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The map label for this gene is miaA

Identifier: 126697164

GI number: 126697164

Start: 1547375

End: 1548274

Strand: Direct

Name: miaA

Synonym: P9301_18261

Alternate gene names: 126697164

Gene position: 1547375-1548274 (Clockwise)

Preceding gene: 126697158

Following gene: 126697165

Centisome position: 94.24

GC content: 30.67

Gene sequence:

>900_bases
ATGTCTTCACATCCACCTCATGTAATAGTTTTAATTGGGCCTACTGCAAGTGGCAAAACAGAATTAGCTATTGAAATTGC
AGAATATTTTAAAACTAATATACACAATATCGATTCAAGGCAAATATATAAGTCCATGGATATTGGAACAGCCAAGCCAT
CTAAAATCCAACAAAAAAAAATACGGCATTTTTTAATAGATATTGAAGAACCTATCAATCCAATTAATGTAAAACAATTT
CAAGAAATTGCTCAGAAATCGATCAAAGAAGAAATTAAAAAAGATAAATTACCTTTTCTTGTTGGAGGAAGTGGTTTGTA
TATGAACTCAATAACAAAAGGTTTTTTCGTACCAGATGTCCCTCCTCAAAATAATTTAAGAAAACAATTAGAAGAACTTG
GTCAGGAAAAATGTTGGGACCTGTTAGAAAATTGTGATCCATTATCAACAAAAAAAATCAATTTTGCTGACCACGTTCGA
ACAATAAGAGCTTTAGAAGTCTTCTACGTAACAGGTAAGCCTTTATCAACTCTGAAAGTTCAGAAACCACCTAACTGGAA
AATCTTAGAGCTTGGATTAGACAGAGGTAATTTAAAAGAAAGAATTTTTCAAAGAACAAAAAATATGTTTTTATCTGGAA
TAATTGATGAGACGAATCACCTTATCTCTAAATACGGATTTGATTTGCCAATATTAGAAACAATTGGCTATCGAGAAGCT
AGAAATGTTTTAAATAACCATTCAACAATTGACAAAGCGATTGAGTTAACTGCAACAAAAACAATCCAATTTGCCAAAAG
ACAAAAAACTTGGTTTCGTAATAAAAATAATCCTCTTTGGCTTAATAACAAAAACCCGCTAAAAGATGCAATAATTAAGA
TAGAGTCTTTTTTAAGCTAA

Upstream 100 bases:

>100_bases
TCATTAAACTTACCACCGCAATAAGGTAAAGGCTCGGAAATCAATGAAAAAATTAAACACTTTAATTATATAGCTAATAT
TTTTTTCTTCAGAAATTCAT

Downstream 100 bases:

>100_bases
CTTTATAAAAATAGATTTTGTTCATCATCCAATCAATTTATTTAATTAACTGAATGCCCCCACGCCCACGCTTTGACCGA
AGAGCTCCAGTTAGAGAGCT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MSSHPPHVIVLIGPTASGKTELAIEIAEYFKTNIHNIDSRQIYKSMDIGTAKPSKIQQKKIRHFLIDIEEPINPINVKQF
QEIAQKSIKEEIKKDKLPFLVGGSGLYMNSITKGFFVPDVPPQNNLRKQLEELGQEKCWDLLENCDPLSTKKINFADHVR
TIRALEVFYVTGKPLSTLKVQKPPNWKILELGLDRGNLKERIFQRTKNMFLSGIIDETNHLISKYGFDLPILETIGYREA
RNVLNNHSTIDKAIELTATKTIQFAKRQKTWFRNKNNPLWLNNKNPLKDAIIKIESFLS

Sequences:

>Translated_299_residues
MSSHPPHVIVLIGPTASGKTELAIEIAEYFKTNIHNIDSRQIYKSMDIGTAKPSKIQQKKIRHFLIDIEEPINPINVKQF
QEIAQKSIKEEIKKDKLPFLVGGSGLYMNSITKGFFVPDVPPQNNLRKQLEELGQEKCWDLLENCDPLSTKKINFADHVR
TIRALEVFYVTGKPLSTLKVQKPPNWKILELGLDRGNLKERIFQRTKNMFLSGIIDETNHLISKYGFDLPILETIGYREA
RNVLNNHSTIDKAIELTATKTIQFAKRQKTWFRNKNNPLWLNNKNPLKDAIIKIESFLS
>Mature_298_residues
SSHPPHVIVLIGPTASGKTELAIEIAEYFKTNIHNIDSRQIYKSMDIGTAKPSKIQQKKIRHFLIDIEEPINPINVKQFQ
EIAQKSIKEEIKKDKLPFLVGGSGLYMNSITKGFFVPDVPPQNNLRKQLEELGQEKCWDLLENCDPLSTKKINFADHVRT
IRALEVFYVTGKPLSTLKVQKPPNWKILELGLDRGNLKERIFQRTKNMFLSGIIDETNHLISKYGFDLPILETIGYREAR
NVLNNHSTIDKAIELTATKTIQFAKRQKTWFRNKNNPLWLNNKNPLKDAIIKIESFLS

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=308, Percent_Identity=25.974025974026, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1790613, Length=303, Percent_Identity=32.013201320132, Blast_Score=160, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI25144712, Length=314, Percent_Identity=26.1146496815287, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324848, Length=286, Percent_Identity=27.2727272727273, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24649830, Length=260, Percent_Identity=29.2307692307692, Blast_Score=115, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_PROM0 (A3PFC4)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001092050.1
- ProteinModelPortal:   A3PFC4
- SMR:   A3PFC4
- STRING:   A3PFC4
- GeneID:   4911208
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_18261
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   PMAR167546:P9301ORF_1865-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34373; Mature: 34242

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSHPPHVIVLIGPTASGKTELAIEIAEYFKTNIHNIDSRQIYKSMDIGTAKPSKIQQKK
CCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHH
IRHFLIDIEEPINPINVKQFQEIAQKSIKEEIKKDKLPFLVGGSGLYMNSITKGFFVPDV
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEHHHCCCCCCCCCC
PPQNNLRKQLEELGQEKCWDLLENCDPLSTKKINFADHVRTIRALEVFYVTGKPLSTLKV
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHEEEEEECCCCCCEEEE
QKPPNWKILELGLDRGNLKERIFQRTKNMFLSGIIDETNHLISKYGFDLPILETIGYREA
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCHHHH
RNVLNNHSTIDKAIELTATKTIQFAKRQKTWFRNKNNPLWLNNKNPLKDAIIKIESFLS
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SSHPPHVIVLIGPTASGKTELAIEIAEYFKTNIHNIDSRQIYKSMDIGTAKPSKIQQKK
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHH
IRHFLIDIEEPINPINVKQFQEIAQKSIKEEIKKDKLPFLVGGSGLYMNSITKGFFVPDV
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEHHHCCCCCCCCCC
PPQNNLRKQLEELGQEKCWDLLENCDPLSTKKINFADHVRTIRALEVFYVTGKPLSTLKV
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHEEEEEECCCCCCEEEE
QKPPNWKILELGLDRGNLKERIFQRTKNMFLSGIIDETNHLISKYGFDLPILETIGYREA
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCHHHH
RNVLNNHSTIDKAIELTATKTIQFAKRQKTWFRNKNNPLWLNNKNPLKDAIIKIESFLS
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA