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The map label for this gene is ogt1 [H]

Identifier: 126698179

GI number: 126698179

Start: 723491

End: 724024

Strand: Direct

Name: ogt1 [H]

Synonym: CD0601

Alternate gene names: 126698179

Gene position: 723491-724024 (Clockwise)

Preceding gene: 126698176

Following gene: 126698181

Centisome position: 16.86

GC content: 32.58

Gene sequence:

>534_bases
ATGCAATATATTAGCTATTACCATAGTCCAATTGGAAATATTCTTTTAGCATCAGATAATATTGGGTTAACTGGTCTTTG
GTTTGAGAATCAAAAGTATTATGCTTATAATCTTGACCAAGAACATCAAGAAAAAGAATTGCCAATTTTCGAGCAGACAA
AGAAATGGTTAGATATTTATTTTTCGGGAAAAGAACCTAATTTTACACCATCACTCCATATGGTAGGTACTCCTTTTCAG
ACTACAGTGTGGAAAATTTTACAACAAATACCATATGGTAAAACAATCACTTATGGAGAAATTGCATATAAAGTTGCTCA
ACAAAAAGGAATATCTAAAATGTCAGCACAAGCAATCGGTGGTGCAGTAGGGCATAATGGAATCTCTATTATTATTCCAT
GTCATCGTGTTGTAGGAACAAATGGGAGTTTGACTGGGTATGCTGGTGGAATTGATAGAAAAATAAAATTGTTATCTTTA
GAAAAAGTATCTGTGCAACATTATTTTATTCCAAAGAAAGGGAGCGCATTATAA

Upstream 100 bases:

>100_bases
GTTAGAAGGAAATTATATTAGTATGTTTTTACATAATGGTAGATAGAAAAGAAGTGTCTAATGATTTGAGTGAAGTTTTC
AGAATATAAGGAGGAATTGT

Downstream 100 bases:

>100_bases
AGCCATTAAATTTAGAAATTCTTAATCATTTATTTGATATACAAACATCTAATTTTGAAAGCAATAACTTGCAATTTTAA
AAAATGATTATTTAAACTAT

Product: methylated-DNA--protein-cysteine methyltransferase 1

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQEKELPIFEQTKKWLDIYFSGKEPNFTPSLHMVGTPFQ
TTVWKILQQIPYGKTITYGEIAYKVAQQKGISKMSAQAIGGAVGHNGISIIIPCHRVVGTNGSLTGYAGGIDRKIKLLSL
EKVSVQHYFIPKKGSAL

Sequences:

>Translated_177_residues
MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQEKELPIFEQTKKWLDIYFSGKEPNFTPSLHMVGTPFQ
TTVWKILQQIPYGKTITYGEIAYKVAQQKGISKMSAQAIGGAVGHNGISIIIPCHRVVGTNGSLTGYAGGIDRKIKLLSL
EKVSVQHYFIPKKGSAL
>Mature_177_residues
MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQEKELPIFEQTKKWLDIYFSGKEPNFTPSLHMVGTPFQ
TTVWKILQQIPYGKTITYGEIAYKVAQQKGISKMSAQAIGGAVGHNGISIIIPCHRVVGTNGSLTGYAGGIDRKIKLLSL
EKVSVQHYFIPKKGSAL

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=119, Percent_Identity=35.2941176470588, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI1787596, Length=86, Percent_Identity=50, Blast_Score=91, Evalue=4e-20,
Organism=Escherichia coli, GI1788542, Length=113, Percent_Identity=35.3982300884956, Blast_Score=84, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI115533070, Length=91, Percent_Identity=35.1648351648352, Blast_Score=69, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI115533068, Length=91, Percent_Identity=35.1648351648352, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320001, Length=93, Percent_Identity=37.6344086021505, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19829; Mature: 19829

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQEKELPIFEQTKKWLDIY
CCCHHHHCCCCCCEEEECCCCCEEEEEEECCEEEEECCCCHHHHCCCCHHHHHHCEEEEE
FSGKEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIAYKVAQQKGISKMSAQAIG
ECCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCCHHHHHHHHC
GAVGHNGISIIIPCHRVVGTNGSLTGYAGGIDRKIKLLSLEKVSVQHYFIPKKGSAL
CCCCCCCEEEEEECHHEECCCCCEEEECCCCCCEEEEEEEEEEEEEEEEECCCCCCC
>Mature Secondary Structure
MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQEKELPIFEQTKKWLDIY
CCCHHHHCCCCCCEEEECCCCCEEEEEEECCEEEEECCCCHHHHCCCCHHHHHHCEEEEE
FSGKEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIAYKVAQQKGISKMSAQAIG
ECCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCCHHHHHHHHC
GAVGHNGISIIIPCHRVVGTNGSLTGYAGGIDRKIKLLSLEKVSVQHYFIPKKGSAL
CCCCCCCEEEEEECHHEECCCCCEEEECCCCCCEEEEEEEEEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]