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The map label for this gene is ybiK [C]

Identifier: 126697711

GI number: 126697711

Start: 185303

End: 186262

Strand: Direct

Name: ybiK [C]

Synonym: CD0139

Alternate gene names: 126697711

Gene position: 185303-186262 (Clockwise)

Preceding gene: 126697710

Following gene: 126697712

Centisome position: 4.32

GC content: 35.62

Gene sequence:

>960_bases
ATGTGGGGAATGATAGCGACATGGAGAATGGCCCTAGAAGGAATAACTGAGGCATCAGAACATTTAGAAAAAGGCATGGA
AGCAGGTGATGCTGTAGAGCTTGCTGTAAGAAGGGTTGAAGACTATCCATTCTATAAATCGGTTGGATATGGTGGACTTC
CAAATGAAAATTGTGAGGTAGAACTTGACGCTGCATTTATGGATGGTAACACATTAGATATTGGCGCAGTTGCTGGTATA
AGAGATTATTCTAATCCTGTCAGCATAGCTAGGAAGTTAAGCTATGAGAAGGTAAATAATTTTTTAGTTGGAATAGGTGC
TGAAGATTATGCTCATAAAAATGGATTTGAAAGAAAAAATATGTTGACTGATAGAGCGAAGTTACATTATAAAAAGAGAA
AAAAAGAGACTTTAGACAAAGGATTAAGCCCATATGCTGGTCATGATACTGTTGGTATGATATCTTTAGACAAAACTGGA
AAAATGTGTGCAGCTACATCAACAAGTGGATTGTTTATGAAGAAAAGAGGTAGAATAGGAGATTCTGCACTATCTGGTTC
AGGATTCTATGTTGATAGTAGTATAGGAGGAGCTACAGCTACTGGTTTGGGAGAAGATTTAATGAAGGGATGTATATCTT
ATGAGATAGTTAGACTTATGAAAGAAGGGAAGCATCCACAAGAAGCATGTGATATTGCAGTAAATGAACTTGATAAACAG
CTTATCAAAAGAAGAGGAAAAGCTGGTGATTTATCATTAGTTGCCATGAATAATAAAGGTGAATGGGGAGTAGCTACAAA
TATAGCTGATTTTACTTTTTCAGTAGTAACTAAAGAAGAATCTCCTACTGTATATATTTCAAATAGAGTAGATGGAAAGA
CTATACATAAGGTTATATCAAATGAGTGGATGGAAGCATATGAGGCTAGAATAAAAGCACCAATAGAAGTAAGTGAGTAA

Upstream 100 bases:

>100_bases
ATTAATAAGAGTATTAATTATTAGCAAGTTTAAATATTATACTATTTATAATAAGCATACATTAGTAAAATCTATATAAT
TTAAGGAGGGGAATTTTAAC

Downstream 100 bases:

>100_bases
AGTAGTGGTTACATAATTAAAATAGAGTTTTATAATAAGGAGATTTTTATGAATATCATAAGTAATAAAATAGATGAGTT
AAAAGAAGATTTATTAAGTG

Product: glycosylasparaginase

Products: NA

Alternate protein names: Aspartylglucosaminidase; AGA; Glycosylasparaginase; N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPFYKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGI
RDYSNPVSIARKLSYEKVNNFLVGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVRLMKEGKHPQEACDIAVNELDKQ
LIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVVTKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE

Sequences:

>Translated_319_residues
MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPFYKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGI
RDYSNPVSIARKLSYEKVNNFLVGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVRLMKEGKHPQEACDIAVNELDKQ
LIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVVTKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE
>Mature_319_residues
MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPFYKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGI
RDYSNPVSIARKLSYEKVNNFLVGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVRLMKEGKHPQEACDIAVNELDKQ
LIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVVTKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE

Specific function: Cleaves the GlcNAc-Asn bond which joins oligosaccharides to the peptide of asparagine-linked glycoproteins. Requires that the glycosylated asparagine moiety is not substituted on its N- (R1) and C- (R2) terminus [H]

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI285002251, Length=317, Percent_Identity=32.807570977918, Blast_Score=131, Evalue=8e-31,
Organism=Homo sapiens, GI145275202, Length=244, Percent_Identity=34.0163934426229, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI145275200, Length=244, Percent_Identity=34.0163934426229, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI285002253, Length=317, Percent_Identity=31.5457413249211, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI98991769, Length=242, Percent_Identity=30.5785123966942, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1787050, Length=268, Percent_Identity=34.7014925373134, Blast_Score=124, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17569239, Length=279, Percent_Identity=29.0322580645161, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI32564649, Length=199, Percent_Identity=31.1557788944724, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI221330102, Length=282, Percent_Identity=32.2695035460993, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24652135, Length=282, Percent_Identity=32.2695035460993, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI18921183, Length=265, Percent_Identity=32.8301886792453, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24655553, Length=266, Percent_Identity=27.8195488721804, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24657358, Length=255, Percent_Identity=27.0588235294118, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21357893, Length=272, Percent_Identity=30.1470588235294, Blast_Score=95, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.5.1.26 [H]

Molecular weight: Translated: 34961; Mature: 34961

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPFYKSVGYGGLPNENCEV
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHCCCCCCCCCCCEE
ELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFLVGIGAEDYAHKNGFERKN
EEEEEEECCCEEEEHHHHCCHHCCCHHHHHHHHHHHHHCCEEEEECCHHHHHCCCCCHHH
MLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTGKMCAATSTSGLFMKKRGRIG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEECCCCCEEEECCCCCC
DSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVRLMKEGKHPQEACDIAVNELDKQ
CCCCCCCCEEEECCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVVTKEESPTVYISNRVDGKTIHKVIS
HHHHCCCCCCEEEEEECCCCCCCEECCHHHEEEEEEEECCCCEEEEECCCCHHHHHHHHH
NEWMEAYEARIKAPIEVSE
HHHHHHHHHHHCCCCEECC
>Mature Secondary Structure
MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPFYKSVGYGGLPNENCEV
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHCCCCCCCCCCCEE
ELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFLVGIGAEDYAHKNGFERKN
EEEEEEECCCEEEEHHHHCCHHCCCHHHHHHHHHHHHHCCEEEEECCHHHHHCCCCCHHH
MLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTGKMCAATSTSGLFMKKRGRIG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEECCCCCEEEECCCCCC
DSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVRLMKEGKHPQEACDIAVNELDKQ
CCCCCCCCEEEECCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVVTKEESPTVYISNRVDGKTIHKVIS
HHHHCCCCCCEEEEEECCCCCCCEECCHHHEEEEEEEECCCCEEEEECCCCHHHHHHHHH
NEWMEAYEARIKAPIEVSE
HHHHHHHHHHHCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7840643; 8250923; 9541410 [H]