Click here to switch to the map view.

The map label for this gene is orf20 [H]

Identifier: 126698076

GI number: 126698076

Start: 588010

End: 589218

Strand: Direct

Name: orf20 [H]

Synonym: CD0499

Alternate gene names: 126698076

Gene position: 588010-589218 (Clockwise)

Preceding gene: 126698075

Following gene: 126698077

Centisome position: 13.71

GC content: 41.11

Gene sequence:

>1209_bases
TTGATTGGAGGAATTTCACTGAATGAACAAACTTGGGTACAGCATTTAAAGGAAAAACGCTTATCTTATGGACTTTCTCA
AAACCGACTGGCTATTGCTACAGGCATTACAAGGCAATATCTTAGCGACATTGAAACTGGCAAGGTCAAGCCATCAGACG
AATTACAACTGGCACTTTTAGAAACGCTGGAACGCTTCAATCCCGACGCTCCCCTTGAAATGCTCTTTGACTATGTAAGG
ATTCGGTTTCCAACAACAGACGTACAGCATGTGGTCGAGGACGTTTTACGATTGAAACTTTCCTATATGCTCCATGAAGA
CTATGGCTTCTATTCCTATACCGAACATTATGCTTTAGGCAATATTTTCGTCCTATGCTCTCATGAGCTGGATAAAGGGG
TTCTAGTGGAATTGAAAGGTCGTGGGTGCAGACAATTTGAAAGCTATCTCTTAGCACAACAAAGAAGCTGGTATGAGTTC
TTTATGGACGCTTTGGTGGCTAGTGGCGTGATGAAACGCCTTGACCTTGCCATCAACGATAAAACAGGGATTTTGAATAT
TCCCATACTGACGGAAAAATGCCGACAGGAAGAGTGTATTTCGGTGTTCCGCAGTTTCAAGAGCTATCGCAGTGGCGAAC
TGGTACGCAAAGATGAAAAGGAATGTATGGGAAACACACTTTATATCGGTTCATTACAAAGCGAAGTCTATTTCTGTATC
TATGAAAAGGACTATGAGCAGTACAAGAAAAATGATATTCCCATTGAAGACGCAGAGGTTAAAAACCGTTTTGAAATCCG
ACTAAAAAATGAGCGTGCCTATTATGCAGTACGTGATTTACTTGCCTATGATAATCCAGAGCATACAGCTTTTAAGATTA
TCAATCGGTATATCCGCTTTGTGGATAAGGACGATTCAAAAGCACGTTCCGATTGGAAACTCAATGAAGAATGGGCATGG
TTTATTGGAAACAATCGTGAACGATTAAAACTAACCACAAAACCAGAGCCTTACTCTTTCCAAAGGACACTAAACTGGCT
ATCTCATCAAGTCGCCCCGACCTTAAAGGTTGCTATTACACTTGATGAAATTAACCAGACGCAGGTTGTGAAAGACATTC
TCGACCATGCGAAACTGACAGACCGACACAAGCAGATTTTAAAGCAACAGTCGGTAAAAGAACAGGACGTGATAACGAAC
AAAAAATAA

Upstream 100 bases:

>100_bases
AGGAAACAGGCAAAAAAATAGTAGAAAATCCTTTGGTTACAAGGGATTTAGAAAATTTCGGTGTATGTCAAATGAGCTTT
AAAAGTTGACATACGCCTTT

Downstream 100 bases:

>100_bases
CTTAAATACAAATTTACTGAATACAGAAAGGAGAACATTTTTATGAATTTTGGACAAAACCTTTATAACTGGTTTCTATC
AAATGCTCAATCACTGGTAC

Product: conjugative transposon replication initiation factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MIGGISLNEQTWVQHLKEKRLSYGLSQNRLAIATGITRQYLSDIETGKVKPSDELQLALLETLERFNPDAPLEMLFDYVR
IRFPTTDVQHVVEDVLRLKLSYMLHEDYGFYSYTEHYALGNIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEF
FMDALVASGVMKRLDLAINDKTGILNIPILTEKCRQEECISVFRSFKSYRSGELVRKDEKECMGNTLYIGSLQSEVYFCI
YEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLLAYDNPEHTAFKIINRYIRFVDKDDSKARSDWKLNEEWAW
FIGNNRERLKLTTKPEPYSFQRTLNWLSHQVAPTLKVAITLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITN
KK

Sequences:

>Translated_402_residues
MIGGISLNEQTWVQHLKEKRLSYGLSQNRLAIATGITRQYLSDIETGKVKPSDELQLALLETLERFNPDAPLEMLFDYVR
IRFPTTDVQHVVEDVLRLKLSYMLHEDYGFYSYTEHYALGNIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEF
FMDALVASGVMKRLDLAINDKTGILNIPILTEKCRQEECISVFRSFKSYRSGELVRKDEKECMGNTLYIGSLQSEVYFCI
YEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLLAYDNPEHTAFKIINRYIRFVDKDDSKARSDWKLNEEWAW
FIGNNRERLKLTTKPEPYSFQRTLNWLSHQVAPTLKVAITLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITN
KK
>Mature_402_residues
MIGGISLNEQTWVQHLKEKRLSYGLSQNRLAIATGITRQYLSDIETGKVKPSDELQLALLETLERFNPDAPLEMLFDYVR
IRFPTTDVQHVVEDVLRLKLSYMLHEDYGFYSYTEHYALGNIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEF
FMDALVASGVMKRLDLAINDKTGILNIPILTEKCRQEECISVFRSFKSYRSGELVRKDEKECMGNTLYIGSLQSEVYFCI
YEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLLAYDNPEHTAFKIINRYIRFVDKDDSKARSDWKLNEEWAW
FIGNNRERLKLTTKPEPYSFQRTLNWLSHQVAPTLKVAITLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITN
KK

Specific function: Probable DNA relaxase involved in the transfer of the integrative and conjugative element ICEBs1. Required for the transfer of ICEBs1. Probably mediates conjugation of ICEBs1 by nicking at oriT on the conjugative element and facilitates the translocation

COG id: COG2946

COG function: function code L; Putative phage replication protein RstA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the plasmid replication initiation factor family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003491 [H]

Pfam domain/function: PF02486 Rep_trans [H]

EC number: NA

Molecular weight: Translated: 47305; Mature: 47305

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGGISLNEQTWVQHLKEKRLSYGLSQNRLAIATGITRQYLSDIETGKVKPSDELQLALL
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHH
ETLERFNPDAPLEMLFDYVRIRFPTTDVQHVVEDVLRLKLSYMLHEDYGFYSYTEHYALG
HHHHHCCCCCCHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHC
NIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFFMDALVASGVMKRLDLAIND
CEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
KTGILNIPILTEKCRQEECISVFRSFKSYRSGELVRKDEKECMGNTLYIGSLQSEVYFCI
CCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHCCCEEEEEEECCCEEEEE
YEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLLAYDNPEHTAFKIINRYIRF
EECCHHHHCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHH
VDKDDSKARSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQVAPTLKVAIT
HCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEE
LDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITNKK
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
>Mature Secondary Structure
MIGGISLNEQTWVQHLKEKRLSYGLSQNRLAIATGITRQYLSDIETGKVKPSDELQLALL
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHH
ETLERFNPDAPLEMLFDYVRIRFPTTDVQHVVEDVLRLKLSYMLHEDYGFYSYTEHYALG
HHHHHCCCCCCHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHC
NIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFFMDALVASGVMKRLDLAIND
CEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
KTGILNIPILTEKCRQEECISVFRSFKSYRSGELVRKDEKECMGNTLYIGSLQSEVYFCI
CCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHCCCEEEEEEECCCEEEEE
YEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLLAYDNPEHTAFKIINRYIRF
EECCHHHHCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHH
VDKDDSKARSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQVAPTLKVAIT
HCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEE
LDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITNKK
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]