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The map label for this gene is rbsB [H]

Identifier: 126697872

GI number: 126697872

Start: 361084

End: 362046

Strand: Direct

Name: rbsB [H]

Synonym: CD0300

Alternate gene names: 126697872

Gene position: 361084-362046 (Clockwise)

Preceding gene: 126697871

Following gene: 126697873

Centisome position: 8.42

GC content: 31.57

Gene sequence:

>963_bases
ATGAGAGTGAGAAAAATTTTATCTTTAGGAATTGCTGGAATACTAGCAATAGGAATGTTAACAGGGTGTTCAATGGAAGG
ACCAAGTAAATCAGATAATAAAGGTGGTTCAGACAAGAAAGATTTAACTATTGGAGTATCTACAATAACTCTTCAACATC
AATTTTTTATAGATATTGATGAGGGAATAAAAGAAAAAGCTAAAGAATTAGGTGTGAAAGTTATAGTAAATGACCCAGAC
CAAGATGTAGCTAAGCAAACATCAGCAATAGAAGATTTTATACAACAAAATGTAGATGGAATGATAGTACTTGGAACTGA
TAATTCGGCTATAGTACCAGCAGTAGAAGGAGCATTTGAAAAAATGCCAGTAGTTACAGTAGATGCTGTATTAAATACTG
AAAATATTACAAGTTATGTAGGAACAGTTAGTTATGATGCTGGTAAAAAATTAGGGGAATACACTAAAAAATACATAGAT
GAAAAGTTAGGTGGAAAGTCAGAAATAGCAATAGTGACAGATTTAAAATCTCAAATACAAATGCAGAGAATAGATGGATT
TAAAGATGCATTAAAAGGTTCAGCAAATGTTAAGATATTAAATTCACAACCAGGATATGACAGAGAAGAATCTCTTAATA
CAGTAGAAAACTTAATTCAATCTAATCCAGATGTAGATATAATATATGCAACAGCTGAAAATAGTGTACTAGGAGCTAAA
GCTGCACTTGAATCTGCAAAAAATAAGGATGTTAAGATTGTAGGATTTGACTTAACTGAAGAAGCATCAAGTGGAATAAC
AGATGGAACTATTCTTGCTATGATTCAACAACAACCTAAAGAGATGGGACGTTTAGCTGTAGAAGCAGTAGTAAAAGCTA
TAAAAGGGGAAAAGGTAGAAAAGAATATACCAGTACCAGCTTTACTTTATGATAAGGAAAATATAAAAGACTTTAAGAAT
TAG

Upstream 100 bases:

>100_bases
AATCTGGTATGCCATATAAAGAAGATGTTGAAAAATATTTAAATAATAAATAAAAATCTAATTTGTTCAAATTTAAAACT
ATTAAAAATGGAGGTTAATT

Downstream 100 bases:

>100_bases
TAAAATAAAATGAGATTATTTGAAAGAAATCCTATTTAAAAAGGAGTGTCATTATGAGTAATATAATTCTGAAGTTAAGT
AATATTGCTAAAGAATTTCC

Product: D-ribose ABC transporter substrate-binding protein

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTIGVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPD
QDVAKQTSAIEDFIQQNVDGMIVLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENLIQSNPDVDIIYATAENSVLGAK
AALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQQQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN

Sequences:

>Translated_320_residues
MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTIGVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPD
QDVAKQTSAIEDFIQQNVDGMIVLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENLIQSNPDVDIIYATAENSVLGAK
AALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQQQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN
>Mature_320_residues
MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTIGVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPD
QDVAKQTSAIEDFIQQNVDGMIVLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENLIQSNPDVDIIYATAENSVLGAK
AALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQQQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=265, Percent_Identity=30.9433962264151, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1790526, Length=266, Percent_Identity=28.5714285714286, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788898, Length=288, Percent_Identity=26.0416666666667, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787228, Length=300, Percent_Identity=21.6666666666667, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790674, Length=253, Percent_Identity=23.3201581027668, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 34496; Mature: 34496

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTIGVSTITLQHQFFIDID
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEEEEEEEHH
EGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMIVLGTDNSAIVPAVEGAFE
HHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCEECHHHHHHHH
KMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYIDEKLGGKSEIAIVTDLKSQIQ
HCCEEEEEEEECCHHHHHHHHEEECHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHH
MQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENLIQSNPDVDIIYATAENSVLGAK
HHHHCCHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHH
AALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQQQPKEMGRLAVEAVVKAIKGEKVE
HHHHHCCCCCEEEEEECCHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCC
KNIPVPALLYDKENIKDFKN
CCCCCCEEEECCHHHHHCCC
>Mature Secondary Structure
MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTIGVSTITLQHQFFIDID
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEEEEEEEHH
EGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMIVLGTDNSAIVPAVEGAFE
HHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCEECHHHHHHHH
KMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYIDEKLGGKSEIAIVTDLKSQIQ
HCCEEEEEEEECCHHHHHHHHEEECHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHH
MQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENLIQSNPDVDIIYATAENSVLGAK
HHHHCCHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHH
AALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQQQPKEMGRLAVEAVVKAIKGEKVE
HHHHHCCCCCEEEEEECCHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCC
KNIPVPALLYDKENIKDFKN
CCCCCCEEEECCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]