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The map label for this gene is kefB [H]

Identifier: 126697130

GI number: 126697130

Start: 1513248

End: 1514615

Strand: Reverse

Name: kefB [H]

Synonym: P9301_17921

Alternate gene names: 126697130

Gene position: 1514615-1513248 (Counterclockwise)

Preceding gene: 126697132

Following gene: 126697129

Centisome position: 92.25

GC content: 38.52

Gene sequence:

>1368_bases
ATGTATCCTTTACTTGCTGAATTAAGTGCACATGATTTAGAAGTTGCTGAAACTTTGATAGGAGTTATAAGGTTTCTACT
GATATTTTTAGCTGCAAGAGCATTAGCAGAAGTATTAGTAAGACTGAGTTTACCAACGATAGTAGGTGAGCTGCTTGCAG
GGGTTGTAATAGGAGCATCAGGATTCCATTTGTTGATACCGCCCTCGGCAGGAACCGAATTAAATGAAGGACTTGTAAAT
GTTATTAGCTCGTTGGCGTCAATCCCCCCAGAAGCAGTACCTGATGTTTATTTCGAAAGTTTTCCATCCCTCCAAGCAGT
AGCAACTCTAGGATTATATGCTCTTTTATTTTTGACAGGATTAGAAAGTGAGTTAGAGGAATTAGTAGCTGTAGGAGCTC
AAGCTTTTACAGTTGCTATGGCTGGTGTAATTTTACCTTTTGCCTTTGGAACTCTTGGATTAATGTTTATTTTCCAGGTA
GATCTAATTCCAGCAGTTTTTGCTGGAGCATCTATGACAGCAACAAGTATAGGAATTACTGCTAGTGTTTTTGGTGAGTT
GGGATATTTAAAAACCAGAGAAGGACAAATTGTTATTGGTGCAGCAGTATTAGACGATATTTTGGGAATTGTAATTCTGG
CTGTTGTTGTGGCTCTTGCTGCAGGAGGTACTTTAGAAATTGCTCCTATTGTTAAATTAGTTGCTGCAGCTGTAGTATTT
GTTATTGCTGCTATCGCATTAAGTAGAACAGCAGCCCCAGGGTTTGATTGGTTATTAGATAGATTAAAGGCTCCTGGAGC
TGTAGTGGTAGCTTCTTTTGTGATACTTGTGCTGTGTTGTTTTGTCGCAACAGCCATTGGATTGGAAGCAGCTTTAGGTG
CTTTTGCAGCTGGACTAATTCTTAGTAGTTCTAAAAATAATCACGCAATTCAACAATCCGTTTTACCTTTAGTATCGTTA
TTCGCAACTATTTTCTTCGTATTAGTTGGAGCTGGAATGGATTTATCAGTTATTAATCCACTTGATCCAACAAGTAGGTC
AGCTCTTGTAGTTGCAGGATTTTTATTAGTTGTAGCAATTATTGGAAAAATTGCTGCCGGATGGGTATTTTCGAGTGATA
AACCTACTAATCGATTAGTTGTAGGTTTGGGCATGATGCCTAGAGGAGAGGTTGGTTTAATTTTCCTTGGGCTAGGAACA
AGCGCTAAGTTGTTAACTCCTTCTCTTGAAGCAGCTATTTTATTAATGGTTATTGGAACTACATTTCTTGCACCTGTTCT
CTTGAGAATTGTTCTAAAAGATAAGCCACCAAATGATGGCAATAAAATTTCAGATGATGTTGCTGCTGATCCTGTAGGTC
TTCTTTAG

Upstream 100 bases:

>100_bases
CACTTGTTGAGTATTTTTGCTTTTTTTTTACATATTTCGTAAAGTGGGCCCTCTATAAACTATCTTCCAATTATGTAGTT
TATTTTTTCTCTCATTTCAT

Downstream 100 bases:

>100_bases
GAAATAATTTTATTAGTATTAACTAATATAATATTTTCATTAAATGGAAAAATCAGCTCTGATAGATAGTGATGTAAATT
ATGACTGGAATTTTTTAAAT

Product: CPA2 family Na+/H+ antiporter

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MYPLLAELSAHDLEVAETLIGVIRFLLIFLAARALAEVLVRLSLPTIVGELLAGVVIGASGFHLLIPPSAGTELNEGLVN
VISSLASIPPEAVPDVYFESFPSLQAVATLGLYALLFLTGLESELEELVAVGAQAFTVAMAGVILPFAFGTLGLMFIFQV
DLIPAVFAGASMTATSIGITASVFGELGYLKTREGQIVIGAAVLDDILGIVILAVVVALAAGGTLEIAPIVKLVAAAVVF
VIAAIALSRTAAPGFDWLLDRLKAPGAVVVASFVILVLCCFVATAIGLEAALGAFAAGLILSSSKNNHAIQQSVLPLVSL
FATIFFVLVGAGMDLSVINPLDPTSRSALVVAGFLLVVAIIGKIAAGWVFSSDKPTNRLVVGLGMMPRGEVGLIFLGLGT
SAKLLTPSLEAAILLMVIGTTFLAPVLLRIVLKDKPPNDGNKISDDVAADPVGLL

Sequences:

>Translated_455_residues
MYPLLAELSAHDLEVAETLIGVIRFLLIFLAARALAEVLVRLSLPTIVGELLAGVVIGASGFHLLIPPSAGTELNEGLVN
VISSLASIPPEAVPDVYFESFPSLQAVATLGLYALLFLTGLESELEELVAVGAQAFTVAMAGVILPFAFGTLGLMFIFQV
DLIPAVFAGASMTATSIGITASVFGELGYLKTREGQIVIGAAVLDDILGIVILAVVVALAAGGTLEIAPIVKLVAAAVVF
VIAAIALSRTAAPGFDWLLDRLKAPGAVVVASFVILVLCCFVATAIGLEAALGAFAAGLILSSSKNNHAIQQSVLPLVSL
FATIFFVLVGAGMDLSVINPLDPTSRSALVVAGFLLVVAIIGKIAAGWVFSSDKPTNRLVVGLGMMPRGEVGLIFLGLGT
SAKLLTPSLEAAILLMVIGTTFLAPVLLRIVLKDKPPNDGNKISDDVAADPVGLL
>Mature_455_residues
MYPLLAELSAHDLEVAETLIGVIRFLLIFLAARALAEVLVRLSLPTIVGELLAGVVIGASGFHLLIPPSAGTELNEGLVN
VISSLASIPPEAVPDVYFESFPSLQAVATLGLYALLFLTGLESELEELVAVGAQAFTVAMAGVILPFAFGTLGLMFIFQV
DLIPAVFAGASMTATSIGITASVFGELGYLKTREGQIVIGAAVLDDILGIVILAVVVALAAGGTLEIAPIVKLVAAAVVF
VIAAIALSRTAAPGFDWLLDRLKAPGAVVVASFVILVLCCFVATAIGLEAALGAFAAGLILSSSKNNHAIQQSVLPLVSL
FATIFFVLVGAGMDLSVINPLDPTSRSALVVAGFLLVVAIIGKIAAGWVFSSDKPTNRLVVGLGMMPRGEVGLIFLGLGT
SAKLLTPSLEAAILLMVIGTTFLAPVLLRIVLKDKPPNDGNKISDDVAADPVGLL

Specific function: This is a Na(+)/H(+) antiporter. It can mediate sodium uptake when a transmembrane pH gradient is applied [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family [H]

Homologues:

Organism=Escherichia coli, GI1786232, Length=447, Percent_Identity=24.8322147651007, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786685, Length=345, Percent_Identity=27.536231884058, Blast_Score=62, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6322367, Length=453, Percent_Identity=22.9580573951435, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR004771 [H]

Pfam domain/function: PF00999 Na_H_Exchanger [H]

EC number: NA

Molecular weight: Translated: 46763; Mature: 46763

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYPLLAELSAHDLEVAETLIGVIRFLLIFLAARALAEVLVRLSLPTIVGELLAGVVIGAS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GFHLLIPPSAGTELNEGLVNVISSLASIPPEAVPDVYFESFPSLQAVATLGLYALLFLTG
CCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHC
LESELEELVAVGAQAFTVAMAGVILPFAFGTLGLMFIFQVDLIPAVFAGASMTATSIGIT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
ASVFGELGYLKTREGQIVIGAAVLDDILGIVILAVVVALAAGGTLEIAPIVKLVAAAVVF
HHHHHHHCCCEECCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VIAAIALSRTAAPGFDWLLDRLKAPGAVVVASFVILVLCCFVATAIGLEAALGAFAAGLI
HHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSSSKNNHAIQQSVLPLVSLFATIFFVLVGAGMDLSVINPLDPTSRSALVVAGFLLVVAI
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHH
IGKIAAGWVFSSDKPTNRLVVGLGMMPRGEVGLIFLGLGTSAKLLTPSLEAAILLMVIGT
HHHHHHCCEECCCCCCCEEEEEECCCCCCCCCEEEEECCCCCHHCCCHHHHHHHHHHHHH
TFLAPVLLRIVLKDKPPNDGNKISDDVAADPVGLL
HHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCC
>Mature Secondary Structure
MYPLLAELSAHDLEVAETLIGVIRFLLIFLAARALAEVLVRLSLPTIVGELLAGVVIGAS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GFHLLIPPSAGTELNEGLVNVISSLASIPPEAVPDVYFESFPSLQAVATLGLYALLFLTG
CCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHC
LESELEELVAVGAQAFTVAMAGVILPFAFGTLGLMFIFQVDLIPAVFAGASMTATSIGIT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
ASVFGELGYLKTREGQIVIGAAVLDDILGIVILAVVVALAAGGTLEIAPIVKLVAAAVVF
HHHHHHHCCCEECCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VIAAIALSRTAAPGFDWLLDRLKAPGAVVVASFVILVLCCFVATAIGLEAALGAFAAGLI
HHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSSSKNNHAIQQSVLPLVSLFATIFFVLVGAGMDLSVINPLDPTSRSALVVAGFLLVVAI
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHH
IGKIAAGWVFSSDKPTNRLVVGLGMMPRGEVGLIFLGLGTSAKLLTPSLEAAILLMVIGT
HHHHHHCCEECCCCCCCEEEEEECCCCCCCCCEEEEECCCCCHHCCCHHHHHHHHHHHHH
TFLAPVLLRIVLKDKPPNDGNKISDDVAADPVGLL
HHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1312090; 1325937 [H]