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The map label for this gene is thiC

Identifier: 126697142

GI number: 126697142

Start: 1526173

End: 1527543

Strand: Reverse

Name: thiC

Synonym: P9301_18041

Alternate gene names: 126697142

Gene position: 1527543-1526173 (Counterclockwise)

Preceding gene: 126697143

Following gene: 126697138

Centisome position: 93.04

GC content: 37.64

Gene sequence:

>1371_bases
ATGAGAAGTTCTTGGATTAAGCCTCGCCTTGGGAAAGACAATGTAACTCAGATGAACTTTGCGAGAAACGGATATATCAC
CGAAGAAATGGATTTTGTTGCTAAAAAAGAGAATCTACCTCCTTCTTTAATAATGGAGGAAGTGGCAAGAGGAAGATTAA
TTATTCCAGCTAATATTAATCATTTGAATCTTGAGCCAATGTCTATAGGGGTTGCTTCTCGATGCAAAGTTAATGCCAAT
ATTGGTGCTTCTCCCAATGCAAGTGATATAAATGAAGAAGTAGAAAAGCTTAAACTAGCTGTAAAATATGGTGCTGATAC
GGTTATGGATCTTTCTACGGGAGGAGTAAATTTAGATGAAGTGCGGCAAGCAATTATTCAAGAATCTCCAGTTCCCATAG
GAACTGTTCCTGTTTATCAAGCATTAGAAAGTGTACATGGTTCAATCGATAGACTAACAGAAGACGATTTTCTTCATATT
ATTGAAAAACATTGCCAGCAAGGAGTAGATTATCAAACTATTCATGCTGGTCTATTAATAGAGCATTTACCAAAAGTTAA
AGGAAGAATCACTGGAATTGTCAGTAGAGGGGGAGGTATTTTAGCTCAATGGATGCTACATCATTTTAAGCAAAATCCCC
TCTATACAAGGTTTGATGATATCTGTGAGATTTTTAAGAAATATGATTGTACTTTTTCTCTAGGAGATTCACTAAGGCCT
GGATGTTTGCATGATGCTTCTGATGATGCTCAACTAGCTGAATTGAAGACCTTAGGTGAGCTTACTCGAAGAGCATGGGA
ACATAATGTTCAAGTAATGGTTGAGGGCCCTGGTCATGTACCTATGGACCAAATTGAGTTTAATGTGAGAAAGCAAATGG
AAGAATGTTCAGAAGCTCCTTTCTATGTACTTGGTCCATTAGTTACAGATATTTCTCCTGGTTATGACCATATTTCAAGT
GCTATTGGGGCGGCTATGGCAGGATGGTATGGAACGTCTATGTTATGTTACGTAACCCCAAAAGAACATCTAGGCCTCCC
AAATGCAGAAGATGTACGAGAAGGATTAATTGCTTATAAAATAGCCGCTCACGCTGCTGATATAGCAAGACATAGAGCTG
GTGCTCGTGATAGAGATGATGAACTTAGTCATGCAAGGTATAACTTTGATTGGAATAAACAATTCGAACTTTCTTTAGAT
CCAGAGAGGGCAAAGCAGTACCATGATGAAACACTACCTGAAGAAATCTTTAAAAAGGCTGAGTTTTGTTCAATGTGTGG
TCCAAAACATTGTCCAATGAATTCAAAGATTTCAGATGAATCTCTTGATCAGTTAAAAGATAAACTTGAAGAATGTAGTA
CTTCAGCTTAG

Upstream 100 bases:

>100_bases
AGCTGAGATCATACCCTTTGAACCTGAATCATTAATTTTGAAGCAGGGAAGTGGAGATTTGATCAAACTTTAGTAATAAT
ACTTTTAAAATTAGGAAATT

Downstream 100 bases:

>100_bases
TTATCAATTATTAATAGTTATAGAATTTCCTTCGTCTTATTAACTACGTTTTCGACTGTAAATCCAAAATTTTTCATACA
TTCTCCACCTGGTGCTGATG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MRSSWIKPRLGKDNVTQMNFARNGYITEEMDFVAKKENLPPSLIMEEVARGRLIIPANINHLNLEPMSIGVASRCKVNAN
IGASPNASDINEEVEKLKLAVKYGADTVMDLSTGGVNLDEVRQAIIQESPVPIGTVPVYQALESVHGSIDRLTEDDFLHI
IEKHCQQGVDYQTIHAGLLIEHLPKVKGRITGIVSRGGGILAQWMLHHFKQNPLYTRFDDICEIFKKYDCTFSLGDSLRP
GCLHDASDDAQLAELKTLGELTRRAWEHNVQVMVEGPGHVPMDQIEFNVRKQMEECSEAPFYVLGPLVTDISPGYDHISS
AIGAAMAGWYGTSMLCYVTPKEHLGLPNAEDVREGLIAYKIAAHAADIARHRAGARDRDDELSHARYNFDWNKQFELSLD
PERAKQYHDETLPEEIFKKAEFCSMCGPKHCPMNSKISDESLDQLKDKLEECSTSA

Sequences:

>Translated_456_residues
MRSSWIKPRLGKDNVTQMNFARNGYITEEMDFVAKKENLPPSLIMEEVARGRLIIPANINHLNLEPMSIGVASRCKVNAN
IGASPNASDINEEVEKLKLAVKYGADTVMDLSTGGVNLDEVRQAIIQESPVPIGTVPVYQALESVHGSIDRLTEDDFLHI
IEKHCQQGVDYQTIHAGLLIEHLPKVKGRITGIVSRGGGILAQWMLHHFKQNPLYTRFDDICEIFKKYDCTFSLGDSLRP
GCLHDASDDAQLAELKTLGELTRRAWEHNVQVMVEGPGHVPMDQIEFNVRKQMEECSEAPFYVLGPLVTDISPGYDHISS
AIGAAMAGWYGTSMLCYVTPKEHLGLPNAEDVREGLIAYKIAAHAADIARHRAGARDRDDELSHARYNFDWNKQFELSLD
PERAKQYHDETLPEEIFKKAEFCSMCGPKHCPMNSKISDESLDQLKDKLEECSTSA
>Mature_456_residues
MRSSWIKPRLGKDNVTQMNFARNGYITEEMDFVAKKENLPPSLIMEEVARGRLIIPANINHLNLEPMSIGVASRCKVNAN
IGASPNASDINEEVEKLKLAVKYGADTVMDLSTGGVNLDEVRQAIIQESPVPIGTVPVYQALESVHGSIDRLTEDDFLHI
IEKHCQQGVDYQTIHAGLLIEHLPKVKGRITGIVSRGGGILAQWMLHHFKQNPLYTRFDDICEIFKKYDCTFSLGDSLRP
GCLHDASDDAQLAELKTLGELTRRAWEHNVQVMVEGPGHVPMDQIEFNVRKQMEECSEAPFYVLGPLVTDISPGYDHISS
AIGAAMAGWYGTSMLCYVTPKEHLGLPNAEDVREGLIAYKIAAHAADIARHRAGARDRDDELSHARYNFDWNKQFELSLD
PERAKQYHDETLPEEIFKKAEFCSMCGPKHCPMNSKISDESLDQLKDKLEECSTSA

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=470, Percent_Identity=55.7446808510638, Blast_Score=514, Evalue=1e-147,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_PROM0 (A3PFA2)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001092028.1
- STRING:   A3PFA2
- GeneID:   4911553
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_18041
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   EPMAIGI
- ProtClustDB:   PRK13352
- BioCyc:   PMAR167546:P9301ORF_1842-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 50972; Mature: 50972

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: BINDING 80-80 BINDING 109-109 BINDING 139-139 BINDING 175-175 BINDING 275-275 BINDING 302-302

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSSWIKPRLGKDNVTQMNFARNGYITEEMDFVAKKENLPPSLIMEEVARGRLIIPANIN
CCCCCCCCCCCCCCCEEEHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCC
HLNLEPMSIGVASRCKVNANIGASPNASDINEEVEKLKLAVKYGADTVMDLSTGGVNLDE
EEECCHHEECCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHH
VRQAIIQESPVPIGTVPVYQALESVHGSIDRLTEDDFLHIIEKHCQQGVDYQTIHAGLLI
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
EHLPKVKGRITGIVSRGGGILAQWMLHHFKQNPLYTRFDDICEIFKKYDCTFSLGDSLRP
HHHHHHHHHHEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEECCCCCCC
GCLHDASDDAQLAELKTLGELTRRAWEHNVQVMVEGPGHVPMDQIEFNVRKQMEECSEAP
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
FYVLGPLVTDISPGYDHISSAIGAAMAGWYGTSMLCYVTPKEHLGLPNAEDVREGLIAYK
EEEECHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCHHHHHHHHHHHH
IAAHAADIARHRAGARDRDDELSHARYNFDWNKQFELSLDPERAKQYHDETLPEEIFKKA
HHHHHHHHHHHHCCCCCCCCHHHHCEECCCCCCEEEEEECHHHHHHHHHCCCHHHHHHHH
EFCSMCGPKHCPMNSKISDESLDQLKDKLEECSTSA
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRSSWIKPRLGKDNVTQMNFARNGYITEEMDFVAKKENLPPSLIMEEVARGRLIIPANIN
CCCCCCCCCCCCCCCEEEHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCC
HLNLEPMSIGVASRCKVNANIGASPNASDINEEVEKLKLAVKYGADTVMDLSTGGVNLDE
EEECCHHEECCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHH
VRQAIIQESPVPIGTVPVYQALESVHGSIDRLTEDDFLHIIEKHCQQGVDYQTIHAGLLI
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
EHLPKVKGRITGIVSRGGGILAQWMLHHFKQNPLYTRFDDICEIFKKYDCTFSLGDSLRP
HHHHHHHHHHEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEECCCCCCC
GCLHDASDDAQLAELKTLGELTRRAWEHNVQVMVEGPGHVPMDQIEFNVRKQMEECSEAP
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
FYVLGPLVTDISPGYDHISSAIGAAMAGWYGTSMLCYVTPKEHLGLPNAEDVREGLIAYK
EEEECHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCHHHHHHHHHHHH
IAAHAADIARHRAGARDRDDELSHARYNFDWNKQFELSLDPERAKQYHDETLPEEIFKKA
HHHHHHHHHHHHCCCCCCCCHHHHCEECCCCCCEEEEEECHHHHHHHHHCCCHHHHHHHH
EFCSMCGPKHCPMNSKISDESLDQLKDKLEECSTSA
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA