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The map label for this gene is gyrB [H]

Identifier: 126697163

GI number: 126697163

Start: 1545255

End: 1547222

Strand: Reverse

Name: gyrB [H]

Synonym: P9301_18251

Alternate gene names: 126697163

Gene position: 1547222-1545255 (Counterclockwise)

Preceding gene: 126697168

Following gene: 126697162

Centisome position: 94.23

GC content: 33.84

Gene sequence:

>1968_bases
ATGAGTGAGGACAAAAGATCTAATAAAATCTCTAATGATTATGGGGCGGAGCAAATTCAGGTTTTAGAGGGTTTAGAACC
TGTTCGTAAACGCCCTGGAATGTATATAGGTTCAACGGGACCTAGGGGATTACACCATTTAATATATGAGGTAGTTGATA
ACTCGGTTGATGAAGCACTTGCAGGACATTGTGATCATATAGAAATAGTTCTTCGAGCAGATGGTTCTGCTTTAATTTCT
GATAATGGACGAGGTATCCCAACAGATATTCATCCAAGAACAGGGAAAAGTGCCCTAGAAACTGTTCTAACTGTTCTTCA
TGCAGGAGGTAAATTTGGAAGTGGTGGTTATAAAGTTTCAGGGGGTTTGCATGGAGTAGGAATATCTGTAGTAAATGCCC
TAAGCGAATGGGTTAAGGTGACTGTTTATAGGGATGGAAGCGAATTTAATCAAAGATTTGAAAAAGGTATATCAAAGGGT
GAATTGGAAAGTAAAAAGCAGACTGGCAAATCATCTAAGAAAGGAACAACTATTTGCTTTAAACCTGACAAAACGATTTT
TTCTGGAGGAATTGAATTTGAATATGCTCTTCTTTCATCGAGATTAAGGGAACTGGCTTATCTGAATGGCGGAGTAAAAA
TTGTTTTTAGAGATGAGAGAAATCAATTATCAGATGGTTCTTTTAAAGAAGAAATTTATTTGTATCAAGGAGGTATTAAA
GAATATGTTGAATACATGAATGCTGAAAAAGAGTCTATTCATCCTGAAATAATTTATGTTGACTCACAGAAAGAAAATGT
ATATGTGGAAGCAGCTTTGCAATGGTGTTCAGATGTATATTCAGATAATATTTTGGGATTTGCAAATAATATCAGAACTA
TTGATGGAGGAACTCATATTGAAGGACTAAAAACTGTTCTGACAAGAACTTTTAATAATCTTGCAAAAAAGAGAGGTAAA
AGAAAAGATATTGAAAAAAATTTAGCTGGTGAAAATATTAGAGAGGGTTTGACTGTTGTTTTATCAGTAAAAGTTCCAGA
TCCCGAATTTGAAGGACAAACAAAAACAAAATTAGGAAATACTGAAGTACGAGGAATTGTGGATTCTCTCATTGGTGAGG
CTCTCACAAAATATATGGAATTCAATCCTGGAATTTTGGACTTGATTCTTGAAAAAGCAATTCAATCATTTAATGCAGCA
GAAGCTGCGAGAAGGGCTAGAGAATTAGTAAGAAGAAAAAGTGTTCTCGAAAGTTCTACATTACCTGGAAAATTAGCAGA
TTGTAGTTCTAGAGATCCATCAGAATCAGAAATATACATAGTTGAAGGAGATTCAGCTGGTGGCTCTGCAAAACAAGGTC
GAGATAGAAATTTTCAGGCTATTTTGCCTCTCAGGGGTAAAATTCTTAATATTGAAAAAACTGATGATACTAAAATATAT
AAAAATACAGAAATACAGTCATTAATAACAGCGCTAGGATTAGGAATAAAAGGAGAGGAGTTTGACGAAAGCTCTTTGAG
ATATCATAGAGTTGTAATTATGACGGATGCTGATGTTGACGGTGCTCATATAAGAACTTTATTGTTGACATTTTTTTATA
GATATCAAAGAGAACTTGTTGAGAAAGGTTTTATATACATTGCTTGTCCTCCTCTTTATAAAGTTGAAAGAGGAAAGAAT
CATAATTACTGTTATAACGAGAATCAATTAAAGGATACAATTCAAGGTTTTGGAGAAAATGCAAATTATAATATCCAAAG
ATTTAAGGGATTAGGTGAAATGATGCCAAAACAATTATGGGATACAACTATGAATCCTCAAACGAGGATGATGAAAAGGG
TTGAAATTGAAGATGCACTTGAAGCTGACAGAATATTTAATATTTTAATGGGAGATAAGGTTGCACCAAGAAGAGAATTT
ATTGAAACTCATAGTAGTAACTTAGATATGGCAACATTAGACATATGA

Upstream 100 bases:

>100_bases
TTCATTGATTTCCGAGCCTTTACCTTATTGCGGTGGTAAGTTTAATGAAAGACCTTTTAAAAATAACATTTTAAAAGTTT
AGTATTTTTTTTTATATTAA

Downstream 100 bases:

>100_bases
TTAATCATGTTCTCAAGAATTTTTGTTTAATTACTTTTGCATTAATTGCTCCAATTACATTACCTGCCGGTGGAATTCAA
AAAAGTTTAAATCAAAATAA

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 655; Mature: 654

Protein sequence:

>655_residues
MSEDKRSNKISNDYGAEQIQVLEGLEPVRKRPGMYIGSTGPRGLHHLIYEVVDNSVDEALAGHCDHIEIVLRADGSALIS
DNGRGIPTDIHPRTGKSALETVLTVLHAGGKFGSGGYKVSGGLHGVGISVVNALSEWVKVTVYRDGSEFNQRFEKGISKG
ELESKKQTGKSSKKGTTICFKPDKTIFSGGIEFEYALLSSRLRELAYLNGGVKIVFRDERNQLSDGSFKEEIYLYQGGIK
EYVEYMNAEKESIHPEIIYVDSQKENVYVEAALQWCSDVYSDNILGFANNIRTIDGGTHIEGLKTVLTRTFNNLAKKRGK
RKDIEKNLAGENIREGLTVVLSVKVPDPEFEGQTKTKLGNTEVRGIVDSLIGEALTKYMEFNPGILDLILEKAIQSFNAA
EAARRARELVRRKSVLESSTLPGKLADCSSRDPSESEIYIVEGDSAGGSAKQGRDRNFQAILPLRGKILNIEKTDDTKIY
KNTEIQSLITALGLGIKGEEFDESSLRYHRVVIMTDADVDGAHIRTLLLTFFYRYQRELVEKGFIYIACPPLYKVERGKN
HNYCYNENQLKDTIQGFGENANYNIQRFKGLGEMMPKQLWDTTMNPQTRMMKRVEIEDALEADRIFNILMGDKVAPRREF
IETHSSNLDMATLDI

Sequences:

>Translated_655_residues
MSEDKRSNKISNDYGAEQIQVLEGLEPVRKRPGMYIGSTGPRGLHHLIYEVVDNSVDEALAGHCDHIEIVLRADGSALIS
DNGRGIPTDIHPRTGKSALETVLTVLHAGGKFGSGGYKVSGGLHGVGISVVNALSEWVKVTVYRDGSEFNQRFEKGISKG
ELESKKQTGKSSKKGTTICFKPDKTIFSGGIEFEYALLSSRLRELAYLNGGVKIVFRDERNQLSDGSFKEEIYLYQGGIK
EYVEYMNAEKESIHPEIIYVDSQKENVYVEAALQWCSDVYSDNILGFANNIRTIDGGTHIEGLKTVLTRTFNNLAKKRGK
RKDIEKNLAGENIREGLTVVLSVKVPDPEFEGQTKTKLGNTEVRGIVDSLIGEALTKYMEFNPGILDLILEKAIQSFNAA
EAARRARELVRRKSVLESSTLPGKLADCSSRDPSESEIYIVEGDSAGGSAKQGRDRNFQAILPLRGKILNIEKTDDTKIY
KNTEIQSLITALGLGIKGEEFDESSLRYHRVVIMTDADVDGAHIRTLLLTFFYRYQRELVEKGFIYIACPPLYKVERGKN
HNYCYNENQLKDTIQGFGENANYNIQRFKGLGEMMPKQLWDTTMNPQTRMMKRVEIEDALEADRIFNILMGDKVAPRREF
IETHSSNLDMATLDI
>Mature_654_residues
SEDKRSNKISNDYGAEQIQVLEGLEPVRKRPGMYIGSTGPRGLHHLIYEVVDNSVDEALAGHCDHIEIVLRADGSALISD
NGRGIPTDIHPRTGKSALETVLTVLHAGGKFGSGGYKVSGGLHGVGISVVNALSEWVKVTVYRDGSEFNQRFEKGISKGE
LESKKQTGKSSKKGTTICFKPDKTIFSGGIEFEYALLSSRLRELAYLNGGVKIVFRDERNQLSDGSFKEEIYLYQGGIKE
YVEYMNAEKESIHPEIIYVDSQKENVYVEAALQWCSDVYSDNILGFANNIRTIDGGTHIEGLKTVLTRTFNNLAKKRGKR
KDIEKNLAGENIREGLTVVLSVKVPDPEFEGQTKTKLGNTEVRGIVDSLIGEALTKYMEFNPGILDLILEKAIQSFNAAE
AARRARELVRRKSVLESSTLPGKLADCSSRDPSESEIYIVEGDSAGGSAKQGRDRNFQAILPLRGKILNIEKTDDTKIYK
NTEIQSLITALGLGIKGEEFDESSLRYHRVVIMTDADVDGAHIRTLLLTFFYRYQRELVEKGFIYIACPPLYKVERGKNH
NYCYNENQLKDTIQGFGENANYNIQRFKGLGEMMPKQLWDTTMNPQTRMMKRVEIEDALEADRIFNILMGDKVAPRREFI
ETHSSNLDMATLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=639, Percent_Identity=24.5696400625978, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI19913406, Length=649, Percent_Identity=24.0369799691834, Blast_Score=126, Evalue=6e-29,
Organism=Escherichia coli, GI48994957, Length=610, Percent_Identity=50.1639344262295, Blast_Score=572, Evalue=1e-164,
Organism=Escherichia coli, GI1789408, Length=652, Percent_Identity=39.7239263803681, Blast_Score=439, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI212645845, Length=552, Percent_Identity=25.9057971014493, Blast_Score=129, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17535065, Length=575, Percent_Identity=23.1304347826087, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI212645657, Length=180, Percent_Identity=27.7777777777778, Blast_Score=79, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6324241, Length=629, Percent_Identity=25.5961844197138, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI17136538, Length=573, Percent_Identity=25.4799301919721, Blast_Score=129, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 73274; Mature: 73143

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEDKRSNKISNDYGAEQIQVLEGLEPVRKRPGMYIGSTGPRGLHHLIYEVVDNSVDEAL
CCCCCCCCCCCCCCCHHHHHHHHCCHHHHHCCCEEEECCCCHHHHHHHHHHHHCHHHHHH
AGHCDHIEIVLRADGSALISDNGRGIPTDIHPRTGKSALETVLTVLHAGGKFGSGGYKVS
HCCCCEEEEEEEECCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEC
GGLHGVGISVVNALSEWVKVTVYRDGSEFNQRFEKGISKGELESKKQTGKSSKKGTTICF
CCCCHHHHHHHHHHHHHEEEEEEECCHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCEEEE
KPDKTIFSGGIEFEYALLSSRLRELAYLNGGVKIVFRDERNQLSDGSFKEEIYLYQGGIK
CCCCCEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEECCHH
EYVEYMNAEKESIHPEIIYVDSQKENVYVEAALQWCSDVYSDNILGFANNIRTIDGGTHI
HHHHHHCCHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEECCCCCEECCCCCCH
EGLKTVLTRTFNNLAKKRGKRKDIEKNLAGENIREGLTVVLSVKVPDPEFEGQTKTKLGN
HHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHCCEEEEEEEECCCCCCCCCCHHCCCC
TEVRGIVDSLIGEALTKYMEFNPGILDLILEKAIQSFNAAEAARRARELVRRKSVLESST
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
LPGKLADCSSRDPSESEIYIVEGDSAGGSAKQGRDRNFQAILPLRGKILNIEKTDDTKIY
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCEE
KNTEIQSLITALGLGIKGEEFDESSLRYHRVVIMTDADVDGAHIRTLLLTFFYRYQRELV
CCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
EKGFIYIACPPLYKVERGKNHNYCYNENQLKDTIQGFGENANYNIQRFKGLGEMMPKQLW
HCCEEEEECCCCHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
DTTMNPQTRMMKRVEIEDALEADRIFNILMGDKVAPRREFIETHSSNLDMATLDI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEEECC
>Mature Secondary Structure 
SEDKRSNKISNDYGAEQIQVLEGLEPVRKRPGMYIGSTGPRGLHHLIYEVVDNSVDEAL
CCCCCCCCCCCCCCHHHHHHHHCCHHHHHCCCEEEECCCCHHHHHHHHHHHHCHHHHHH
AGHCDHIEIVLRADGSALISDNGRGIPTDIHPRTGKSALETVLTVLHAGGKFGSGGYKVS
HCCCCEEEEEEEECCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEC
GGLHGVGISVVNALSEWVKVTVYRDGSEFNQRFEKGISKGELESKKQTGKSSKKGTTICF
CCCCHHHHHHHHHHHHHEEEEEEECCHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCEEEE
KPDKTIFSGGIEFEYALLSSRLRELAYLNGGVKIVFRDERNQLSDGSFKEEIYLYQGGIK
CCCCCEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEECCHH
EYVEYMNAEKESIHPEIIYVDSQKENVYVEAALQWCSDVYSDNILGFANNIRTIDGGTHI
HHHHHHCCHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEECCCCCEECCCCCCH
EGLKTVLTRTFNNLAKKRGKRKDIEKNLAGENIREGLTVVLSVKVPDPEFEGQTKTKLGN
HHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHCCEEEEEEEECCCCCCCCCCHHCCCC
TEVRGIVDSLIGEALTKYMEFNPGILDLILEKAIQSFNAAEAARRARELVRRKSVLESST
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
LPGKLADCSSRDPSESEIYIVEGDSAGGSAKQGRDRNFQAILPLRGKILNIEKTDDTKIY
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCEE
KNTEIQSLITALGLGIKGEEFDESSLRYHRVVIMTDADVDGAHIRTLLLTFFYRYQRELV
CCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
EKGFIYIACPPLYKVERGKNHNYCYNENQLKDTIQGFGENANYNIQRFKGLGEMMPKQLW
HCCEEEEECCCCHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
DTTMNPQTRMMKRVEIEDALEADRIFNILMGDKVAPRREFIETHSSNLDMATLDI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]