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The map label for this gene is rpoA

Identifier: 126697065

GI number: 126697065

Start: 1459618

End: 1460556

Strand: Reverse

Name: rpoA

Synonym: P9301_17271

Alternate gene names: 126697065

Gene position: 1460556-1459618 (Counterclockwise)

Preceding gene: 126697066

Following gene: 126697064

Centisome position: 88.96

GC content: 41.11

Gene sequence:

>939_bases
GTGTTGCAATACCAGATTGAAAGAATCGACCATCAAATAGCAGATGATCGCTCCCAAACAGGAACTTTTTTAATTGGCCC
TCTAGAAAGGGGACAAGCTACAACTTTGGGTAATTCTCTTAGAAGAGTCCTTATGGGAGGACTTGAAGGGAGTGCAGTTA
CTGCAGTAAGAATAGCAGGAATTAATCATGAATATGCCACTATTCCTGGAGTTAGAGAAGACGTTTTAGATATTCTTCTT
AATTGCAAGCAACTATCAATAAATAGCACTAATCCAGAGCTCGAAATCGGCAGGTTAGTAGCTAGTGGTCCAATGGAGGT
GAAGGCTAATGACATTCAATTCTCCTCTCAAGTTGAAATTGTTGACGGCGAAAAACCGATTGCGACTATTCAGGAGGGTC
ATAACTTAGAGTTGGAAATCCATGTTGAAAGAGGCGTTGGATATAGACCCGTCGACCGAAAGAGTGAAGAGACAACTGCC
ATTGATTTGCTTCAAATAGATGCTGTATTTATGCCGGTGAAGAGAGTGAACTTTACGATTGATGAAACTGCTGTAGCAGA
AGGCGGAACGGGAAGAGAAAGATTAAAAATGGAAGTAGTAACAGATGGCTCAACCAGTCCTGACGATGCTATTGCTGAAG
CTGCAAACCAATTAATAGAACTCTTCCAACCCCTCGCTACTGTAACAATGGTTGAAGAAATCCCTGAAGAACCTGAACCA
TCTCCTGAAGCTCAAATTCCTCTCGAGGAACTAAACTTGTCCGTTAGAGCATATAATTGTTTGAAACGGGCGCAAGTTAA
CTCAGTTTCAGATTTAATGGGCTTTAGCTATGAAGATCTTCTTGAAATTAAGAACTTTGGCTCTAAATCTGCAGATGAGG
TGATTGAAGCTCTTGAGCGCATTGGCATTTCTATTCCACAAAGCAGAACATCTGTTTAA

Upstream 100 bases:

>100_bases
CTCCATTACCTCATAATGGATGTAGAAGACCTAAACGGAGACGCGTTTAGATCTTAACCATTCTCACCCCCCTAAAAACC
TCTTAACCTCATTATTTTCC

Downstream 100 bases:

>100_bases
CAATTTTTAAGATTATGAGACACCAACTTAGAATTCCATTATTAAGTAAACCTGCTGATCAGAGGAAAGCACTTCTGAGA
GGTTTAACTACACAATTAAT

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MLQYQIERIDHQIADDRSQTGTFLIGPLERGQATTLGNSLRRVLMGGLEGSAVTAVRIAGINHEYATIPGVREDVLDILL
NCKQLSINSTNPELEIGRLVASGPMEVKANDIQFSSQVEIVDGEKPIATIQEGHNLELEIHVERGVGYRPVDRKSEETTA
IDLLQIDAVFMPVKRVNFTIDETAVAEGGTGRERLKMEVVTDGSTSPDDAIAEAANQLIELFQPLATVTMVEEIPEEPEP
SPEAQIPLEELNLSVRAYNCLKRAQVNSVSDLMGFSYEDLLEIKNFGSKSADEVIEALERIGISIPQSRTSV

Sequences:

>Translated_312_residues
MLQYQIERIDHQIADDRSQTGTFLIGPLERGQATTLGNSLRRVLMGGLEGSAVTAVRIAGINHEYATIPGVREDVLDILL
NCKQLSINSTNPELEIGRLVASGPMEVKANDIQFSSQVEIVDGEKPIATIQEGHNLELEIHVERGVGYRPVDRKSEETTA
IDLLQIDAVFMPVKRVNFTIDETAVAEGGTGRERLKMEVVTDGSTSPDDAIAEAANQLIELFQPLATVTMVEEIPEEPEP
SPEAQIPLEELNLSVRAYNCLKRAQVNSVSDLMGFSYEDLLEIKNFGSKSADEVIEALERIGISIPQSRTSV
>Mature_312_residues
MLQYQIERIDHQIADDRSQTGTFLIGPLERGQATTLGNSLRRVLMGGLEGSAVTAVRIAGINHEYATIPGVREDVLDILL
NCKQLSINSTNPELEIGRLVASGPMEVKANDIQFSSQVEIVDGEKPIATIQEGHNLELEIHVERGVGYRPVDRKSEETTA
IDLLQIDAVFMPVKRVNFTIDETAVAEGGTGRERLKMEVVTDGSTSPDDAIAEAANQLIELFQPLATVTMVEEIPEEPEP
SPEAQIPLEELNLSVRAYNCLKRAQVNSVSDLMGFSYEDLLEIKNFGSKSADEVIEALERIGISIPQSRTSV

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=286, Percent_Identity=37.7622377622378, Blast_Score=162, Evalue=3e-41,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_PROM0 (A3PF25)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001091951.1
- ProteinModelPortal:   A3PF25
- SMR:   A3PF25
- STRING:   A3PF25
- GeneID:   4912388
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_17271
- eggNOG:   COG0202
- HOGENOM:   HBG430844
- OMA:   EKPIATI
- ProtClustDB:   PRK05182
- BioCyc:   PMAR167546:P9301ORF_1760-MONOMER
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR003583
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00278
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 34194; Mature: 34194

Theoretical pI: Translated: 4.21; Mature: 4.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQYQIERIDHQIADDRSQTGTFLIGPLERGQATTLGNSLRRVLMGGLEGSAVTAVRIAG
CCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEC
INHEYATIPGVREDVLDILLNCKQLSINSTNPELEIGRLVASGPMEVKANDIQFSSQVEI
CCCCCCCCCCCHHHHHHHHHCCHHEEECCCCCCEEEEEEEECCCCEEEECCEEECCEEEE
VDGEKPIATIQEGHNLELEIHVERGVGYRPVDRKSEETTAIDLLQIDAVFMPVKRVNFTI
ECCCCCEEECCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHEEHHHHHCCHHHEEEEE
DETAVAEGGTGRERLKMEVVTDGSTSPDDAIAEAANQLIELFQPLATVTMVEEIPEEPEP
CHHHHCCCCCCHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SPEAQIPLEELNLSVRAYNCLKRAQVNSVSDLMGFSYEDLLEIKNFGSKSADEVIEALER
CCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHH
IGISIPQSRTSV
CCCCCCCCCCCC
>Mature Secondary Structure
MLQYQIERIDHQIADDRSQTGTFLIGPLERGQATTLGNSLRRVLMGGLEGSAVTAVRIAG
CCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEC
INHEYATIPGVREDVLDILLNCKQLSINSTNPELEIGRLVASGPMEVKANDIQFSSQVEI
CCCCCCCCCCCHHHHHHHHHCCHHEEECCCCCCEEEEEEEECCCCEEEECCEEECCEEEE
VDGEKPIATIQEGHNLELEIHVERGVGYRPVDRKSEETTAIDLLQIDAVFMPVKRVNFTI
ECCCCCEEECCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHEEHHHHHCCHHHEEEEE
DETAVAEGGTGRERLKMEVVTDGSTSPDDAIAEAANQLIELFQPLATVTMVEEIPEEPEP
CHHHHCCCCCCHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SPEAQIPLEELNLSVRAYNCLKRAQVNSVSDLMGFSYEDLLEIKNFGSKSADEVIEALER
CCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHH
IGISIPQSRTSV
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA