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The map label for this gene is nadE [H]

Identifier: 126696974

GI number: 126696974

Start: 1368730

End: 1370427

Strand: Direct

Name: nadE [H]

Synonym: P9301_16361

Alternate gene names: 126696974

Gene position: 1368730-1370427 (Clockwise)

Preceding gene: 126696973

Following gene: 126696986

Centisome position: 83.36

GC content: 31.33

Gene sequence:

>1698_bases
ATGAAATTTTTACTTGCCCAAATAAATCCAGTTGTTGGAGATTTAGAGGGCAATGCACAAAAAATACTTAATATTGCTTC
GAAAGCTAGCTCAATTTCTGCTGATTTTGTTCTTACTCCAGAATTGTCATTATGGGGATATCCTGCAAATGACTTACTTT
TTAAAAAAAATTTAATCAAAAATCAATCCCAAATTCTTGATCAATTAGCTTTAGATATTAATAAAAAATATGGAAACTTA
AGCATCACAGTCGGAATAGCTGAGAAGGTAAATGATTCGTTTTTTCCTAATCTGTATAATTCTATTGCGTTGGTTGAGGG
CGGTGAATGGAAAATAATTGCTCGGAAAATTATTCTTCCCACTTATGAAGTATTTGATGAGAAAAGATACTTTAGATCAG
AAGAAAAAGTTTCCGTATTGATTAAACAAATTAAAAATAAAACTTGGCGACTTGGCTTTACTATATGTGAAGATTTATGG
GTCAATAAAGATATAGAAGGTAGAGGAATCCATAGAAAAAATCCCATTATTGATTTAAAGAAAAAAAAAGTTGATATTTT
AGTAAATTTATCAGCCTCCCCATATACTCTGAAAAAATTAGAGCTGAGATCCAAGGTTTCCAGTTTTGCTGCACAATATC
TTCAAGTGCCGCTGATTTATGTTAATCAGATTGGAGCAAATGATAATTTAATTTTTGATGGAAATAGTTTTATTCTTGAT
AAGAATGGATCAAAAATTAAGCAATTAAAATCTTTTTCGGAAGATCTCTCCAGCTGGGAAATTGAGCAAACAAAACCTGA
AAAAAATGAATTTAAAAACTCCGAAATGTCATCAATTTTTGATGCATTAGTCCTTGGTGTTAAAGATTATGCAAAAAAAT
GTGGATTTAAAACAGCTTTAGTTGGATTAAGTGGTGGTATTGATTCAGCACTTGTCTCAGCAATTGCTACAGCAGCTCTA
GGAAGTGATAATGTTTATTGCGTTTCAATGCCCTCTAAGTGGAGCTCATCTCATTCAAAGAGTGATGCAAAAGATTTAGC
AAGAAGATTAAAGATAAGTTTCAAGAGCATTCCAATTGAAAACTTGATGAGCTCTTTTGAGGAATCATTTATAAAAACCA
TATCTTTCGAGATGGCTGAAATAACAAATCAAAATATTCAGTCAAGGATAAGAGGCACGCTTCTTATGGCTTTAGCAAAT
CAAGAAAAGCATTTGTTACTGTCAACAGGAAACAAATCAGAACTAGCCGTAGGATATTGCACACTTTATGGTGATATGAA
TGGGGGATTATCTGTAATTGGTGATTTATATAAAACTAATGTTTTTAAATTATGCAATTGGCTTGATAGTGAAGATTCAA
TTAATAGTAGAAAGTTATATATGTTAAATACTAGTGTAGATATAATTGGAGAAAACATACGTACGAAAGCTCCAAGTGCC
GAATTAGGTCCTGATCAACTAGATACTGACTCTCTACCACCTTACTCTATTTTAGATAAAATTCTTAAAGGAATTATTGA
AGAGAAAAAAGATTTACAACAACTAGAAGAAGATGGTTATAAAAAAGATTTAATTTTAAAAATTATCTCACTCATAAAAA
AAGCGGAATTTAAAAGAAAGCAGGCTCCTCCAATCCTAAAACTAAGCAGTCAATCATTAGGAAGTGACTGGAGAGTTCCC
ATAGCAATATCTTATTAA

Upstream 100 bases:

>100_bases
GTTCTAAGTTTAGGTTAAATTTTAATTGTTCTAATTTACCGTCGTCTTTAATAGATATAGTTAAGAATAATAATTTATAT
GAATCCATCAAATTAGTTGA

Downstream 100 bases:

>100_bases
TCACTTTAAGAACACCGTTGGATTTTTTTTAAAGGCCGTTTAACTGAATCAAGGTTGATACCTTTTTTGATAGCAATAAT
TATGCCTGCAGCTTCTAAAT

Product: carbon-nitrogen hydrolase:NAD+ synthase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 565; Mature: 565

Protein sequence:

>565_residues
MKFLLAQINPVVGDLEGNAQKILNIASKASSISADFVLTPELSLWGYPANDLLFKKNLIKNQSQILDQLALDINKKYGNL
SITVGIAEKVNDSFFPNLYNSIALVEGGEWKIIARKIILPTYEVFDEKRYFRSEEKVSVLIKQIKNKTWRLGFTICEDLW
VNKDIEGRGIHRKNPIIDLKKKKVDILVNLSASPYTLKKLELRSKVSSFAAQYLQVPLIYVNQIGANDNLIFDGNSFILD
KNGSKIKQLKSFSEDLSSWEIEQTKPEKNEFKNSEMSSIFDALVLGVKDYAKKCGFKTALVGLSGGIDSALVSAIATAAL
GSDNVYCVSMPSKWSSSHSKSDAKDLARRLKISFKSIPIENLMSSFEESFIKTISFEMAEITNQNIQSRIRGTLLMALAN
QEKHLLLSTGNKSELAVGYCTLYGDMNGGLSVIGDLYKTNVFKLCNWLDSEDSINSRKLYMLNTSVDIIGENIRTKAPSA
ELGPDQLDTDSLPPYSILDKILKGIIEEKKDLQQLEEDGYKKDLILKIISLIKKAEFKRKQAPPILKLSSQSLGSDWRVP
IAISY

Sequences:

>Translated_565_residues
MKFLLAQINPVVGDLEGNAQKILNIASKASSISADFVLTPELSLWGYPANDLLFKKNLIKNQSQILDQLALDINKKYGNL
SITVGIAEKVNDSFFPNLYNSIALVEGGEWKIIARKIILPTYEVFDEKRYFRSEEKVSVLIKQIKNKTWRLGFTICEDLW
VNKDIEGRGIHRKNPIIDLKKKKVDILVNLSASPYTLKKLELRSKVSSFAAQYLQVPLIYVNQIGANDNLIFDGNSFILD
KNGSKIKQLKSFSEDLSSWEIEQTKPEKNEFKNSEMSSIFDALVLGVKDYAKKCGFKTALVGLSGGIDSALVSAIATAAL
GSDNVYCVSMPSKWSSSHSKSDAKDLARRLKISFKSIPIENLMSSFEESFIKTISFEMAEITNQNIQSRIRGTLLMALAN
QEKHLLLSTGNKSELAVGYCTLYGDMNGGLSVIGDLYKTNVFKLCNWLDSEDSINSRKLYMLNTSVDIIGENIRTKAPSA
ELGPDQLDTDSLPPYSILDKILKGIIEEKKDLQQLEEDGYKKDLILKIISLIKKAEFKRKQAPPILKLSSQSLGSDWRVP
IAISY
>Mature_565_residues
MKFLLAQINPVVGDLEGNAQKILNIASKASSISADFVLTPELSLWGYPANDLLFKKNLIKNQSQILDQLALDINKKYGNL
SITVGIAEKVNDSFFPNLYNSIALVEGGEWKIIARKIILPTYEVFDEKRYFRSEEKVSVLIKQIKNKTWRLGFTICEDLW
VNKDIEGRGIHRKNPIIDLKKKKVDILVNLSASPYTLKKLELRSKVSSFAAQYLQVPLIYVNQIGANDNLIFDGNSFILD
KNGSKIKQLKSFSEDLSSWEIEQTKPEKNEFKNSEMSSIFDALVLGVKDYAKKCGFKTALVGLSGGIDSALVSAIATAAL
GSDNVYCVSMPSKWSSSHSKSDAKDLARRLKISFKSIPIENLMSSFEESFIKTISFEMAEITNQNIQSRIRGTLLMALAN
QEKHLLLSTGNKSELAVGYCTLYGDMNGGLSVIGDLYKTNVFKLCNWLDSEDSINSRKLYMLNTSVDIIGENIRTKAPSA
ELGPDQLDTDSLPPYSILDKILKGIIEEKKDLQQLEEDGYKKDLILKIISLIKKAEFKRKQAPPILKLSSQSLGSDWRVP
IAISY

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 63187; Mature: 63187

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFLLAQINPVVGDLEGNAQKILNIASKASSISADFVLTPELSLWGYPANDLLFKKNLIK
CCEEEECCCCHHCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHHHHHHHHC
NQSQILDQLALDINKKYGNLSITVGIAEKVNDSFFPNLYNSIALVEGGEWKIIARKIILP
CHHHHHHHHHHHHCCCCCCEEEEEEEHHHCCCCCCCHHHCCEEEEECCCEEEEEEEHHCC
TYEVFDEKRYFRSEEKVSVLIKQIKNKTWRLGFTICEDLWVNKDIEGRGIHRKNPIIDLK
CHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCCCCCCCCCCEEEC
KKKVDILVNLSASPYTLKKLELRSKVSSFAAQYLQVPLIYVNQIGANDNLIFDGNSFILD
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEECCCEEEEE
KNGSKIKQLKSFSEDLSSWEIEQTKPEKNEFKNSEMSSIFDALVLGVKDYAKKCGFKTAL
CCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
VGLSGGIDSALVSAIATAALGSDNVYCVSMPSKWSSSHSKSDAKDLARRLKISFKSIPIE
EECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCHH
NLMSSFEESFIKTISFEMAEITNQNIQSRIRGTLLMALANQEKHLLLSTGNKSELAVGYC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCCEEEEEE
TLYGDMNGGLSVIGDLYKTNVFKLCNWLDSEDSINSRKLYMLNTSVDIIGENIRTKAPSA
EEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHCCCCCCCCCCC
ELGPDQLDTDSLPPYSILDKILKGIIEEKKDLQQLEEDGYKKDLILKIISLIKKAEFKRK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
QAPPILKLSSQSLGSDWRVPIAISY
CCCCEEEECCCCCCCCCCEEEEEEC
>Mature Secondary Structure
MKFLLAQINPVVGDLEGNAQKILNIASKASSISADFVLTPELSLWGYPANDLLFKKNLIK
CCEEEECCCCHHCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHHHHHHHHC
NQSQILDQLALDINKKYGNLSITVGIAEKVNDSFFPNLYNSIALVEGGEWKIIARKIILP
CHHHHHHHHHHHHCCCCCCEEEEEEEHHHCCCCCCCHHHCCEEEEECCCEEEEEEEHHCC
TYEVFDEKRYFRSEEKVSVLIKQIKNKTWRLGFTICEDLWVNKDIEGRGIHRKNPIIDLK
CHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCCCCCCCCCCEEEC
KKKVDILVNLSASPYTLKKLELRSKVSSFAAQYLQVPLIYVNQIGANDNLIFDGNSFILD
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEECCCEEEEE
KNGSKIKQLKSFSEDLSSWEIEQTKPEKNEFKNSEMSSIFDALVLGVKDYAKKCGFKTAL
CCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
VGLSGGIDSALVSAIATAALGSDNVYCVSMPSKWSSSHSKSDAKDLARRLKISFKSIPIE
EECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCHH
NLMSSFEESFIKTISFEMAEITNQNIQSRIRGTLLMALANQEKHLLLSTGNKSELAVGYC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCCEEEEEE
TLYGDMNGGLSVIGDLYKTNVFKLCNWLDSEDSINSRKLYMLNTSVDIIGENIRTKAPSA
EEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHCCCCCCCCCCC
ELGPDQLDTDSLPPYSILDKILKGIIEEKKDLQQLEEDGYKKDLILKIISLIKKAEFKRK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
QAPPILKLSSQSLGSDWRVPIAISY
CCCCEEEECCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]