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The map label for this gene is truA

Identifier: 126697063

GI number: 126697063

Start: 1458408

End: 1459214

Strand: Reverse

Name: truA

Synonym: P9301_17251

Alternate gene names: 126697063

Gene position: 1459214-1458408 (Counterclockwise)

Preceding gene: 126697064

Following gene: 126697062

Centisome position: 88.87

GC content: 30.73

Gene sequence:

>807_bases
TTGAAAAGGGTAGCTTTACTAGTCCAATATGATGGATCTCATTATTCAGGTTGGCAAAAACAAAAAAATGCAACTACTGT
TCAAGAAATTTTAGACAGAGCTCTCTTAAAGATTACAAATCATACAGTGCAGACTTTTGCAGCAGGGAGGACTGATGCTG
GTGTTCATGCATCAGGTCAAGTAGTACACTGTGATGTTGATTGTGTTGTTCCAGGAAATAGTTATTCTGATGTCTTAAAT
AGTCTTTTACCCTCATCAATTAGGATCTTGGAATCAATTGAGGTAAAAGATAGCTGGCATGCATGCTATTCAGCTCTGTA
TAGACACTATCGATATGTCATTAATAACAGTAAATTTCCAAACTTGTTCATCGATAATTGGTCATGGCATAGATATCAAA
AAGTATTAGATGAAGTTTTAATGTTAAATGCATCAAGGCAAATGATAGGAGAACATGATTTTTTTGCTTTTCAGAAAACT
GGTAGTAATAGAACAAACTCAATTACAAAAATAAAAAATATAGATATAAAAAGAGTAGAAGATTTGATTTTTGTTGATAT
TAAAGCTACTGGTTTTTTATATGGAATGGTTCGCCTAATTATTGGTCAACTAGTTTTAGTTGGAGAAAATAAAATATCGC
CAGAAATTTTTACAGATAGATGGGTAAACAAAAAGAAAAATGATGTTAAAGAATCTGCTCCAGCTAAGGGGTTATGTTTT
GTAAATGCTGTTTATGAAGAAAATGTTTTTAAAAAGATTAATAACAATGATTTTTTTCCTGTATTTATAATTAAGGGTTT
TTCTTAA

Upstream 100 bases:

>100_bases
TTGTTAGAACTGTATCTAGAAAAGGTGATAACGCTCAGATGGCTATAATCGAGCTTGTTTAAGTTAGTTAATTAAATGGG
CTTTTACTAAAAAATAACTT

Downstream 100 bases:

>100_bases
GTAAGGTTTAATTAAGCGAATTTTTTGTGTAAATCATTCAAAACTGTTGTTTAATATTCCTTCAGTAAGGTAGAATTTAA
GACTAATTTAAATTTTATTC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MKRVALLVQYDGSHYSGWQKQKNATTVQEILDRALLKITNHTVQTFAAGRTDAGVHASGQVVHCDVDCVVPGNSYSDVLN
SLLPSSIRILESIEVKDSWHACYSALYRHYRYVINNSKFPNLFIDNWSWHRYQKVLDEVLMLNASRQMIGEHDFFAFQKT
GSNRTNSITKIKNIDIKRVEDLIFVDIKATGFLYGMVRLIIGQLVLVGENKISPEIFTDRWVNKKKNDVKESAPAKGLCF
VNAVYEENVFKKINNNDFFPVFIIKGFS

Sequences:

>Translated_268_residues
MKRVALLVQYDGSHYSGWQKQKNATTVQEILDRALLKITNHTVQTFAAGRTDAGVHASGQVVHCDVDCVVPGNSYSDVLN
SLLPSSIRILESIEVKDSWHACYSALYRHYRYVINNSKFPNLFIDNWSWHRYQKVLDEVLMLNASRQMIGEHDFFAFQKT
GSNRTNSITKIKNIDIKRVEDLIFVDIKATGFLYGMVRLIIGQLVLVGENKISPEIFTDRWVNKKKNDVKESAPAKGLCF
VNAVYEENVFKKINNNDFFPVFIIKGFS
>Mature_268_residues
MKRVALLVQYDGSHYSGWQKQKNATTVQEILDRALLKITNHTVQTFAAGRTDAGVHASGQVVHCDVDCVVPGNSYSDVLN
SLLPSSIRILESIEVKDSWHACYSALYRHYRYVINNSKFPNLFIDNWSWHRYQKVLDEVLMLNASRQMIGEHDFFAFQKT
GSNRTNSITKIKNIDIKRVEDLIFVDIKATGFLYGMVRLIIGQLVLVGENKISPEIFTDRWVNKKKNDVKESAPAKGLCF
VNAVYEENVFKKINNNDFFPVFIIKGFS

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=270, Percent_Identity=26.6666666666667, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI199559785, Length=267, Percent_Identity=29.5880149812734, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI1788657, Length=245, Percent_Identity=33.8775510204082, Blast_Score=148, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6321112, Length=239, Percent_Identity=29.2887029288703, Blast_Score=69, Evalue=6e-13,
Organism=Drosophila melanogaster, GI116007752, Length=307, Percent_Identity=28.3387622149837, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_PROM0 (A3PF23)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001091949.1
- ProteinModelPortal:   A3PF23
- SMR:   A3PF23
- STRING:   A3PF23
- GeneID:   4912386
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_17251
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- BioCyc:   PMAR167546:P9301ORF_1758-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 30645; Mature: 30645

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVALLVQYDGSHYSGWQKQKNATTVQEILDRALLKITNHTVQTFAAGRTDAGVHASGQ
CCCEEEEEEECCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
VVHCDVDCVVPGNSYSDVLNSLLPSSIRILESIEVKDSWHACYSALYRHYRYVINNSKFP
EEEEEEEEEECCCCHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCC
NLFIDNWSWHRYQKVLDEVLMLNASRQMIGEHDFFAFQKTGSNRTNSITKIKNIDIKRVE
EEEEECCCHHHHHHHHHHHHHHCCCHHHHCCCCCEEEECCCCCCCCCCCEEECCCHHHCC
DLIFVDIKATGFLYGMVRLIIGQLVLVGENKISPEIFTDRWVNKKKNDVKESAPAKGLCF
EEEEEEEEHHHHHHHHHHHHHHCEEEECCCCCCCHHHHHHHCCCCHHHHHHCCCCCCEEE
VNAVYEENVFKKINNNDFFPVFIIKGFS
EEHHHHHHHHHHCCCCCCEEEEEEECCC
>Mature Secondary Structure
MKRVALLVQYDGSHYSGWQKQKNATTVQEILDRALLKITNHTVQTFAAGRTDAGVHASGQ
CCCEEEEEEECCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
VVHCDVDCVVPGNSYSDVLNSLLPSSIRILESIEVKDSWHACYSALYRHYRYVINNSKFP
EEEEEEEEEECCCCHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCC
NLFIDNWSWHRYQKVLDEVLMLNASRQMIGEHDFFAFQKTGSNRTNSITKIKNIDIKRVE
EEEEECCCHHHHHHHHHHHHHHCCCHHHHCCCCCEEEECCCCCCCCCCCEEECCCHHHCC
DLIFVDIKATGFLYGMVRLIIGQLVLVGENKISPEIFTDRWVNKKKNDVKESAPAKGLCF
EEEEEEEEHHHHHHHHHHHHHHCEEEECCCCCCCHHHHHHHCCCCHHHHHHCCCCCCEEE
VNAVYEENVFKKINNNDFFPVFIIKGFS
EEHHHHHHHHHHCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA