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The map label for this gene is spaK [H]

Identifier: 126698059

GI number: 126698059

Start: 570187

End: 571611

Strand: Direct

Name: spaK [H]

Synonym: CD0482

Alternate gene names: 126698059

Gene position: 570187-571611 (Clockwise)

Preceding gene: 126698058

Following gene: 126698065

Centisome position: 13.29

GC content: 32.21

Gene sequence:

>1425_bases
ATGAAAAATCAATCCCTTATTACCCAATTTAGACATACTTTTATTTTTATTATAATAGCCAGTATAGTTGCCACAGTGAT
TACTTATGTATTTGCTCTCTATTTGTATATTCATTCCTTAAATAAGGATATCTATCCACCAAACTATTATGAGCGACAAG
TTCCTAGAATTGAAAAATACATCAATGAAAAAAACATAACTTTGTTATCACAATCGAATGAAGAAGGTTTAAAAAGAACG
ATTCGTGGGGATGATATGCTATATCAAGTTGTGGATAATAATGGCAATATTTTATATGGTACTAACCCCAAAAAACTATT
TAAAACAAAAGAAGAATTATTTAATAATTTTATCAATAAAACTGTTCGTAAAGGTGGTTATATCCATACTGTTCCAATCA
AAGGGGATAATGGAAAGATTGAGGGAGCTGTTATTCTATTCTACCAAGTGAAAATCACCTTTGCAAATATTAGAGGGAGA
TTTGTATTTGCAGTTATTATAATGGCTCTGTTTTCTCCATTTCTATATATCGTGGGTTTTACTAGATGGCTTTCTAAGCG
CTTTGTAAAGAATATCAATCAACCATTACATCTATTGATTGATGCGTCCAAGAAGATAAAAGAAAAGGACCTTGATTTTG
AAATCGACTATTATTCAGATAATGAACTCGGTAAACTTTGTAGTGCATTTTCTGAAATGAAAGACGAATTAAAAGGCTCT
TTATCTGCACAGTGGAAGATGGAGCAGGAACGTGTTGAAATGGTGGAAGCTCTAGCACATGATTTAAAATCTCCTCTTTC
TATTATTTTGGGATATACAGATGCACTTATTGGTAATAATACAGATGACAATGAAAAACTTCATAGGTATCTTACAGTGA
TAAGAGAAAATACAGAAAAAAGTGCTGCTCTTGTCCAAAAGATGCAATATACGTCTGACTTAGAAAAGTCAAATATACAA
TTAAATCTTGTTCCTATAAATTTACCTGAATTTTTGAGGCAAAAGGTGCAAGACTATGAATTACAGGCTCATCAAAAGGA
AGTTGAACTTATATTGAAAATGCAGGGGAATATACAATCCCCAATTCAAATCGACGTGGATAGGTTGACACGAATTTTTG
ATAATATCATATCCAACAGTCTGCAATACACTCCATCAGGTGGTAATATTAGTATTACTGTAAAGGATGAAAAAAATTGT
ATTTCTTATGAGATTTGTGATTCTGGTAGAGGGTTTAGCTCTAAAGATTTAAAAAAGGCTCTTGATAAATTTTATCGAGG
AGATGAAGCTAGGCAGACAAAAGGTGGACATTCTGGTCTTGGGCTTTACATTGTCAAACAATTAGTGGAACAGCTTGGAG
GTTCTGTTAAAATAGAAAATTCAAAATCAGGTGGAGCATGTGTGAAATTTTGGCATAGCATTTAA

Upstream 100 bases:

>100_bases
AGCTAAGTTTTCTGCTGTGACACCAGAAAAAGAATATATTTCCACAGTTTGGGGTATTGGGTATAAGTGGAATAAAATGT
AAAGAGAGAGAGGTTATTAA

Downstream 100 bases:

>100_bases
CTATATATTTTAAATTAAAGGGGAGTGTCTCAAAGCGAAATTTTTAATTCGTGAGGCACTCTTTTTTTGTGAAGTTAAAT
ATATTTTCATTATTTCGACA

Product: lantibiotic resistance two-component sensor kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDIYPPNYYERQVPRIEKYINEKNITLLSQSNEEGLKRT
IRGDDMLYQVVDNNGNILYGTNPKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGR
FVFAVIIMALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYYSDNELGKLCSAFSEMKDELKGS
LSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDALIGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQ
LNLVPINLPEFLRQKVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISITVKDEKNC
ISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVEQLGGSVKIENSKSGGACVKFWHSI

Sequences:

>Translated_474_residues
MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDIYPPNYYERQVPRIEKYINEKNITLLSQSNEEGLKRT
IRGDDMLYQVVDNNGNILYGTNPKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGR
FVFAVIIMALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYYSDNELGKLCSAFSEMKDELKGS
LSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDALIGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQ
LNLVPINLPEFLRQKVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISITVKDEKNC
ISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVEQLGGSVKIENSKSGGACVKFWHSI
>Mature_474_residues
MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDIYPPNYYERQVPRIEKYINEKNITLLSQSNEEGLKRT
IRGDDMLYQVVDNNGNILYGTNPKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGR
FVFAVIIMALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYYSDNELGKLCSAFSEMKDELKGS
LSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDALIGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQ
LNLVPINLPEFLRQKVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISITVKDEKNC
ISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVEQLGGSVKIENSKSGGACVKFWHSI

Specific function: Member of the two-component regulatory system YycG/YycF involved in the regulation of the ftsAZ operon, the yocH and ykvT, cwlO, lytE, ydjM, yjeA, yoeB genes and the tagAB and tagDEF operons. Probably phosphorylates YycF [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=278, Percent_Identity=26.6187050359712, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1786600, Length=296, Percent_Identity=22.972972972973, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1789149, Length=338, Percent_Identity=21.8934911242604, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1787894, Length=278, Percent_Identity=23.7410071942446, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1786783, Length=262, Percent_Identity=24.8091603053435, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1786912, Length=222, Percent_Identity=24.7747747747748, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1790861, Length=255, Percent_Identity=24.7058823529412, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI48994928, Length=227, Percent_Identity=23.3480176211454, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790436, Length=219, Percent_Identity=25.1141552511416, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789808, Length=316, Percent_Identity=21.8354430379747, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788713, Length=220, Percent_Identity=21.8181818181818, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1790346, Length=244, Percent_Identity=24.1803278688525, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI145693157, Length=239, Percent_Identity=19.6652719665272, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788279, Length=234, Percent_Identity=26.9230769230769, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788549, Length=281, Percent_Identity=23.4875444839858, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 54322; Mature: 54322

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDIYPPNYYERQVPRIEKY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHH
INEKNITLLSQSNEEGLKRTIRGDDMLYQVVDNNGNILYGTNPKKLFKTKEELFNNFINK
HCCCCEEEEECCCHHHHHHHHCCCCEEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHH
TVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGRFVFAVIIMALFSPFLYIVGF
HHHCCCCEEEEEEECCCCCCCEEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
TRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYYSDNELGKLCSAFSEMKDELKGS
HHHHHHHHHHHCCCCCEEEEECHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCC
LSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDALIGNNTDDNEKLHRYLTVIRENTEK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHH
SAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQKVQDYELQAHQKEVELILKMQGNIQS
HHHHHHHHHHHHCHHHCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PIQIDVDRLTRIFDNIISNSLQYTPSGGNISITVKDEKNCISYEICDSGRGFSSKDLKKA
CCEECHHHHHHHHHHHHCCCCEECCCCCEEEEEEECCCCCEEEEEECCCCCCCHHHHHHH
LDKFYRGDEARQTKGGHSGLGLYIVKQLVEQLGGSVKIENSKSGGACVKFWHSI
HHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHCC
>Mature Secondary Structure
MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDIYPPNYYERQVPRIEKY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHH
INEKNITLLSQSNEEGLKRTIRGDDMLYQVVDNNGNILYGTNPKKLFKTKEELFNNFINK
HCCCCEEEEECCCHHHHHHHHCCCCEEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHH
TVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGRFVFAVIIMALFSPFLYIVGF
HHHCCCCEEEEEEECCCCCCCEEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
TRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYYSDNELGKLCSAFSEMKDELKGS
HHHHHHHHHHHCCCCCEEEEECHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCC
LSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDALIGNNTDDNEKLHRYLTVIRENTEK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHH
SAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQKVQDYELQAHQKEVELILKMQGNIQS
HHHHHHHHHHHHCHHHCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PIQIDVDRLTRIFDNIISNSLQYTPSGGNISITVKDEKNCISYEICDSGRGFSSKDLKKA
CCEECHHHHHHHHHHHHCCCCEECCCCCEEEEEEECCCCCEEEEEECCCCCCCHHHHHHH
LDKFYRGDEARQTKGGHSGLGLYIVKQLVEQLGGSVKIENSKSGGACVKFWHSI
HHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 9829949; 10878122; 11758928 [H]