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The map label for this gene is leuA

Identifier: 126696510

GI number: 126696510

Start: 984455

End: 986095

Strand: Direct

Name: leuA

Synonym: P9301_11721

Alternate gene names: 126696510

Gene position: 984455-986095 (Clockwise)

Preceding gene: 126696508

Following gene: 126696511

Centisome position: 59.96

GC content: 35.41

Gene sequence:

>1641_bases
ATGTCAAAAAATCCTGGAAGAATTTTGATATTTGATACAACTCTTCGAGATGGAGAGCAATCTCCTGGTGCAAGTTTAAA
TCTTGAAGAAAAACTTGCCATAGCTCATCAATTAGCAAGATTAGGGGTAGATGTTATTGAAGCTGGATTCCCTTTCGCAA
GTCCAGGAGATTTTAAAGCTGTTAATAAAATTGCCAGTGCCGTTGGGAAAGAGCATGGGCCTACAATATGTGGTTTAGCT
AGAGCGTCTAAAGGAGATATAAAAGCATGTTACGAAGCAGTAAGTCCAGCTCCCAAGAAAAGAATACATACTTTTATTGC
TACAAGTGATATTCATCTTAAACATAAACTTAAAAAATCCAGAAAAGATGTTCTTCAAATAGTCCCAGAAATGGTTAATT
ATGCCAAATCATTGGTTGATGATATTGAGTTTTCTTGTGAAGATGCCTCAAGGAGTGATCCTGATTTTTTATACGAAGTG
ATTCAACTAGCAATTTCTGCAGGAGCGACAACAATCAATATCCCCGATACTGTTGGATTTACAACTCCAAGTGAATTTGG
CAACCTAATTGCCGATATAAATAAAAATGTTCCAAATATTGATGAGGCAGTAATCTCGGTTCATGGTCATAATGATCTGG
GTTTAGCAGTAGCCAATTTTCTTGAAGCTGTAAAAAATGGTGCAAGGCAACTAGAGTGTACTATAAATGGAATTGGTGAA
AGAGCCGGGAATGCCTCTTTAGAAGAATTGGTCATGGCTCTACATGTTAGGAAAAGTTTTTTTAATAGTTTTTTCAAAAG
AAATCCAGATTCACCAACTCCTCTTACAGCTATAAGAACAGAAGAAATAACAAAAACCTCAAGACTTGTTTCCAACCTAA
CTGGAATGACTGTACAACCTAATAAAGCAATTGTGGGAGCTAACGCTTTTGCACATGAGTCAGGCATTCATCAGGATGGT
GTTTTAAAAAATAGACTAACTTACGAAATTATCGATGCAAAAACTGTTGGTTTGAGTGACAACAAAATATCTTTGGGGAA
ACTTAGTGGAAGAAGTGCAGTTAGAGCAAGATTAGAAGAAATGGGATATGATTTGAGTCGAGAAGATTTAAATGATGCTT
TTGCCCGTTTTAAGGATTTAGCAGACAGGAAAAGAGAAATTACTGATAGAGACTTAGAAGCAATTGTTAGTGAACAAGTT
CAACTCCCAGAAGCTAAATTTCAATTAAGTCTTGTACAAGTAAGTTGCGGTAACGCATCTAAACCTACTGCCACCATTTC
GCTTTTAAATACAGAAGATAATACTGAGGATACGGCTGTATCAATAGGGACAGGACCCGTTGACGCTGTATGCGAGGCTT
TAAATAAATTAGCTAAAGTTCCTAATGAATTAATTGAATTTTCTGTTAAGTCAGTAACAGAAGGAATTGATGCTTTGGGC
GAAGTGACAATAAGAATAAGAAGGGATAATAAAATATATTCTGGTCATTCTGCTGATACGGATGTTGTTGTTGCTGCAGC
GAATGCTTACATTAATGCCTTAAATAGGCTTGTATTTTCTGAGAAAAAAAATTCAATTCATCCACAATTTGATAATTTAG
AAAATTCTAATAATACATATATATCTAATCCTGCAAATTAA

Upstream 100 bases:

>100_bases
CCAATATTATTTAAGTTATAAATTTGCAGCAAATTTTTTTAAATTAAATCTAGTCAATTTTTTTTAATTAGCTTAATATA
AGGCTAGTATCATTCATCTT

Downstream 100 bases:

>100_bases
ATTATTTCTTAGGATTATTAAGTGTCATTAAAAAATAGTCTCTTAATACTGTAGATTAAATTTATAGTTAAAGCAGCAAA
TAATGAGAGAAAGGTTACTT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MSKNPGRILIFDTTLRDGEQSPGASLNLEEKLAIAHQLARLGVDVIEAGFPFASPGDFKAVNKIASAVGKEHGPTICGLA
RASKGDIKACYEAVSPAPKKRIHTFIATSDIHLKHKLKKSRKDVLQIVPEMVNYAKSLVDDIEFSCEDASRSDPDFLYEV
IQLAISAGATTINIPDTVGFTTPSEFGNLIADINKNVPNIDEAVISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE
RAGNASLEELVMALHVRKSFFNSFFKRNPDSPTPLTAIRTEEITKTSRLVSNLTGMTVQPNKAIVGANAFAHESGIHQDG
VLKNRLTYEIIDAKTVGLSDNKISLGKLSGRSAVRARLEEMGYDLSREDLNDAFARFKDLADRKREITDRDLEAIVSEQV
QLPEAKFQLSLVQVSCGNASKPTATISLLNTEDNTEDTAVSIGTGPVDAVCEALNKLAKVPNELIEFSVKSVTEGIDALG
EVTIRIRRDNKIYSGHSADTDVVVAAANAYINALNRLVFSEKKNSIHPQFDNLENSNNTYISNPAN

Sequences:

>Translated_546_residues
MSKNPGRILIFDTTLRDGEQSPGASLNLEEKLAIAHQLARLGVDVIEAGFPFASPGDFKAVNKIASAVGKEHGPTICGLA
RASKGDIKACYEAVSPAPKKRIHTFIATSDIHLKHKLKKSRKDVLQIVPEMVNYAKSLVDDIEFSCEDASRSDPDFLYEV
IQLAISAGATTINIPDTVGFTTPSEFGNLIADINKNVPNIDEAVISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE
RAGNASLEELVMALHVRKSFFNSFFKRNPDSPTPLTAIRTEEITKTSRLVSNLTGMTVQPNKAIVGANAFAHESGIHQDG
VLKNRLTYEIIDAKTVGLSDNKISLGKLSGRSAVRARLEEMGYDLSREDLNDAFARFKDLADRKREITDRDLEAIVSEQV
QLPEAKFQLSLVQVSCGNASKPTATISLLNTEDNTEDTAVSIGTGPVDAVCEALNKLAKVPNELIEFSVKSVTEGIDALG
EVTIRIRRDNKIYSGHSADTDVVVAAANAYINALNRLVFSEKKNSIHPQFDNLENSNNTYISNPAN
>Mature_545_residues
SKNPGRILIFDTTLRDGEQSPGASLNLEEKLAIAHQLARLGVDVIEAGFPFASPGDFKAVNKIASAVGKEHGPTICGLAR
ASKGDIKACYEAVSPAPKKRIHTFIATSDIHLKHKLKKSRKDVLQIVPEMVNYAKSLVDDIEFSCEDASRSDPDFLYEVI
QLAISAGATTINIPDTVGFTTPSEFGNLIADINKNVPNIDEAVISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGER
AGNASLEELVMALHVRKSFFNSFFKRNPDSPTPLTAIRTEEITKTSRLVSNLTGMTVQPNKAIVGANAFAHESGIHQDGV
LKNRLTYEIIDAKTVGLSDNKISLGKLSGRSAVRARLEEMGYDLSREDLNDAFARFKDLADRKREITDRDLEAIVSEQVQ
LPEAKFQLSLVQVSCGNASKPTATISLLNTEDNTEDTAVSIGTGPVDAVCEALNKLAKVPNELIEFSVKSVTEGIDALGE
VTIRIRRDNKIYSGHSADTDVVVAAANAYINALNRLVFSEKKNSIHPQFDNLENSNNTYISNPAN

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=513, Percent_Identity=46.588693957115, Blast_Score=439, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6320019, Length=387, Percent_Identity=29.4573643410853, Blast_Score=139, Evalue=8e-34,
Organism=Saccharomyces cerevisiae, GI6320071, Length=389, Percent_Identity=28.7917737789203, Blast_Score=137, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6324225, Length=555, Percent_Identity=25.2252252252252, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6324682, Length=507, Percent_Identity=24.65483234714, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_PROM0 (A3PDH0)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001091396.1
- ProteinModelPortal:   A3PDH0
- SMR:   A3PDH0
- STRING:   A3PDH0
- GeneID:   4911955
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_11721
- eggNOG:   COG0119
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- ProtClustDB:   PRK00915
- BioCyc:   PMAR167546:P9301ORF_1193-MONOMER
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 59176; Mature: 59045

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNPGRILIFDTTLRDGEQSPGASLNLEEKLAIAHQLARLGVDVIEAGFPFASPGDFKA
CCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHH
VNKIASAVGKEHGPTICGLARASKGDIKACYEAVSPAPKKRIHTFIATSDIHLKHKLKKS
HHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHEECCHHHHHHHHHH
RKDVLQIVPEMVNYAKSLVDDIEFSCEDASRSDPDFLYEVIQLAISAGATTINIPDTVGF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCC
TTPSEFGNLIADINKNVPNIDEAVISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE
CCHHHHHHHHHHHCCCCCCCCHHEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECHHC
RAGNASLEELVMALHVRKSFFNSFFKRNPDSPTPLTAIRTEEITKTSRLVSNLTGMTVQP
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHCCCEECC
NKAIVGANAFAHESGIHQDGVLKNRLTYEIIDAKTVGLSDNKISLGKLSGRSAVRARLEE
CCEEEECCHHHHCCCCCCCCCHHCCCEEEEECCEEECCCCCCEEECCCCCHHHHHHHHHH
MGYDLSREDLNDAFARFKDLADRKREITDRDLEAIVSEQVQLPEAKFQLSLVQVSCGNAS
HCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHEEEEEEEEECCCCC
KPTATISLLNTEDNTEDTAVSIGTGPVDAVCEALNKLAKVPNELIEFSVKSVTEGIDALG
CCCEEEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHC
EVTIRIRRDNKIYSGHSADTDVVVAAANAYINALNRLVFSEKKNSIHPQFDNLENSNNTY
EEEEEEEECCEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEE
ISNPAN
ECCCCC
>Mature Secondary Structure 
SKNPGRILIFDTTLRDGEQSPGASLNLEEKLAIAHQLARLGVDVIEAGFPFASPGDFKA
CCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHH
VNKIASAVGKEHGPTICGLARASKGDIKACYEAVSPAPKKRIHTFIATSDIHLKHKLKKS
HHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHEECCHHHHHHHHHH
RKDVLQIVPEMVNYAKSLVDDIEFSCEDASRSDPDFLYEVIQLAISAGATTINIPDTVGF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCC
TTPSEFGNLIADINKNVPNIDEAVISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE
CCHHHHHHHHHHHCCCCCCCCHHEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECHHC
RAGNASLEELVMALHVRKSFFNSFFKRNPDSPTPLTAIRTEEITKTSRLVSNLTGMTVQP
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHCCCEECC
NKAIVGANAFAHESGIHQDGVLKNRLTYEIIDAKTVGLSDNKISLGKLSGRSAVRARLEE
CCEEEECCHHHHCCCCCCCCCHHCCCEEEEECCEEECCCCCCEEECCCCCHHHHHHHHHH
MGYDLSREDLNDAFARFKDLADRKREITDRDLEAIVSEQVQLPEAKFQLSLVQVSCGNAS
HCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHEEEEEEEEECCCCC
KPTATISLLNTEDNTEDTAVSIGTGPVDAVCEALNKLAKVPNELIEFSVKSVTEGIDALG
CCCEEEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHC
EVTIRIRRDNKIYSGHSADTDVVVAAANAYINALNRLVFSEKKNSIHPQFDNLENSNNTY
EEEEEEEECCEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEE
ISNPAN
ECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA