BacMap

Definition	Clostridium difficile 630 chromosome, complete genome.
Accession	NC_009089
Length	4,290,252

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The map label for this gene is ppiB [C]

Identifier: 126697903

GI number: 126697903

Start: 398605

End: 399129

Strand: Direct

Name: ppiB [C]

Synonym: CD0331

Alternate gene names: 126697903

Gene position: 398605-399129 (Clockwise)

Preceding gene: 126697902

Following gene: 126697904

Centisome position: 9.29

GC content: 32.38

Gene sequence:

>525_bases
ATGGAAAATAAAAATCCTATAGTAACTATAGAAATGGAAAATGGAAAAGAAATCAAAATTGAGTTATATCCAAATATAGC
TCCAAATACAGTTAAAAACTTTGTATCATTAGTAAATGAAGGATATTACAATGGAATAATATTTCATAGAGTAATACCAG
GATTTATGATACAAGGAGGATGCCCAAATGGTACAGGAATGGGTGGTCCAGGTCACTCAATAAAAGGTGAATTTAGTGGT
AATGGATTTACTAACAATTTAAAACATGAAAGAGGAGTCATATCAATGGCTAGAACTATGGCACCTAATTCAGCTGGTTC
TCAATTTTTCATAATGCATAAAAACTCACCACACTTAGATGGTCAGTATGCAGGTTTTGGTAGAGTGATAGAAGGTATGG
ATACAGTTGATGAAATAGCATCAGTTAGAACAGATTCAGCTGATAAGCCACAAACTCCACAAGTTATGAAATCTGTTACT
GTAGAAACTTTTGGAATAGATTACTCAGATGTAGAAAAAAACTAA

Upstream 100 bases:

>100_bases
TATGAAATAGGAAAAAATATACTTGATAGACTTTAATTTAGAGTATAATATAATAAATATAATTATGTAATTTAACTAAT
GAAAAGGAAGGTAATAAATT

Downstream 100 bases:

>100_bases
AATTTATATAAAAACTAGATTTAGTTATAAATAAAAATTAAAAATATATATTACTTAAAAATGAGGATAAAGTGAAATGT
TATTATGTTTTTAGACAAAT

Product: peptidyl-prolyl cis-trans isomerase

Products: NA

Alternate protein names: Phosphatase; Peptidyl-prolyl cis-trans isomerase; PPIase; Rotamase [H]

Number of amino acids: Translated: 174; Mature: 174

Protein sequence:

>174_residues
MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYNGIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSG
NGFTNNLKHERGVISMARTMAPNSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQVMKSVT
VETFGIDYSDVEKN

Sequences:

>Translated_174_residues
MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYNGIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSG
NGFTNNLKHERGVISMARTMAPNSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQVMKSVT
VETFGIDYSDVEKN
>Mature_174_residues
MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYNGIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSG
NGFTNNLKHERGVISMARTMAPNSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQVMKSVT
VETFGIDYSDVEKN

Specific function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides [H]

COG id: COG0652

COG function: function code O; Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase cyclophilin-type domain [H]

Homologues:

Organism=Homo sapiens, GI19557636, Length=156, Percent_Identity=43.5897435897436, Blast_Score=120, Evalue=7e-28,
Organism=Homo sapiens, GI24308049, Length=148, Percent_Identity=43.2432432432432, Blast_Score=106, Evalue=1e-23,
Organism=Homo sapiens, GI7706339, Length=146, Percent_Identity=43.8356164383562, Blast_Score=106, Evalue=1e-23,
Organism=Homo sapiens, GI64276486, Length=149, Percent_Identity=38.255033557047, Blast_Score=97, Evalue=9e-21,
Organism=Homo sapiens, GI22547212, Length=150, Percent_Identity=38.6666666666667, Blast_Score=89, Evalue=2e-18,
Organism=Homo sapiens, GI7657473, Length=150, Percent_Identity=38.6666666666667, Blast_Score=89, Evalue=2e-18,
Organism=Homo sapiens, GI6631100, Length=155, Percent_Identity=36.7741935483871, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI42560244, Length=145, Percent_Identity=39.3103448275862, Blast_Score=87, Evalue=5e-18,
Organism=Homo sapiens, GI4505991, Length=141, Percent_Identity=46.0992907801418, Blast_Score=87, Evalue=6e-18,
Organism=Homo sapiens, GI4758950, Length=163, Percent_Identity=40.4907975460123, Blast_Score=87, Evalue=6e-18,
Organism=Homo sapiens, GI5031987, Length=154, Percent_Identity=43.5064935064935, Blast_Score=87, Evalue=8e-18,
Organism=Homo sapiens, GI5454154, Length=169, Percent_Identity=34.3195266272189, Blast_Score=84, Evalue=7e-17,
Organism=Homo sapiens, GI22547215, Length=150, Percent_Identity=38, Blast_Score=84, Evalue=8e-17,
Organism=Homo sapiens, GI10863927, Length=140, Percent_Identity=41.4285714285714, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI310127646, Length=147, Percent_Identity=40.8163265306122, Blast_Score=81, Evalue=4e-16,
Organism=Homo sapiens, GI310114371, Length=147, Percent_Identity=40.8163265306122, Blast_Score=81, Evalue=4e-16,
Organism=Homo sapiens, GI310119221, Length=147, Percent_Identity=40.8163265306122, Blast_Score=81, Evalue=4e-16,
Organism=Homo sapiens, GI4826932, Length=149, Percent_Identity=40.9395973154362, Blast_Score=80, Evalue=8e-16,
Organism=Homo sapiens, GI256017253, Length=146, Percent_Identity=41.0958904109589, Blast_Score=77, Evalue=6e-15,
Organism=Homo sapiens, GI221139811, Length=146, Percent_Identity=41.0958904109589, Blast_Score=77, Evalue=6e-15,
Organism=Homo sapiens, GI256017251, Length=146, Percent_Identity=41.0958904109589, Blast_Score=77, Evalue=6e-15,
Organism=Homo sapiens, GI150418007, Length=149, Percent_Identity=40.2684563758389, Blast_Score=77, Evalue=7e-15,
Organism=Homo sapiens, GI303305009, Length=159, Percent_Identity=37.1069182389937, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI45439316, Length=147, Percent_Identity=38.7755102040816, Blast_Score=76, Evalue=1e-14,
Organism=Homo sapiens, GI5174637, Length=149, Percent_Identity=38.255033557047, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI310110104, Length=147, Percent_Identity=39.4557823129252, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI178057341, Length=141, Percent_Identity=39.0070921985816, Blast_Score=74, Evalue=7e-14,
Organism=Homo sapiens, GI310129267, Length=147, Percent_Identity=38.7755102040816, Blast_Score=74, Evalue=7e-14,
Organism=Homo sapiens, GI310120620, Length=147, Percent_Identity=38.7755102040816, Blast_Score=74, Evalue=7e-14,
Organism=Homo sapiens, GI20911035, Length=132, Percent_Identity=36.3636363636364, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI14277126, Length=142, Percent_Identity=35.9154929577465, Blast_Score=71, Evalue=6e-13,
Organism=Homo sapiens, GI169164185, Length=147, Percent_Identity=38.7755102040816, Blast_Score=71, Evalue=6e-13,
Organism=Homo sapiens, GI169163623, Length=147, Percent_Identity=38.7755102040816, Blast_Score=71, Evalue=6e-13,
Organism=Homo sapiens, GI169163387, Length=147, Percent_Identity=38.7755102040816, Blast_Score=71, Evalue=6e-13,
Organism=Homo sapiens, GI209417936, Length=140, Percent_Identity=37.8571428571429, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI30102944, Length=140, Percent_Identity=37.8571428571429, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI219842199, Length=140, Percent_Identity=37.8571428571429, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI27735045, Length=137, Percent_Identity=35.036496350365, Blast_Score=67, Evalue=8e-12,
Organism=Homo sapiens, GI310132444, Length=140, Percent_Identity=37.1428571428571, Blast_Score=64, Evalue=5e-11,
Organism=Homo sapiens, GI310113152, Length=140, Percent_Identity=37.1428571428571, Blast_Score=64, Evalue=5e-11,
Organism=Escherichia coli, GI1789763, Length=167, Percent_Identity=42.5149700598802, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI1786736, Length=168, Percent_Identity=41.0714285714286, Blast_Score=102, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71980594, Length=155, Percent_Identity=43.8709677419355, Blast_Score=124, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17539496, Length=165, Percent_Identity=43.030303030303, Blast_Score=122, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI71980590, Length=148, Percent_Identity=44.5945945945946, Blast_Score=119, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17506311, Length=148, Percent_Identity=39.8648648648649, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17532641, Length=172, Percent_Identity=36.046511627907, Blast_Score=96, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17551282, Length=155, Percent_Identity=38.7096774193548, Blast_Score=89, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17551284, Length=155, Percent_Identity=38.7096774193548, Blast_Score=89, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17552782, Length=164, Percent_Identity=40.8536585365854, Blast_Score=87, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71997782, Length=125, Percent_Identity=40, Blast_Score=86, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17552780, Length=164, Percent_Identity=42.0731707317073, Blast_Score=85, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17539498, Length=140, Percent_Identity=44.2857142857143, Blast_Score=85, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17506307, Length=172, Percent_Identity=39.5348837209302, Blast_Score=83, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17532643, Length=168, Percent_Identity=39.2857142857143, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17559074, Length=149, Percent_Identity=40.2684563758389, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17566266, Length=147, Percent_Identity=41.4965986394558, Blast_Score=81, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17565860, Length=160, Percent_Identity=38.125, Blast_Score=81, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17552784, Length=147, Percent_Identity=38.0952380952381, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI32563659, Length=150, Percent_Identity=32, Blast_Score=69, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6323562, Length=147, Percent_Identity=44.2176870748299, Blast_Score=93, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321848, Length=155, Percent_Identity=43.2258064516129, Blast_Score=92, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6320510, Length=163, Percent_Identity=40.4907975460123, Blast_Score=89, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320359, Length=150, Percent_Identity=40.6666666666667, Blast_Score=83, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323246, Length=187, Percent_Identity=34.2245989304813, Blast_Score=80, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6322492, Length=185, Percent_Identity=30.2702702702703, Blast_Score=64, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17986117, Length=159, Percent_Identity=44.6540880503145, Blast_Score=123, Evalue=5e-29,
Organism=Drosophila melanogaster, GI19922376, Length=145, Percent_Identity=39.3103448275862, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24652460, Length=156, Percent_Identity=37.8205128205128, Blast_Score=106, Evalue=8e-24,
Organism=Drosophila melanogaster, GI19922912, Length=136, Percent_Identity=44.1176470588235, Blast_Score=87, Evalue=7e-18,
Organism=Drosophila melanogaster, GI21357249, Length=114, Percent_Identity=39.4736842105263, Blast_Score=87, Evalue=7e-18,
Organism=Drosophila melanogaster, GI20130249, Length=150, Percent_Identity=42, Blast_Score=87, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24664125, Length=172, Percent_Identity=40.1162790697674, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21357783, Length=172, Percent_Identity=40.1162790697674, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI45549139, Length=138, Percent_Identity=43.4782608695652, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24650807, Length=148, Percent_Identity=35.8108108108108, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17136360, Length=150, Percent_Identity=36.6666666666667, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17647301, Length=162, Percent_Identity=38.2716049382716, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24586038, Length=166, Percent_Identity=35.5421686746988, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24661775, Length=190, Percent_Identity=32.6315789473684, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21355237, Length=190, Percent_Identity=32.6315789473684, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24661771, Length=190, Percent_Identity=32.6315789473684, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015891
- InterPro:   IPR023214
- InterPro:   IPR013200
- InterPro:   IPR006379
- InterPro:   IPR000150
- InterPro:   IPR002130 [H]

Pfam domain/function: PF08282 Hydrolase_3; PF00160 Pro_isomerase [H]

EC number: =5.2.1.8 [H]

Molecular weight: Translated: 18973; Mature: 18973

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS50072 CSA_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYNGIIFHRVIPGFMIQGG
CCCCCCEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEHHHHCCCEEEECC
CPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAPNSAGSQFFIMHKNSPHLD
CCCCCCCCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCC
GQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQVMKSVTVETFGIDYSDVEKN
CCCCCHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHEECCCHHHCCCC
>Mature Secondary Structure
MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYNGIIFHRVIPGFMIQGG
CCCCCCEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEHHHHCCCEEEECC
CPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAPNSAGSQFFIMHKNSPHLD
CCCCCCCCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCC
GQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQVMKSVTVETFGIDYSDVEKN
CCCCCHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHEECCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA