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The map label for this gene is metG [H]

Identifier: 126696331

GI number: 126696331

Start: 864960

End: 866504

Strand: Direct

Name: metG [H]

Synonym: P9301_09931

Alternate gene names: 126696331

Gene position: 864960-866504 (Clockwise)

Preceding gene: 126696330

Following gene: 126696332

Centisome position: 52.68

GC content: 29.97

Gene sequence:

>1545_bases
ATGACTTTTGTCATTACTACACCTTTATACTATGTTAATGATAAACCTCATTTAGGGAGTGTATATACGACAATAATTTG
TGACTCAATAGCTAGGTATAAAAGGCTTGCAGGTGAAGATGTTATTTTCATCACTGGTGTTGATGAACATGGTTTAAAAA
TACAGAGAACAGCTAATGAAAAGGGTATTGAACCAAAATCACATTGTGATGAAATTTCAGAAGTTTTTAATAATAATTGG
AAAGATTGGAATATATCCTTTGACAAATTTATAAGAACAAGCTCCAAAAATCATGAATTTGTTGTTAATGAATTTTATGA
AAGAGTAAAAGCATCAGATGATATCTATATGGGAGTTCAAAAAGGTTGGTATTGTGTCGGTTGTGAAGAATTTAAAGATA
ATCCAGAAAACTCATCAACATACAAGTGTCCAATACATCAAAAAAATCTAGAATGGAAAAATGAAGAGAATCTCTTTTTT
AGGCTTTCAAAATATCAAAAAGAAATCGAGAGAATAATCAACGAACCTTCTTTTATAGAGCCAATAGAAAGAAAGAATGA
AATTATAAATTTTGTTTCTAGAGGTTTAAAGGATTTTTCAATTTCAAGAACAAATGTTAAATGGGGAATTCCTGTCCCTG
GTTACGATAACCATACCTTTTATGTGTGGTTTGATGCTTTACTTGGATATGTTAGTGCCATTAGTTCTGATGCGACAGAA
CATTCATTAGAAAAATCAATTAATGGAGGATGGCCAGCTGATGTTCATTTAATTGGTAAGGATATTCTGAGATTCCATGC
TGTATATTGGCCTGCAATGCTAATTTCTGCCAAAATGAAAGTTCCAAAAAAGGTTTTTGGGCACGGATTTCTTACAAGAG
AGGGGCAAAAAATGGGTAAAAGCTTAGGAAATGTACTCGACCCTGATTTATTGCTTACAAAATATGGAAATGATCCTGTA
AGGTGGTATCTCATTAAAGACATATCACTAGGAAATGATGGAGATTTTCAAGATAAAAGATTTGTTGACATTATTAATAA
TGACTTAGCTAATACGATTGGTAATCTATTAAATAGAACATCATCTATGTCTAGAAAGTGGTTTAATAATAAAGTTCCAA
ATAAGGAAAAAATTTTCAATGAAAAAAAATTAGAGAATTTTGCCAAAATTGCTGTCGAAAACTATATTTATAACTTTGAT
AATTACAAATTAGATCTAGCAGCTAATGAAGTACTTAGCCTAGCAATTAATACAAATTTGTATTTGAATGATAATCAGCC
ATGGCTGTTAATAAAAGAGAAAGATAATCTACCTTTAGTTAAAGAAATTATCTATAACGTTTTAGAAAGTACACGAATAA
TAGGATTATTATTAATACCTTTATTGCCCGAATTATCTACAAAAATTAATGACCAACTTGGTTCTATATACAGAGAAGAA
ATTCCATGGAAAAAACAATTAATTTGGGGATTATTAGTTAGTAACTCAAGTCTTCCTAAACCCACTCCAATCATAAATAA
ATTGGAGTATGAAGAACATTTATAG

Upstream 100 bases:

>100_bases
ATGATAGTAATCTTTTAGAATTTATTTATTAATAGGATAATAAATACAATAGGTTGTTATTAGTTTAAAAAATATCTGTT
AGTATTAATAAAAAAGCTTT

Downstream 100 bases:

>100_bases
ATTAATAATATTCCTTCCATTATTTATGCTTGGTTGCACCCCAAATGTAATTGATGAAAATAAAGTTATACAAAAAATTG
ACAGTTTAGATATGACTATT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MTFVITTPLYYVNDKPHLGSVYTTIICDSIARYKRLAGEDVIFITGVDEHGLKIQRTANEKGIEPKSHCDEISEVFNNNW
KDWNISFDKFIRTSSKNHEFVVNEFYERVKASDDIYMGVQKGWYCVGCEEFKDNPENSSTYKCPIHQKNLEWKNEENLFF
RLSKYQKEIERIINEPSFIEPIERKNEIINFVSRGLKDFSISRTNVKWGIPVPGYDNHTFYVWFDALLGYVSAISSDATE
HSLEKSINGGWPADVHLIGKDILRFHAVYWPAMLISAKMKVPKKVFGHGFLTREGQKMGKSLGNVLDPDLLLTKYGNDPV
RWYLIKDISLGNDGDFQDKRFVDIINNDLANTIGNLLNRTSSMSRKWFNNKVPNKEKIFNEKKLENFAKIAVENYIYNFD
NYKLDLAANEVLSLAINTNLYLNDNQPWLLIKEKDNLPLVKEIIYNVLESTRIIGLLLIPLLPELSTKINDQLGSIYREE
IPWKKQLIWGLLVSNSSLPKPTPIINKLEYEEHL

Sequences:

>Translated_514_residues
MTFVITTPLYYVNDKPHLGSVYTTIICDSIARYKRLAGEDVIFITGVDEHGLKIQRTANEKGIEPKSHCDEISEVFNNNW
KDWNISFDKFIRTSSKNHEFVVNEFYERVKASDDIYMGVQKGWYCVGCEEFKDNPENSSTYKCPIHQKNLEWKNEENLFF
RLSKYQKEIERIINEPSFIEPIERKNEIINFVSRGLKDFSISRTNVKWGIPVPGYDNHTFYVWFDALLGYVSAISSDATE
HSLEKSINGGWPADVHLIGKDILRFHAVYWPAMLISAKMKVPKKVFGHGFLTREGQKMGKSLGNVLDPDLLLTKYGNDPV
RWYLIKDISLGNDGDFQDKRFVDIINNDLANTIGNLLNRTSSMSRKWFNNKVPNKEKIFNEKKLENFAKIAVENYIYNFD
NYKLDLAANEVLSLAINTNLYLNDNQPWLLIKEKDNLPLVKEIIYNVLESTRIIGLLLIPLLPELSTKINDQLGSIYREE
IPWKKQLIWGLLVSNSSLPKPTPIINKLEYEEHL
>Mature_513_residues
TFVITTPLYYVNDKPHLGSVYTTIICDSIARYKRLAGEDVIFITGVDEHGLKIQRTANEKGIEPKSHCDEISEVFNNNWK
DWNISFDKFIRTSSKNHEFVVNEFYERVKASDDIYMGVQKGWYCVGCEEFKDNPENSSTYKCPIHQKNLEWKNEENLFFR
LSKYQKEIERIINEPSFIEPIERKNEIINFVSRGLKDFSISRTNVKWGIPVPGYDNHTFYVWFDALLGYVSAISSDATEH
SLEKSINGGWPADVHLIGKDILRFHAVYWPAMLISAKMKVPKKVFGHGFLTREGQKMGKSLGNVLDPDLLLTKYGNDPVR
WYLIKDISLGNDGDFQDKRFVDIINNDLANTIGNLLNRTSSMSRKWFNNKVPNKEKIFNEKKLENFAKIAVENYIYNFDN
YKLDLAANEVLSLAINTNLYLNDNQPWLLIKEKDNLPLVKEIIYNVLESTRIIGLLLIPLLPELSTKINDQLGSIYREEI
PWKKQLIWGLLVSNSSLPKPTPIINKLEYEEHL

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=500, Percent_Identity=34.2, Blast_Score=311, Evalue=1e-84,
Organism=Homo sapiens, GI14043022, Length=520, Percent_Identity=25.7692307692308, Blast_Score=158, Evalue=1e-38,
Organism=Escherichia coli, GI1788432, Length=499, Percent_Identity=26.0521042084168, Blast_Score=182, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI212645096, Length=511, Percent_Identity=30.9197651663405, Blast_Score=231, Evalue=9e-61,
Organism=Caenorhabditis elegans, GI71992781, Length=351, Percent_Identity=30.4843304843305, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17540794, Length=554, Percent_Identity=25.4512635379061, Blast_Score=149, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6321610, Length=520, Percent_Identity=33.6538461538462, Blast_Score=258, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6321704, Length=517, Percent_Identity=24.1779497098646, Blast_Score=127, Evalue=4e-30,
Organism=Drosophila melanogaster, GI21358483, Length=489, Percent_Identity=31.0838445807771, Blast_Score=278, Evalue=6e-75,
Organism=Drosophila melanogaster, GI24655311, Length=547, Percent_Identity=27.0566727605119, Blast_Score=177, Evalue=1e-44,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 59570; Mature: 59439

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFVITTPLYYVNDKPHLGSVYTTIICDSIARYKRLAGEDVIFITGVDEHGLKIQRTANE
CEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCC
KGIEPKSHCDEISEVFNNNWKDWNISFDKFIRTSSKNHEFVVNEFYERVKASDDIYMGVQ
CCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEC
KGWYCVGCEEFKDNPENSSTYKCPIHQKNLEWKNEENLFFRLSKYQKEIERIINEPSFIE
CCEEEEECHHHCCCCCCCCEEECCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCC
PIERKNEIINFVSRGLKDFSISRTNVKWGIPVPGYDNHTFYVWFDALLGYVSAISSDATE
HHHHHHHHHHHHHCCCHHCEEECCCEEECCCCCCCCCCEEEEEHHHHHHHHHHHHCCHHH
HSLEKSINGGWPADVHLIGKDILRFHAVYWPAMLISAKMKVPKKVFGHGFLTREGQKMGK
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHH
SLGNVLDPDLLLTKYGNDPVRWYLIKDISLGNDGDFQDKRFVDIINNDLANTIGNLLNRT
HHHHHCCCCCEEEECCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SSMSRKWFNNKVPNKEKIFNEKKLENFAKIAVENYIYNFDNYKLDLAANEVLSLAINTNL
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEECCCEEEEEEECEE
YLNDNQPWLLIKEKDNLPLVKEIIYNVLESTRIIGLLLIPLLPELSTKINDQLGSIYREE
EECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPWKKQLIWGLLVSNSSLPKPTPIINKLEYEEHL
CCHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHCC
>Mature Secondary Structure 
TFVITTPLYYVNDKPHLGSVYTTIICDSIARYKRLAGEDVIFITGVDEHGLKIQRTANE
EEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCC
KGIEPKSHCDEISEVFNNNWKDWNISFDKFIRTSSKNHEFVVNEFYERVKASDDIYMGVQ
CCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEC
KGWYCVGCEEFKDNPENSSTYKCPIHQKNLEWKNEENLFFRLSKYQKEIERIINEPSFIE
CCEEEEECHHHCCCCCCCCEEECCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCC
PIERKNEIINFVSRGLKDFSISRTNVKWGIPVPGYDNHTFYVWFDALLGYVSAISSDATE
HHHHHHHHHHHHHCCCHHCEEECCCEEECCCCCCCCCCEEEEEHHHHHHHHHHHHCCHHH
HSLEKSINGGWPADVHLIGKDILRFHAVYWPAMLISAKMKVPKKVFGHGFLTREGQKMGK
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHH
SLGNVLDPDLLLTKYGNDPVRWYLIKDISLGNDGDFQDKRFVDIINNDLANTIGNLLNRT
HHHHHCCCCCEEEECCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SSMSRKWFNNKVPNKEKIFNEKKLENFAKIAVENYIYNFDNYKLDLAANEVLSLAINTNL
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEECCCEEEEEEECEE
YLNDNQPWLLIKEKDNLPLVKEIIYNVLESTRIIGLLLIPLLPELSTKINDQLGSIYREE
EECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPWKKQLIWGLLVSNSSLPKPTPIINKLEYEEHL
CCHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]