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The map label for this gene is tetA [H]

Identifier: 126697224

GI number: 126697224

Start: 1613061

End: 1614329

Strand: Direct

Name: tetA [H]

Synonym: P9301_18861

Alternate gene names: 126697224

Gene position: 1613061-1614329 (Clockwise)

Preceding gene: 126697222

Following gene: 126697225

Centisome position: 98.24

GC content: 32.55

Gene sequence:

>1269_bases
GTGAAAAAAAGTTTATTGAAGCCAAATAAAAAATTTACTCTTCTGAGTGCTTTTATCACCCTTCTCAATGATCGATTAAG
TGAAAGTATATTATTACCCATTTTACCCTCCTTTGTTTTACTCTTTGACTCTAAAGCTAGTACATATGGCTTATTATCAT
GTACTTACCAGTTAGCCCAATTTACAGCTTCTCCTTTTATAGGACTTATGAGTGATAGATACGGTAGAAGACCTGTCACT
CTTTTTTGTATTACAGGTTCAGTAATAGGAATATCAATATTATCTTTTACAGTTCTTTTTAATTGGTCTAATTCAATAGC
CTCTATCCCGCTATTTTTATTATTCTTAGCGAGACTCATTGACGGCTTAAGTGGTGGAACTGCAGCCACAGCAACAACTA
TTCTTGCAGACATTTCAAGCCCTGAAAAAAGAGCAAAAACATTTGGTCTTATTGGTGTAGCTTTTGGTTTAAGCTTTTTC
TTAGGTAATATTTTCGTTGTAATATTTGCAAAAAATACAAACAATAACTTTATCATTCCAGTGTTAATAGCCTCAATGAT
TCCAATAATAAATTTCTTACTTGTATTTTTTTACTTACCAGAAACCAAGCCTAAAAGCGACTCAAATAAAACAAAAACTA
TTTTAAAAAACCCTTTAAAAGAGCTATTTACAGTTTTCAAAGAAGAAAAGATTAAAAAATTATCATTAGCTTTTTTTATT
TACTTTATTGCTTTTACTGGATTGACCAATATCCTTATATTTTTCCTTCAAGAATCCTTAAACTGGACGACAAAAGCATC
AAGTGGAACTCTGGTTGTAGTTGGAATCATTGCGATTATCGTACAGGGAGGACTAATTGGGCCTCTAGTAAAGAAATTTG
GCGAAATGCGATTAACACTTATTGGGTCAGGCTTTATTCTTGTGGCATGTGCTCTTTTAATAACTGCCCCAAAAGATAAT
GCGACAATTAATATTTATTCAGCTGTTTCATTTTTAGCCGTTGGGGCAGGGTTAATTACGCCCACCTTAAGAGCATTAAT
ATCGAAGAAATTAGAAGTTGATAAACAAGGATCAATTTTAAGTAATCTTCAGGGTTTACAGAGCCTCGGAGGAGTTTTAG
GAATTGCAATGGCAGGAAGGGTTTATGATGGTTTTGGTCCTAAATCACCTTTTATCGCTGGTTCCATAATCTTACTTTTC
ATGATATATCTTATTGCAGAGGGTAAAAGTAATAATTCTTTTAAAAATCAAAAATCAAAAGCATTTTAA

Upstream 100 bases:

>100_bases
GGTCACCAGGACCAGCTCCAACTAAATAAACAATGCCAGGCACATTAATCTCCATTAACAAGTATGGTAATAGAAGTTAA
TCGCAATGAAGGTAAATATT

Downstream 100 bases:

>100_bases
TGAAAATCCAGGCTGATATTTTTATTAATAGAGAATTAAGTTGGATTGAATTCAATAAAAGAGTACTCCTAACTGGTATG
GAAAAGGAGTACAAAATCCT

Product: major facilitator superfamily multidrug-efflux transporter

Products: NA

Alternate protein names: TetA(B); Metal-tetracycline/H(+) antiporter [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MKKSLLKPNKKFTLLSAFITLLNDRLSESILLPILPSFVLLFDSKASTYGLLSCTYQLAQFTASPFIGLMSDRYGRRPVT
LFCITGSVIGISILSFTVLFNWSNSIASIPLFLLFLARLIDGLSGGTAATATTILADISSPEKRAKTFGLIGVAFGLSFF
LGNIFVVIFAKNTNNNFIIPVLIASMIPIINFLLVFFYLPETKPKSDSNKTKTILKNPLKELFTVFKEEKIKKLSLAFFI
YFIAFTGLTNILIFFLQESLNWTTKASSGTLVVVGIIAIIVQGGLIGPLVKKFGEMRLTLIGSGFILVACALLITAPKDN
ATINIYSAVSFLAVGAGLITPTLRALISKKLEVDKQGSILSNLQGLQSLGGVLGIAMAGRVYDGFGPKSPFIAGSIILLF
MIYLIAEGKSNNSFKNQKSKAF

Sequences:

>Translated_422_residues
MKKSLLKPNKKFTLLSAFITLLNDRLSESILLPILPSFVLLFDSKASTYGLLSCTYQLAQFTASPFIGLMSDRYGRRPVT
LFCITGSVIGISILSFTVLFNWSNSIASIPLFLLFLARLIDGLSGGTAATATTILADISSPEKRAKTFGLIGVAFGLSFF
LGNIFVVIFAKNTNNNFIIPVLIASMIPIINFLLVFFYLPETKPKSDSNKTKTILKNPLKELFTVFKEEKIKKLSLAFFI
YFIAFTGLTNILIFFLQESLNWTTKASSGTLVVVGIIAIIVQGGLIGPLVKKFGEMRLTLIGSGFILVACALLITAPKDN
ATINIYSAVSFLAVGAGLITPTLRALISKKLEVDKQGSILSNLQGLQSLGGVLGIAMAGRVYDGFGPKSPFIAGSIILLF
MIYLIAEGKSNNSFKNQKSKAF
>Mature_422_residues
MKKSLLKPNKKFTLLSAFITLLNDRLSESILLPILPSFVLLFDSKASTYGLLSCTYQLAQFTASPFIGLMSDRYGRRPVT
LFCITGSVIGISILSFTVLFNWSNSIASIPLFLLFLARLIDGLSGGTAATATTILADISSPEKRAKTFGLIGVAFGLSFF
LGNIFVVIFAKNTNNNFIIPVLIASMIPIINFLLVFFYLPETKPKSDSNKTKTILKNPLKELFTVFKEEKIKKLSLAFFI
YFIAFTGLTNILIFFLQESLNWTTKASSGTLVVVGIIAIIVQGGLIGPLVKKFGEMRLTLIGSGFILVACALLITAPKDN
ATINIYSAVSFLAVGAGLITPTLRALISKKLEVDKQGSILSNLQGLQSLGGVLGIAMAGRVYDGFGPKSPFIAGSIILLF
MIYLIAEGKSNNSFKNQKSKAF

Specific function: Resistance to tetracycline by an active tetracycline efflux. This is an energy-dependent process that decreases the accumulation of the antibiotic in whole cells. This protein functions as a metal-tetracycline/H(+) antiporter [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Homo sapiens, GI225703104, Length=253, Percent_Identity=27.2727272727273, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI225703102, Length=253, Percent_Identity=27.2727272727273, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI39753965, Length=361, Percent_Identity=21.0526315789474, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI94721311, Length=382, Percent_Identity=21.2041884816754, Blast_Score=65, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17551570, Length=366, Percent_Identity=24.5901639344262, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI71996331, Length=338, Percent_Identity=20.7100591715976, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI17738085, Length=373, Percent_Identity=26.0053619302949, Blast_Score=122, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 45745; Mature: 45745

Theoretical pI: Translated: 10.54; Mature: 10.54

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSLLKPNKKFTLLSAFITLLNDRLSESILLPILPSFVLLFDSKASTYGLLSCTYQLAQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
FTASPFIGLMSDRYGRRPVTLFCITGSVIGISILSFTVLFNWSNSIASIPLFLLFLARLI
HHHCCHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHH
DGLSGGTAATATTILADISSPEKRAKTFGLIGVAFGLSFFLGNIFVVIFAKNTNNNFIIP
CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCEEHH
VLIASMIPIINFLLVFFYLPETKPKSDSNKTKTILKNPLKELFTVFKEEKIKKLSLAFFI
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YFIAFTGLTNILIFFLQESLNWTTKASSGTLVVVGIIAIIVQGGLIGPLVKKFGEMRLTL
HHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCEEEEE
IGSGFILVACALLITAPKDNATINIYSAVSFLAVGAGLITPTLRALISKKLEVDKQGSIL
ECCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
SNLQGLQSLGGVLGIAMAGRVYDGFGPKSPFIAGSIILLFMIYLIAEGKSNNSFKNQKSK
HHHHHHHHHHHHHHHHHHCHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
AF
CC
>Mature Secondary Structure
MKKSLLKPNKKFTLLSAFITLLNDRLSESILLPILPSFVLLFDSKASTYGLLSCTYQLAQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
FTASPFIGLMSDRYGRRPVTLFCITGSVIGISILSFTVLFNWSNSIASIPLFLLFLARLI
HHHCCHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHH
DGLSGGTAATATTILADISSPEKRAKTFGLIGVAFGLSFFLGNIFVVIFAKNTNNNFIIP
CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCEEHH
VLIASMIPIINFLLVFFYLPETKPKSDSNKTKTILKNPLKELFTVFKEEKIKKLSLAFFI
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YFIAFTGLTNILIFFLQESLNWTTKASSGTLVVVGIIAIIVQGGLIGPLVKKFGEMRLTL
HHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCEEEEE
IGSGFILVACALLITAPKDNATINIYSAVSFLAVGAGLITPTLRALISKKLEVDKQGSIL
ECCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
SNLQGLQSLGGVLGIAMAGRVYDGFGPKSPFIAGSIILLFMIYLIAEGKSNNSFKNQKSK
HHHHHHHHHHHHHHHHHHCHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
AF
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 6319234; 6298728; 1848846; 2168416; 8995300 [H]