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The map label for this gene is pleD [H]

Identifier: 126698116

GI number: 126698116

Start: 641051

End: 642010

Strand: Direct

Name: pleD [H]

Synonym: CD0537

Alternate gene names: 126698116

Gene position: 641051-642010 (Clockwise)

Preceding gene: 126698115

Following gene: 126698117

Centisome position: 14.94

GC content: 29.38

Gene sequence:

>960_bases
ATGAAAAATAAGCAACAGGGAACAATTTTAGTCGTTGATGATAGTGTTCTGATGTGTGACTTAATAGATAAAACACTTAC
AAAAGATAATTTTAAGGTTAAAAAAGCATTTAATGCAATAGAGGCAAAAGAATTTATTGAAGAATTTATACCAGACATTA
TTTTGCTTGATATAATTTTACCAGATAATTCTGGTTTTGAGTTTTGTAAGGAAGTAAAATCTAATGCTCGTACAGCAGAT
ATACCTATAATTTTTATAACTTCAAAAAATACAGATACAGACATAGTAAAAGGATTTGGTGTAGGAGCAATTGACTATAT
GGTGAAACCTTTTAGTATGACAGAATTAAAAGCTCGTGTTATAGCTCATCTTGAAGTAAAGAAATCTCAAGATAAACTAA
AAAAGATAAATAATGAACTGGAATCTTCTCTTGAAAAATTGAATAGACTCGTTGTAAGAGATTATCTTACAGGTCTTTAC
AATAGGCGACATATAATTAATCAGCTTATGGAGCAAAGAAGAATTAATAAATATAGTGAAACATCAATATCCCTTATCCT
TGGTGATATTGATGACTTTAAGGTTATCAATGATACTTATGGGCATGAAGCAGGGGATTTCGTGTTAACAGTAATATCAA
GTATAATAAAAAAGAATTGTAGACAAATTGATACTGTATCAAGATGGGGTGGAGAAGAGTTTTTGATAATGCTCACAAAT
ACTCCAATAGAAGGTGCTAAGATACTTTCAGAAAGAATCCGTAAAGAGATAAAGGAGTTTGATTTCAACTATAAAGAACA
TAAAATAAAGTGCACTATTACATTAGGAGTAGTAGAAGTAAACTCAAATATATCATTAGAAGAAAACATATCCTTAGCAG
ATAAAGCAATGTATGAGGGTAAAAACTTAGGAAAAGATTGTAGCGTAGTGAGTACAATGAAAGGATTAAAAAAGATATAA

Upstream 100 bases:

>100_bases
TTGTGATGAGAACATTTAAGAATATAGTGGTATAATTATTAAGTATAAAAATTTTAAATCATTAAAATTTGAATATATTA
TGTTTAAGAGAGGTTTGTAT

Downstream 100 bases:

>100_bases
ACTTACTTTATGAAGGAGTACAGATATGAAAAGAGAAGTAAAGCTTTCTAAATTAGCAATTGTATTGCTGATTATTATAG
TATTGGCTGTTCTAGCAGGG

Product: chemosensory protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKEFIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTAD
IPIIFITSKNTDTDIVKGFGVGAIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKKNCRQIDTVSRWGGEEFLIMLTN
TPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLGVVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI

Sequences:

>Translated_319_residues
MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKEFIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTAD
IPIIFITSKNTDTDIVKGFGVGAIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKKNCRQIDTVSRWGGEEFLIMLTN
TPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLGVVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI
>Mature_319_residues
MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKEFIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTAD
IPIIFITSKNTDTDIVKGFGVGAIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKKNCRQIDTVSRWGGEEFLIMLTN
TPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLGVVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=172, Percent_Identity=37.7906976744186, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI87081881, Length=163, Percent_Identity=34.9693251533742, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1786584, Length=173, Percent_Identity=30.635838150289, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1787816, Length=153, Percent_Identity=36.6013071895425, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI87082007, Length=170, Percent_Identity=33.5294117647059, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786599, Length=114, Percent_Identity=38.5964912280702, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI145693134, Length=154, Percent_Identity=32.4675324675325, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788381, Length=169, Percent_Identity=29.585798816568, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081974, Length=159, Percent_Identity=32.0754716981132, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789809, Length=116, Percent_Identity=34.4827586206897, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787802, Length=168, Percent_Identity=32.7380952380952, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1788085, Length=169, Percent_Identity=33.1360946745562, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1788394, Length=131, Percent_Identity=32.0610687022901, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790860, Length=116, Percent_Identity=32.7586206896552, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790552, Length=136, Percent_Identity=32.3529411764706, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787375, Length=114, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87081977, Length=155, Percent_Identity=30.9677419354839, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786784, Length=112, Percent_Identity=35.7142857142857, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788956, Length=177, Percent_Identity=31.638418079096, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787541, Length=162, Percent_Identity=29.6296296296296, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790299, Length=256, Percent_Identity=23.4375, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788550, Length=138, Percent_Identity=27.536231884058, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1790863, Length=116, Percent_Identity=26.7241379310345, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 36299; Mature: 36299

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKEFIEEFIPDIILLDIIL
CCCCCCCEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEE
PDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVGAIDYMVKPFSMTELKARV
CCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHCCCCHHHHHCCCCCHHHHHHHH
IAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLYNRRHIINQLMEQRRINKYSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKKNCRQIDTVSRWGGEEFLIMLTN
CEEEEEEECCCCCEEECHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
TPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLGVVEVNSNISLEENISLADKAMYEG
CCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECCCCCCCCCCCHHHHHHHCC
KNLGKDCSVVSTMKGLKKI
CCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKEFIEEFIPDIILLDIIL
CCCCCCCEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEE
PDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVGAIDYMVKPFSMTELKARV
CCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHCCCCHHHHHCCCCCHHHHHHHH
IAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLYNRRHIINQLMEQRRINKYSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKKNCRQIDTVSRWGGEEFLIMLTN
CEEEEEEECCCCCEEECHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
TPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLGVVEVNSNISLEENISLADKAMYEG
CCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECCCCCCCCCCCHHHHHHHCC
KNLGKDCSVVSTMKGLKKI
CCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA