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The map label for this gene is trpE [H]

Identifier: 126697106

GI number: 126697106

Start: 1491404

End: 1492924

Strand: Reverse

Name: trpE [H]

Synonym: P9301_17681

Alternate gene names: 126697106

Gene position: 1492924-1491404 (Counterclockwise)

Preceding gene: 126697107

Following gene: 126697105

Centisome position: 90.93

GC content: 33.6

Gene sequence:

>1521_bases
ATGATCAGCTCACAGAAAATTAGTTTTTTAAAGGCTTATAAAGAAGGTAAAAATTTTATACCTATAGTAGAAACTTGGCC
AGCTGATTTGGAGACTCCATTATCAACTTGGTTAAAATTATCTTCAAAAGATTCCCATGGTGTTTTTCTTGAATCTGTTG
AAGGTGGGGAGAATTTGGGCAGGTGGAGTATTGTTGCTACTAAACCTCTTTGGGAGGCAGTTTGTTATGGAGAAGAAATA
GTTAAAACTTGGAATAATGGCAAAACTGAAACACATAAAGGTGATCCTTTTGATATTTTAAAAAGTTGGACAATGGAATA
TAAGTCAACCATGCTTGAAGAATTACCATCAATTGGACAGTTATATGGCTCTTGGGGTTATGAATTAATAAATCGAATAG
AGCCAAGCGTTCCAATAAATGAAATGCCAGAAAACAATATCCCTCAAGGTTCCTGGATGTTTTTTGATCAATTAGTTGTT
TTTGATCAAATAAAAAGATGTATTACTGCGGTGGTTTATGCAGATACAACTTCTTCTCAAGAGTCTTCAATTGAAGAGTT
GTATCTAAACTCAATATCTAAAATTCAGGAAACTAGAAATTTAATGAGAGTTCCTCTAAAAGAAAATGAGTTTTTAGATT
GGAATGAAAATGATAATTTAAATTTAGATTTAGAAAGTAATTGGGAGAAAAAAGATTTTGAGGATGCAGTTCTCTCTGCA
AAAGAATACATAAGAAAGGGAGATATCTTCCAAATAGTTATAAGTCAAAGATTTCAAACTCAAGTCAATAATGATCCCTT
TAACTTATATAGAAGTCTGAGGATGGTTAATCCATCTCCTTACATGTCATTTTTTGATTTCGGCTCATGGTATCTGATAG
GTTCAAGTCCTGAAGTAATGGTTAAAGCAGAAAAAAATAAAAATAGTCAGATTGTTGCAAGCTTAAGACCAATAGCTGGC
ACGAGACCAAGAGGTATTGATAATCAACAAGACTTGGAATTAGAAAAGGAATTATTAAAGGATCCCAAAGAGATAGCTGA
GCATGTAATGTTGATTGATCTTGGAAGAAATGATCTTGGAAGAGTTTGTGAAATTGGTACTGTCAAGGTAAAGGATTTAA
TGATTATTGAGAAATATTCACATGTTATGCATATAGTCAGTCAAGTTGAGGGAATCTTAAAAAATAATGCTGATGTATGG
GATTTGCTCAAAGCATCCTTTCCTGCTGGGACTGTGACTGGCGCCCCAAAAATAAGAGCTATGCAATTGATTAAGCATTT
TGAAAAAGATGCTAGAGGACCTTATGCTGGTGTATACGGATCTATTGATATTAATGGTGCATTAAATACAGCAATTACGA
TAAGAACCATGATAGTTAAACCCTCAAGAGATGGGAAATATGATGTGTCAGTGCAAGCAGGAGCTGGAATAGTTGCTGAT
TCTTTTCCTGAAAATGAATATCAAGAGACGATAAATAAAGCAAAGGGAATACTTAAAGCATTAGCCTGTTTGGATAAATA
A

Upstream 100 bases:

>100_bases
CTGCTGGTTTGAAATTTACAACTAAGAATACTTTTGATTAACTTCCTTAAAGTTGATATAAAAGAAGAAAATAATTATAA
TTTGGGCTGACATTATTAAT

Downstream 100 bases:

>100_bases
ATGAGTCAAAATAATCTTTATAAAGGTTTTGAGGTGGAACTTTTCACAGGTTCTTTAAATTCTCATATTGGTGTTTCGGC
TGAAATTGAGAAAAAATTTC

Product: anthranilate synthase component I/chorismate-binding protein

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MISSQKISFLKAYKEGKNFIPIVETWPADLETPLSTWLKLSSKDSHGVFLESVEGGENLGRWSIVATKPLWEAVCYGEEI
VKTWNNGKTETHKGDPFDILKSWTMEYKSTMLEELPSIGQLYGSWGYELINRIEPSVPINEMPENNIPQGSWMFFDQLVV
FDQIKRCITAVVYADTTSSQESSIEELYLNSISKIQETRNLMRVPLKENEFLDWNENDNLNLDLESNWEKKDFEDAVLSA
KEYIRKGDIFQIVISQRFQTQVNNDPFNLYRSLRMVNPSPYMSFFDFGSWYLIGSSPEVMVKAEKNKNSQIVASLRPIAG
TRPRGIDNQQDLELEKELLKDPKEIAEHVMLIDLGRNDLGRVCEIGTVKVKDLMIIEKYSHVMHIVSQVEGILKNNADVW
DLLKASFPAGTVTGAPKIRAMQLIKHFEKDARGPYAGVYGSIDINGALNTAITIRTMIVKPSRDGKYDVSVQAGAGIVAD
SFPENEYQETINKAKGILKALACLDK

Sequences:

>Translated_506_residues
MISSQKISFLKAYKEGKNFIPIVETWPADLETPLSTWLKLSSKDSHGVFLESVEGGENLGRWSIVATKPLWEAVCYGEEI
VKTWNNGKTETHKGDPFDILKSWTMEYKSTMLEELPSIGQLYGSWGYELINRIEPSVPINEMPENNIPQGSWMFFDQLVV
FDQIKRCITAVVYADTTSSQESSIEELYLNSISKIQETRNLMRVPLKENEFLDWNENDNLNLDLESNWEKKDFEDAVLSA
KEYIRKGDIFQIVISQRFQTQVNNDPFNLYRSLRMVNPSPYMSFFDFGSWYLIGSSPEVMVKAEKNKNSQIVASLRPIAG
TRPRGIDNQQDLELEKELLKDPKEIAEHVMLIDLGRNDLGRVCEIGTVKVKDLMIIEKYSHVMHIVSQVEGILKNNADVW
DLLKASFPAGTVTGAPKIRAMQLIKHFEKDARGPYAGVYGSIDINGALNTAITIRTMIVKPSRDGKYDVSVQAGAGIVAD
SFPENEYQETINKAKGILKALACLDK
>Mature_506_residues
MISSQKISFLKAYKEGKNFIPIVETWPADLETPLSTWLKLSSKDSHGVFLESVEGGENLGRWSIVATKPLWEAVCYGEEI
VKTWNNGKTETHKGDPFDILKSWTMEYKSTMLEELPSIGQLYGSWGYELINRIEPSVPINEMPENNIPQGSWMFFDQLVV
FDQIKRCITAVVYADTTSSQESSIEELYLNSISKIQETRNLMRVPLKENEFLDWNENDNLNLDLESNWEKKDFEDAVLSA
KEYIRKGDIFQIVISQRFQTQVNNDPFNLYRSLRMVNPSPYMSFFDFGSWYLIGSSPEVMVKAEKNKNSQIVASLRPIAG
TRPRGIDNQQDLELEKELLKDPKEIAEHVMLIDLGRNDLGRVCEIGTVKVKDLMIIEKYSHVMHIVSQVEGILKNNADVW
DLLKASFPAGTVTGAPKIRAMQLIKHFEKDARGPYAGVYGSIDINGALNTAITIRTMIVKPSRDGKYDVSVQAGAGIVAD
SFPENEYQETINKAKGILKALACLDK

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=391, Percent_Identity=35.8056265984655, Blast_Score=222, Evalue=5e-59,
Organism=Escherichia coli, GI1788114, Length=467, Percent_Identity=31.2633832976445, Blast_Score=215, Evalue=4e-57,
Organism=Escherichia coli, GI1786809, Length=276, Percent_Identity=26.0869565217391, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI87082077, Length=289, Percent_Identity=25.2595155709343, Blast_Score=63, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6320935, Length=492, Percent_Identity=34.9593495934959, Blast_Score=262, Evalue=8e-71,
Organism=Saccharomyces cerevisiae, GI6324361, Length=416, Percent_Identity=26.9230769230769, Blast_Score=125, Evalue=2e-29,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 57317; Mature: 57317

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISSQKISFLKAYKEGKNFIPIVETWPADLETPLSTWLKLSSKDSHGVFLESVEGGENLG
CCCCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHEECCCCCCCEEEEECCCCCCCC
RWSIVATKPLWEAVCYGEEIVKTWNNGKTETHKGDPFDILKSWTMEYKSTMLEELPSIGQ
CEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LYGSWGYELINRIEPSVPINEMPENNIPQGSWMFFDQLVVFDQIKRCITAVVYADTTSSQ
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCH
ESSIEELYLNSISKIQETRNLMRVPLKENEFLDWNENDNLNLDLESNWEKKDFEDAVLSA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHH
KEYIRKGDIFQIVISQRFQTQVNNDPFNLYRSLRMVNPSPYMSFFDFGSWYLIGSSPEVM
HHHHHCCCEEEEHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHCCCCEEEECCCCCEE
VKAEKNKNSQIVASLRPIAGTRPRGIDNQQDLELEKELLKDPKEIAEHVMLIDLGRNDLG
EEECCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHEEEEECCCCCHH
RVCEIGTVKVKDLMIIEKYSHVMHIVSQVEGILKNNADVWDLLKASFPAGTVTGAPKIRA
HHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHH
MQLIKHFEKDARGPYAGVYGSIDINGALNTAITIRTMIVKPSRDGKYDVSVQAGAGIVAD
HHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEEEEEEEEECCCCCCEEEEEECCCCEEEC
SFPENEYQETINKAKGILKALACLDK
CCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MISSQKISFLKAYKEGKNFIPIVETWPADLETPLSTWLKLSSKDSHGVFLESVEGGENLG
CCCCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHEECCCCCCCEEEEECCCCCCCC
RWSIVATKPLWEAVCYGEEIVKTWNNGKTETHKGDPFDILKSWTMEYKSTMLEELPSIGQ
CEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LYGSWGYELINRIEPSVPINEMPENNIPQGSWMFFDQLVVFDQIKRCITAVVYADTTSSQ
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCH
ESSIEELYLNSISKIQETRNLMRVPLKENEFLDWNENDNLNLDLESNWEKKDFEDAVLSA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHH
KEYIRKGDIFQIVISQRFQTQVNNDPFNLYRSLRMVNPSPYMSFFDFGSWYLIGSSPEVM
HHHHHCCCEEEEHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHCCCCEEEECCCCCEE
VKAEKNKNSQIVASLRPIAGTRPRGIDNQQDLELEKELLKDPKEIAEHVMLIDLGRNDLG
EEECCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHEEEEECCCCCHH
RVCEIGTVKVKDLMIIEKYSHVMHIVSQVEGILKNNADVWDLLKASFPAGTVTGAPKIRA
HHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHH
MQLIKHFEKDARGPYAGVYGSIDINGALNTAITIRTMIVKPSRDGKYDVSVQAGAGIVAD
HHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEEEEEEEEECCCCCCEEEEEECCCCEEEC
SFPENEYQETINKAKGILKALACLDK
CCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231; 3141423 [H]