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The map label for this gene is prfA

Identifier: 126697059

GI number: 126697059

Start: 1456045

End: 1457139

Strand: Reverse

Name: prfA

Synonym: P9301_17211

Alternate gene names: 126697059

Gene position: 1457139-1456045 (Counterclockwise)

Preceding gene: 126697060

Following gene: 126697057

Centisome position: 88.75

GC content: 35.98

Gene sequence:

>1095_bases
TTGGAATACTCAACATTAATTGCAAGGTTGAAAACTGCTTCAGAAAGTTTTGAAAATTTGGAAGTGCAACTTGCAGATCC
TGATATAGCTAATGATCCAAAAAAATTAGAATCAATAGCAAGAGAAAGATCAAAATTGGAACCTTTGGTGCTTGATTTTA
ATAAGTTGCTTGATACTGATAAAGAAATCGAAGATTCAAAAAATCTACTAAAAGAGAATAGAAATGATAAAGAAATGGAA
TCTTTGATAAATGAGGAGTTAATAACACTAGAGGAATCTAAGAGTGAACTGATTCAAAAAACTACAATCGCCTTATTGCC
AAAAGACCCAAGAGATGAAAGAAGTGTAATGTTAGAAATCAGAGCCGGTGCTGGAGGTAACGAGGCTTGTATTTGGGCTG
GTGATTTAGCAAGAATGTATGAAAGATATGGACAAAAAATTGGATGGTCTGTAAAACCTGTTAGTGCTTCCGAGTCAGAT
ATGGGTGGATTTAAGGAGTTAGTTATCTCTGTTAAGGGAGATTCTGTATATAGTCAACTTAAATTTGAGGCTGGTGTACA
TAGAGTCCAAAGAGTTCCAGCTACTGAATCTCAAGGTAGAGTCCACACCTCTACTGCTACAGTTGCCGTAATGCCTGAGG
CTGATCCTGTTGAGGTTAAAATTGATCCAACAGATCTAGAGGTTGGAACAGCAAGATCAGGAGGTGCAGGTGGACAAAAT
GTTAATAAGGTAGAGACAGCTATTGATCTTCTCCACAAGCCTACAGGAATAAGAGTTTTTTGTACTCAAGAGAGATCACA
ATTGCAAAATCGCGAACGAGCAATGGAAATTTTAAGAGCTAAATTGTATGAAATTCAATTAAAAGAAGCCAATGCAAAAG
AGAGATCACAAAGGCTTTCCCAGGTTGGAACAGGCGATAGAAGTGAAAAAATCAGAACTTATAATTTTAAAGATAACAGG
ACAACTGATCACAGATTAGGTTCCAATTTTTCTCTTGAGCCAATTCTTGCTGGTCAATTAGATGAAGTGATTAATGCATG
CATAGCGCAAGAACAAAAAAGAATGATGGAAGATTTTAATAAAGGAGTAAATTAA

Upstream 100 bases:

>100_bases
AAAATATGGAATGGGTTCAGCTAATTCATCCGAATCAAAAGATCAAAAAGAAGAAAAAGATTCTAAAAAATAGAATTTTC
AAAATTAAGCACAATTTCAA

Downstream 100 bases:

>100_bases
GATTTTTTATTAGATTGCAACCCCTATTACGTATTTACTCCATTCTTTATGCTTGCCAGAGTCAATTTGTCTTGTAGCTT
CAAAATTTAGATTACTTAAT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MEYSTLIARLKTASESFENLEVQLADPDIANDPKKLESIARERSKLEPLVLDFNKLLDTDKEIEDSKNLLKENRNDKEME
SLINEELITLEESKSELIQKTTIALLPKDPRDERSVMLEIRAGAGGNEACIWAGDLARMYERYGQKIGWSVKPVSASESD
MGGFKELVISVKGDSVYSQLKFEAGVHRVQRVPATESQGRVHTSTATVAVMPEADPVEVKIDPTDLEVGTARSGGAGGQN
VNKVETAIDLLHKPTGIRVFCTQERSQLQNRERAMEILRAKLYEIQLKEANAKERSQRLSQVGTGDRSEKIRTYNFKDNR
TTDHRLGSNFSLEPILAGQLDEVINACIAQEQKRMMEDFNKGVN

Sequences:

>Translated_364_residues
MEYSTLIARLKTASESFENLEVQLADPDIANDPKKLESIARERSKLEPLVLDFNKLLDTDKEIEDSKNLLKENRNDKEME
SLINEELITLEESKSELIQKTTIALLPKDPRDERSVMLEIRAGAGGNEACIWAGDLARMYERYGQKIGWSVKPVSASESD
MGGFKELVISVKGDSVYSQLKFEAGVHRVQRVPATESQGRVHTSTATVAVMPEADPVEVKIDPTDLEVGTARSGGAGGQN
VNKVETAIDLLHKPTGIRVFCTQERSQLQNRERAMEILRAKLYEIQLKEANAKERSQRLSQVGTGDRSEKIRTYNFKDNR
TTDHRLGSNFSLEPILAGQLDEVINACIAQEQKRMMEDFNKGVN
>Mature_364_residues
MEYSTLIARLKTASESFENLEVQLADPDIANDPKKLESIARERSKLEPLVLDFNKLLDTDKEIEDSKNLLKENRNDKEME
SLINEELITLEESKSELIQKTTIALLPKDPRDERSVMLEIRAGAGGNEACIWAGDLARMYERYGQKIGWSVKPVSASESD
MGGFKELVISVKGDSVYSQLKFEAGVHRVQRVPATESQGRVHTSTATVAVMPEADPVEVKIDPTDLEVGTARSGGAGGQN
VNKVETAIDLLHKPTGIRVFCTQERSQLQNRERAMEILRAKLYEIQLKEANAKERSQRLSQVGTGDRSEKIRTYNFKDNR
TTDHRLGSNFSLEPILAGQLDEVINACIAQEQKRMMEDFNKGVN

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=304, Percent_Identity=40.4605263157895, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI34577120, Length=353, Percent_Identity=34.8441926345609, Blast_Score=222, Evalue=5e-58,
Organism=Homo sapiens, GI166795305, Length=209, Percent_Identity=36.8421052631579, Blast_Score=158, Evalue=9e-39,
Organism=Escherichia coli, GI1787462, Length=350, Percent_Identity=42.5714285714286, Blast_Score=283, Evalue=9e-78,
Organism=Escherichia coli, GI2367172, Length=338, Percent_Identity=34.3195266272189, Blast_Score=198, Evalue=5e-52,
Organism=Caenorhabditis elegans, GI17542784, Length=307, Percent_Identity=36.1563517915309, Blast_Score=186, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6321295, Length=267, Percent_Identity=43.0711610486891, Blast_Score=194, Evalue=2e-50,
Organism=Drosophila melanogaster, GI19921226, Length=302, Percent_Identity=38.4105960264901, Blast_Score=209, Evalue=3e-54,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_PROM0 (A3PF19)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001091945.1
- ProteinModelPortal:   A3PF19
- SMR:   A3PF19
- STRING:   A3PF19
- GeneID:   4912408
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_17211
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- ProtClustDB:   PRK00591
- BioCyc:   PMAR167546:P9301ORF_1754-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40781; Mature: 40781

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYSTLIARLKTASESFENLEVQLADPDIANDPKKLESIARERSKLEPLVLDFNKLLDTD
CCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHCCCHHCCHHHHHCCC
KEIEDSKNLLKENRNDKEMESLINEELITLEESKSELIQKTTIALLPKDPRDERSVMLEI
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEEEEE
RAGAGGNEACIWAGDLARMYERYGQKIGWSVKPVSASESDMGGFKELVISVKGDSVYSQL
ECCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHEEEECCCHHHHHH
KFEAGVHRVQRVPATESQGRVHTSTATVAVMPEADPVEVKIDPTDLEVGTARSGGAGGQN
HHHHHHHHHHHCCCCCCCCEEEECCEEEEEECCCCCEEEEECCCCCEECCCCCCCCCCCC
VNKVETAIDLLHKPTGIRVFCTQERSQLQNRERAMEILRAKLYEIQLKEANAKERSQRLS
HHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
QVGTGDRSEKIRTYNFKDNRTTDHRLGSNFSLEPILAGQLDEVINACIAQEQKRMMEDFN
HHCCCCCCCCEEEECCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KGVN
CCCC
>Mature Secondary Structure
MEYSTLIARLKTASESFENLEVQLADPDIANDPKKLESIARERSKLEPLVLDFNKLLDTD
CCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHCCCHHCCHHHHHCCC
KEIEDSKNLLKENRNDKEMESLINEELITLEESKSELIQKTTIALLPKDPRDERSVMLEI
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEEEEE
RAGAGGNEACIWAGDLARMYERYGQKIGWSVKPVSASESDMGGFKELVISVKGDSVYSQL
ECCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHEEEECCCHHHHHH
KFEAGVHRVQRVPATESQGRVHTSTATVAVMPEADPVEVKIDPTDLEVGTARSGGAGGQN
HHHHHHHHHHHCCCCCCCCEEEECCEEEEEECCCCCEEEEECCCCCEECCCCCCCCCCCC
VNKVETAIDLLHKPTGIRVFCTQERSQLQNRERAMEILRAKLYEIQLKEANAKERSQRLS
HHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
QVGTGDRSEKIRTYNFKDNRTTDHRLGSNFSLEPILAGQLDEVINACIAQEQKRMMEDFN
HHCCCCCCCCEEEECCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KGVN
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA