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The map label for this gene is pnp

Identifier: 126696730

GI number: 126696730

Start: 1159240

End: 1161405

Strand: Direct

Name: pnp

Synonym: P9301_13921

Alternate gene names: 126696730

Gene position: 1159240-1161405 (Clockwise)

Preceding gene: 126696729

Following gene: 126696732

Centisome position: 70.6

GC content: 38.37

Gene sequence:

>2166_bases
GTGGAAGGACAAAATAAGTCGATCACGTTTGACGGACGAGAGATACGACTAACAACAGGACTATATGCTCCTCAAGCAAA
TGGATCAGTAATGATTGAATGTGGAGACACATCTTTATTAGTTACAGCAACAAAAACTACAAAAAAAGAAGTTTCAGACT
TTCTCCCTCTAATATGCGATTATGAGGAAAAATTATATGCTGCGGGGAGAATTCCAGGCGGTTTCATGAGAAGAGAGGGC
CGCCCTCCAGAAAGAGCAACTTTAATTTCAAGATTGATTGATAGGCCTATGAGACCACTTTTCCCCTCTTGGATGAGAGA
CGAGATACAGATAGTGGCTTCCTGCCTTTCTCTTGATGAGAGAGTACCTGCAGACATTCTCGCTGTTACAGGTGCTTCAA
TAGCAACCTTGCTCGGAGAGATACCATTTTACGGGCCAATGGCTGCAGTAAGAGTTGGGCTAATAGGAGACGACTTTATA
CTAAATCCAAGCTATAGAGAGATAGAAAAAGGAGATTTAGATATTGTTGTTGCAGGCTCAAAAGAAGGAATTGTGATGAT
TGAGGCAGGTGCTAACCAATTATCAGAACAAGATACTATAGAGGCAATAGATTTTGGATATGAGGCTGTATCAGAACTTA
TTAAATCTCAAGAAGATTTACTAAAAGATTTAGGAATAAAGCAGGTTAAGCCATCCGAACCTGAAGAAGATAAAACATTG
CCTTCTTTTTTAGAGAAAAATTGTACAAAACCTATTGAGTTAGTTTTAAAGAAATTTGATCTTTCAAAAGAGGAGAGAGA
TCTTGAACTCGAAAAAATAAAAGTTGAGACTCAAGGTAAAATTGAATCCTTGAAAGATGATAATCAACTAAAAGTTCTTC
TTTCGGAGAATGATAAGTTATTAAGTTCCGACTTTAAAAAACTTACAAAGAAATTAATGAGGTCGCAAATTATTAATGAT
GGTAAGAGAGTTGATGGAAGAGATCTAGATGAAGTTAGAAAAATTTCAGCTTCTGCAGGTATTCTTCCAAAAAGAGTTCA
TGGTTCTGCATTATTTCAAAGAGGTTTGACTCAAGTTTTATCAACAACCACATTAGGGACTCCTAGTGATGCTCAAGAAA
TGGACGACCTGAATCCAAGCACTGAAAAAACTTATCTACATCACTATAACTTTCCTCCTTATTCAGTAGGAGAAACAAGA
CCAATGAGGACACCTGGCAGAAGAGAAATAGGTCATGGAGCATTAGCTGAGAGGGCAATAATTCCTGTATTGCCTGGCAA
AGAGACATTTCCATACGTGCTTAGGGTAGTAAGTGAGGTCTTAAGCTCTAATGGATCAACCTCAATGGGATCAGTATGCG
GCAGCACATTATCACTATTAGATGCTGGAGTACCACTAAAGGCGCCAGTTAGCGGAACTGCAATGGGTTTAATTAAAGAA
GGAAAGGAAGTTAGAATTCTTACCGATATCCAGGGAATTGAAGATTTTCTAGGAGACATGGACTTTAAAGTTGCAGGTAC
TGATAAAGGAATAACTGCTTTACAAATGGATATGAAAATTACAGGTTTACCAGTTTCTATTATTTCTGATGCAATTAAAA
AAGCTCGGCCTGCAAGATTACATATTTTAGAAAAGATGCAAGAAGCTATTGAAAAACCACAAGAAACGTTATCTCCTCAT
GCACCAAGGCTGTTAAGTTTTAGAATTGACCCAGAACTTATTGGAACTGTAATTGGCCCTGGTGGAAGAACTATTAAAGG
GATAACAGAAAGAACAAACACAAAAATAGATATTGAAGATGGAGGAATTGTTACTATTGCGTCGCATGATGGAGCTGCCG
CAGAGGAAGCACAGAAGATTATAGAGGGATTAACTAGAAAGGTTCATGAAGGAGAAATTTTCCCTGGGGTCGTAACCCGA
ATTATTCCAATAGGAGCATTTGTAGAAATACTACCCGGCAAGGAAGGTATGGTTCATATATCCCAATTATCTGAAGCCAG
AGTTGAGAGGGTTGAAGATGTTGTTAGGCAAGGAGATGAAGTAACTGTTAGAGTTAGAGAAATTGATAGCAGAGGTAGGA
TAAATCTTACTTTAAGAGGAGTTGGTCAAAATGGTGGCATGTCTTATCCCGAACCCACACCTACTCCAGTAGCCCCCCTT
AGTTAA

Upstream 100 bases:

>100_bases
CAGAACAATTTTAAAAATTTTACAGCTTATTTAAATAATTGACTCAATATGTCCCTATAAAATGTAAGAATAAATTATGT
ATAGATTTATAATTTAAAAA

Downstream 100 bases:

>100_bases
GTCAAAGAGGAAATAAATCACCTTCTCTGATTATTTCCTCTATCTCAGAACAAATGTTTTTATGATTATTAATATTATTA
GATGCCACGATTATCCCACC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 721; Mature: 721

Protein sequence:

>721_residues
MEGQNKSITFDGREIRLTTGLYAPQANGSVMIECGDTSLLVTATKTTKKEVSDFLPLICDYEEKLYAAGRIPGGFMRREG
RPPERATLISRLIDRPMRPLFPSWMRDEIQIVASCLSLDERVPADILAVTGASIATLLGEIPFYGPMAAVRVGLIGDDFI
LNPSYREIEKGDLDIVVAGSKEGIVMIEAGANQLSEQDTIEAIDFGYEAVSELIKSQEDLLKDLGIKQVKPSEPEEDKTL
PSFLEKNCTKPIELVLKKFDLSKEERDLELEKIKVETQGKIESLKDDNQLKVLLSENDKLLSSDFKKLTKKLMRSQIIND
GKRVDGRDLDEVRKISASAGILPKRVHGSALFQRGLTQVLSTTTLGTPSDAQEMDDLNPSTEKTYLHHYNFPPYSVGETR
PMRTPGRREIGHGALAERAIIPVLPGKETFPYVLRVVSEVLSSNGSTSMGSVCGSTLSLLDAGVPLKAPVSGTAMGLIKE
GKEVRILTDIQGIEDFLGDMDFKVAGTDKGITALQMDMKITGLPVSIISDAIKKARPARLHILEKMQEAIEKPQETLSPH
APRLLSFRIDPELIGTVIGPGGRTIKGITERTNTKIDIEDGGIVTIASHDGAAAEEAQKIIEGLTRKVHEGEIFPGVVTR
IIPIGAFVEILPGKEGMVHISQLSEARVERVEDVVRQGDEVTVRVREIDSRGRINLTLRGVGQNGGMSYPEPTPTPVAPL
S

Sequences:

>Translated_721_residues
MEGQNKSITFDGREIRLTTGLYAPQANGSVMIECGDTSLLVTATKTTKKEVSDFLPLICDYEEKLYAAGRIPGGFMRREG
RPPERATLISRLIDRPMRPLFPSWMRDEIQIVASCLSLDERVPADILAVTGASIATLLGEIPFYGPMAAVRVGLIGDDFI
LNPSYREIEKGDLDIVVAGSKEGIVMIEAGANQLSEQDTIEAIDFGYEAVSELIKSQEDLLKDLGIKQVKPSEPEEDKTL
PSFLEKNCTKPIELVLKKFDLSKEERDLELEKIKVETQGKIESLKDDNQLKVLLSENDKLLSSDFKKLTKKLMRSQIIND
GKRVDGRDLDEVRKISASAGILPKRVHGSALFQRGLTQVLSTTTLGTPSDAQEMDDLNPSTEKTYLHHYNFPPYSVGETR
PMRTPGRREIGHGALAERAIIPVLPGKETFPYVLRVVSEVLSSNGSTSMGSVCGSTLSLLDAGVPLKAPVSGTAMGLIKE
GKEVRILTDIQGIEDFLGDMDFKVAGTDKGITALQMDMKITGLPVSIISDAIKKARPARLHILEKMQEAIEKPQETLSPH
APRLLSFRIDPELIGTVIGPGGRTIKGITERTNTKIDIEDGGIVTIASHDGAAAEEAQKIIEGLTRKVHEGEIFPGVVTR
IIPIGAFVEILPGKEGMVHISQLSEARVERVEDVVRQGDEVTVRVREIDSRGRINLTLRGVGQNGGMSYPEPTPTPVAPL
S
>Mature_721_residues
MEGQNKSITFDGREIRLTTGLYAPQANGSVMIECGDTSLLVTATKTTKKEVSDFLPLICDYEEKLYAAGRIPGGFMRREG
RPPERATLISRLIDRPMRPLFPSWMRDEIQIVASCLSLDERVPADILAVTGASIATLLGEIPFYGPMAAVRVGLIGDDFI
LNPSYREIEKGDLDIVVAGSKEGIVMIEAGANQLSEQDTIEAIDFGYEAVSELIKSQEDLLKDLGIKQVKPSEPEEDKTL
PSFLEKNCTKPIELVLKKFDLSKEERDLELEKIKVETQGKIESLKDDNQLKVLLSENDKLLSSDFKKLTKKLMRSQIIND
GKRVDGRDLDEVRKISASAGILPKRVHGSALFQRGLTQVLSTTTLGTPSDAQEMDDLNPSTEKTYLHHYNFPPYSVGETR
PMRTPGRREIGHGALAERAIIPVLPGKETFPYVLRVVSEVLSSNGSTSMGSVCGSTLSLLDAGVPLKAPVSGTAMGLIKE
GKEVRILTDIQGIEDFLGDMDFKVAGTDKGITALQMDMKITGLPVSIISDAIKKARPARLHILEKMQEAIEKPQETLSPH
APRLLSFRIDPELIGTVIGPGGRTIKGITERTNTKIDIEDGGIVTIASHDGAAAEEAQKIIEGLTRKVHEGEIFPGVVTR
IIPIGAFVEILPGKEGMVHISQLSEARVERVEDVVRQGDEVTVRVREIDSRGRINLTLRGVGQNGGMSYPEPTPTPVAPL
S

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=718, Percent_Identity=35.7938718662953, Blast_Score=437, Evalue=1e-122,
Organism=Escherichia coli, GI145693187, Length=701, Percent_Identity=45.5064194008559, Blast_Score=597, Evalue=1e-171,
Organism=Caenorhabditis elegans, GI115534063, Length=677, Percent_Identity=34.2688330871492, Blast_Score=348, Evalue=4e-96,
Organism=Drosophila melanogaster, GI281362905, Length=681, Percent_Identity=37.2980910425844, Blast_Score=430, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24651641, Length=681, Percent_Identity=37.2980910425844, Blast_Score=430, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24651643, Length=681, Percent_Identity=37.2980910425844, Blast_Score=430, Evalue=1e-120,
Organism=Drosophila melanogaster, GI161079377, Length=625, Percent_Identity=37.28, Blast_Score=395, Evalue=1e-110,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_PROM0 (A3PE40)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001091616.1
- ProteinModelPortal:   A3PE40
- SMR:   A3PE40
- STRING:   A3PE40
- GeneID:   4912214
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_13921
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- BioCyc:   PMAR167546:P9301ORF_1414-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 78782; Mature: 78782

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGQNKSITFDGREIRLTTGLYAPQANGSVMIECGDTSLLVTATKTTKKEVSDFLPLICD
CCCCCCCEEECCCEEEEEECEECCCCCCEEEEEECCCEEEEEECCHHHHHHHHHHHHHCC
YEEKLYAAGRIPGGFMRREGRPPERATLISRLIDRPMRPLFPSWMRDEIQIVASCLSLDE
CHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHC
RVPADILAVTGASIATLLGEIPFYGPMAAVRVGLIGDDFILNPSYREIEKGDLDIVVAGS
CCCHHHHHHCCHHHHHHHHCCCCCCCHHHHEEEEECCCEEECCCHHHHCCCCEEEEEECC
KEGIVMIEAGANQLSEQDTIEAIDFGYEAVSELIKSQEDLLKDLGIKQVKPSEPEEDKTL
CCCEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
PSFLEKNCTKPIELVLKKFDLSKEERDLELEKIKVETQGKIESLKDDNQLKVLLSENDKL
HHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEEEEEECCCCHHHCCCCCCEEEEECCCCCH
LSSDFKKLTKKLMRSQIINDGKRVDGRDLDEVRKISASAGILPKRVHGSALFQRGLTQVL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
STTTLGTPSDAQEMDDLNPSTEKTYLHHYNFPPYSVGETRPMRTPGRREIGHGALAERAI
HHCCCCCCCCHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHCCCCHHHHCCE
IPVLPGKETFPYVLRVVSEVLSSNGSTSMGSVCGSTLSLLDAGVPLKAPVSGTAMGLIKE
EECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHC
GKEVRILTDIQGIEDFLGDMDFKVAGTDKGITALQMDMKITGLPVSIISDAIKKARPARL
CCEEEEEEHHHHHHHHHCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHCCCHHH
HILEKMQEAIEKPQETLSPHAPRLLSFRIDPELIGTVIGPGGRTIKGITERTNTKIDIED
HHHHHHHHHHHCHHHHCCCCCCCEEEEEECHHHHHEEECCCCCEEECHHHCCCCEEEECC
GGIVTIASHDGAAAEEAQKIIEGLTRKVHEGEIFPGVVTRIIPIGAFVEILPGKEGMVHI
CCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEECCCCCCCEEH
SQLSEARVERVEDVVRQGDEVTVRVREIDSRGRINLTLRGVGQNGGMSYPEPTPTPVAPL
HHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCC
S
C
>Mature Secondary Structure
MEGQNKSITFDGREIRLTTGLYAPQANGSVMIECGDTSLLVTATKTTKKEVSDFLPLICD
CCCCCCCEEECCCEEEEEECEECCCCCCEEEEEECCCEEEEEECCHHHHHHHHHHHHHCC
YEEKLYAAGRIPGGFMRREGRPPERATLISRLIDRPMRPLFPSWMRDEIQIVASCLSLDE
CHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHC
RVPADILAVTGASIATLLGEIPFYGPMAAVRVGLIGDDFILNPSYREIEKGDLDIVVAGS
CCCHHHHHHCCHHHHHHHHCCCCCCCHHHHEEEEECCCEEECCCHHHHCCCCEEEEEECC
KEGIVMIEAGANQLSEQDTIEAIDFGYEAVSELIKSQEDLLKDLGIKQVKPSEPEEDKTL
CCCEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
PSFLEKNCTKPIELVLKKFDLSKEERDLELEKIKVETQGKIESLKDDNQLKVLLSENDKL
HHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEEEEEECCCCHHHCCCCCCEEEEECCCCCH
LSSDFKKLTKKLMRSQIINDGKRVDGRDLDEVRKISASAGILPKRVHGSALFQRGLTQVL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
STTTLGTPSDAQEMDDLNPSTEKTYLHHYNFPPYSVGETRPMRTPGRREIGHGALAERAI
HHCCCCCCCCHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHCCCCHHHHCCE
IPVLPGKETFPYVLRVVSEVLSSNGSTSMGSVCGSTLSLLDAGVPLKAPVSGTAMGLIKE
EECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHC
GKEVRILTDIQGIEDFLGDMDFKVAGTDKGITALQMDMKITGLPVSIISDAIKKARPARL
CCEEEEEEHHHHHHHHHCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHCCCHHH
HILEKMQEAIEKPQETLSPHAPRLLSFRIDPELIGTVIGPGGRTIKGITERTNTKIDIED
HHHHHHHHHHHCHHHHCCCCCCCEEEEEECHHHHHEEECCCCCEEECHHHCCCCEEEECC
GGIVTIASHDGAAAEEAQKIIEGLTRKVHEGEIFPGVVTRIIPIGAFVEILPGKEGMVHI
CCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEECCCCCCCEEH
SQLSEARVERVEDVVRQGDEVTVRVREIDSRGRINLTLRGVGQNGGMSYPEPTPTPVAPL
HHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA