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The map label for this gene is dacB [C]

Identifier: 126696312

GI number: 126696312

Start: 843163

End: 844380

Strand: Direct

Name: dacB [C]

Synonym: P9301_09741

Alternate gene names: 126696312

Gene position: 843163-844380 (Clockwise)

Preceding gene: 126696311

Following gene: 126696314

Centisome position: 51.35

GC content: 23.97

Gene sequence:

>1218_bases
GTGAAAAAAAATATATATTCATTTTTCTTTATTGTTCTTGTATTAGTAACATCTCCTTTCTTAATAAGATATTTAATAGC
AAATCAGAAAATAACTATTTTAAATAGTATTTATTTTAATTTCCCGGGAATAATTACTAAAAACAAAATATGTCCTACTT
ATGATGCTTTATTGAATCAAACGCTTGATAATTCTTTTAGTGTATCAATTATTAATAATAAAGGGGAAATAATAAGTTCC
TACAATGAGGATGTTCCAAGGTTGCCAGCATCTAACCAAAAATTATTCTCATCAGCTTATGTTTTAAGTAAATATAAACT
AAATAATAATTTAAAAACTTCCTTATTTAAAAATAAAAACGATTATTATTTACACGGTCAAGGTGATCCAGATCTTAATT
ATGAAAATATAATCGAACTAATTTCAAATGTTAAAGAAAATAAAATTATTAACTTTAATATTATAGAAATTGATTCGAAA
TTATATTGGCCTAATGGTTGGACAAATACTGATAAACTTTACGAATATGGATCTCCAATTACATCTCTTGCAATAGAAAG
TAATCACAATAAATATAATGATATTTATGCATTAAAAAATTTTATTAAAAATTATTTAAAAAATAAATTTCCAAATTCAA
AAATATATATAAATTTTTTTGATTCAGAAAAAACTTTTTATTTAAAAAATTTTAAAGAGATAAATAAAGTATATTCAACT
CCAATTCTTTCATTATTAACTCTAACAAATTCTGAAAGCCATAATTTTACGGCTGAATCTCTTTTTAAAAATGCCTCAAA
TACATGGAACGATAATAACTATATAAAATTGAAAAGATGGCTTGAAAATAAAGGCCTCCCAGCAACTAATGCATACTTTG
CAGATGCTAGTGGATTGTCTCGAAAAAATAGAATTACAACAAAGTTAGTTGTATTGTTTTTAGATAAAATGAGATATTCT
AATGATTTTAATGCTTATCAATCTACACTTTCAATTACGGGAGTGAGAGGAACGTTAGCTAAAAGATTTGTAAATAGTGA
ATTGTCTGGAAAGTTTTTTGGCAAAACTGGGACTCTTTCGAATGTCTTTGCATTATCTGGCTTTTTATATAAAAATGATA
AGCCAATAATTATAAGCATTATCCAAAATTCTAATAAAATTGATAAAGAAAAAGCTTTTAATTTATTACGTGATCTTTAT
TACTTGAGATCCTGTTAA

Upstream 100 bases:

>100_bases
TGGTTGCTTTTCAAAGAAGAAAATAATAGATATTTTTTTAAAAAAGAATTTGAAATTAAACCCGACAATGAATCTATTTT
TGTAAATTTATAGGTTGAAT

Downstream 100 bases:

>100_bases
AAAAATATTATTTAAAATAATCTTTTAAATCCTTCTCAACAGAGGGGGGTACCATGTGATTAATTTCACCACCAAATTTT
GCAACTTCTTTTACTAAAGA

Product: D-Ala-D-Ala carboxypeptidase 3

Products: NA

Alternate protein names: Peptidase; Serine-Type D-Ala-D-Ala Carboxypeptidase; D-Ala-D-Ala Carboxypeptidase 3 Family Protein

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MKKNIYSFFFIVLVLVTSPFLIRYLIANQKITILNSIYFNFPGIITKNKICPTYDALLNQTLDNSFSVSIINNKGEIISS
YNEDVPRLPASNQKLFSSAYVLSKYKLNNNLKTSLFKNKNDYYLHGQGDPDLNYENIIELISNVKENKIINFNIIEIDSK
LYWPNGWTNTDKLYEYGSPITSLAIESNHNKYNDIYALKNFIKNYLKNKFPNSKIYINFFDSEKTFYLKNFKEINKVYST
PILSLLTLTNSESHNFTAESLFKNASNTWNDNNYIKLKRWLENKGLPATNAYFADASGLSRKNRITTKLVVLFLDKMRYS
NDFNAYQSTLSITGVRGTLAKRFVNSELSGKFFGKTGTLSNVFALSGFLYKNDKPIIISIIQNSNKIDKEKAFNLLRDLY
YLRSC

Sequences:

>Translated_405_residues
MKKNIYSFFFIVLVLVTSPFLIRYLIANQKITILNSIYFNFPGIITKNKICPTYDALLNQTLDNSFSVSIINNKGEIISS
YNEDVPRLPASNQKLFSSAYVLSKYKLNNNLKTSLFKNKNDYYLHGQGDPDLNYENIIELISNVKENKIINFNIIEIDSK
LYWPNGWTNTDKLYEYGSPITSLAIESNHNKYNDIYALKNFIKNYLKNKFPNSKIYINFFDSEKTFYLKNFKEINKVYST
PILSLLTLTNSESHNFTAESLFKNASNTWNDNNYIKLKRWLENKGLPATNAYFADASGLSRKNRITTKLVVLFLDKMRYS
NDFNAYQSTLSITGVRGTLAKRFVNSELSGKFFGKTGTLSNVFALSGFLYKNDKPIIISIIQNSNKIDKEKAFNLLRDLY
YLRSC
>Mature_405_residues
MKKNIYSFFFIVLVLVTSPFLIRYLIANQKITILNSIYFNFPGIITKNKICPTYDALLNQTLDNSFSVSIINNKGEIISS
YNEDVPRLPASNQKLFSSAYVLSKYKLNNNLKTSLFKNKNDYYLHGQGDPDLNYENIIELISNVKENKIINFNIIEIDSK
LYWPNGWTNTDKLYEYGSPITSLAIESNHNKYNDIYALKNFIKNYLKNKFPNSKIYINFFDSEKTFYLKNFKEINKVYST
PILSLLTLTNSESHNFTAESLFKNASNTWNDNNYIKLKRWLENKGLPATNAYFADASGLSRKNRITTKLVVLFLDKMRYS
NDFNAYQSTLSITGVRGTLAKRFVNSELSGKFFGKTGTLSNVFALSGFLYKNDKPIIISIIQNSNKIDKEKAFNLLRDLY
YLRSC

Specific function: Not Involved In Transpeptidation But Exclusively Catalyzes A Dd-Carboxypeptidase And Dd-Endopeptidase Reaction. [C]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.4.16.4; 3.4.99.- [C]

Molecular weight: Translated: 46802; Mature: 46802

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNIYSFFFIVLVLVTSPFLIRYLIANQKITILNSIYFNFPGIITKNKICPTYDALLNQ
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHCCCCEEECCCCCCHHHHHHHH
TLDNSFSVSIINNKGEIISSYNEDVPRLPASNQKLFSSAYVLSKYKLNNNLKTSLFKNKN
HCCCCEEEEEECCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHEEEECCCCCEEHHCCCCC
DYYLHGQGDPDLNYENIIELISNVKENKIINFNIIEIDSKLYWPNGWTNTDKLYEYGSPI
CEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEEEEEECCEEECCCCCCCHHHHHHCCCCH
TSLAIESNHNKYNDIYALKNFIKNYLKNKFPNSKIYINFFDSEKTFYLKNFKEINKVYST
HEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEHHHHHHHHHHHHH
PILSLLTLTNSESHNFTAESLFKNASNTWNDNNYIKLKRWLENKGLPATNAYFADASGLS
HHHHHHEECCCCCCCCCHHHHHHCCCCCCCCCCEEEEEEHHCCCCCCCCCCEECCCCCCC
RKNRITTKLVVLFLDKMRYSNDFNAYQSTLSITGVRGTLAKRFVNSELSGKFFGKTGTLS
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHEEEECCHHHHHHHHHCCCCCCEECCCCCCHH
NVFALSGFLYKNDKPIIISIIQNSNKIDKEKAFNLLRDLYYLRSC
HHHHHHCEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKNIYSFFFIVLVLVTSPFLIRYLIANQKITILNSIYFNFPGIITKNKICPTYDALLNQ
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHCCCCEEECCCCCCHHHHHHHH
TLDNSFSVSIINNKGEIISSYNEDVPRLPASNQKLFSSAYVLSKYKLNNNLKTSLFKNKN
HCCCCEEEEEECCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHEEEECCCCCEEHHCCCCC
DYYLHGQGDPDLNYENIIELISNVKENKIINFNIIEIDSKLYWPNGWTNTDKLYEYGSPI
CEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEEEEEECCEEECCCCCCCHHHHHHCCCCH
TSLAIESNHNKYNDIYALKNFIKNYLKNKFPNSKIYINFFDSEKTFYLKNFKEINKVYST
HEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEHHHHHHHHHHHHH
PILSLLTLTNSESHNFTAESLFKNASNTWNDNNYIKLKRWLENKGLPATNAYFADASGLS
HHHHHHEECCCCCCCCCHHHHHHCCCCCCCCCCEEEEEEHHCCCCCCCCCCEECCCCCCC
RKNRITTKLVVLFLDKMRYSNDFNAYQSTLSITGVRGTLAKRFVNSELSGKFFGKTGTLS
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHEEEECCHHHHHHHHHCCCCCCEECCCCCCHH
NVFALSGFLYKNDKPIIISIIQNSNKIDKEKAFNLLRDLYYLRSC
HHHHHHCEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: Preferential cleavage is (Ac)2-L-Lys-D-Ala D-Ala and Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine [C]

General reaction: Hydrolase; Acting on peptide bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA