAGTTTTAGACGAAAAAGAAAATTACGGACA
TAGTGATTTTGGAAAAGATAAAACTGTAATAGTTGAATATTCTTCTCCAAATATAGCAAAACCTTTCCATATAGGGCACA
TAAGAACTACAGTTATAGGTAATGCTTTATATAAGATATATGATTCGCAAGGATACAAGACTATAAGAATAAATCATTTA
GGTGACTATGGAACTCAGTTTGGTAAGTTAATAGTGGCCTTTAAAAAGTGGGGAGAAAAAGAAGTAGTAGAGTCTAATCC
AATTCCAGAATTGCTAAAATTATACGTAAGATTCCATGATGAAGCTGAACAACACCCAGAAATGGAAGATGAAGCAAGAG
CATGGTTTAATAAATTAGAAAATGGTGACGAAGAAGCACAAGAATTATGGCAATGGTTTAGAAATGAGAGTTTAAAAGAG
TTTAACAGAGTTTATAAACTTTTAGATATAGAATTTGATTCTTTAGCAGGAGAGAGTTTTTATTCTGATAAAATGAACAG
AGTTATCGAACTATTAGAAGAGAAAAATCTTTTAAAAGAGTCTAAAGGAGCAAGAATTGTTGATTTAGAAGAGTATAAAA
TGCCACCAGCACTTATTACTAAGAATGATGGTTCAACGTTATATATGACAAGAGATTTAGCTGCTGCAATATATAGAAAA
GAAACTTATGATTTTGACAAGTGTATATATGTAGTTGGTTCTCAACAAAACTTACATTTCCAACAATGGTTTAAGGTAAT
TGAGCTTATGGGATATGATTGGGCAAAAGATTTAATTCATGTTGGATTTGGTATGGTTGCACTTGAAGAAGGTACTATGT
CGACTAGAAAAGGAAGAGTAGTATTTTTAGAAGATGCTTTAAATCAAGCTATAGATAAAACTAAGGAAATTATACTAGCT
AAAAACCCAAATGCTAAAAATGTGGATGAAATATCTAAGCAAGTAGGTGTTGGGGCAGTAGTATTCCAAGAGTTATCTAA
TAGTAGAATTAAAGACTATACCTTCTCTTGGGAAAGAACGCTTAGTTTTGATGGTGAGACTGGACCATATGTTCAGTATA
CACATGCTAGATGCTGTGCAGTTTTAAGAAAAGCAGAAGTAGAAGTGACTTCTGATATAGATTATAGTCTATTAGCTGAT
GAAGATAGTGCAGAAGTTCTTAGAGTTATAGAATCATTCAATAAGAATATTTTACTTGCTCTAAAGAAAAATGAGCCACA
TATAGTGACTAGATTTATGCTAGATTTAGCTCAAGCGTTTAATAAATTTTATCATGACAATCCAATTTTAGTAGAAAATT
TGGAAATAAGAAAAGCAAGACTTGCATTAGTTTTAGCAACAAAACAAACTTTAGAAAATTCTCTGAAATTGTTGGGAATG
CACGCTCCTGAAAGAATGTAG

Upstream 100 bases:

>100_bases
TTTACACTGTAATGAAATACTTGAAAAAACACGAAATTTGATTTAACATAAAGAGATAAGCACAATATAAACCAGGAACA
ATGTAGAGAGGAGCAACAGA

Downstream 100 bases:

>100_bases
CATGATAAAATGTTGAAATAAAAAGAATAAAATTGAACTTAGTACGCAATTTGTATAACTGTAAAATATAGGGATGTCGA
AAACTGATGCTTTACATATA

Product: arginyl-tRNA synthetase

Products: NA

Alternate protein names: Arginine--tRNA ligase; ArgRS

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCFKLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGY
VNFFVNKSQLAETVIKKVLDEKENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNKLENGDEEAQELWQWFRNESLKE
FNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKNLLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRK
ETYDFDKCIYVVGSQQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAIDKTKEIILA
KNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPYVQYTHARCCAVLRKAEVEVTSDIDYSLLAD
EDSAEVLRVIESFNKNILLALKKNEPHIVTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGM
HAPERM

Sequences:

>Translated_566_residues
MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCFKLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGY
VNFFVNKSQLAETVIKKVLDEKENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNKLENGDEEAQELWQWFRNESLKE
FNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKNLLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRK
ETYDFDKCIYVVGSQQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAIDKTKEIILA
KNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPYVQYTHARCCAVLRKAEVEVTSDIDYSLLAD
EDSAEVLRVIESFNKNILLALKKNEPHIVTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGM
HAPERM
>Mature_566_residues
MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCFKLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGY
VNFFVNKSQLAETVIKKVLDEKENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNKLENGDEEAQELWQWFRNESLKE
FNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKNLLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRK
ETYDFDKCIYVVGSQQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAIDKTKEIILA
KNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPYVQYTHARCCAVLRKAEVEVTSDIDYSLLAD
EDSAEVLRVIESFNKNILLALKKNEPHIVTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGM
HAPERM

Specific function: Unknown

COG id: COG0018

COG function: function code J; Arginyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI197100773, Length=526, Percent_Identity=37.2623574144487, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI15149476, Length=531, Percent_Identity=29.3785310734463, Blast_Score=215, Evalue=1e-55,
Organism=Escherichia coli, GI1788184, Length=549, Percent_Identity=33.879781420765, Blast_Score=303, Evalue=3e-83,
Organism=Caenorhabditis elegans, GI71985061, Length=588, Percent_Identity=28.5714285714286, Blast_Score=204, Evalue=8e-53,
Organism=Caenorhabditis elegans, GI71985068, Length=552, Percent_Identity=28.6231884057971, Blast_Score=192, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI17532153, Length=498, Percent_Identity=26.5060240963855, Blast_Score=159, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6320548, Length=570, Percent_Identity=38.5964912280702, Blast_Score=393, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6321883, Length=580, Percent_Identity=35.5172413793103, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI28571570, Length=490, Percent_Identity=34.2857142857143, Blast_Score=254, Evalue=8e-68,
Organism=Drosophila melanogaster, GI18859963, Length=538, Percent_Identity=29.9256505576208, Blast_Score=215, Evalue=6e-56,

Paralogues:

None

Copy number: 789 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYR_CLOD6 (Q189Q7)

Other databases:

- EMBL:   AM180355
- RefSeq:   YP_001087187.1
- ProteinModelPortal:   Q189Q7
- STRING:   Q189Q7
- GeneID:   4916819
- GenomeReviews:   AM180355_GR
- KEGG:   cdf:CD0711
- NMPDR:   fig|1496.1.peg.1940
- eggNOG:   COG0018
- HOGENOM:   HBG695395
- OMA:   YNDDLQP
- ProtClustDB:   PRK01611
- GO:   GO:0005737
- HAMAP:   MF_00123
- InterPro:   IPR001412
- InterPro:   IPR001278
- InterPro:   IPR015945
- InterPro:   IPR005148
- InterPro:   IPR008909
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.30.1360.70
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11956
- PRINTS:   PR01038
- SMART:   SM00836
- TIGRFAMs:   TIGR00456

Pfam domain/function: PF03485 Arg_tRNA_synt_N; PF05746 DALR_1; PF00750 tRNA-synt_1d; SSF55190 Arg-tRNA-synth_Ic_N; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.19

Molecular weight: Translated: 64991; Mature: 64991

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCFKLAKVFRKAPNMIASE
CCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHH
LAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEKENYGHSDFGKDKTVIVEY
HHHHCCCCCHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEE
SSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHLGDYGTQFGKLIVAFKKWGEK
CCCCCCCCEEECHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCC
EVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNKLENGDEEAQELWQWFRNESLKE
HHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHH
FNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKNLLKESKGARIVDLEEYKMPPALIT
HHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCCEEE
KNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGSQQNLHFQQWFKVIELMGYDWAKDLIH
CCCCCEEEEHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHH
VGFGMVALEEGTMSTRKGRVVFLEDALNQAIDKTKEIILAKNPNAKNVDEISKQVGVGAV
HCCCCEEEECCCCCCCCCCEEEHHHHHHHHHHHHHCEEEECCCCCCCHHHHHHHHCCHHH
VFQELSNSRIKDYTFSWERTLSFDGETGPYVQYTHARCCAVLRKAEVEVTSDIDYSLLAD
HHHHHHCCCCCCEEECEEEEEECCCCCCCEEEEHHHHHHHHHHHHCEEEECCCCEEEECC
EDSAEVLRVIESFNKNILLALKKNEPHIVTRFMLDLAQAFNKFYHDNPILVENLEIRKAR
CCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHH
LALVLATKQTLENSLKLLGMHAPERM
HHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCFKLAKVFRKAPNMIASE
CCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHH
LAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEKENYGHSDFGKDKTVIVEY
HHHHCCCCCHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEE
SSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHLGDYGTQFGKLIVAFKKWGEK
CCCCCCCCEEECHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCC
EVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNKLENGDEEAQELWQWFRNESLKE
HHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHH
FNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKNLLKESKGARIVDLEEYKMPPALIT
HHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCCEEE
KNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGSQQNLHFQQWFKVIELMGYDWAKDLIH
CCCCCEEEEHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHH
VGFGMVALEEGTMSTRKGRVVFLEDALNQAIDKTKEIILAKNPNAKNVDEISKQVGVGAV
HCCCCEEEECCCCCCCCCCEEEHHHHHHHHHHHHHCEEEECCCCCCCHHHHHHHHCCHHH
VFQELSNSRIKDYTFSWERTLSFDGETGPYVQYTHARCCAVLRKAEVEVTSDIDYSLLAD
HHHHHHCCCCCCEEECEEEEEECCCCCCCEEEEHHHHHHHHHHHHCEEEECCCCEEEECC
EDSAEVLRVIESFNKNILLALKKNEPHIVTRFMLDLAQAFNKFYHDNPILVENLEIRKAR
CCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHH
LALVLATKQTLENSLKLLGMHAPERM
HHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA